Supplemental Table S1: Primers Used for the Cloning of Photoreceptors

Supplemental Table S1: Primers used for the cloning of photoreceptors

Name / Sequence (5’ to 3’) / Use
(dT)17-adapter primer
adapter primer / CCGCTCGAGTCGACATCGATTTTTTTTTTTTTTTTT
CCGCTCGAGTCGACATCGA / RT-PCR primer of 3’race
Nested PCR of 3’race
CRY1 deg for
CRY1 deg rev
CRY1 3’ GSP1
CRY1 3’ GSP2
CRY1 5’ GSP1
CRY1 5’ GSP2
CRY1 ATG for
CRY1 Stop rev / ATHAAYGGICCNYTIATHGARTA
TANCCYTCRAAYTGIGGRTTRTCDAT
CTGGGACACACTCTTAGATGCTGAT
AAGTGATGCTCTTGGTTGGCAGTAT
GGCCCAAAGCAACTGCTTGATGCGTA
CCCCAAAATGCAAATGGGGAGAAAGC
ATGTCAGGTGGTAGTGGTTGC
TTACCCAGTTTGAGATAGCCGC / Amplification of gene fragment
“
3’RACE
“
5’RACE
“
Cloning of whole cds
“
CRY2 deg for
CRY2 deg rev
CRY2 3’ GSP
CRY2 ATG for
CRY2 STOP rev / AGCAACTTGAGATTCTTYCCWTGG
GGACTATCCAAACGSTCAAGYTC
CATGGAGAGGGAACTTGTGACAT
ATGGGTAGCAGCAATAAG
CTAGAGGCATTCATCTTCC / Amplification of gene fragment
“
3’ RACE
Cloning of whole cds
“
PHOT1 ATG for
PHOT1 STOP rev / ATGGAAGACGAACCGTCGG
TTAAAAAATATTTGTTTGTAGATCTTGC / Cloning of whole cds
“
PHOT2deg for
PHOT2 deg rev
PHOT2 3’GSP1
PHOT2 3’GSP2
PHOT2ATG for
PHOT2STOP rev / ATHACNGARGTIGTNCARACIGTNAA
TCNCKYTCICKYTCIGCNCKITCCCA
TATCCTAAAAAGTCAGAGCGCAACC
TCTAACTGCAGGCAGGCTTGAAAAG
ATGGAGAAACCAAAGGCAGCTG
TTAGAAAATATCCATATCCATTGC / Amplification of gene fragment
“
3’RACE
“
Cloning of whole cds
“
PHYA deg for1
PHYA deg rev1
PHYA 3’GSP1
PHYA 3’GSP2
PHYA ATG for
PHYA STOP rev / AARCCNTAYGARGTNCCIATGACNGC
TCNCKYTCICKYTCIGCNCKITCCCA
GGTTTGCACTACCCATCCACTGATA
CTCAAGCTTCACGGTTCCTTTTTATG
ATGTCGTCTTCAAGACCCAGTC
CTAGGACCTGATCATATGTGC / Amplification of gene fragment
“
3’RACE
“
Cloning of whole cds
“
PHYB deg for1
PHYB deg rev1
PHYB deg for2
PHYB deg rev2
PHYB for
PHYB rev
PHYB 3’GSP
PHYB 3’GSP
PHYB ATG for
PHYB STOP rev / ATHGAYGTNGGIATHGTNATHGA
CATRTTIARYTGNARICCRAAIGCYTGCAT
GARGARGAYAARAAYGTNGARATHAA
CARAANGCICCDATDATYTGNCC
GTTGGTTGTTTGCCATCACACTTCT
CTAGAGCAAGCATTTACCACCACAA
CTCAGACACCGACAAGTTTCCTTTT
ATGGGAAGTATGTGCAAGCTCTCTT
ATGGCTTCAGCAAGCGGCAAG
TTACCTAGTCAACACTCCTTGC / Amplification of gene fragment
“
“
“
“
“
3’RACE
“
Cloning of whole cds
“

Supplemental Table 2S. PCR primers used for RT-PCR analysis.

Name / Sequence (5’ to 3’) / reference / GenBank Database
CRY1 for
CRY1 rev / CAACCTCCATTGCGTTTCCTG
AGGCACTTCAGCTCTGCTTGG / This study / AB743569
CRY2 for
CRY2 rev / ACTTGCCGATCCCAAAGGTG
TCTGGTAGTGGCCGTTCTTGTG / This study / AB743570
PHOT1 for
PHOT1 rev / AAACCTAGACGCCGCTCTTTC
CGTCCTCAGAAGCTCCAAACTC / This study / AB743568
PHOT2 for
PHOT2 rev / AAGCCCGGCAGACAAACTC
TTCAGTGCGGTCCCAACTATC / This study / AB743567
PHYA for
PHYA rev / CGGAAAATGATCGAGGGTACTG
CTCAACCATTTCCAGGTCCAAG / This study / AB743571
PHYB for
PHYB rev / GTTCCTTGAGACTAGTGCTGCTTG
CCTTCTCAAGCTCCAAAGAACC / This study / AB743572
PAL for
PAL rev / TTGAAGCTCATGTCTTCCAC
CAAGTTCTCCTCCAAATG / Almeidi (2007) / AF237955, X68126, AF036948
CHS for
CHS rev / GCAACACCACCCAACTGTATG
ACACATGCGCTGGAATTTCTC / Miyawaki(2012) / AB665441
CHI for
CHI rev / TTTTCAATGGCTTTCGCTTCTG
GTGACAATGATACTACCGCTGACG / Miyawaki (2012)
Salvatierra (2010) / HM026182
F3H for
F3H rev / GTGCGCCACCGTGACTACTC
ATGCCTTTGTCAATGCCTCC / Salvatierra (2010) / HM026183
DFR for
DFR rev / GGGTGGTGTTTACATCTTCGG
CTGCTTGCTCGGCTAGAGTTT / Miyawaki (2012)
Salvatierra (2010) / HM026184
ANS for
ANS rev / GACTTGTCCATTTGGCCTC
CCCCCTCAGTTCCTTAGCATACTC / Almeidi (2007) / AY695817
FGT for
FGT rev / ATCGTGGCTTGACAAACAGAA
TGACCACAAGAATGGAACCCTA / Salvatierra (2010) / HM026186
GAPDH for
GAPDH rev / TCCATCACTGCCACCCAGAAGACTG
AGCAGGCAGAACCTTTCCGACAG / Miyawaki (2012)
Salvatierra (2010) / AB363963
phot2i for
phot2i rev / GCTCGACAAAAGGAGAAGG
AAATCTCCCCCGAGATGC / This study / AB743567