Revised 10/16/2018Kevin D. Sarge
Curriculum Vitae
Kevin D. Sarge
I. General Information
Work Address:Department of Biochemistry
University of Kentucky
741 S. Limestone Street, Lexington, KY 40536-0509.
Office Phone: (859) 323-5777
II. Education
Pennsylvania State UniversityB.S.1984Biochemistry
North Carolina State UniversityPh.D.1989Molecular Biology
III. Professional Experience
1984-1989Graduate Research Assistant, Department of Biochemistry,
North Carolina State University, Raleigh, NC (mentor: Dr. Stuart Maxwell)
1989-1993Postdoctoral Fellow, Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL (mentor: Dr. Richard I. Morimoto)
IV. Appointments
1993-1998Assistant Professor, Dept. of Biochemistry, University of Kentucky, Lexington, KY.
1998-2004Associate Professor with tenure, Dept. of Biochemistry, University of Kentucky, Lexington, KY.
2002-Joint-appointee, Graduate Center for Toxicology
2004-Professor with tenure, Dept. of Biochemistry, University of Kentucky, Lexington, KY.
2003-12Director of Graduate Studies for Dept. of Molecular and Cellular Biochemistry.
2015-Assoc. Dean of Graduate Funding & Finance, Graduate School, University of Kentucky.
V. Honors and Awards
1984Phi Kappa Phi, Pennsylvania State University.
1984Phi Lambda Upsilon National Chemical Honor Society, Pennsylvania State University.
1989A.R. Main/Becton Dickinson Award for Outstanding Graduate Achievement,
North Carolina State University.
1997Hans Selye Award for Outstanding Young Investigator
International Stress of Life Meeting, Hungary.
2001 Appointed to Editorial Board, Journal of Biological Chemistry.
2001-2006Wethington Research Scholar of the University of Kentucky.
2006Mentor Recognition Award, Clinical and Translational Science Conference, 6/6/06.
2009-2010University Research Professor, University of Kentucky.
VI. Professional Societies, Grant Reviewing Panels, and Journal Reviewing
Professional Society:
National:
American Society for Biochemistry and Molecular Biology
Federation of American Societies for Experimental Biology
University:
Reproductive Science Forum
Grant Reviewing Panels:
National/International Service:
NASA Developmental and Reproductive Biology Panel (NRA 95-OLMSA-01), 1995.
Israel Science Foundation, 1994.
NSF EPSCoR Research Enhancement Grant, 1999.
NIH Special Emphasis Grant Review Panel, 1999.
NIH NDT study section, Ad hoc reviewer, 2005, 2006, and 2007.
NSF 2007
National Research Council of Canada, 2008 and 2009
University Service:
University of Kentucky Medical Center Research Fund, 1995-1998
Journal Reviewing:
Editorial Board Service:
Member of Editorial Board, Journal of Biological Chemistry, 2001-2006.
Ad hoc Review Service:
Science
Nature
Journal of Biological Chemistry
Molecular and Cellular Biology
Proceedings of the National Academy of Science
Cell Stress and Chaperones
Experimental Cell Research
Gastroenterology
Molecular Pharmacology
Endocrinology
Biology of Reproduction
Gene
Photochemistry & Photobiology.
VII. Talks
University-Wide:
1993 Department of Biological Sciences, Univ. of Kentucky, "Expression of Heat Shock Factor 2 in mouse testis: Second pathway for inducible expression of hsp genes?"
1996 Department of Microbiology and Immunology, UKMC. "Characterization of a molecular thermostat in mammalian cells: the function of heat shock factor as both signal sensor and transcriptional activator of the cellular stress response".
1997 Department of Surgery, UKMC. "HSF as a regulator of hsp expression in vivo”.
2002 Graduate Center for Toxicology, Univ. of Kentucky, "SUMO wrestling with heat shock transcription factors”.
2013 Ag Biotech Program Seminar course, "Mechanism and Function of Gene Bookmarking”.
2014 Ag Biotech Program Seminar course, "Molecular Basis of Human Disease”.
Outreach:
2000 Research presentation and facilities tour to American Cancer Society Donors/Volunteers
Regional:
2016Transylvania University, “Financial Literacy: Paying for Graduate School”
National:
Invited talks at individual Institutions
1994 Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York, NY, "Heat shock factor function and regulation".
2001 Children’s Hospital Medical Center, Division of Critical Care Medicine, Cincinnati, OH. "SUMO wrestling with heat shock transcription factors".
2002 Program in Molecular Biology, at the University of Arkansas College of Medicine, Little Rock, AR.
2002 Department of Molecular and Cellular Biology, University of Arkansas, Fayetteville, AR.
2006 Department of Microbiology and Immunology at Loyola University, Chicago, IL.
Talks at National Meetings:
1995 28th Annual Meeting of the Society for the Study of Reproduction, University of California, Davis, CA. "Modulation of heat shock transcription factor 2 (HSF2) function in testis by regulated alternative splicing of the HSF2 precursor mRNA".
1996 Cold Spring Harbor Meeting on Molecular Chaperones and the Heat Shock Response, Cold Spring Harbor, NY. "Regulation of HSF1 and HSF2 function in mouse cells".
1998 Annual Meeting of the Radiation Research Society and the North American Hyperthermia Society, Louisville KY. "Tissue-dependent regulation of the cellular stress response".
1998Cold Spring Harbor Meeting on Molecular Chaperones and the Heat Shock Response, Cold Spring Harbor, NY. "Regulation of proto-oncogene expression by HSFs".
2000 Invited Speaker, Cold Spring Harbor Meeting on Molecular Chaperones and the Heat Shock Response, Cold Spring Harbor, NY. "Heat shock transcription factor 2 interacts with and regulates protein phosphatase 2A—Potential mechanism for integrating hsp gene regulation with cellular growth control".
2000 FASEB Conference on Protein Phosphatases, Copper Mountain, CO. "Heat shock transcription factor 2 competes with the catalytic subunit for the A subunit of PP2A".
2001 Invited Speaker, Gordon Conference on Stress-induced Gene Expression, New London, CT. "Regulation of HSF1 by stress-induced SUMO-1 modification".
2002 Invited Speaker,Cold Spring Harbor Meeting on Molecular Chaperones and the Heat Shock Response, Cold Spring Harbor, NY. "SUMO-1 modification is a key event regulating the stress-induced vs. constitutive DNA-binding activities of HSF1 and HSF2".
2005 Invited Speaker, Symposium on heat shock factor knockout mouse models. Salve Regina University, Rhode Island. July 16. “Regulation and function of hsp gene bookmarking”.
2005 Invited Speaker, Gordon Research Conference on Stress Proteins in Growth, Development, and Disease, Newport, Rhode Island. July 17-22 “Regulation and function of hsp gene bookmarking”.
2006 Invited Speaker,Cold Spring Harbor Meeting on Molecular Chaperones and the Heat Shock Response, Cold Spring Harbor, NY. "Bookmarking of the hsp90, hsp27, and c-fospromoters by HSF2 during mitosis and its role in modulating constitutive expression of these genes”.
International
1993 Meeting on Heat Shock/Stress Proteins in Multiple Sclerosis, LaToja, Spain, "Distinct functional properties of heat shock factor family members."
1997 Invited Speaker, "Stress of Life" International Meeting on the Cellular Stress Response, Budapest, Hungary. "Regulation of heat shock factors in testis".
2003 Invited Speaker, for the International Congress on Stress Responses in Biology and Medicine, Quebec City, Quebec, September 10-14, 2003.
2005 Invited Speaker, for the International Congress on Stress Responses in Biology and Medicine, Tomar, Portugal, September 23-28, 2005.
2008Invited Speaker and Session Chair for the Jacques Monod Conference entitled “New ideas for an old family: Heat Shock Factors at crossroads between stress, epigenetics and development”, Roscoff, France, September 17-21, 2008.
2009 Invited Speaker, Distinguished Speaker Seminar Series, Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada. “Protein sumoylation and its role in human diseases.” October 21, 2009
VIII. Teaching Activity
Undergraduate Courses
BCH 401G"Fundamentals of Biochemistry", 1994-1995 and 2013-present
HON152Honors course in the Natural and Physical Sciences, 2012, 2013, spring 2016.
BCH 419G“Molecular Basis of Human Disease”. New course I developed that was taught for the first time in Spring 2014. I generated and teach the majority of the lectures for this course, and also serve as course director.
BCH 401G"Fundamentals of Biochemistry", new Evening section, 2013-
BCH 401G"Fundamentals of Biochemistry", new Summer I section, June 2015. Finished development of the course, gave 17 lectures, and served as course director.
PGY 401G"Human Reproduction, Technology, and Society" in which I co-taught with Ok-Kyong Park-Sarge in Spring 2015 and Fall 2015.
Graduate Courses
PGY 590"Cell and Molecular Physiology" 1996-1997.
BCH/BIO 601,"Special Topics in Molecular and Cellular Genetics" 1996.
Hosted Dr. Jerry Workman from the Dept. of Biochemistry and Molecular Biology
at The Pennsylvania State University.
BCH 612"Structure and Function of Proteins and Enzymes" 1994-present.
BCH 615"Molecular Biology and Genetics" 1994 and 1995
PGY 690"Readings in Cell and Molecular Physiology" 1997
BCH 618/619 "Seminar in Biochemistry". 1993-present.
Served as Faculty Advisor for Biochemistry graduate students.
I also attended the Student Seminars and presented my instructive comments to the student speaker on the evaluation form used by the department for this course.
BCH 640"Research in Biochemistry". 1994-2008.
I have served as Faculty Advisor for first-year rotations in my laboratory.
BCH 611"Biochemistry of Nucleic Acids", 2000-2002.
Medical Courses
BCH/MD 819"Cellular Structure and Function: Biochemistry", Instructor from 1997-present.
MD819Began serving as Course Director in 2012 until June 2015.
MD 819 "Cellular Structure and Function: Biochemistry and Genetics", 2013-present. This is the new course combining the two previously separate courses of MD819 (Biochemistry) and MD816 (Genetics).
Curriculum Development
Discipline Coordinator for content area of Biochemistry for the Medical Curriculum: 2012- June 2015.
Proposed and developed new Biochemistry course called BCH419G (Molecular Basis of Human Disease). This course was offered for the first time in Spring 2014.
Proposed and developed new course called BMS100 (Introduction to Biomedical Sciences) for the proposed new undergraduate major in Biomedical Sciences.
Helped in developing new HON 152 course (Sex and Society) that I co-teach with Ok-Kyong Park-Sarge (who is the course director).
Helped in developing new course called PGY401G that I co-teach with Ok-Kyong Park-Sarge (who is the course director).
IX. Advising Activity
Advising Departmental Graduate Students
1994-97 Department of Biochemistry “Graduate Advisory Program Committee”
1994-97Organizer and Coordinator of “Department of Biochemistry Research Forum”
2003-12“Director of Graduate Studies” of the Dept. of Molecular and Cellular Biochemistry.
Graduate Dissertation Committees
Department of Biochemistry
1993-97Member of Dissertation Committee of Mr. AfshinSafavi, Dept. of Biochemistry.
1995-97Member of Dissertation Committee of Mr. Mat Nicholls, Dept. of Biochemistry.
1996-97Member of Dissertation Committee of Ms. Lisa Gothard, Dept. of Biochemistry.
1997-00 Chair of Dissertation Committee of Mr. Michael Goodson, Dept. of Biochemistry.
1996-01Member of Dissertation Committee of Mr. Michael Thompson, Dept. of Biochemistry.
1997-01Member of Dissertation Committee of Mr. William Howard, Dept. of Biochemistry.
1997-01Member of Dissertation Committee of Mr. Juntao Guo, Dept. of Biochemistry.
1999-04Member of Dissertation Committee of Mr. Chad Haynes, Dept. of Biochemistry.
1999-03Chair of Dissertation Committee of Mr. Eric Lubert, Dept. of Biochemistry.
2000-04Member of Dissertation Committee of Ms. Amber Mosely, Dept. of Biochemistry.
2001-05Member of Dissertation Committee of Ms. Lisa Kelly, Dept. of Biochemistry.
2005-08Chair of Dissertation Committee of Ms. Yuqian Zhang, Dept. of Biochemistry.
2005-07Chair of Dissertation Committee of Ms. Jianing Yang, Dept. of Biochemistry.
2005-10Member of Dissertation Committee of Ms. Jamie Cantrell, Dept. of Biochemistry.
2009-Member of Dissertation Committee of Mr. Brent Hackett, Dept. of Biochemistry.
2013-14Chair of Master’s Thesis Committee of Mr. John Ross (MS in MS student)
2014-15Member of Master’s Thesis Committee of Mr. Justin Werker (MS in MS student)
University-Wide
1997-99Member of Dissertation Committee of Ms. Christa Behrendt,
Dept. of Veterinary Biosciences
1997-01Member of Dissertation Committee of Mr. David Peyton,
Dept. of Microbiology and Immunology.
1998-02Member of Dissertation Committee of Ms. Nadia El-Guendy,
Dept. of Microbiology and Immunology.
2003-07Member of Dissertation Committee of Mr. Ran Wu, Dept. of Pharmacology.
2002-07Chair of Dissertation Committee of Ms. Hollie Skaggs, Graduate Center for Toxicology.
2003-08Chair of Dissertation Committee of Ms. Lynea Murphy, Graduate Center for Toxicology.
2005-09Chair of Dissertation Committee of Ms. Jie Zhang, Graduate Center for Toxicology.
2005-07Member of Dissertation Committee of Ms. Sathya Ramasamy, Dept. of Micro.and Immunology.
Advising for Graduate Students and Postdoctoral Fellows in Research
Graduate Students:
Michael Goodson1997-2000BiochemistryPostdoc at UC-Davis
Lisa Gothard1996-1997BiochemistryChemistry Teacher, Sandy Valley High School
Eric Lubert1999-2003BiochemistryStaff Scientist, Battelle Inc.
Hollie Skaggs2002-2007ToxicologyScientist, Covance Inc.
Lynea Murphy2003-2008ToxicologyPostdoc at EPA
Yuqian Zhang2005-2008BiochemistryPostdoc at UCLA
Jie Zhang2005-2009ToxicologyPostdoc at Mayo Clinic, MN
Jianing Yang2005-2007BiochemistryNutritional Sciences Program
Postdoctoral Fellows:
Katherine Cullen1994-1995Vanderbilt UnivAssistant Professor at
Transylvania Univ
Mary Brown1997-1998Univ. of Conn.Domaine Carneros Winery
Yiling Hong1997-2001Univ. KentuckyAssistant Professor
University of Dayton
Chris Mayhew2001-2002Univ Wolverhampton Research Associate
UKUniversity of Cincinnati
Hongyan Xing2001-2011Peking UnivCurrent Postdoc
Chad Wilkerson2002-2009Louisiana State Univ.Res. Associate
Univ. of Kentucky
Roland Hilgarth2001-2005Univ. KentuckyAllylix Inc.
Undergraduate Student enrolled in BCH 395, "Independent Work in Biochemistry"
Emily Tan1997-1998Univ. KentuckyMedical Student at UK
Amin Akhlaghi1997-1999Univ. KentuckyMedical Student at
Georgetown Univ.
Michelle Brammer2005-07Univ. KentuckyMedical Student,
University of Indiana
Advising for BCH Student Seminar (BCH 618/619)
From 1994-present I served as Faculty Advisor for many Biochemistry graduate students for their seminars in this course. I advised each student on the selection of the seminar topic and had the students present at least two practice seminars to me so that I could provide constructive criticism to improve their presentation.
Advising for Graduate Students during Laboratory Rotation (BCH 640)
From 1994 I have served as Faculty Advisor for many Biochemistry or IBS graduate students during their first-year rotation in my laboratory. I advised each student on what I think are the fundamental important attributes required of a successful scientist, and advised them of the transition they must make in going from a primarily classroom-based education (undergraduate) to the complex mixture of independent research and more self-directed learning style required in graduate school.
X. Administrative Activity and University Service
Department of Biochemistry
1994Department of Biochemistry “Brochure Committee”
Involved in revising the Biochemistry Department brochure for graduate recruiting.
1994-95Coordinator of “Department of Biochemistry Seminar Program”
As Coordinator, applied for and received $700.00 from the Graduate Program Enrichment Fund to help cover the costs of the seminars of Dr. Judith Bond
(joint with M&I) and Dr. Stanley Parsons ($350.00 for each speaker).
1994-97 Department of Biochemistry “Graduate Advisory Program Committee”
1994-97Organizer and Coordinator of “Department of Biochemistry Research Forum”
1999-01Department of Biochemistry (later IBS) “Graduate Recruiting Committee”
2007Directed Department Summer Research Retreat held at Pine Mountain State Park.
2003-12Director of Graduate Studies for the Dept. of Molecular and Cellular Biochemistry.
College of Medicine
1996UKMC Member for LCME Accreditation Site Visit
1996Presenter at UKMC Graduate Forum on Grant Writing (Organizer: Dr. Thomas Getchell)
"Essentials of a Successful Grant Application"
1996-98College of Medicine Research Committee
Involved in Development of Research Productivity Model for Faculty Evaluation
1996College of Medicine Task Force on Research (Chair, Dr. Thomas Getchell)
1997Internal Grant Reviewer for Small Equipment Grant proposals
1998-01Medical Center Research Advisory Committee
2001-12Oversight Committee for Integrated Biomedical Sciences Graduate Program
2002UKMC Member for LCME Accreditation site visit
2007Member of committee for 6 year review of Pathology and Laboratory Medicine, UK.
2009Member of committee for 6 year review of Graduate Center for Nutritional Sciences, UK.
2012-Course Director for MD819 (Medical Biochemistry).
2012-Course Director for MD819 (newly combined Medical Biochemistry and Genetics course).
2012-Member of Executive Committee for Horizontal Integration (Medical Curriculum).
University-Wide
1995-96Coordinator of the Reproductive Biology Forum for the NIH Reproductive Sciences Training Grant (P.I. Dr. Delwood Collins)
1997 Director for the 17th Annual Symposium in Reproductive Sciences, Lexington, KY
1997-98 Co-Director of Graduate Studies in Cell and Molecular Biology
1997-00Chemical Safety Committee of the University of Kentucky
2000 Research presentation and facilities tour to American Cancer Society donors and volunteers
2002 Outside examiner for defense of Neetu S. Dembla, for the Graduate School.
2007Member of selection committee for Bryan Professorships in Chemical and Materials Engineering.
2010-11Served on an Investigative Committee formed by Dr. James Tracy, Vice President for Research.
2010-12Served as a Member of the University Senate.
2011-12Presented instructional seminars on strategies to teach understanding of scientific method and value of research to individuals and society to a meeting of Fayette County Elementary Science teachers. This was followed a pilot series of interactive one hour presentations to combined 4th grade class groups at 5 Fayette County Elementary schools (over 500 students and twenty 4th grade teachers), designed to teach the students the things I discussed in the meeting with the Fayette county teacher group (essentials of the scientific method, and the value of science knowledge and research for individuals and society as a whole).
2013-14Member of the Faculty Advisory Work Team for RCM Budget Model.
2015-Began in June as Associate Dean of Finance, Funding, & Analytics in UK Graduate School.
XI. Scholarly Productivity
Publications (includes Research Papers, Reviews/Book Chapters, and Abstracts)
Research Papers
1.Maxwell, E.S. and Sarge, K.D. (1988) A simple and rapid method for the preparation of homologous DNA oligonucleotide hybridization probes from heterologous gene sequences and probes. Bioc. Biop. Res. Commun. 157: 154-157.
2.Sarge, K.D. and Maxwell, E.S. (1991) Intermolecular hybridization of 5S rRNA with 18S rRNA: Identification of a 5'-terminally-located nucleotide sequence in mouse 5S rRNA which base-pairs with two specific complementary sequences in 18S rRNA. Biochim. Biophys. Acta 1088: 57-70.
3.Sarge, K.D. and Maxwell, E.S. (1991) Evidence for a competitive-displacement model for the initiation of protein synthesis involving the intermolecular hybridization of 5S rRNA, 18S rRNA, and mRNA. FEBS Lett. 294: 234-238.
4.Mosser, D.D., Kotzbauer, P.T., Sarge, K.D., and Morimoto, R.I. (1990) In vitro activation of heat shock transcription factor DNA-binding by calcium and biochemical conditions that affect protein conformation. Proc. Natl. Acad. Sci. USA 87: 3748-3752.
5.Sarge, K.D., Zimarino, V., Holm, K., Wu, C., and Morimoto, R.I. (1991) Cloning and characterization of two mouse heat shock transcription factors with distinct inducible and constitutive DNA-binding ability. Genes Dev. 5: 1902-1911.
6.Sistonen, L., Sarge, K.D., Phillips, B., Abravaya, K., and Morimoto, R.I. (1992) Activation of heat shock factor 2 during hemin-induced differentiation of human erythroleukemia cells. Mol. Cell. Biol. 12: 4104-4111.
7.Sarge, K.D., Murphy, S.P., and Morimoto, R.I. (1993) Activation of heat shock gene transcription by HSF1 involves oligomerization, acquisition of DNA binding activity, and nuclear localization and can occur in the absence of stress. Mol. Cell. Biol. 13: 1392-1407.
8.Kroeger, P.E., Sarge, K.D., and Morimoto, R.I. (1993) Mouse heat shock transcription factors 1 and 2 prefer a trimeric binding site but interact differently with the hsp70 heat shock element. Mol. Cell. Biol. 13: 3370-3383.
9.Jurivich, D.A., Sistonen, L., Sarge, K.D., and Morimoto, R.I. (1994) Arachidonic acid is a potent modulator of human heat shock gene transcription. Proc. Natl. Acad. Sci. 91: 2280-2284.
10.Sistonen, L., Sarge, K.D., and Morimoto, R.I. (1994) Human heat shock factors 1 and 2 are differentially activated and can synergistically induce hsp70 gene transcription. Mol. Cell. Biol. 14: 2087-2099.
11.Sarge, K.D., Park-Sarge, O.-K., Kirby, J. D., Mayo, K. E., and Morimoto, R.I. (1994) Expression of Heat Shock Factor 2 in mouse testis: potential role as a regulator of heat-shock protein gene expression during spermatogenesis. Biol. Reprod. 50: 1334-1343.
12.Murphy, S.P., Gorzowski, J.J., Sarge, K.D., and Phillips, B. (1994) Characterization of constitutive HSF2 DNA-binding activity in mouse embryonal carcinoma cells. Mol. Cell. Biol. 14: 5309-5317.
13.Mathur, S.K., Sistonen, L., Brown, I.R., Murphy, S.P., Sarge, K.D., and Morimoto, R.I. (1994) Deficient induction of human hsp70 heat shock gene transcription in Y79 retinoblastoma cells despite activation of heat shock factor 1. Proc. Natl. Acad. Sci. (USA) 91: 8695-8699.
14.Fawcett, T.W., Sylvester, S.L, Sarge K.D., Morimoto, R.I., and Holbrook, N.J. (1994) Effects of neurohormonal stress and aging on the activation of mammalian heat shock factor 1. J. Biol. Chem. 269: 32272-32278.
15.Goodson, M.L., and Sarge, K.D. (1995) Heat-inducible DNA-binding of purified heat shock transcription factor 1 (HSF1). J. Biol. Chem. 270: 2447-2450.
16.Sarge, K.D., Bray, A.E., and Goodson, M.L. (1995) Altered stress response in testis. Nature 374: 126.
17.Goodson, M.L. and Sarge, K.D. (1995) Regulated expression of heat shock factor 1 (HSF1) isoforms with distinct leucine zipper arrays via tissue-dependent alternative splicing. Bioc. Biop. Res. Commun. 211: 943-949.