Enzyme activity LOCK AND KEY HYPOTHESIS MODEL

Materials

·  2 markers

·  3 index cards

·  1 piece of construction paper

·  scissors

Procedure

  1. Label index cards 1, 2, and 3 in the top right corner.
  2. On a piece of scrap paper design an original lock and key model of an enzyme and substrate.
  3. On card #1 draw your simple, original substrate shape on the left half of the card.
  4. Cut out the substrate and trace portion as active site of enzyme on remaining right side of index card.
  5. Finish drawing the shape of your original enzyme.
  6. Cut the enzyme.
  7. Use different markers color the substrate and enzyme.
  1. On index cards #2 and #3 trace the enzyme and substrate shapes.
  2. Cut out the enzymes and substrates.
  3. Color the enzymes and substrates from 9 the same colors as used in 7.
  1. Position the construction paper hot dog style on the table.
  2. Title the paper LOCK AND KEY HYPOTHESIS (be as creative as you like).
  1. Place a substrate molecule on the far left of the paper (orientate so that it will fit with enzyme).
  2. Write a + symbol next to the substrate.
  3. Place an enzyme next to the + symbol.
  4. Label the substrate and enzyme above the picture.
  5. Label lock and key under the appropriate pictures.
  6. Draw an arrow pointing to the enzyme area that the substrate will fit into. Label it the active site.
  7. Draw an arrow to the right of the enzyme.
  1. Take a second pair of enzyme/substrate cut-outs; arrange them fitting snugly together in the same orientation to the right of the arrow.
  2. Label this pair the Enzyme Substrate Complex.
  3. Draw an arrow to the right of the complex.
  1. Position third substrate cut in half representing the products leaving the third enzyme.
  2. Label the halved substrate products.
  3. Label the enzyme as reusable and draw an arrow back to the beginning.
  4. Glue all items to the construction paper after it has been checked by your teacher.