Figure Legend:

Supplementary Figure 1. Haplotypes and LD information of COMT gene. (A) LDs among COMT polymorphisms.(B) Haplotypes of COMT polymorphisms. Five SNPs except rs165599 were used for haplotype construction, and there are two blocks. Each box was represented the value of |D’|.

Supplementary Table 1. Frequency of COMT polymorphisms in Korean subjects

Loci / Allele / Position / Amino acid change / Diagnosis / Genotype / MAF / HWE
C/C / C/R / R/R / Total
rs737865 / T>C / Intron1 / SZO / 164 / 161 / 28 / 353 / 0.307 / 0.181
PC / 201 / 156 / 38 / 395 / 0.294 / 0.340
rs740603 / A>G / Intron1 / SZO / 96 / 185 / 67 / 348 / 0.458 / 0.187
PC / 125 / 185 / 83 / 393 / 0.447 / 0.345
rs4633 / C>T / Exon3 / H62H / SZO / 177 / 145 / 26 / 348 / 0.283 / 0.619
PC / 222 / 141 / 30 / 393 / 0.256 / 0.254
rs6267 / G>T / Exon3 / A72S / SZO / 310 / 40 / 4 / 354 / 0.068 / 0.046
PC / 313 / 75 / 6 / 394 / 0.110 / 0.539
rs4680 / G>A / Exon4 / V158M / SZO / 175 / 145 / 30 / 350 / 0.293 / 0.996
PC / 224 / 135 / 34 / 393 / 0.258 / 0.040
rs165599 / A>G / 3' / SZO / 106 / 186 / 56 / 348 / 0.428 / 0.088
PC / 109 / 192 / 92 / 393 / 0.478 / 0.676

SZO: Schizophrenia patient; PC: Population control

C/C, C/R and R/R mean major homozygote, heterozygote and minor homozygote, respectively

Supplementary Table 2. Probe information for COMTSNP genotyping

Loci / ASSAY ID
rs737865 / C___2255420_10
rs740603 / C__11804654_20
rs4633 / C___2538747_20
rs6267 / C__11731855_20
rs4680 / C__25746809_50
rs165599 / C___2255335_10

Supplementary table 3. Association analysis of COMT polymorphisms with the risk of schizophrenia in accordance with gender in Korean population

Loci / Allele / MAF of Male subjects / Chi-square* / P of Chi-square* / Pcorr / MAF of Female subjects / Chi-square* / P of Chi-square* / Pcorr
SZO / PC / SZO / PC
rs737865 / T / 236(67.4%) / 313(69.9%) / 0.5432 / 0.46 / 1 / 253(71.1%) / 245(71.6%) / 0.0277 / 0.87 / 1
C / 114(32.6%) / 135(30.1%) / 103(28.9%) / 97(28.4%)
rs740603 / A / 185(53.8%) / 247(55.6%) / 0.268 / 0.60 / 1 / 192(54.6%) / 188(55.0%) / 0.0126 / 0.91 / 1
G / 159(46.2%) / 197(44.4%) / 160(45.4%) / 154(45.0%)
rs4633 / C / 255(74.6%) / 343(77.3%) / 0.7677 / 0.38 / 1 / 244(68.9%) / 242(70.8%) / 0.2772 / 0.60 / 1
T / 87(25.4%) / 101(22.7%) / 110(31.1%) / 100(29.2%)
rs6267 / G / 329(93.5%) / 395(88.2%) / 6.4233 / 0.01 / 0.06 / 331(93.0%) / 306(90.0%) / 1.9844 / 0.16 / 0.79
T / 23(6.5%) / 53(11.8%) / 25(7.0%) / 34(10.0%)
rs4680 / G / 258(74.1%) / 339(76.7%) / 0.6897 / 0.41 / 1 / 237(67.3%) / 244(70.9%) / 1.0551 / 0.30 / 1
A / 90(25.9%) / 103(23.3%) / 115(32.7%) / 100(29.1%)
rs165599 / A / 178(52.1%) / 225(50.7%) / 0.1452 / 0.70 / 1 / 220(62.2%) / 185(54.1%) / 4.6304 / 0.03 / 0.16
G / 164(47.9% / 219(49.3%) / 134(37.8%) / 157(45.9%)
BL1_ht3 / - / 289(83.5%) / 375(84.5%) / 0.1262 / 0.72 / 1 / 295(82.9%) / 279(81.6%) / 0.1972 / 0.66 / 1
ht3 / 57(16.5%) / 69(15.5%) / 61(17.1%) / 63(18.4%)
BL2_ht1 / - / 116(33.5%) / 163(36.7%) / 0.8628 / 0.35 / 1 / 145(40.7%) / 138(40.4%) / 0.0104 / 0.92 / 1
ht1 / 230(66.5%) / 281(63.3%) / 211(59.3%) / 204(59.6%)