Title:
Use of Centrifuge / Rev No: / 2
Issue Date: / 25-Nov-16
Page: / 1 of 2
Prepared by PI Approved Date
Hsu MyatNoe Dr. Chen Chia-Hung 01/12/2016

OBJECTIVES:

This procedure states the proper use of the refrigerated centrifuge in the Integrated Microfluidic Biotechnology Laboratory at the National University of Singapore (NUS).

SCOPE:

This procedure is applicable to all authorised users who carry out research work using the autoclave. All staff and students are to adopt the safety practices in the procedure

PROCEDURE:

3. Wear disposable gloves when there is a risk of contamination.

4. Ensure that the centrifuge is switched on at the wall plug socket.

5. Keep the lid of the centrifuge open when it is not in use

6. Unscrew the lid of the rotator bucket to be used.

7. Place the tubes in the tri union rings inside the rotator buckets.

8. Ensure that the centrifuge is balanced, making sure on opposite sides there is an identical tube balanced by weight.

9. Ensure that the rotator buckets are firmly closed with the covers in place for each centrifugal run.

10 Set the time and speed on the centrifuge according to study protocol and press start.

Title:
Use of Centrifuge / Rev No: / 0
Issue Date: / 25-Nov-2016
Page: / 2 of 2

SAFE OPERATING PROCEDURE

If the refrigeration unit is operating, keep the chamber door closed as much as possible to prevent moisture build up inside the chamber.

If you notice any unusual vibrations, sounds or odours, TURN THE POWER OFF until the cause can be determined.

Turn the POWER switch OFF when centrifuge is not in regular use, to conserve power, and reduce equipment wear and tear.

Keep the rotor and drive hub contact surfaces clean and lightly lubricated with ‘Spinkote’ lubricant, to prevent the rotor sticking to the drive-shaft.

Ensure the rotor is securely installed prior to use.

When removing the rotor, lift it vertically to avoid bending the driveshaft.

Periodically defrost the chamber and wipe up accumulated moisture.

Clean the condenser fins at the back of the instrument every 4-6 months.

Do not operate the instrument if any panels have been removed – this is an electrical shock / mechanical injury hazard.

Do not operate the equipment if the ambient temperature exceeds 38°C.

Do not operate the equipment unless it has a valid electrical testing tag.

Appropriate personal protective equipment like respirators, face shield, goggles and gloves shall be used when required.

Risk assessment must be conducted to decide on the type of PPE required for the work.

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