Laboratory Diagnostics
1 Introduction
Laboratory diagnostics is an important bridge discipline connecting clinical medicine and basic medicine.It is also an independent subject that clinical medicine and laboratory medicine both need to develop.In the last few years,basic theories in medical science and experimental techniques have been developing rapidly.Also laboratory diagnostics have been updating at the speed of 40% per year.
2 Contents and Requirements
This course focuses on the laboratory tests of clinical hematology,clinical biochemistry,clinical immunology,clinical microbiology and molecular biology.Thus when it comes to the teaching part of this discipline,we require that our students master the basic subjects such as histology and pathophysiology, and with good abilities of self-learning and logical thinking.
3 Teaching Methods
As a multidisciplinary and comprehensive curriculum, Laboratory diagnostics combines the theory and practice, in order to let students have a progressive, systematicandcomprehensive understanding on the Laboratory diagnostics. We hope that students could be aware of the importance of laboratory diagnostics to clinical treatment and evaluate test results properly by practical training of routine clinical medical tests.The course will use all the available resources, including outstanding faculty,practical laboratory, synthetic experiment, etc. to elevate student’s ability of experimental manipulation and understanding, identifying and solving both basic science problems and clinical problems.Plastic
4 Recommended Textbook
Laboratory Diagnostics,PekingUniversity Medical Press, Chief Editor Jianzhong Wang.
5 Reference books
1)Laboratory Diagnostics, published by People’s Medical Publishing House
2)Clinical Diagnosis and Management by Laboratory Methods, edited by John Bernard Henry
6 Internet Resources
/
7 Syllabus
The outline is based on the relevant disciplines, five-year plan textbooks.
Teaching / EvaluationLectures / Final exam(85% )
Practice / Laboratory work (15%)
8 Credit hours
This course with 2.5 credits includes 33 credit hours forlectures, 9 credit hours for laboratory classes, and each credit hours lasting for 40 minutes, specifically as follows:
Content / Credit hoursLecture / Blood routine examination / 3
Anemia and hemolysis examination / 3
Bone marrow examination / 3
Coagulation dysfunction examination / 3
Urine and body fluid routine examination / 3
Liver function tests / 3
Renal function tests / 3
Clinical tests of lipid / 3
Analysis of electrolytes,blood gas and lipid / 3
Clinical immunological examination / 3
Clinical etiological examination / 3
Lab / Blood,bone marrow routine examination / 3
Microbiology examination / 3
Biochemistry examination / 3
Chapter One Blood routine examination
Level A
1.RBC,HGB,WBC ,WBC differential count reference values and clinical significance.
2.Differential blood cells count,significance of dysmorphic blood cells.
3.Blood routine examination（HCT,ESR,Ret and so on）.
4.Laboratory diagnosis of anemia, tests of anemia categorization..
Level B
1.The methods of blood routine examination（HCT,ESR,Ret and so on）and their evaluation.
2.The significance of relevant parameters of hematology analyzers.
3.The procedures of laboratory diagnosis of hemolytic anemia.
Level C
1.The detection principles and reference values of hematology analyzers.
Chapter Two clinical hematologic examination
Level A
1.Rules of development of marrow cells,grading by hyperplasia degrees of marrow,the significance of changes about G/E;
2.The basic theories of thrombosis and haemostasis
3.The mechanism of pathology in thrombosis and haemostasis;
4.Application and evaluation of screening tests for thrombosis and haemostasis.
Level B
1.Application and evaluation of common cytochemical stain,choices of diagnostic tests for bleeding and thrombotic diseases.
Level C
1.General concepts of hemopoiesis,morphological characteristics of blood cells,characteristics of blood and bone marrow examination of common blood diseases.
2.Application of special tests for thrombosis and haemostasis.
Chapter Three Urine and body fluid routine examination
Level A
1.Urine routine examination,reference value,clinical significance.
2.Feces examination,occult blood test,reference value,clinical significance.
Level B
1.Methods and significance of serous membrane fluid examination.
Level C
1.Rreference value of test items of urine analyzer,
2.Urine and feces specimen collection and store,
3.Significance of semen, prostatic fluid and vaginal secretion examination.
Chapter Four Clinical etiological examination
Level A
1.Basic rules and significance of etiological laboratory diagnosis.
2.Choice and application of different kinds of test items.
Level B
1.Significance of bacterial resistance and nosocomial infection examination.
Level C
1.Characteristics of etiological examination of bacterial infection,virus infection and fungal infection.
Chapter Five Clinical biochemical examination(1)
Level A
1.Changes of plasma proteins in hepatic disease and their clinical significance
2.Reference value and clinical significance of the test items as TC,TG,Apo and so on.
3.Significance of cTnI.
4.Dynamic change of CPK.
5.Common liver function tests and their clinical significance.
Level B
1.Lipid metabolism disorders and diseases.
Level C
1.Changes of blood glucose in hepatic disease,
2.Common enzymes examination in pancreatic disease.
Chapter Six Clinical biochemical examination(2)
Level A
1.Common renal function tests and their clinical significance.
2.Clinical application of blood gas analysis and acid-base balance tests.
Level B
1.Other renal function tests.
2.Types of acid-base balance disturbance.
Level C
1.Principles and clinical significance of SPI test.
2.Renal endocrinic function tests.
3.Serum K+,Na+,Cl- tests and their clinical significance.
Chapter Seven Clinical immunological examination
Level A
1. clinic application values of routine tests items in clinical immunological examination.
Level B
1.The involved fields of clinical immunological diagnosis,and their corresponding main tests with principles,methods and significance in guide clinical disease diagnosis；parameter value ranges.