BB 451/551 Exam Name______
General Instructions: This exam is worth 100 points total. Read the instructions for each question carefully. If you do not understand the instructions for any section of the exam, raise your hand. Do not spend too much time on any one question. Do not use abbreviations for names of compounds or enzymes that we have not used in class. You may, however, use any abbreviations that have been used by me in class. No one will be permitted to work on the exam longer than 50 minutes. Budget your time appropriately.
Section 1 ______
Section 2 ______
Section 3 ______
Total______
Do not open the exam until you are instructed to begin
This is an example of an old BB 451/551 exam. I strongly urge students NOT to use old exams to study, particularly because topics change from term to term, as can be seen in this exam. I provide the exam only to show you the format your exam will have. I will not answer questions about this exam. (PS: The exam has numerous gibberish questions)
Section I: (20 points total) The statements in this section can be completed by any of the lettered responses following it. Each statement may have more than one answer that is correct, one answer that is correct, or no answers that are correct. Students should clearly circle only those responses that complete the sentence to make a correct statement. Points will be awarded for each circled response that makes a correct statement and for each uncircled response that makes an incorrect statement. For example, the practice question below has three correct answers (b,c,d). You would receive four points if you circled ‘b’,’c’, and ‘d’. You would receive one point if you circled ‘a’ and ‘b’. You would receive no points if you circled only ‘a’. If we have uncertainty about whether or not an answer is marked, it will automatically be counted as a wrongly answered question. Be clear in your markings.
Practice question #A: Oregon State University
A. is a Peruvian factory
B. is located in Corvallis, Oregon
C. has a mascot named Benny Beaver
D. has students from all over the world.
1. With respect to amino acid metabolism,
A. it ends with oxidation of short chain fatty acids in peroxisomes
B. oxidation of saturated fatty acids involves cis intermediates
C. enoyl-CoA isomerase is not needed for oxidation of saturated fatty
acids
D. ketone body synthesis uses the last enzyme of fatty acid synthesis
2. With respect to deoxyribonucleotide metabolism,
A. it uses NDPK to convert all NDPs to dNDPs
B. it uses ribonucleotidereductase to convert dUDT todTDP
C. it is inhibited when dADP concentration is high
D. it can be inhibited by methotrexate inhibition of RNR
3. With respect to DNA exonucleases,
A. leading strand synthesis uses DNA Polymerase III
B. lagging strand synthesis uses DNA Polymerase III
C. absolutely every DNA polymerase acts 5’ to 3’
D. proofreading is 5’ to 3’
4. With respect to glycerophospholipid movement in the body,
A. chylomicron remnants carry fat and cholesterol back from capillaries
to the liver
B. fatty acids travel freely in the bloodstream
C. LDLs are susceptible to oxidation
D. LDLs carry the most fat of the lipoprotein complexes
5. With respect to fat metabolism
A. Acetate is the only component that can be converted into glucose in animals
B. NAD+ and FAD are electron acceptors in fatty acid oxidation
C. Oxidation of fatty acids is not allosterically, hormonally, or enzymatically
regulated
D. ACP is used to carry fatty acids across the mitochondrial membrane
Section II: (50 points total) Each sentence below in this section is missing a word or phrase to complete it. Fill in the blank as appropriate to complete the sentence with a correct statement. Each correctly filled in blank below will be awarded two points.
1. What micromolecule carried by a DNA polymerase allows the enzyme to build a telomere? ______.
2. What enzymatic activity is found in DNA polymerase I, but not in any other enzyme in E. coli? ______.
3. Describe accurately in words what enoyl-CoA isomerase catalyzes
4. What is the name of the lipoprotein complex taken up by cells in receptor mediated endocytosis? ______.
5. What regulatory enzyme named in class determines whether or not nucleosides are made ? ______.
6. What is the exact name of the 3 carbon molecule that donates two carbons to ketone body synthesis (no partial credit)? ______.
7. What is the first citric acid cycle intermediate that propionyl-CoA is converted to? ______.
8. What is the name of the 20 carbon precursor of prostaglandins? ______.
9. The formation of which covalent bonds are catalyzed by the action of DNA polymerases, DNA ligases, and topoisomerases?
10. What enzyme described in class operates at over 6000 rpm? ______.
11. Name the first protein to bind to the E. coli replication origin ______.
12. Name the only regulated enzyme in either fat or fatty acid breakdown ______
13. What is the name of the cellular structure missing in ribosomia? ______
14. Name the molecule produced by catalysis by the only regulated enzyme of fatty acid biosynthesis ______
15. What molecule is the energy source for synthesis of AMP? ______.
16. What is the name of the molecule that is the branch point in de novo purine ribonucleotide synthesis? ______.
17. What atom does NDPK catalyze the removal of? ______.
18. What is the name of the last electron acceptor in fatty acid oxidation? ______.
19. Name the form of RNA discovered by Rosalind Franklin ______.
20. Name the enzyme necessary for making phosphodiester bonds to join together individual RNA fragments ______
21. Ribonucleotidereductase is neutralized by what compound? ______.
22. Specifically where does the compound in the previous question bind to the enzyme?
______.
23. What kinds of molecules inhibit the release of arachidonic acid from membranes? ______.
24. What reactive oxygen-containing molecule is produced as a result of oxidation of very long chain fatty acids? ______.
25. What difference is there between a nucleoside monophosphate and a nucleotide? ______.
Section III: Matching. Place the letter of the term/phrase on the right in the blank before the term on the left that it best matches. Only one letter is appropriate in the blank. Note that there are more terms on the right than there are blanks, so not every term on the right has a best match. Terms on the right may be used once, more than once, or not at all. If we cannot read your writing or if you put two letters in any blank on the left, your answer will be counted wrong automatically. Each correctly matched pair is worth two points.
____ 1. CarnitineA. Steroid catalyst
____2. RibosomesB. Activates acetyl-CoA carboxylase
____ 3. AMPC. Necessary for estradiol synthesis
____4. CDPD. FADH2 producer
____ 5. CyclooxygenaseE. Inhibits own synthesis
____6. Kevin AhernF. Nucleoside
____ 7.AromataseG. Sphingolipid precursor
____ 8. TelomeraseH. Ribose source
____ 9. StatinI. Unsaturated glyerophospholipid
____ 10. PRPPJ. Aspirin target
____ 11. Acyl dehydrogenaseK. dUTPase product
____ 12. Arachidonic acidL. Acetyl-ACPshuttle molecule
____ 13. CitrateM. Competitive inhibitor
____ 14. SerineN. Topoisomerase inhibitor
____ 15. dUMPO. Reverse transcriptase
P. Moves in and out of mitochondria
Q. Electron acceptor in peroxisomes
R. RNR substrate
S. Holds acyl group during fatty acid oxidation
T. Leukotriene precursor
U. Ketone body byproduct
V. Vitamin B12 component
Extra credit (2 points) – Describe precisely using words, not figures, the structure of essential fatty acids in humans.