Name______Date______Per._____
Light Microscopy Investigation
Today you will:
· Observe preserved slides of plant & animal tissue
· Be able to identify cellular structures (membrane, nucleus, cell wall etc.)
Pre-lab
1. Orient yourself with your microscope; match the labels on the right to the diagram on the left:
1.
2. Read “Rules for Scientific Drawings” (see the last page of this lab). What are 5 things you need to include in a good scientific sketch?
3. How do you calculate the total magnification if you are using the highest power on your microscope?
Lab
Directions: Obtain four slides, you must include at least one plant slide of the specimens you choose to sketch. Be sure to follow the “rules for scientific drawings” & shade with color when applicable. Label ALL structures you see, if you are unsure of what to label….ask. You may want to do some background research on the specimens.
Specimen 1
Specimen 2
Specimen 3
Specimen 4
Post Lab Questions
1. The study of cell structure has made significant progress due to improvements in microscopy that enhance magnification, resolution, and contrast. What are the different types of microscopes? Describe what they are best used for (pg. 96 of Campbell)
2. What are three cellular components that are visible when using fluorescence microscopy?
3. Of the 4 tissues you observed, how does their structure relate to their function? (i.e. how do these cells look, and how does it relate to the function of the entire system) Be specific!
RULES FOR SCIENTIFIC DRAWINGS
Scientific drawings can be made using several methods, depending on a particular laboratory investigation. For a microscopy lab, the drawings are made in circles that represent the viewing field of a microscope. For other labs such as dissection labs, the drawings are representative of the entire organism or parts of the organism. These drawings show the relative size, shape, and location of anatomical structures. Most scientific drawings are labeled.
Use the following guidelines to help make your scientific drawing as clear as possible:
· Use pencil (& color when applicable)
· Title your drawing
· Use a ruler to draw label lines
· Do not use arrows for label lines
· Label lines should point to the center of the structure being labeled
· Print all labels horizontally
· Print, do not write in cursive
· Label the right-hand side of the drawing, if possible
· Do not cross label lines
· Include magnification (if a microscopic drawing) or a scale (if a macroscopic drawing)
Total magnification = ocular magnification multiplied by the objective magnification
MOST light microscopes:
Ocular magnification; 10x
Scanning 4x
Low power 10x
High power 40x
MAKE YOUR DRAWING AS BIG AS POSSIBLE WITHIN YOUR FIELD OF VIEW!!!
(No drawings on the scanning objective!)