Supplementary Table S2:Details of 84 mutations observed among 29,792 (1730 to 1764 per locus) meiotic transfers from analyzing the 17 Y-STR loci included in the AmpFlSTR® Yfiler® PCR amplification kit in DNA-confirmed father-son pairs

Y-STR
Locus / Allele
Fatherg / Allele
Song / Repetitive sequence
with mutated repeat highlighted in reda / Repeat
change / Father’s age f
DYS19 / 17 / 16 / (TATC)14 to 13tacc(TATC)3 / -1 / 37
DYS19 / 17 / 18 / (TATC)14 to 15tacc(TATC)3 / +1 / 31
DYS19 / 17 / 16 / (TATC)14 to 13tacc(TATC)3 / -1 / 29
DYS19 / 15 / 16 / (TATC)12 to 13tacc(TATC)3 / +1 / No data
DYS19 / 16 / 17 / (TATC)13 to 14tacc(TATC)3 / +1 / 46
DYS19 / 17 / 16 / (TATC)14 to 13tacc(TATC)3 / -1 / 24
DYS19 / 14 / 15 / (TATC)11 to 12tacc(TATC)3 / +1 / 43
DYS385a / 13 / 14 / (aagg)6(GAAA)13 to 14 / +1 / 30
DYS385a / 14 / 15 / (aagg)6(GAAA)14 to 15 / +1 / 60
DYS385a / 13 / 12 / (aagg)6 to 5(GAAA)13 / -1 / 22
DYS385b / 14 / 15 / (aagg)6(GAAA)14 to 15 / +1 / 20
DYS385b / 14 / 15 / (aagg)6(GAAA)14 to 15 / +1 / 21
DYS385b / 14 / 15 / (aagg)6(GAAA)14 to 15 / +1 / 23
DYS385b / 11 / 12 / (aagg)6(GAAA)11 to 12 / +1 / 52
DYS385b / 15 / 16 / (aagg)6(GAAA)15 to 16 / +1 / 39
DYS385b / 15 / 16 / (aagg)6(GAAA)15 to 16 / +1 / 48
DYS389I / 14 / 13 / (TAGA)11 to 10(CAGA)3 / -1 / 24
DYS389I / 12 / 13 / (TAGA)9 to 10(CAGA)3 / +1 / 23
DYS389I / 13 / 14 / (TAGA)10 to 11(CAGA)3 / +1 / No data
DYS389I / 15 / 14 / (TAGA)12 to 11(CAGA)3 / -1 / 32
DYS389I / 13 / 12 / (TAGA)10 to 9(CAGA)3 / -1 / 39
DYS389I / 13 / 14 / (TAGA)10 to 11(CAGA)3 / +1 / 46
DYS389I / 14 / 13 / (TAGA)11 to 10(CAGA)3 / -1 / 33
DYS389I / 13 / 14 / (TAGA)10 to 11(CAGA)3 / +1 / 23
DYS389I / 14 / 13 / (TAGA)11 to 10(CAGA)3 / -1 / 26
DYS389II / 18 / 17 / (TAGA)13 to 12(CAGA)5(ca)4 / -1 / 27
DYS389II / 17 / 18 / (TAGA)12 to 13(CAGA)5(ca)4 / +1 / No data
DYS389II / 17 / 18 / (TAGA)12 to 13(CAGA)5(ca)4 / +1 / 24
DYS389II / 18 / 17 / (TAGA)13 to 12(CAGA)5(ca)4 / -1 / 54
DYS389IIb / 17 / 16 / (TAGA)12 to 11(CAGA)5(ca)4 / -1 / 32
DYS389II / 19 / 18 / (TAGA)14 to 13(CAGA)5(ca)4 / -1 / 36
DYS390 / 25 / 24 / (taga)2tga(TAGA)4(CAGA)(TAGA)12-11(CAGA)8(taga)2 / -1 / 32
DYS390 / 25 / 24 / (taga)2tga(TAGA)4(CAGA)(TAGA)12-11(CAGA)8(taga)2 / -1 / 24
DYS391 / 11 / 10 / (tctg)3(TCTA)11 to 10 / -1 / 31
DYS391 / 11 / 12 / (tctg)3(TCTA)11 to 12 / +1 / 30
DYS391d / 11 / 12 / (tctg)3(TCTA)11 to 12 / +1 / 42
DYS391 / 11 / 10 / (tctg)3(TCTA)11 to 10 / -1 / 20
DYS391 / 10 / 11 / (tctg)3(TCTA)10 to 11 / +1 / 29
DYS392 / 13 / 14 / (AAT)13 to 14(aaat)2 / +1 / 60
DYS393 / 14 / 13 / (GATA)14 to 13 / -1 / 22
DYS393d / 13 / 14 / (GATA)13 to 14 / +1 / 42
DYS393 / 14 / 15 / (GATA)14 to 15 / +1 / 51
DYS437 / 14 / 15 / (tcta)3cta(TCTA)14 to 15(tctg)2(tcta)4 / +1 / 32
DYS437 / 15 / 16 / (tcta)3cta(TCTA)15 to 16(tctg)2(tcta)4 / +1 / 53
DYS438 / 12 / 10,12 / (TTTTC)12 to 10 / -2 / 46
DYS439 / 14 / 13 / (GATA)14 to 13 / -1 / 37
DYS439 / 13 / 12 / (GATA)13 to 12 / -1 / 40
DYS439 / 13 / 12 / (GATA)13 to 12 / -1 / 21
DYS439 / 10 / 11 / (GATA)10 to 11 / +1 / 24
DYS439 c / 13 / 14 / (GATA)13 to 14 / +1 / 36
DYS439 / 13 / 12 / (GATA)13 to 12 / -1 / 23
DYS456 / 15 / 16 / (AGAT)15 to 16 / +1 / 35
DYS456 / 17 / 16 / (AGAT)17 to 16 / -1 / 20
DYS456 / 17 / 16 / (AGAT)17 to 16 / -1 / No data
DYS456 / 16 / 17 / (AGAT)16 to 17 / +1 / 33
DYS456 / 15 / 16 / (AGAT)15 to 16 / +1 / 34
DYS456b / 16 / 17 / (AGAT)16 to 17 / +1 / 32
DYS456 / 16 / 17 / (AGAT)16 to 17 / +1 / 24
DYS456 / 15 / 16 / (AGAT)15 to 16 / +1 / 28
DYS458 / 17 / 18 / (TTTC)17 to 18 / +1 / 36
DYS458 / 15 / 16 / (TTTC)15 to 16 / +1 / No data
DYS458 / 16 / 15 / (TTTC)16 to 15 / -1 / 19
DYS458 / 16 / 17 / (TTTC)16 to 17 / +1 / 37
DYS458 / 17 / 16 / (TTTC)17 to 16 / -1 / No data
DYS458 / 17 / 16 / (TTTC)17 to 16 / -1 / 19
DYS458 / 18 / 17 / (TTTC)18 to 17 / -1 / 64
DYS458 / 18 / 19 / (TTTC)18 to 19 / +1 / 65
DYS458 / 17 / 16 / (TTTC)17 to 16 / -1 / 34
DYS458 / 18 / 19 / (TTTC)18 to 19 / +1 / 41
DYS458 / 18 / 17 / (TTTC)18 to 17 / -1 / 25
DYS458 / 17 / 18 / (TTTC)17 to 18 / +1 / 28
DYS458 / 17 / 18 / (TTTC)17 to 18 / +1 / 38
DYS458 / 16 / 15 / (TTTC)16 to 15 / -1 / 42
DYS635 / 22 / 21 / (TAGA)12 to 11(TACA)2(TAGA)2(TACA)2(TAGA)4 / -1 / 53
DYS635 / 22 / 21 / (TAGA)12 to 11(TACA)2(TAGA)2(TACA)2(TAGA)4 / -1 / 29
DYS635 / 21 / 22 / (TAGA)11 to 12(TACA)2(TAGA)2(TACA)2(TAGA)4 / +1 / 52
DYS635 c / 23 / 22 / (TAGA)13 to 12(TACA)2(TAGA)2(TACA)2(TAGA)4 / -1 / 36
DYS635 / 23,24 / 23e / (TAGA)14 to 13(TACA)2(TAGA)2(TACA)2(TAGA)4 / -1 / 25
DYS635 / 22 / 21 / (TAGA)12 to 11(TACA)2(TAGA)2(TACA)2(TAGA)4 / -1 / 29
Y-GATA-H4 / 11h / 12h / (TCTA)11 to 12(ccta)3(tcta)2taga(tcta)3 / +1 / 19
Y-GATA-H4 / 12h / 11h / (TCTA)12 to 11(ccta)3(tcta)2taga(tcta)3 / -1 / 29
Y-GATA-H4d / 13h / 12h / (TCTA)13 to 12(ccta)3(tcta)2taga(tcta)3 / -1 / 42
Y-GATA-H4 / 13h / 12h / (TCTA)13 to 12(ccta)3(tcta)2taga(tcta)3 / -1 / 53
Y-GATA-H4 / 12h / 11h / (TCTA)12 to 11(ccta)3(tcta)2taga(tcta)3 / -1 / 23

aadjacent repetitive sequence not included in the nomenclature used in the AmpFlSTR® Yfiler® PCR amplificationkit is provided in small letters

b,c2 Y-STR loci mutated in the same father-son pair, respectively (paternity index PI>107 and >106, respectively)

d3 Y-STR loci mutated in the same father-son pair (paternity index PI>107)

e alternatively, a deletion mutation of the entire fragment including allele 24 had occurred

f Age of the father at time of son’s birth

g nomenclature according to the Yfiler manual

h ISFG endorsed nomenclature: +9 repeats, see Gusmao et al. (2006) Forensic Sci Int 157:187–197, Mulero et al. (2006) J Forensic Sci, 51(3):694