Supplementary figure legends, figures and tables
S-Fig. 1 Profile of 1kb DNA Marker #SM0331 (Fermentas, USA) (lane 1) and the dsRNA extracted from the mycelia (lane 2) in 1% agarose gel electrophoresis.
S-Fig. 2 The electrophoretic profile of total dsRNAs from virions in 1.5% agarose gel (lane 1) and the corresponding Northern hybridization results (lane 2, 3 and 4). The [32P]dCTP-labeling probes were prepared from the cDNAs of the corresponding dsRNA segments, and the total dsRNAs were transferred onto Hybond-N+ membrane in parallel and was used as the targets.
S-Fig. 1
S-Fig. 2
Supplementary-Table 1 Comparison of the 5′ and 3′ terminal sequences of AoR1 and AoR2 with those contained in Partitiviruses, such as P. stoloniferum virus S (PsV-S), G. abietina RNA virus MS1 (GaRV-MS1), G. abietina RNA virus MS2 (GaRV-MS2), Discula destructiva virus 1 (DdV1) and D. destructiva virus 2 (DdV2). Identical nucleotides with those of AoR1 and AoR2 were marked in bold and with grayish background color.
AoR1[EU118277] / 5′ / CGCAAAACU / UAAAUAACUCC / 3′
AoR2[EU118278] / 5′ / CGCAAAACU / GUCAUAUCUCC / 3′
PsV-S S1 [AM040148] / 5′ / CGCAAAAUC / AAUAAAUCUCU / 3′
PsV-S S2 [AM040149] / 5′ / CGCAAAAUU / AGUAAAUCUCU / 3′
GaRV-MS1 dsRNA1[AY089993] / 5′ / CGCAAAAUA / AUUAACAAUCA / 3′
GaRV-MS1 dsRNA2 [AY089994] / 5′ / CGCAAAAUU / AACCAACCUCU / 3′
GaRV-MS2 dsRNA1[AY615211] / 5′ / CGCAAAAUA / AUUAACAAUCA / 3′
GaRV-MS2 dsRNA2 [AY615212] / 5′ / CGCAAAAUU / AACCAACCUCU / 3′
DdV1 dsRNA1[AF316992] / 5′ / GCGCAAAAG / GUAAAAACUCC / 3′
DdV1 dsRNA2 [AF316993] / 5′ / CGCAAAAUU / CAUAAACCUCC / 3′
DdV2 dsRNA1 [AY033436] / 5′ / GCGCAAAAC / AGUAAAACUCA / 3′
DdV2 dsRNA2 [AY033437] / 5′ / GCGCAAAAG / CAUAAAACUCC / 3′
Supplementary-Table 2 The identity among AoR2 putative CP and those of genus Partitivirus, PsV-S, GaRV-MS1, GaRV-MS2, Botryotinia fuckeliana partitivirus 1 (BfPV1) (unpublished), DdV2, DdV1, Ophiostoma partitivirus 1 (OPV1).
PsV-S [YP_052857] / 63%
GaRV-MS1 [NP_659028] / 56%
GaRV-MS2 [YP_138541] / 56%
BfPV1 [CAM33267] / 58%
DdV2 [NP_620302] / 57%
DdV1 [NP_116742] / 50%
OPV1 [CAJ31887] / 50%