Jre Mamtfgkvva-Dlsqgetwc Gehtvattaniggagtkratraillcasvtccga 53

MAG MTSTFGKIDAFDDSQGETWEHYTERLGQYFVANGIGNEEPRDKEKRRAILLS------V 53

PYG1 MTSTFGKIDAFDDSQGETWEHYTERLGQYFVANGIGNEEPGDKEKRRAILLS------V 53

JRE MAMTFGKVVA-DLSQGETWC------GEHTVATTANIGGAGTKRATRAILLCASVTCCGA 53

JULE MAMHVGRVVQYDESK-EYFESYLERLEQWMLANDVP------DEKVVCTFLS------V 46

*: .*:: * *: * : : :*. .. . :*. .

MAG CGAKVYK---LMS------DLSAPTKP------SEKTY---EDLVV 81

PYG1 CGAKVYK---LMS------DLSAPTKP------SEKTY---EDLVV 81

JRE TGCKVYKCTCLMSCGATTGCCATCAGCETACCGDLSATCTPTKPACGSEKTYTTCEDLVV 113

JULE IGADTYG---LLK------NLVSPKVP------STMEY---AALTA 74

*...* *:. :* :. * * * *..

MAG L------VKEHSA---PKP------SEIVQR--YKFHNRFRKTGESVSQYIAALRNLSE 123

PYG1 L------VKEHSA---PKP------SEIVQR--YKFHNRFRKTGESVSQFIAALRNLSE 123

JRE TGGTVKTGCTEHSATARPKPSACEIVTAATQRTAYKFHNRFRKTGESVSQFIAALRNLSE 173

JULE A------LQAHYK---LTP------VVIAER--FRFQKRNQKEEEPVSDYVVALRQLSA 116

* .* .:* ::*::* :* *.**:::.***:**

MAG HCNFGGTLETMIRDRLVCGINDDRIQRRLLSERELTFTKSYEIAISMETAAKNVIDLQQD 183

PYG1 HCNFGGTLETMIRDRLVCGINEDRIQRRLLSERELTFTKSYEIAISMETAAKNVIDLQQD 183

JRE HCNFT------TTTGCTGTLEACTMIRDRACTLVCG------203

JULE TCNFGQYLDEALRDRFVSGLHSDAAQRKLLAEKDLTFSHACEIAVNMELASKNTMEFSGH 176

*** . * :: :: *: .

MAG TTPAGAEKIHKVSQPKR---GQQRPKSQQQTQGHRAQAPENQGN-----LQKPCFRCGGK 235

PYG1 TTPAGAGKIHKVSQPKR---GQQRPKSQQQTQGHRAQAPDNQGN-----LQKPCFRCGGK 235

JRE --TAGINEAATTDRTCG---IQQRPKSQQQTQGHR--APDNQGN-----LQKPCFRCGGK 251

JULE HDPHQVNALTNVPMFSKRGWKGQHSKEGQNSKEEKHQIKSWMGSKKDTATRQPCYRCGGK 236

. .. *:.*. *::: .: . *. ::**:*****

MAG HKPQNCKFKDSECFYCRKKGHIIEKCLTRLAECKGGRV----THSSD---RKK--QNK-- 284

PYG1 HKPQNCKFKDSECFYCRKKGHIIEKCLTRLAECKGGRV----THSSD---RKK--QNK-- 284

JRE HKPQNCKFKDSECFYCRKKGHIIEKCLTRLAECKGGRVGACCTHSSDCTGRKKTCQNKTT 311

JULE HSARDCRFRNEQCHVCAKIGHIARACRNKKSRQQTQYVETD------277

*..::*:*::.:*. * * *** . * .: :. : *

MAG VNAL----EEVDD------DDIYP----MFRVS--SSNK---EPYTVTLSV------NGI 319

PYG1 VNAL----EEVDD------DDIYP----MFRVS--SSNK---EPYTVTLSV------NGI 319

JRE VNALGAEGREVDDATCCGTDDIYPCCTCMFRVSTASSNKTGAEPYTVAATLSVGTATNGI 371

JULE IEAS----NRNDEAELFG------VYSVYSVASSE---KGYTLDVLLG------312

::* .. *: :: * :*.: : **: :

MAG PLQ--MEIDTGASKTVVG----EP--AFERLTKCDKVT----LKP------SNTKLKTY 360

PYG1 PLQ--MEIDTGASKTVVG----EP--AFERLTKCDKVT----LKP------SNTKLKTY 360

JRE PLQCGMEIDTGASKTVVCTTCGEPCTAFERKCTTDKVTTCETLKPTCTCSNGAGKKPTTY 431

JULE DKNTTMLLDTGSAVSVIS------DKYYQSHLTQFP--IKP------VGLKLKSY 353

: * :***:: :*: :: . . :** . * .:*

MAG TG---EMVPVLGR--FEVPVNYG---KFDGVLQAEVVKGDNPCLLGRDWLWHIQLDWSEI 412

PYG1 TG---EMVPVLGR--FEVPVNYG---KFDGVLQAEVVKGDNPCLLGRDWLQHIQLDWNEI 412

JRE TGTAEMVAPVLGRCGFEVPVNYGAACKFDVLQAVTAAVKDNPCLLGRDWLCQHILDWNFA 491

JULE SG---QSIAVRGC--IVVPVQYE---TQKSILPLIIVQGNRPSLIGRNWLKKLQLNWKQI 405

:* .* * : ***:* . . : . :.*.*:**:** : *:*.

MAG FS----ISHDSDNSIIDEIVSKHPSVFEPGLGKVRGTKAKIYVDPQEKLLYFKARPTPYA 468

PYG1 FS----ISHDSDNSIIDEIVSKHPSVFEPGLGKVRGTKAKIYVDPQEKPLYFKARPTPYA 468

JRE GS----AISAGHDDIIDE--SKHPSVFEPGLGKVRGTKAKIYVDPQEKPLYFKARPT--A 543

JULE FTVHKVLVQKDAKPGLQKVILRHQAVFSDDQGCIKGFKARIRIKPNTAPVFCKARPVPYA 465

: . . ::: :* :**. . * ::* **:* :.*: :: ****. *

MAG LRDKIEHELDRLVAEGTISPVEFSEWATPIVPIVKSDKSIRICGDYKVTVNKVSKLDNYP 528

PYG1 LRDKIEHELDRLVAEGTISPVEFSEWATPIVPIVKSDKSIRICGDYKVTVNKVSKLDNYP 528

JRE LR---EHELDTGCCAAAISP------FSAIVTGTTSDK--RIG---KVTVSKL------582

JULE LKEAVEKELDRLEKMKVISKVEKSEWASPIVTVPKTDKTIRICGDYKVSINQCIEEKHYP 525

*: *:*** .** :.**. .:** ** **::.:

MAG IPKTEDLYATLGGGTDYSKLDLSQAYQQLELDDSSKPYTTINTHKGLFVYNRLPYGVASA 588

PYG1 IPKTEVLYATLGGGTDYSKLDLSQAYQQLELDDSLKPYTTINTHKGLFVYNRLPYGVASA 588

JRE IPK------VLATGSKLS------AQELDDS----KTTITHKGYRLY------VASP 617

JULE LPNAEDLFATLEGGTSFSKLDLSHAYQQLELDEESEKYLVINTHKGLYKYSRLSYGVSSA 585

:*: .* *:. * ***:. . **** * *:*.

MAG PGIFQRTMENLLQGIPQVIVRVDDILVTGKNRRQHLENLEAVLNRLEKAGVKLKRSKCYF 648

PYG1 PGIFQRTMENLLQGIPQVIVRVDDILVTGKNRRQHLENLEAVLNRLDKAGVKLKRSKCYF 648

JRE G--FQR---TLLQGP-----QVVDDLVTG--RRQ----LNEVLNRLDKAGVKLKKDKCEF 661

JULE PAIFQSVMDQILQGMDHVTCFLDDILITASSEKXHLQRLEEVLTRLEKHGLRVKLAKCRF 645

** :*** : * *:*. .: *: **.**:* *:::* ** *

MAG LRKEVEYQGHRINSEGMQPIEGKVMAIKDAPAPTNVKELQAFLGMLNYYSCYLPRLSTTL 708

PYG1 LRKEVEYLGHRINSEGMQPIEGKVMAIKDAPAPTNVKELQALLGMLNYYSCYLPRLSTTL 708

JRE MEKEVIFLGHKVDATGIHPVPEKVQAVQNAPRPTSVTELKAYLGLLNFYNRFLPNLSTLL 721

JULE LQSSVEYLGHRIDKDGLHPMEEKVAAITKAPEPTNVTELKSFLGLLNYYSRFLNNPSTIL 705

:...* : **::: *::*: ** *: .** **.*.**:: **:**:*. :* . ** *

MAG APLYALLGKDVKWKWHKAEDEAFCEAKDMLQSDTLLVHYDPDKELVLSCDASPYGLGALL 768

PYG1 APLYALLGKDVEWKWDKAEDEAFREAKDMLQSDTLLVHYDPDKELVLSCDASPYGLGAVL 768

JRE APLHQLLKKEVPWCWKEEQEEVFKKSKELLQSNWVLIHYDERKELVLSCDASAYGVGAVL 781

JULE QPLHNLLRKDETWIWTTACTQAFEDAKRLLLQNKVLVHYSTRLPLKLACDASPYGVGAVI 765

**: ** *: * * :.* .:* :* .: :*:**. * *:****.**:**::

MAG SHVMLDGRERPIAYASRTLAPAKKHYSQLDKEGAALIFGVRKFHQYLFGQKFVVYTDHKP 828

PYG1 SHVMLDGRERPIAYVSRTLAPAEKNYSQLDKEGAALILGVRKFHQYLFGQKFVVYTDHKP 828

JRE AHRMADGTERPIGFVSRTLTVAEKNYSQLEKEGLAVVFGVKKFHKYLYGRKFVICTDHKP 841

JULE SHILENGEERPVAFASRTLTDAERKYAQIEKEALAIIFGVKKFHKYLYGRKFTLVTDHKP 825

:* : :* ***:.:.****: *:::*:*::**. *:::**:***:**:*:**.: *****

MAG LLGLFKADRAIPSMASARIQRWALLLATYEFDLRYRPGTKNSNADGLSRLPLSETVVNIP 888

PYG1 LLGLFKADRAIPSMASARIQRWGLLLATYEYDLRYRPGTKNSNADGLSRLALSETVVNIP 888

JRE LLTLLNELKAVPQMVSQRIMRWALMLGAYEYVISYRAGKDNGNADALSRLPVPETPE-KE 900

JULE LLTILGPKSAVPTLAALRMQRWALILMAYNYDIEYRRSADHANVDALSRLPWNSPDN--T 883

** :: *:* :.: *: **.*:* :*:: : ** . .:.*.*.****. ..

MAG LPGETLLLLEHLHTTPVTFKHIQEWTAKDPVLIQVLANVQDGWNKCGSEET-KPYFNRKA 947

PYG1 LPGETLLLLEHLDTTPVTFMQIQEWTAKDPVLIQVLAHVQDGWNKCGSEET-KPYFNRKA 947

JRE AKEDYVLMFDSIISPLTTSEQIKHWTTRDLVLSRVREYVLKGWPNCVQFETSKPYFIRSD 960

JULE AEEGSIFYFSHVEELPILAKDIEKATMKDPLLSRVWSYTMNGWPTYVQDETLKPYFIRRQ 943

:: :. : .*:. * :* :* :* . .** . . ** **** *

MAG ELSCHEGVLLHGNRVVVPTPGRAKLLEFLHEGHPGIVRMKALARSHVWWPGIDSDIQDKV 1007

PYG1 ELSCHEDVLLHGNRVVVPKPGRAKLLEFLHEGHPGIVRMKALARSHVWWPGIDSDIQDKV 1007

JRE ELSAEQDFVLWGARIIIPEQGRAGLLEQLHQSHPGMSRMKGLARSYLWWPKLDADIEARV 1020

JULE ELSAEQGCVLWGQRVIIPPSYRQKVLKDLHHEHPGICRMKALARSYLWWPGCDGDIQELV 1003

***..:. :* * *:::* * :*: **. ***: ***.****::*** *.**: *

MAG QACTECQLQIPVPPVAPLHSWDWPDRPWSRIHVDYAGPFLGQMFLVVVDSYSKWLEVVPT 1067

PYG1 QACTECQLQRPVPPVAPLHSWDWPDRPWSRIHVDYAGPFLGQMFLVVVDSYSKWLEVVPT 1067

JRE TNCTVCQEQRKAPVGAPLHLWEWPRQPWRRVHMDYAGPFLGKMFLILVDAHSKWIEAYPI 1080

JULE QSCQICQAVQKLPAVAPLHPWKWPDRAWQRIHIDFAEKDK-HYFLVVIGSHSKWLEVFPM 1062

* ** * **** *.** :.* *:*:*:* : **:::.::***:*. *

MAG NSSTSTVTIAKLRQIFAEHGLPDKLVSDNGPCFISEEFEIFLRENGIQHVKISPHHPATN 1127

PYG1 NSSTSTVTIAKLRQIFAEHGLPDKLVSDNGPCFISEEFETFLRENGIQHVKISPHHPATN 1127

JRE NSATTATTLEYLRKSFSTHGIPEMMVSDNAQCFVSEASKEFMSRNGITHVTSAPYHPSSN 1140

JULE TSITSHNTIEILRRLFSSYGLPEELVSDNGPQLVSQEFCQFLELNGIRHTAVPAYHPASN 1122

.* *: *: **: *: :*:*: :****. ::*: *: *** *. ..:**::*

MAG GLAERSVRIFK-EGMKKMGTSGG------DTAAKLSRFLLSY 1162

PYG1 GLAERSVRIFK-EGMKKMGTSGG------DTAAKLSRFLLSY 1162

JRE GLAERAVQTFK-SLMKKSVTFGASDFGDAGDDFTCAAPKDLSFRDFADLALFASAFYCRA 1199

JULE GAAERSVQILKRSLMKNVLETDG------KATLPLSHRLANFLIMY 1162

* ***:*: :* . **: .. : : *

MAG RSTPQTTTGLSPAELLFNRKLRTKLDLVKP----DVRERVVKRQQTQKNYHDSRAKERVF 1218

PYG1 RSTPQTTTGLSPAELLFNRKLRTKLDLVKP----DVRERVVKRQQTQKNYHDSRAKERVF 1218

JRE STFPPQTTGLSPAELKNDFVAMWKLDLAVDKFPDDVRERVCERQMFQKDEHDFKDCVRVF 1259

JULE RSTPHTVTGCTPAELFLKRQIRTRFSLLKP----ELAQHIKQGQFKQKQHHDESSILRVF 1218

: * .** :**** . ::.* :: ::: : * **: ** ***

MAG AEGDKVFVKNFYSG-PKWKSGEIVSKNSPASYTVEGQNGVRARRHVDHIRQRHCLAVAEP 1277

PYG1 AEGDQVFVKNFYSG-PKWKSGEIVSKTSPVSYTVEGQNGVRARRHVDHIRQRHCLAVAEP 1277

JRE CEGEQFFVKNFYSG-PKWKSGEIVSKTSPVSYTVEGQNGVRARRHVDHADFEAPINPEAP 1318

JULE SEGETVRVRNFRGGFVKWTCGTVLKKLGSVTYRIQDGQRTRTT-HVDHMLPWKQDVEKSP 1277

.**: . *:** .* **..* ::.* ...:* ::. : .*: **** *

MAG NIHQEIFESDAHP------VITAVSDVVTNSKDSSPSVGEKEGSSPKQTKQS 1323

PYG1 NIHQEIFESDAHP------VITAVSDVVTNSKDSSPSVGEKEGSSPKQTKQS 1323

JRE DNFAKFIESDAVPTSFKSPMYGPMFLILVDKDSHIVFSDDTNSSSTAPPFGSSPKQTKQS 1378

JULE VMSSQVLVEEPEEIRDCVVPG-----SLRTGLTASPLPAESPGQTTEEPSVSRPKTTQSP 1332

:.: .:. : : . . :. * ** *:..

MAG SVVP--VVRPQRVKTTPAYLKDYVTQK- 1348

PYG1 SVVP--VVGPQRVKTTPAYLKDYVTQK- 1348

JRE MFGKEDSAIPQRPDSPDKFEEPL----- 1401

JULE SAEG---RYPVRHRVPPKETESLKMYKV 1357

* * . :

Fig,S1 Alignment of protein sequences of JREand Juleelements. Conserved motifs and domains are overlined. Identicalresidues are marked by *.

Allsequence used in allingment include:Mag, Azumapecten farreri, ABM90392.1; PYG1,Mizuhopecten yessoensis, ABM90393.1 JULE, Xiphophorus maculatus ,AF278691.1