1.Name and Personal Data

Curriculum VitaeYu Lan, Ph.D., 02/2017

1.NAME AND PERSONAL DATA:

Yu Lan, Ph.D.

Associate Professor (Research)

Division of Plastic Surgery

Division of Developmental Biology

Cincinnati Children’s Hospital Medical Center

3333 Burnet Avenue, MLC 7007

Cincinnati, OH 45229

Telephone 513-803-7842

email:

2. EDUCATION

B. S. (Biology), 1983, Sichuan University, Chengdu, China

M. S. (Genetics), 1986,Chinese Academy of Sciences, Beijing, China

Ph. D. (Plant Sciences), 1992, University of Maine, Orono, Maine, USA

Postdoc (DrosophilaDevelopmental Genetics. Advisor: Dr. Michael Weir), Wesleyan University, Middletown, CT. 1992 –1994.

Postdoc (Mouse Developmental Genetics. Advisor: Dr. Thomas Gridley). The Jackson Laboratory, Bar Harbor, ME. 1995 – 1998.

3. ACADEMIC APPOINTMENTS

1986 - 1987Research Scientist, Institute of Genetics, Chinese Academy of Sciences, Beijing, China

1999 - 2010Research Assistant Professor, Center for Oral Biology and Department of Biomedical Genetics, University of Rochester, Rochester, NY

2010 - 2011 Research Associate Professor, Center for Oral Biology and Department of Biomedical Genetics, University of Rochester, Rochester, NY

2011 - Associate Professor, Division of Plastic Surgery & Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH

2011-Associate Professor (Affiliate), Department of Surgery, University of Cincinnati college of Medicine, Cincinnati, OH

4.AWARDS AND HONORS

NIH/NCI Postdoctoral Trainee, 07/1996 – 06/1998.

Chair, Palate Development Oral Session, American Association of Dental Research Annual Meeting,03/2004.

Chair, Craniosynostosis and Palatogenesis Oral Session, International Association of Dental Research Annual Meeting,07/2010.

Cincinnati Children’s Hospital Medical Center Trustee Award, 01/01/2014 – 12/31/2015

Chair, Genetic Insights into Craniofacial Development and DiseaseOral Session. Society for Craniofacial Genetics and Developmental Biology 39th annual meeting. 08/2016

5. MEMBERSHIP IN PROFESSIONAL ORGANIZATIONS

American Cancer Society, member, 1995-1998

Society for Developmental Biology, member, 2002 – present

International/American Association for Dental Research, member, 2003 – present

Society for Craniofacial Genetics and Developmental Biology, member, 2011- present

6. SERVICE

1. National/International Distinguished Activities:

Chair, Palate Development Oral Session, American Association of Dental Research Annual Meeting, 03/2004.

Chair, Craniosynostosis and Palatogenesis Oral Session, International Association of Dental Research Annual Meeting, 07/2010.

Chair, Genetic Insights into Craniofacial Development and DiseaseOral Session. Society for Craniofacial Genetics and Developmental Biology 39th annual meeting. 08/16

1. Manuscript review:

Ad hoc reviewer for: Development; Developmental Biology. Average 2 manuscripts per year.

1. Participation in local activities that benefit the institution:

Active participant of group meetings and discussions on bed-to-bench translational research topics in the Craniofacial Biology Research Group.

Active participant at the CCHMC Annual Craniofacial Skin Sciences Symposium.

7. TEACHING

Contributing extensively to ongoing training of graduate students, postdoctoral fellows, and research assistants in research design, experimental techniques, and data analyses.

Teaching and training undergraduate students in summer research, 2012; 2014; 2015.

Teaching French exchange students in 2015, 2016

Training first-year graduate students during their laboratory rotations, 2012 – 2016.

8. GRANTS AND CONTRACTS

Active:

(1). NIH R03 DE023864 “Golgb1 in Palate Development”

Role: Principal Investigator, 20% effort. 12/03/2013 - 11/30/16

Peer Reviewed.

(2).NIH R01 DE018401 “Molecular Patterning of Mammalian Dentition”

Role: Co-Investigator, 40% effort.09/12/13 – 06/30/18

Peer Reviewed.

(3). Shriners Hospitals for Children Grant #85900 “Molecular Regulation of Palate Development”

Role: Co-Investigator, 30% effort.01/01/2017 – 12/31/2021

Peer Reviewed.

Pending:

(4)NIH R01 DE027046-01 “Mandible Development”

Role: Co-Investigator, 20% effort. 07/01/2017 -06/30/2022

Peer Reviewed.

Completed:

(1)NIH R01 DE015207 “Genetic Basis of Cleft Lip and Palate”

Role: Co-Investigator, 30% effort

04/01/2003 – 01/31/2013

Peer Reviewed.

(2).NIH R01 DE013681 “Molecular Genetic Analysis of Craniofacial Development”

Role: Co-Investigator, 40% effort.04/01/00 – 06/30/15

Peer Reviewed.

(3). Cincinnati Children’s Hospital Medical Center Trustee Award

“Golgb1 in Craniofacial Development”

Role: Principal Investigator.01/01/2014 – 12/31/2015

Peer Reviewed.

9. PEER REVIEWED PUBLICATIONS

(1)Anathan, J., Baler, R., Morrissey, D., Zou, J., Lan, Y., Weir, M., Voellmy, R. (1993). Synergistic activation of transcription is mediated by the N-terminal domain of Drosophila fushi tarazu homeoprotein and can occur without DNA binding by the protein. Molecular and Cellular Biology, 13:1599-1609. PMID: 8095092

(2) Cai, J., Lan, Y., Appel, L.F., Weir, M. (1994). Dissection of the Drosophila Paired protein: Functional requirements for conserved motifs. Mechanisms of Development, 47:139-150. PMID: 7811637

(3) *Miskiewicz, P., *Morrissey, D.,* Lan, Y., Raj, L., Kessler, S., Fujioka, M., Goto, T., Weir, M. (1996). Both the paired domain and homeodomain are required for in vivo function of Drosophila Paired. Development, 122:2709-2718. (* co-first authors)PMID: 8787745

(4) Casirola, D.M., Lan, Y., Ferraris, R.P. (1997). Effects of changes in calorie intake on intestinal nutrient uptake and transporter mRNA levels in aged mice. Journal of Gerontology, 52:B300-3109. PMID: 9402931

(5) Lan, Y., Jiang, R., Shawber, C., Weinmaster, G., Gridley, T. (1997). The Jagged2 gene maps to chromosome 12 and is a candidate for the lgl and sm mutations. Mammalian Genome 8:875-876. PMID: 9341252

(6)Lan, Y., Fujioka, M., Polsgrove, R., Miskiewicz, P., Morrissey, D., Goto, T., Weir, M. (1998). Plasticity of Drosophila Paired function. Developmental Genetics, 23:45-55.PMID: 9706693

(7) Bell, S., Lan, Y., Jiang, R., Gridley, T. (1998). Exclusion of Jagged2 as a candidate for the legless gene. Mammalian Genome 9:778-779. PMID: 9716668

(8)*Jiang, R., * Lan, Y., Chapman, H.D., Shawber, C., Norton, C.R., Serreze, D.V., Weinmaster, G., Gridley, T. (1998). Defects in limb, craniofacial and thymic development in Jagged2 mutant mice. Genes & Development 12:1046-1057. (* co-first authors)PMID: 9531541

(9) Jiang, R., Lan, Y., Norton, C.R., Gridley, T. (1998). The Slug gene is not essential for mesoderm or neural crest development in mice. Developmental Biology 198:277-285.PMID: 9659933

(10) Lanford, P.J., Lan, Y., Jiang, R., Lindsell, C., Weinmaster, G., Gridley, T., Kelley, M.W. (1999). Notch signaling mediates Hair cell development in mammalian cochlea. Nature Genetics 21:289-292. PMID: 10080181

(11) McCright, B., Gao, X., Lozier, J., Lan, Y., Shen, L., Maguire, M., Herzlinger, D., Weinmaster, G., Jiang, R., Gridley, T. (2001). Defects in development of the kidney, heart and eye vasculature in mice homozygous for a hypomorphic Notch2 mutation. Development 128:491-502.PMID: 11171333

(12) Lan, Y., Kingsley, P. D., Cho, E. S., Jiang, R. (2001). Osr2, a new mouse gene related to Drosophila odd-skipped exhibits dynamic expression patterns during craniofacial, limb, and kidney development. Mechanisms of Development 107:175-179.PMID: 11520675

(13) Carver, E. A., Jiang, R., Lan, Y., Oram, K. F., Gridley, T. (2001). The mouse Snail gene encodes a key regulator of the epithelial-mesenchymal transition. Molecular and Cellular Biology 21:8184-8188.PMID: 11689706

(14) Bush, J. O., Lan, Y., Maltby, K. M., Jiang, R. (2002). Isolation and developmental expression analysis of Tbx22, the mouse homolog of the human X-linked cleft palate gene. Developmental Dynamics 225:322-326.PMID: 12412015

(15) Krebs, L. T., Iwai, N., Nonaka, S., Welsh, I. C., Lan, Y., Jiang, R., Saijoh, Y., O’Brien, T. P., Hamada, H., Gridley, T. (2003). Notch signaling regulates left-right asymmetry determination by inducing Nodal expression. Genes & Development 17:1207-1212.PMID: 12730124

(16) Lan, Y., Ovitt, C. E., Cho, E.-S., Maltby, K. M., Wang, Q., Jiang, R. (2004). Odd-skipped related-2 (Osr2) encodes a key intrinsic regulator of secondary palate growth and morphogenesis. Development 131:3207-3216.PMID: 15175245

(17) Bush, J. O., Lan, Y., Jiang, R. (2004). The cleft lip and palate defects in the Dancer mutant mice result from gain of function of the Tbx10 gene. Proceedings of the National Academy of Sciences USA 101:7022-7027.PMID: 15118109

(18) Wang, Q., Lan, Y., Cho, E.-S., Maltby, K. M., Jiang, R. (2005). Odd-skipped related 1 (Odd1) is an essential regulator of heart and urogenital development. Developmental Biology 288:582-594.PMID: 16223478

(19) Liu, H., Liu, W., Maltby, K. M., Lan, Y., Jiang, R. (2006). Identification and developmental expression analysis of a novel homeobox gene closely linked to the mouse Twirler mutation. Gene Expression Patterns (Mechanisms of Development) 6:632-636.PMID: 16426902

(20) Lan, Y., Ryan, R. C., Zhang, Z., Bush, J. O., Maltby, K. M., Bullard, S. A., Lidral, A. C., Jiang, R. (2006). Expression of Wnt9b and activation of canonical Wnt signaling during midfacial morphogenesis in mice. Developmental Dynamics 235:1448-1454.PMID: 16496313

(21) Casey, L. M., Lan, Y., Cho, E.-S., Maltby, K. M., Gridley, T., Jiang, R. (2006). Jag2-Notch1 signaling regulates oral epithelial differentiation and palate development. Developmental Dynamics 235:1830-1844.PMID: 16607638

(22) Chen, J., Bush, J.O., Ovitt, C.E., Lan, Y., Jiang, R. (2007). The TGFβ pseudoreceptor gene Bambi is dispensable for mouse embryonic development and postnatal survival. Genesis 45:482-486.PMID: 17661381

(23) #Lan, Y., Wang, Q., Ovitt, C.E., #Jiang, R. (2007). A unique mouse strain expressing Cre recombinase for tissue-specific analysis of gene function in palate and kidney development. Genesis, 45:618-624. (# corresponding author)PMID: 17941042

(24) Liu, W., Lan, Y., Pauws, E., Meester-Smoor, M. A., Stanier, P., Zwarthoff, E. C., Jiang, R. (2008). The Mn1 transcription factor acts upstream of Tbx22 and preferentially regulates posterior palate growth in mice.Development, 135(23):3959-3968. PMID: 18948418.

(25) Gao, Y., Lan, Y., Ovitt, C. E., Jiang, R. (2009). Functional equivalence of the zinc finger transcription factors Osr1 and Osr2 in mouse development. Developmental Biology328:200-209. PMID: 18948418.

(26) Xiong, W., He, F., Morikawa, Y., Yu, X., Zhang, Z., Lan, Y., Jiang, R., Cserjesi, P., Chen, Y. (2009). Hand2 is required in the epithelium for palatogenesis in mice. Developmental Biology 330:130-141.PMID:19341725

(27) Chen, J., Lan, Y., Gao, Y., Baek, J.-A., Jiang, R. (2009). Wnt/-catenin signaling plays an essential role in activation of odontogenic mesenchyme during early tooth development. Developmental Biology 334:174-185.PMID: 19631205

(28) *Zhang, Z., *Lan, Y., Chai, Y., Jiang, R. (2009). Antagonistic actions of Msx1 and Osr2 pattern mammalian teeth into single row. Science 323:1232-1234. (*co-first author)PMID: 19251632. (* co-first authors).

(29) Lan, Y., Jiang, R. (2009). Sonic hedgehog signaling regulates reciprocal epithelial-mesenchymal interactions controlling palatal outgrowth. Development 136:1387-1396. PMID: 19304890.

(30) Liu, W., Watson, S. S., Lan, Y., Keene, D. R., Ovitt, C. E., Liu, H., Schweitzer, R., Jiang, R. (2010). The atypical homeodomain transcription factor Mohawk controls tendon morphogenesis. Molecular and Cellular Biology 30:4797-4807. PMID: 20696843

(31) Baek, J-A., Lan, Y., Liu, H., Maltby, K.M., Mishina, Y., Jiang, R. (2011). Bmpr1a signaling plays critical roles in palatal shelf growth and palatal bone formation. Developmental Biology 350:520-531.PMID: 21185278

(32) Gao, Y., Lan, Y., Liu, H., Jiang, R. (2011). The zinc finger transcription factors Osr1 and Osr2 control synovial joint formation. Developmental Biology 352:83-91. PMID: 21262216

(33) Lan, Y., Liu, H., Ovitt, C. E., Jiang, R. (2011). Generation of Osr1 conditional mutant mice. Genesis, 49:419-422. PMID: 21462293

(34) Zhou, J., Gao, Y., Zhang, Z., Zhang, Y., Z., Liu, Maltby, K.M., Lan, Y., Jiang, R. (2011). Osr2 acts downstream of Pax9 and interacts with both Msx1 and Pax9 to pattern the tooth developmental field. Developmental Biology 353:344-353.PMID: 21420399.

(35) Jia, S., Zhou,J.,Gao, Y., Baek, J-A, Martin, J.F., Lan, Y., and Jiang, R. (2013) Roles of Bmp4 during tooth morphogenesis and sequential tooth formation. Development 140:423-432. PMID: 23250216.

(36) Zhu, S., Chen, K., Lan, Y., Zhang, N., Jiang, R., Hu, J. (2013). Alendronate protects against articular cartilage erosion by inhibiting subchondral bone loss in ovariectomized rats. Bone 53:340-349. PMID: 23291609.

(37) Liu, H, Lan, Y., Xu, J., Chang, C.F., Brugmann, S.A. and Jiang,R. (2013) Odd-skipped related-1 controls neural crest chondrogenesis during tongue development. Proceedings of the National Academy of Sciences USA 110:18555-18560.PMID: 24167250.

(38) Zhou, J., Gao Y, Lan Y. Jia S and Jiang R. (2013) Pax9 regulates a molecular network involving Bmp4, Fgf10, Shh signaling and the Osr2 transcription factor to control palate morphogenesis. Development 140:4709-4718.PMID: 24173808

(39) Lan, Y., Jia, S. and Jiang, R. (2013) Molecular patterning of the mammalian dentition. Seminars in Cell and Developmental Biology 25-26:61-70. PMID:

24355560.

(40) Zhou, H., Zou, S., Lan, Y., Fei, W., Jiang, R., Hu, J. (2014). Smad7 modulates TGF-beta signaling during cranial suture development to maintain suture patency. J. Bone Min. Res. 29:716-724. PMID:23959527

(41) Xu, J., Liu, H., Park, J. S., Lan, Y., Jiang, R. (2014). Osr1 acts downstream of and interacts synergistically with Six2 to maintain nephron progenitor cells during kidney organogenesis. Development 141: 1442-1452.PMID:24598167

(42) Liu, H., Xu, J., Liu, C-F., Lan, Y., Wylie, C., Jiang, R. (2015). Whole transcriptome expression profiling of mouse limb tendon development by using RNA-seq. Journal of Orthopaedic Research 33:840-848.PMID:25729011

(43) Kwon, H-J, Park, E-K, Jia, S, Liu, H, Lan, Y., Jiang, R. (2015). Deletion of Osr2 partially rescued tooth development in Runx2 mutant mice. Journal of Dental Research 94:1113-1119.PMID:25916343

(44) #Lan, Y., Xu, J., #Jiang, R. (2015). Cellular and Molecular Mechanisms of Palatogenesis. Current Topics in Developmental Biology (Craniofacial Development) 115:59-84. (# corresponding author)PMID:26589921

(45) Xu, J., Liu, H., Lan, Y., Aronow, B.J., Kalinichenko, V.V., Jiang, R. (2016). A Shh-Foxf-Fgf18-Shh molecular circuit regulating palate development. PLOS Genetics 12(1):e1005769. doi: 10.1371/journal.pgen.1005769.PMID:26745863

(46) #Lan, Y., Zhang, N., Liu, H., Xu, J., Jiang, R. (2016). Golgb1 regulates protein glycosylation and is crucial for mammalian palate development. Development.143(13):2344-55(#solecorresponding author)PMID:27226319

(47) Xu, J., Liu, H., Chai, O.H., Lan, Y., Jiang, R. (2016). Osr1 Interacts Synergistically with Wt1 to Regulate Kidney Organogenesis. PLOS One 11(7):e0159597. doi: 10.1371/journal.pone PMID:27442016

(48)Jia, S., Kwon, H-J.E., Lan, Y., Zhou, J., Liu, H., Jiang, R.(2016). Bmp4-Msx1 signaling and Osr2 control tooth organogenesis through antagonistic regulation of secreted Wnt antagonists. Developmental Biology420:110-119. doi: 10.1016/j.ydbio.2016.10.001.

(49)Kwon, H.-J.E., Jia, S., Lan, Y., Liu, H., Jiang, R. (2017). Activin and Bmp4 signaling converge on Wnt activation during odontogenesis. Journal of Dental Research, in press.

(50)Li, C., Lan, Y., Jiang, R. (2017). Molecular and Cellular Mechanisms of Palate Development. Journal of Dental Research, in press.

(51)Fu, X., Xu, J., Chaturvedi, P., Liu, H., Jiang, R., Lan, Y. (2017). Identification of Osr2 transcriptional target genes in palate development. Submitted.

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