PEER reviewed Publications
1. Baeckström, D., N. Karlsson and G. C. Hansson (1994). "Purification and characterization of sialyl-Le a /carrying mucins of human bile; evidence for the presence of MUC1 and MUC3 apoproteins." J. Biol. Chem. 269: 14430-14437.
2. Karlsson, N. G. and G. C. Hansson (1995). "Analysis of monosaccharide composition of mucin oligosaccharide alditols by high-performance anion-exchange chromatography." Anal. Biochem. 224: 538-541, 35 citations
3. Karlsson, N. G., H. Karlsson and G. C. Hansson (1995). "Strategy for the investigation of O-linked oligosaccharides from mucin based on the separation into neutral, sialic acid- and sulfate-containing species " Glycoconj. J. 12: 69-76, A significant paper for how to increase derivatisation yield in organic derivatisation of biomolecules by a flow devise, 27 citations
4. Karlsson, N. G., M. E. V. Johansson, N. Asker, H. Karlsson, S. J. Gendler, I. Carlstedt and G. C. Hansson (1996). "Molecular characterization of the large heavily glycosylated domain glycopeptide from the rat small intestinal Muc2 mucin." Glycoconj. J. 13: 823-831, 14 citations
5. Karlsson, N. G., H. Karlsson and G. C. Hansson (1996). "Sulphated mucin oligosaccharides from porcine small intestine analysed by four-sector tandem mass spectrometry " J. Mass Spectrom. 31: 560-572, At the time it significantly increased the knowledge about sulfated mucin oligosaccharides, where this paper provided a molecular description of more sulfated mucin oligosaccharides, as had ever been described before, 32 citations
6. Karlsson, N. G., A. Herrmann, H. Karlsson, M. E. V. Johansson, I. Carlstedt and G. C. Hansson (1997). "The glycosylation of the rat small intestinal Muc2 mucin varies between rat strains and the small and large intestine." J. Biol. Chem. 272: 27025-27034, First description of how glycosylation of a defined mucin varies between individual and between tissues, 27 citations
7. Karlsson, N. G., H. Nordman, H. Karlsson, I. Carlstedt and G. C. Hansson (1997). "Glycosylation differences between pig gastric mucin population: a comparative study of the neutral oligosaccharides using mass spectrometry." Biochem. J. 326: 911-917, First time the regional complexity of glycosylation in stomach was described on a molecular level, 22 citations
8. Nordman, H., J. R. Davies, A. Herrmann, N. G. Karlsson, G. C. Hansson and I. Carlstedt (1997). "Mucus glycoproteins from pig gastric mucosa: identification of different mucin populations from the surface epithelium." Biochem. J. 326: 903-910, 26 citations
9. Thomsson, K. A., N. G. Karlsson, H. Karlsson and G. C. Hansson (1997). "Analysis of permethylated glycoprotein oligosaccharide fractions by gas chromatography and gas chromatography-mass spectrometry." Techniques in Glycobiology: 335-347.
10. Thomsson, K. A., I. Carlstedt, N. G. Karlsson, H. Karlsson and G. C. Hansson (1998). "Different O-glycosylation of respiratory mucin glycopeptides from a patient with cystic fibrosis." Glycoconj. J. 15: 823-833, 14 citations
11. Sikut, R., C. X. Andersson, A. Sikut, J. Fernandez-Rodriguez, N. G. Karlsson and G. C. Hansson (1999). "Detection of CD43 (leukosialin) in colon adenoma and adenocarcinoma by novel monoclonal antibodies against its intracellular domain." Int. J. Cancer 82: 52-58, 14 citations
12. Thomsson, K. A., N. G. Karlsson and G. C. Hansson (1999). "Liquid chromatography-electrospray mass spectrometry as a tool for the analysis of sulfated oligosaccharides from mucin glycoproteins." J. Chromatogr. A 854: 131-139, First description of succesful characterisation of sulfated mucin oligosaccharides by LC-MS, 41 citations
13. Ciopraga, J., J. Ångström, J. Bergström, T. Larsson, N. Karlsson, C. Motas, O. Gozia and S. Teneberg (2000). "Isolectins from Solanum tuberosum with different detailed carbohydrate binding specificities: Unexpected reconition of lactosylceramide by N -Acetyllactosamine-binding lectins." J. Biochem. (Tokyo) 128: 855-867.
14. Karlsson, N. G., F. J. Olson, P.-K. Jovall, Y. Andersch, L. Enerbäck and G. C. Hansson (2000). "Identification of transient glycosylation alterations of sialylated mucin oligosaccharides during infection by the rat intestinal parasite Nippostrongylus brasiliensis " Biochem. J. 350: 805-814. First time an active mucosal barrier for glycoprotection against pathogens is demonstrated, 33 citations
15. Axelsson, M. A., N. G. Karlsson, D. M. Steel, J. Ouwendijk, T. Nilsson and G. C. Hansson (2001). "Neutralization of pH in the Golgi apparatus causes redistribution of glycosyltransferases and changes in the O-glycosylation of mucins." Glycobiology 11(8): 633-644. Shared Prime Authorship. First time it was demonstrated that Golgi pH is responsible for the localisation of glycosyltransferases, 32 citations
16. Karlsson, N. G. and N. H. Packer (2002). "Analysis of O-linked oligosaccharides released by an in-line flow system." Anal. Biochem. 305: 173-185, In here is a description of a method to release O-linked oligosaccharides with intact reducing end. This approach is still the most reliable way to release O-linked oligosaccharides for further derivatisation and was granted a patent, 22 citations
17. Olson, F. J., M. E. Johansson, K. Klinga-Levan, D. Bouhours, L. Enerbäck, G. C. Hansson and N. G. Karlsson (2002). "Blood group A glycosyltransferase occurring as Alleles with high sequence difference is transiently induced during a Nippostrongylus brasiliensis parasite infection." J. Biol. Chem. 277(17): 15044-15052, 15 citations
18. Ångström, J., M. Bäckström, A. Berntsson, N. Karlsson, J. Holmgren, K.-A. Karlsson, M. Lebens and S. Teneberg (2000). "Novel carbohydrate binding site recognizing blood group A and B determinants in a hybrid of cholera toxin and Escherichia coli heat-labile enterotoxin B-subunit." J. Biol. Chem. 275: 3231-3238.
19. Packer, N., N. Wilson, B. Schulz, H. Joshi, C. Cooper and N. Karlsson (2002). "Glycomics: Realistic expectations." Glycobiology 12(10): 649-649.
20. Schulz, B. L., N. H. Packer and N. G. Karlsson (2002). "Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis." Anal. Chem. 74(23): 6088-6097, First description of how to perform proteomic O-linked glycosylation analysis in the sub-picomole level, 64 citations
21. Schulz, B. L., N. H. Packer, D. Oxley and N. G. Karlsson (2002). "Identification of two highly sialylated human tear-fluid DMBT1 isoforms: the major high-molecular-mass glycoproteins in human tears " Biochem. J. 366: 511-520, It was demonstrated that the major O-linked glycoprotein in tear fluid was not a classical mucin as was previously believed, and a total analysis of a very complex glycosylation pattern of this protein was provided from as little as 10 µL of human tear fluid. Involved development of novel SDS-PAGE gelelectrophoresis chemistry to allow separation of 10 MDa glycoproteins, 34 citations
22. Wilson, N. L., B. L. Schulz, N. G. Karlsson and N. H. Packer (2002). "Sequential Analysis of N - and O -linked Glycosylation of 2D-PAGE Separated Glycoproteins" J. Proteome Res. 1: 521-529. Number of citations: 49 IF 5.611 Shared Prime Authorship. First comprehensive analysis of the glycosylation of glycoprotein in human plasma, 58 citations
23. Robinson, L. J., N. G. Karlsson, A. S. Weiss and N. H. Packer (2003). "Proteomic analysis of the genetic premature aging disease Hutchinson Gilford progeria syndrome reveals differential protein expression and glycosylation." J. Proteome Res. 2(5): 556-557, 11 citations
24. Holmén, J. M., N. G. Karlsson, L. H. Abdullah, S. H. Randell, J. K. Sheehan, G. C. Hansson and C. W. Davis (2004). "Mucins and their O-Glycans from Human Bronchial Epithelial Cell Cultures." Am. J. Physiol. Lung Cell. Mol. Physiol. 287: L824-L834. Shared Prime Authorship, 48 citations
25. Joshi, H. J., M. J. Harrison, B. L. Schulz, C. A. Cooper, N. H. Packer and N. G. Karlsson (2004). "Development of a mass fingerprinting tool for automated interpretation of oligosaccharide fragmentation data." Proteomics 4(6): 1650-1664, Description of the first web-based tool for MS/MS fragmentation analysis of oligosaccharides. Patented
26. Karlsson, N. G., B. L. Schulz and N. H. Packer (2004). "Structural determination of neutral O-linked oligosaccharide alditols by negative ion LC-electrospray-MS(n) " J. Amer. Soc. Mass Spectrom. 15: 659-672, MS-MS fragmentation rules for oligosaccharides, allowing negative ion MS-MS of oligosaccharides to become a standard tool for characterisation. Including description of retro Diels Alder reactions in ion trap fragmentation for identification of oligosaccharide branching, 28 citations
27. Karlsson, N. G., N. L. Wilson, H. J. Wirth, P. Dawes, H. Joshi and N. H. Packer (2004). "Negative ion graphitised carbon nano-liquid chromatography/mass spectrometry increases sensitivity for glycoprotein oligosaccharide analysis." Rapid Commun.Mass Spectrom. 18(19): 2282-2292, First description of nano-flow LC-MS of oligosaccharides with femtomole sensitivity, 50 citations
28. Schulz, B. L., A. J. Sloane, L. J. Robinson, L. T. Sebastian, A. R. Glanville, Y. Song, A. S. Verkman, J. L. Harry, N. H. Packer and N. G. Karlsson (2004). "Mucin glycosylation changes in cystic fibrosis lung disease are not manifest in submucosal gland secretions." Biochem. J. 387: 911-919, Miniaturized preparation (less than 400 nL) of sub cellular gland secretion from clinical research of human lung ex-plants form CF and normal patient allowed us to show that there is no difference between healthy and diseased patients, contrary to the current dogma at the time, 13 citations
29. Karlsson, N. G., B. L. Schulz, N. H. Packer and J. M. Whitelock (2005). "Use of graphitised carbon negative ion LC-MS to analyse enzymatically digested glycosaminoglycans." J Chromatogr B Analyt.Technol.Biomed.Life Sci 824(1-2): 139-147, 21 citations
30. Thomsson, K. A., B. L. Schulz, N. H. Packer and N. G. Karlsson (2005). "MUC5B Glycosylation in Human Saliva Reflects Blood Group and Secretor Status." Glycobiology 15(30): 791-804, 16 citations
31. Packer, N. H. and N. G. Karlsson (2006). "There are no facts, only interpretations." J. Proteome Res. 5(6): 1291-1292.
32. Estrella, R. P., J. M. Whitelock, N. H. Packer and N. G. Karlsson (2007). "Graphitized Carbon LC-MS Characterization of the Chondroitin Sulfate Oligosaccharides of Aggrecan." Anal. Chem. 79 3597-3606, 12 citations
33. Schulz, B. L., A. J. Sloane, L. J. Robinson, S. S. Prasad, R. A. Lindner, M. Robinson, P. T. Bye, D. W. Nielson, J. L. Harry, N. H. Packer and N. G. Karlsson (2007). "Glycosylation of sputum mucins is altered in cystic fibrosis patients." Glycobiology 17(7): 698-712, Together with my other publications in this field conclusively shows that by employing thorough rigorous high throughput glycoanalysis of high quality the majority of differences between CF and non-CF individual glycosylation as previously reported are due to individual glycosylation differences and by increasing the investigation population the significant differences do not longer exists. This research was patented and a proof of concept device was developed for monitoring of CF-patients lung infection status based on glycobiomarkers identified in infected lungs, 14 citations
34. Wada, Y., P. Azadi, C. E. Costello, A. Dell, R. A. Dwek, H. Geyer, R. Geyer, K. Kakehi, N. G. Karlsson, K. Kato, N. Kawasaki, K. H. Khoo, S. Kim, A. Kondo, E. Lattova, Y. Mechref, E. Miyoshi, K. Nakamura, H. Narimatsu, M. V. Novotny, N. H. Packer, H. Perreault, J. Peter-Katalinic, G. Pohlentz, V. N. Reinhold, P. M. Rudd, A. Suzuki and N. Taniguchi (2007). "Comparison of the Methods for Profiling Glycoprotein Glycans: HUPO HGPI (Human Proteome Organisation Human Disease Glycomics/Proteome Initiative) Multi-institutional Study." Glycobiology 17(4): 411-422, An international consortium comparing different techniques for glycomic analysis. Showed that our method for analysis was provided high quality data in less time and resources compared to other methods utilized in the research community, 89 citations
35. Wilson N. L., L. J. Robinson, A. Donnet, L. Bovetto, N.H. Packer and N. G. Karlsson (2008). "Glycoproteomics of Milk: Differences in sugar epitopes on human and bovine milk fat globule membranes." J. Proteome Res. 9:3687-3696, 6 citations
36. Estrella, R. P, J. M. Whitelock, R.H. Roubin, N. H. Packer and N. G. Karlsson (2009) "Small-scale enzymatic digestion of glycoproteins and proteoglycans for analysis of oligosaccharides by LC-MS and FACE gel electrophoresis." Meth. Mol. Biol. 534:171-192
37. Karlsson N.G. and K. A. Thomsson (2009) "Salivary MUC7 is a Major Carrier of Blood Group I Type O-linked Oligosaccharides Serving as the Scaffold for Sialyl Lewis x." Glycobiology. 19(3): 288-300, 3 citations
38. Olson F. J., R. I. Ludowyke, N. G. Karlsson (2009)"Discovery and identification of serine and threonine phosphorylated proteins in activated mast cells: Implications for regulation of protein synthesis in the rat basophilic leukemia mast cell line RBL-2H3." J. Proteome Res. 8:3068-3077
39. Estrella, R. P., J. M. Whitelock, N. H. Packer and N. G. Karlsson (2010) " The glycosylation of human synovial lubricin: implications for its role in inflammation." Biochem J. 429(2): 359-367. This work was selected as featured article in Nature CFG functional glycomics gateway http://www.functionalglycomics.org/fg/update/2010/100708/full/fg.2010.24.shtml describing a mucin molecule as a biomarker candidate for osteoarthritis and rheumatoid arthritis.
40. Issa, S., A. P. Moran, S. U. Ustinov, j. H.-H. Lin, A. J. Ligtenberg and N. G. Karlsson (2010) "O-linked oligosaccharides from salivary agglutinin: Helicobacter pylori binding sialyl-Lewis x and Lewis b are terminating moieties on hyperfucosylated oligo-N-acetyllactosamine" Glycobiology 20(8): 1046-1057
41. Jensen, P. H., N. G. Karlsson, D. Kolarich and N. H. Packer (2010) "Same but different: separation of released N- and O-glycans" Methods Mol Biol. accepted
42. Wada, Y., A. Dell, S. M. Haslam, B. Tissot, K. Canis, P. Azadi, M. Bäckström, C. E. Costello, G. C. Hansson, Y. Hiki, M. Ishihara, H. Ito, K. Kakehi, N. Karlsson, K. Kato, N. Kawasaki, K. H. Khoo, K. Kobayashi, D. Kolarich, A. Kondo, C. Lebrilla, M. Nakano, H. Narimatsu, J. Novak, M. V. Novotny, E. Ohno, N. H. Packer, M. B. Renfrow, M. Tajiri, K. A. Thomsson, H. Yagi, S. Y. Yu and N. Taniguchi (2010) " Comparison of Methods for Profiling O-glycosylation: HUPO Human Disease Glycomics/Proteome Initiative Multi-Institutional Study of IgA1." Mol. Cell. Proteomics 9(4): 719-727, An international consortium comparing different techniques for glycomic analysis. Showed that our method for analysis was provided high quality data in less time and resources compared to other methods utilized in the research community.
43. Hayes, C. A., R. Doohan, D. Kirkley, B. Harhen, , A. V. Savage and N. G. Karlsson (2011) "Cross validation of Liquid Chromatography-Mass Spectrometry and Lectin Array for Monitoring Glycosylation in Fed-Batch Glycoprotein Production" J. Biotech. in print
44. Hayes, C. A., S. Nemes, S. Issa, C. Jin and N. G. Karlsson (2011) "Glycomic Work Flow for Analysis of Mucin O-linked oligosaccharides." Methods Mol Biol. accepted
45. Doohan, R. A., C. A. Hayes, B. Harhen and N. G. Karlsson (2011) " Negative ion CID Fragmentation of O-linked Oligosaccharide Aldoses- Charge Induced and Charge Remote Fragmentation." J. Am. Soc. Mass Spectrom. accepted