Titles and Legends to Supplementary Figures

Titles and legends to supplementary figures

Supplementary figure 1. Quantitative real-time RT-PCR of MMP-1, MMP-2, MMP-3, and MMP-9 in normal and tumor tissue samples from colorectal cancer patients.Because some data values exhibited asymmetrical distributions, box plots were used. The 10th, 25th, 50th (median), 75th, and 90th percentiles of the variables are shown. *P < 0.0005, Mann–Whitney U test, n=37 for MMP-1 and MMP-3 and n=22 for MMP-2 and MMP-9.

Supplementary figure 2. Quantitative real-time RT-PCR of endothelial markers in tumor tissue samples from 37 colorectal cancer patients divided into four groups according toVEGF and MMP-1 or MMP-3 expression.(a), quantitative real-time RT-PCR of vWF in tumor tissue samples from 37 colorectal cancer patients divided into four groups according to VEGF and MMP-1 expression.*P < 0.05, n=12 for the group with low VEGF and low MMP-1 expression, n=7 for the group with high VEGF and low MMP-1 expression, n=8 for the group with low VEGF and high MMP-1 expression, and n=10 for the group with high VEGF and high MMP-1 expression. (b), quantitative real-time RT-PCR of CD31 in tumor tissue samples from 37 colorectal cancer patients divided into four groups according to VEGF and MMP-1 expression.*P < 0.05. (c), quantitative real-time RT-PCR of vWF in tumor tissue samples from 37 colorectal cancer patients divided into four groups according to VEGF and MMP-3 expression.*P < 0.05, n=12 for the group with low VEGF and low MMP-3 expression, n=6 for the group with high VEGF and low MMP-3 expression, n=8 for the group with low VEGF and high MMP-3 expression, and n=11 for the group with high VEGF and high MMP-3 expression. (d), quantitative real-time RT-PCR of CD31 in tumor tissue samples from 37 colorectal cancer patients divided into four groups according to VEGF and MMP-3 expression.

Supplementary figure 3. Quantitative real-time RT-PCR of endothelial markers in tumor tissue samples from 37 colorectal cancer patients divided into four groups according to VEGF and MMPs expression. The MMP-1, 3, 7 positive groups include the tumors with high expression of all of the three MMPs. The MMP-1, 3, 7 negative groups include the tumors with low expression of at least one of the MMPs (a), quantitative real-time RT-PCR of vWF.P < 0.01, n=15 for the group with low VEGF and low MMP-1, 3, 7 expression, n=11 for the group with high VEGF and low MMP-1, 3, 7 expression, n=3 for the group with low VEGF and highMMP-1, 3, 7 expression, and n=8 for the group with high VEGF and high MMP-1, 3, 7 expression. (b), quantitative real-time RT-PCR of CD31. *P < 0.01.