LOCUS pMA2008-HA 4110 bp DNA circular 31-JAN-2006

DEFINITION CMV-Km + HA +1 Reading frame.

SOURCE

ORGANISM

COMMENT This file is created by Vector NTI

http://www.invitrogen.com/

COMMENT VNTDATE|393878263|

COMMENT VNTDBDATE|400878804|

COMMENT LSOWNER|

COMMENT VNTNAME|pMA2008-HA|

COMMENT VNTAUTHORNAME|Mikhail Alexeyev|

FEATURES Location/Qualifiers

CDS 2006..2800

/codon_start=1

/transl_table=11

/product="neomycin phosphotransferase"

/protein_id="AAB08059.1"

/db_xref="GI:1373317"

/vntifkey="4"

/label=neoR

promoter 1..589

/vntifkey="29"

/label=Pcmv-human

rep_origin 3385..4028

/vntifkey="33"

/label=ColE1ori

promoter 1655..1884

/vntifkey="29"

/label=Psv40

rep_origin 1822..1957

/vntifkey="33"

/label=SV40ori

polyA_signal 2975..3245

/vntifkey="25"

/label=HSVTk\polyA

promoter 1543..1577

/vntifkey="30"

/label=Km-prom

polyA_signal 787..1015

/vntifkey="25"

/label=SV40\polyA

rep_origin 1026..1481

/vntifkey="33"

/label=f1\ori

CDS 611..643

/vntifkey="4"

/label=HAtag\hum

BASE COUNT 985 a 1077 c 1069 g 979 t

ORIGIN

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagatcgc

601 caccatggct tacccctacg acgtgcccga ctacgccagc ctgggaattc ctgcagcccg

661 ggggatccac tagttctaga gcggccgcca ccgcggtgga gctcgaattg acgcgtcctc

721 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatgatcc accggatcta

781 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc

841 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt

901 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag

961 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg

1021 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag

1081 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac

1141 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga

1201 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc

1261 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg

1321 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa

1381 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac

1441 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg

1501 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct

1561 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc

1621 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca

1681 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc

1741 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata

1801 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg

1861 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag

1921 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca

1981 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc

2041 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc

2101 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga

2161 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac

2221 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct

2281 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa

2341 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc

2401 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct

2461 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc

2521 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg

2581 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct

2641 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct

2701 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca

2761 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa

2821 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc

2881 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc

2941 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag

3001 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac

3061 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat

3121 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac

3181 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc

3241 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa

3301 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt

3361 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt

3421 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt

3481 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag

3541 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta

3601 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat

3661 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg

3721 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg

3781 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac

3841 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga

3901 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt

3961 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta

4021 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat

4081 tctgtggata accgtattac cgccatgcat

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