Supplementary Table S2. Expression of miR-199b-5p and its target genes, as determined by Taqman PCR assay and Syber qRT-PCR analyses, respectively.

Cell type / Detector / SNALP miR-199b-5p / SNALP scramble
Mean 2-DCt / SD 2-DCt / Mean 2-DCt / SD 2-DCt
miR-199b-5p expression (24 h); Taqman assaya
Caco-2 / miR-199b-5p / 8.105396954 / 1.161515914 / 1.147002948 / 0.392869356
MCF7 / miR-199b-5p / 11.33014485 / 2.271964596 / 0.212975471 / 0.017386405
ONS76 / miR-199b-5p / 6.047608675 / 1.332294101 / 0.778918058 / 0.210582011
PC3 / miR-199b-5p / 1.597711451 / 0.287265371 / 0.264436496 / 0.012405414
SW480 / miR-199b-5p / 5.83567391 / 1.818648075 / 3.076789812 / 0.630019649
U87 / miR-199b-5p / 0.082608936 / 0.013935299 / 1.614055877 / 0.607327219
miR-199b-5p expression (72 h); Taqman assaya
Caco-2 / miR-199b-5p / 2.54691E-07 / 6.10192E-09 / 9.83871E-10 / 5.34932E-11
MCF7 / miR-199b-5p / 1.75666E-07 / 2.3828E-08 / 6.21092E-10 / 6.81144E-11
ONS76 / miR-199b-5p / 4.19016E-07 / 3.93322E-08 / 4.71908E-10 / 3.26541E-12
PC3 / miR-199b-5p / 7.48397E-07 / 3.05608E-08 / 1.99553E-09 / 5.0128E-10
SW480 / miR-199b-5p / 1.62973E-09 / 1.49317E-10 / 1.88123E-07 / 3.62646E-09
U87 / miR-199b-5p / 0.015766 / 0.00177 / 0.00101 / 0.0075
HT29 / miR-199b-5p / 1.71463E-07 / 1.21835E-07 / 7.90228E-12 / 2.12281E-11
Daoy / miR-199b-5p / 0.007856317 / 0.026380381 / 0.000425433 / 0.013737259
MDA 231T / miR-199b-5p / 1.107312693 / 0.023224805 / 0.003150241 / 5.49552E-05
UW228 / miR-199b-5p / 0.196255112 / 0.026188556 / 0.020188416 / 0.001545945
Target expression (72 h); Syber assayb
MCF7 / Hes1 / 0,01205637 / 0,000459877 / 0.02301429 / 0.002758875
ONS76 / Hes1 / 0.010585962 / 0.000379695 / 0.009340655 / 0.000178252
SW480 / Hes1 / 0.005153932 / 0.000174103 / 0.003464579 / 0.000701527
U87 / Hes1 / 0.000216374 / 1.1825E-05 / 0.000170995 / 3.11536E-05
HT29 / Hes1 / 0.000880279 / 0.019579563 / 2.87979E-06 / 0.008782629
Daoy / Hes1 / 0.001634064 / 0.00355954 / 0.000313473 / 0.004929662
MDA 231T / Hes1 / 0.003020547 / 0.001874891 / 0.009672218 / 0.002788663
UW228 / Hes1 / 0.007008677 / 0.002725455 / 0.005758379 / 0.004070443
PC-3 1 / Hes1 / 2.3766E-05 / 7.32035E-06 / 0.274980901 / 0.004852498
CaCo-2 / Hes1 / 0.00011096 / 1.50264E-05 / 0,00181357 / 0,000398916
ONS76 / DDR1 / 0.012045794 / 4.41441E-05 / 0.014575246 / 0.000379876
SW480 / DDR1 / 0.001274933 / 0.000268909 / 0.001405448 / 7.04344E-05
U87 / DDR1 / 0.00087592 / 2.27322E-05 / 0.001068259 / 0.000112435
ONS76 / HIF1A / 0.058197648 / 0.002175474 / 0.054917802 / 0.001090409
SW480 / HIF1A / 0.111414488 / 0.011858809 / 0.081724835 / 0.000300137
U87 / HIF1A / 0.017591182 / 8.1641E-05 / 0.01713092 / 0.000119026
ONS76 / PODXL1 / 0.019779834 / 0.000480789 / 0.022579604 / 0.000535611
SW480 / PODXL1 / 0.001679396 / 0.000129057 / 0.001985203 / 0.000134768
U87 / PODXL1 / 0.000501553 / 5.0285E-05 / 0.000551996 / 4.35761E-05

aTaqman PCR values of miR-199b-5p expression at 24 h and 72 h of treatment with SNALP miR-199b-5p and SNALP scramble in the different cell lines.

bSyber qRT-PCR values of Hes1, DDR1, Hif-1a, and PODXL-1 mRNA expression at 72 h of treatment with SNALP miR-199b-5p and SNALP scramble in the different cell lines.

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