Molecular Biology Reports

In vivo study of hepatitis B vaccine effects on inflammation

and metabolism gene expression

Authors:

Heyam Hamza*, Jianhua Cao, Xinyun Li and Shuhong Zhao

Address:

Key lab of Animal Genetics, Breeding and reproduction-college of Animal Science & Tchnology, Ministry of education,HuazhongAgriculturalUniversity, Wuhan 43370, PR China.

*Correspondence to: Heyam Hamza

E-mail:

Supplementary table 1

The 144 annotated genes in mice liver following HepatitisB vaccine

Gene Description / Gene I.D / a
Log FC / P-value
serum amyloid A 2
orosomucoid 2
serum amyloid A 1
serum amyloid A 3
alpha-2-macroglobulin
proteinase 3 / NM_011314
NM_011016
NM_009117
NM_011315
NM_175628
NM_011178 NM_001044384
NM_001045481
NM_133753
NM_008176
NM_001033450
NM_013623
NM_001159394
NM_133990
NM_145594
NM_013650 NM_009114
NM_01131
NM_001039150 NM_001142681 NM_011318
NM_009139
NM_013497
NM_133889
NM_024439
NM_008768
NM_016974
NM_007805 NM_012055
NM_023627
NM_011584
NM_009127
NM_029796
NM_011044
NM_007876
NM_130864
NM_146230
NM_025436
NM_020010
NM_146041
NM_001130412
NM_008489
NM_008898
NM_007824
NM_010191 / -6.9651
-6.4863
-5.6516
-5.2574
-4.4732
-4.4271
-3.5893
-3.3493
-2.24
-2.2311
-2.0656
-1.9983
-1.803
-1.0514
-1.60683
-2.52025
-2.39604
-1.0329
-1.3714
-0.47127
-1.0329
-2.15872
-0.451183
0.642217
-0.573867
-1.300667
-3.8015
-2.9285
-2.5681
-2.2478
-2.1998
1.91193
-1.697
-1.4856
-1.4744
1.3614
1.16473
-1.1393
-1.1208
-2.12465
-1.37934
-1.55047
-0.93757
-1.38745
-0.96673 / 4.63538E-05
1.82E-05
0.000786769
0.000104971
4.69E-06
0.001764889
0.001451174
0.000109904
0.0031
0.0008
0.0002
0.0017
0.00741993
0.0005
0.0174
0.0068
0.01669029
0.0049
0.0078
0.004
0.0049
0.0041
0.0117
0.0002
0.003016
0.003017
0.001171899
0.001693179
0.00309322
0.0022
0.0011
0.0013
0.0028
0.0021
0.0007
0.0008
0.0002
0.0007
0.001
0.0169
0.0055
0.0039
0.013
0.0329
0.0072
tissue inhibitor of metalloproteinase 1
interferon activated gene 203
ERBB receptor feedback inhibitor 1
chemokine (C-X-C motif) ligand 1
interferon activated gene 205
orosomucoid 3
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, ze
interleukin 13 receptor, alpha 1
fibrinogen-like protein 1
S100 calcium binding protein A8 (calgranulin A)
S100 calcium binding protein A9 (calgranulin B)
serum amyloid P-component
macrophage scavenger receptor 1 CD44 antigen
CD44 antigen
chitinase domain containing 1
chemokine (C-C motif) ligand 6
cAMP responsive element binding protein 3
BSD domain containing 1
histocompatibility 47
orosomucoid 1
D site albumin promoter binding protein
cytochrome b-561
asparagine synthetase
myo-inositol 1-phosphate synthase A1
nuclear receptor subfamily 1, group D, member 2
stearoyl-Coenzyme A desaturase 1
leucine-rich alpha-2-glycoprotein 1
phosphoenolpyruvate carboxykinase 1, cytosolic
dipeptidase 1 (renal)
acetyl-Coenzyme A acyltransferase 1A
acetyl-Coenzyme A acyltransferase 1B
sterol-C4-methyl oxidase-like
cytochrome P450, family 51
GDP-mannose 4, 6-dehydratase
lipin 1
lipopolysaccharide binding protein
P450 (cytochrome) oxidoreductase
cytochrome P450, family 7, subfamily a, polypeptide 1
farnesyl diphosphate farnesyl transferase 1
epoxide hydrolase 2, cytoplasmic
myo-inositol 1-phosphate synthase A1
serine (or cysteine) peptidase inhibitor, clade A, member 3N
lanosterol synthase
UDP-glucose ceramide glucosyltransferase
citrate synthase
hemochromatosis
arachidonate 12-lipoxygenase
lysophosphatidylglycerol acyltransferase 1
tribbles homolog 3 (Drosophila)
monoglyceride lipase
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2
fucokinase
transketolase
choline kinase beta
ketohexokinase
UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
phosphoglycolate phosphatase
3-ketodihydrosphingosine reductas
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1
aldo-keto reductase family 1, member C12
Ceruloplasmin
metallothionein 2
metallothionein 1
carbonic anhydrase 3
potassium channel, subfamily T, member 2
FK506 binding protein 11
arrestin domain containing 3
ATPase, H+ transporting
nucleobindin 2
solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3
guanylate cyclase 2c
potassium inwardly-rectifying channel, subfamilymember 9
Crystallin,betaB3
RIKEN cDNA
NEDD4 binding protein 2-like1
Fam134b
enolase 1, alpha non-neuron /// predicted gene 5506
enolase 1, alpha non-neuron
hexamethylene bis-acetamide inducible 1
esterase 22
caseinolytic peptidase X (E.coli)
major facilitator superfamily domain containing 7C
signal recognition particle 54a /// signal recognition particle 54b /// signal r
splicing factor, arginine/serine-rich 15
enolase 1, alpha non-neuron /// predicted gene
RIKEN cDNA 9030624J02 gene
RIKEN cDNA C630043F03 gene
homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like d
small nucleolar RNA host gene (non-protein coding) 1
RIKEN cDNA A430091L06 gene
enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
peptidylprolyl isomerase B
v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian)
RNA polymerase II associated protein 3
angiogenin, ribonuclease
SPARC related modular calcium binding 2
de-etiolated homolog 1 (Arabidopsis
similar to NFIL3/E4BP4 transcription factor /// nuclear factor, interleukin 3, r
golgi SNAP receptor complex member 2
Suppressor of zeste 12 homolog (Drosophila)
cyclin M3
CDK5 regulatory subunit associated protein 3
THAP domain containing 4
sel-1 suppressor of lin-12-like (C. elegans)
RAR-related orphan receptor gamma
similar to SMAP1 /// stromal membrane-associated protein 1
ribophorin I
GTP binding protein 2
ataxin 2 binding protein 1
cyclin D2
actinin alpha 2
vacuolar protein sorting 28 (yeast)
spermatogenesis associated 5
plasma membrane proteolipid
ubiquitin family domain containing 1
ribosome binding protein 1
DDB1 and CUL4 associated factor 5
parathyroid hormone 2 receptor
coatomer protein complex, subunit beta 2 (beta prime)
ankyrin 3, epithelial
connective tissue growth factor
NIMA (never in mitosis gene a)-related expressed kinase 3
MLX interacting protein-like
similar to neuroblastoma ras oncogene /// neuroblastoma ras oncogene
erythrocyte protein band 4.1-like 3
protein phosphatase 3, catalytic subunit, beta isoform
zinc finger with KRAB and SCAN domains 1
leucine rich repeat (in FLII) interacting protein 1
nuclear fragile X mental retardation protein interacting protein 1
eukaryotic translation initiation factor 1A, Y-linked
family with sequence similarity 134, member B
RAS-related C3 botulinum substrate 1
chromodomain helicase DNA binding protein 4
Zinc finger, MYND domain containing 19
poly(rC) binding protein 2
zinc finger protein 292
DNA-damage inducible protein 2 / NM_007940
NM_023627
NM_009252
NM_146006
NM_011673
NM_026444
NM_010424
NM_007440
NM_001134829
NM_175093
NM_011844
NM_008256
NM_172283
NM_009388
NM_007692
NM_008439
NM_020283
NM_001024955
NM_025954
NM_027534
NM_145942
NM_013777
NM_001042611
NM_008630
NM_013602
NM_007606
NM_001081027
NM_024169
NM_001042591
NM_175406
NM_001130479
NM_054055
NM_001127318
NM_008429
NM_001159650
NM_001475679
NM_133898
NM_001025388
NM_023119
NM_138753
NM_133660
NM_001044389
NM_145447
NM_001100109
NM_178923
NM_001025388 NM_027815
NR_027923
NM_022331
NR_002896
_
NM_023737
NM_011149
NM_010658
NM_001034851
NM_028003
NM_001161731
NM_022315
NM_029585
NM_017373
NM_019650
NM_001163018
NM_001039551
NM_030248
NM_025920
NM_001039089
NM_011281
NM_028534
NM_133933
NM_001145979
NM_021477
NM_009829
NM_033268
NM_025842
NM_001163511
NM_026385
NM_138589
NM_024281
NM_177267
NM_139270
NM_015827
NM_009670
NM_010217
NM_001162947
NM_021455
NM_010937
NM_013813
NM_008914
NM_029869
NM_001111311
NM_013745
NM_025437
NM_001034851
NM_009007
NM_145979
NM_026021
NM_001103165
NM_013889
NM_001017966 / 1.340533
-2.2477
-1.0914
-0.36873
-0.41677
0.57508-0.576993
-0.493587
-0.911363
-1.227993
1.090633
1.090633
0.5026
0.552733
0.392833
0.693
-0.4678
0.63785
0.64805
0.789993
-0.629967
0.597743
-0.5869
-4.4812
-2.6104 2.57932
-1.8406
-1.3624
-1.01339
-1.70419
-2.67907
-1.4685
-1.4461
0.376437
-2.2632
1.54091
-1.2333
0.8047
0.6753
0.606543
1.570803
0.855367
-0.459018
-0.582853
0.406343
0.719033
-0.194797
-0.28381
-0.833923
-0.531763
0.42202
1.08411
-0.3922
-1.525713
-1.192
-1.0639
-1.187
-1.92991
-1.15125
1.28526
-0.44122
2.80237
0.370074
0.855367
0.431947
-0.64165
0.782727
0.440433
-0.4555
0.480267
-0.379197
-0.395323
0.335997
-0.379333
-0.434397
0.728988
-0.308987
-0.5397
0.606177
-0.979363
1.34859
-1.1141
-1.75735
-0.431027
0.367928
-0.605913
-0.750893
0.34082
0.862043
0.392267
0.452408
0.442176
-1.216293
0.294633
0.78251
0.35311
0.517423
-0.942727
-0.82546 / 0.0087
0.0283
0.0022
0.0004
0.0003
0.0027
0.0026
0.0017
0.0009
0.0036
0.001
0.0052
0.0005
0.0048
0.009242
0.003699
0.003006
0.002894
0.004033
0.0039
0.0087
0.0031
0.001
7.34314E-05
0.78493231
0.0002697
0.0008
0.0027
0.0015
0.0173
0.0016
0.00509075
0.0017
0.007
0.00016049
0.0023
0.0016
0.0099
0.0067
0.0117
0.0111
0.0024
0.0109
0.0047
0.0006
0.0037
0.0023
0.0042
0.0022
0.0058
0.0063
0.008114
0.00524
0.007058
0.0002
0.0018
0.0017
0.0169
0.0206
0.0046
0.0048
0.0058
0.0029
0.0078
0.0046
0.0022
0.0019
0.0023
0.0018
0.0021
0.0019
0.001102
0.005027
0.000387
0.006735
0.003227
0.008595
0.003442
0.007584
5.26E-05
0.0011
0.0015
0.0189
0.0085
0.0027
0.0039
0.0083
0.004
0.0085
0.0038
0.007129
0.004975
0.003258
0.008146
0.005339
0.0067
0.0002
0.0008
0.0053

a log fc expression change (-) up regulated gene expression, (+) down regulated

Gene expression patterns at 1 day after administration of a single dose of hepatitis B vaccine, examined by microarray analysis showed fold change (FC) and P value .(P < 0.05)