Supplementary Material for

DynamicalIntrinsic Functional Architecture of the Brain during Absence Seizure

Running Title:functional dynamics during absence seizures

Wei Liao1,2,3†,*, Zhiqiang Zhang3,†, Dante Mantini4,5, Qiang Xu3, Gong-Jun Ji6, Han Zhang1,2, Jue Wang1,2, Zhengge Wang3, Guanghui Chen7, Lei Tian8, Qing Jiao3, Yu-Feng Zang1,2, Guangming Lu3,*

1 Center for Cognition and Brain Disorders and the Affiliated Hospital, Hangzhou Normal University, Hangzhou 310015, China.

2 Zhejiang Key Laboratory for Research in Assessment of Cognitive Impairments, Hangzhou 310015, China.

3Department of Medical Imaging, 7 Department of Neurology, and 8 Department of Neurosurgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.

4 Department of Experimental Psychology, University of Oxford, Oxford OX1, United Kingdom.

5 Department of Health Sciences and Technology, ETH Zurich, Zurich 8057, Switzerland.

6 State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing 100875, China.

†These authors contributed equally to this work.

*Corresponding author: Wei Liao, Center for Cognition and Brain Disorders and the Affiliated Hospital, Hangzhou Normal University, Hangzhou 310015, China. Fax: 86-571-88285651. E-mail: (W. Liao); Guangming Lu, the Department of Medical Imaging, Nanjing Jinling Hospital, 305#, Eastern Zhongshan Rd., Nanjing 210002, China. Fax: 86-25-84804659. E-mail: (G. Lu).

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Table S1

Regions of interest (ROI) in the AAL template

Region name / Abbreviation
Precentral / PreCG
Superior frontal gyrus (dorsolateral) / SFGdor
Superior frontal gyrus (orbital part) / ORBsup
Middle frontal gyrus / MFG
Middle frontal gyrus (orbital part) / ORBmid
Inferior frontal gyrus (opercular part) / IFGoperc
Inferior frontal gyrus (triangular part) / IFGtriang
Inferior frontal gyrus (orbital part) / ORBinf
Rolandic operculum / ROL
Supplementary motor area / SMA
Olfactroy cortex / OLF
Superior frontal gyrus (medial) / SFGmed
Superior frontal gyrus (medial orbital) / ORBsupmed
Rectus gyrus / REC
Insula / INS
Anterior cingulate gyri / ACG
Median cingulate gyri / MCG
Posterior cingulate gyrus / PCG
Hippocampus / HIP
Parahippocampalgyrus / PHG
Amygdala / AMYG
Calcarine fissure / CAL
Cuneus / CUN
Lingual gyrus / LING
Superior occipital gyrus / SOG
Middle occipital gyrus / MOG
Inferior occipital gyrus / IOG
Fusiform gyrus / FFG
Postcentralgyrus / PoCG
Superior parietal gyrus / SPG
Inferior parietal gyrus / IPG
Supramarginalgyrus / SMG
Angular gyrus / ANG
Precuneus / PCUN
Paracentral lobule / PCL
Caudate nucleus / CAU
Putamen / PUT
Pallidum / PAL
Thalamus / THA
Heschlgyrus / HES
Superior temporal gyrus / STG
Superior temporal gyrus, temporal pole / TPOsup
Middle temporal gyrus / MTG
Middle temporal gyrus, temporal pole / TPOmid
Inferior temporal gyrus / ITG

Abbreviations used in the study, differing slightly from the original abbreviations by Tzourio-Mazoyer (Tzourio-Mazoyer et al., 2002).

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Fig. S1.

Figure S1.Dynamic FC maps in AS obtained using 30 volumes (60s) as sliding-window length. Significantly positive (warm colors) and negative (cool colors) FC from thalamus seed (A) and PCC/PCUN seed (B) were derived from second-level random-effects analysis (uncorrected height threshold P<0.05, and extent threshold k=5 voxels). They are presented on inflated surface maps for preictal (Upper), ictal (Middle) and postictal periods (Lower). The dynamic FC maps are shown from -22s to +32s (with steps of 2s), relative to seizure onset. The significantly changed FC values of thalamus (C) and PCC/PCUN (D) for all subjects and all seizures within preictal, ictal, and postictal intervals were assessed using a one-way within-subject ANOVA. The significant FC changes (warm colors) are presented on inflated surface maps (Upper) and axial maps (Lower) (FDR-corrected height threshold P<0.05, and extent threshold k=5 voxels).

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Fig. S2.

Figure S2.Dynamic FC maps in AS obtained using100 volumes (200s) as sliding-window length. Significantly positive (warm colors) and negative (cool colors) FC from thalamus seed (A) and PCC/PCUN seed (B) were derived from second-level random-effects analysis (uncorrected height threshold P<0.05, and extent threshold k=5 voxels). They are presented on inflated surface maps for preictal (Upper), ictal (Middle) and postictal periods (Lower). The dynamic FC maps are shown from -22s to +32s (with steps of 2s), relative to seizure onset. No significantly changed FC was found for all subjects and all seizures within preictal, ictal, and postictal intervals using a one-way within-subject ANOVA.

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