Supplement Table1. the Content and Concentration of Total RNA in Rats Hippocampus

supplement Table1. The content and concentration of total RNA in rats’ hippocampus

Sample IDa / OD260/280 Ratiob / OD260/230 Ratio / Conc. (ng/μl) / Volume (μl) / Quantity (ng) / QC result Pass or Fail
A1 / 2.01 / 2.29 / 1138.94 / 20 / 22778.80 / Pass
A2 / 2.00 / 2.32 / 1109.02 / 20 / 22180.40 / Pass
A3 / 2.01 / 2.29 / 1051.78 / 15 / 15776.70 / Pass
B1 / 2.05 / 2.22 / 1087.30 / 20 / 21746.00 / Pass
B2 / 2.05 / 2.24 / 1466.11 / 20 / 29322.20 / Pass
B3 / 2.05 / 2.24 / 1348.31 / 15 / 20224.65 / Pass
C1 / 2.02 / 2.33 / 1187.68 / 20 / 23753.60 / Pass
C2 / 2.06 / 2.29 / 992.98 / 20 / 19859.60 / Pass
C3 / 2.07 / 2.08 / 1123.93 / 20 / 22478.60 / Pass
D1 / 2.04 / 2.26 / 1437.13 / 20 / 28742.60 / Pass
D2 / 2.02 / 2.33 / 1284.24 / 20 / 25684.80 / Pass
D3 / 2.03 / 2.32 / 1147.28 / 20 / 22945.60 / Pass

a,A1~A3: Total RNA of rat brain hippocampus in sham group at 7d after microwave exposure. B1~B3: Total RNA of rat brain hippocampus in 30mW/cm2 group at 7d after microwave exposure. C1~C3: Total RNA of rat brain hippocampus in sham group at 14d after microwave exposure.D1~D3: Total RNA of rat brain hippocampus in 30mW/cm2 group at 14d after microwave exposure.

b, For spectrophotometer, the O.D. A260 /A280 ratio should be close to 2.0 for pure RNA (ratios between 1.8 and 2.1 are acceptable). The O.D. A260/A230 ratio should be more than 1.8.

supplement Table2. miRNA sequence for quantitative real-time PCR

miRNA / target sequence
rno-miR-125b-5p / UCCCUGAGACCCUAACUUGUGA
rno-miR-338 / UCCAGCAUCAGUGAUUUUGUUGA
rno-miR-19a / UGUGCAAAUCUAUGCAAAACUGA
rno-miR-125a-5p / UCCCUGAGACCCUUUAACCUGUGA
rno-miR-9 / UCUUUGGUUAUCUAGCUGUAUGA
rno-miR-181a / AACAUUCAACGCUGUCGGUGAGU
rno-let-7b / UGAGGUAGUAGGUUGUGUGGUU

Supplement Table3. Predicted targets of modulated miRNAs at 7d after microwave exposure.

miRNAs / Target genes
Up-
regulated / rno-miR-21a / AP1B1,FMO2, NAP1L3, NPC2, PAF1, PDHB, PTF1A, REG3A, RHOBTB2, SLC7A3, SMCP, SNCA, TESK2, TFG, UBE2I, VAPA, ZFP207
rno-miR-340-3p / BLCAP, RBX1, SEPT9, TOR1AIP1
rno-let-7d a / IL10, ATP2B1, SYT5, NR4A2, AQP11, UCP3
rno-miR-146a / CASK, HRSP12, P2RY1, PLK2, SLC25A14, SLC38A5, STRBP, TINF2
rno-miR-320 / ARF1, CADPS, CLIC6, CYP1A2, DBN1, DNASE1L1, GSPT1, HPCAL1, HPGD, ICMT, INPP4A, KCNS3, KIF22, LIG3, ODF4, PAH, PHF1, PON2, PPM1B, STAT4, THBD, YWHAH
rno-miR-423 a / SLC8A3, SYT1, MAPK14, LYN, MMP9,ATP6V0E2
rno-miR-328a / RBFOX1, BCL2L2, TCF7L2, FOXO4, ESCO1
rno-miR-361 / AMACR, CASP7, CPOX, CRY1, DHRS9, GAK, MAS1, MTRR, PDK1, PGAM5, RAD23A, RNF141, RRAGB, SLC10A1, SSTR2, STAU1, STX7, UBE2G1
rno-miR-465 a / ST7, TNFRSF8, NOVA1, CALB1, CDKN1B, SNAP25, CLDN1
rno-miR-378 / GLUD1, AKAP12, BCL2L2, NOTCH4, COX15,
rno-miR-494 / MMP12, RIMS1, TNF, SYT5, SMAD1, SYT1, RAF1
rno-miR-505 a / ATP1B2, SV2B, EGR2
Down-
regulated / rno-miR-181a / ACSL1, BTNL8, CBX7, CMTM8, CNTN4, CXCL10, CYR61, DNAJA1, DNAJA4, EIF4A2, ESM1, GPSM1, LOC362855, LRRN1, NCBP1, NNT, OAT, PDAP1, PI4K2B, PLEKHA3, PRKCD, PRKRA, RECQL, SYNPR, TIMP3, TMEM27, TMEM33, VRK3, YWHAG
rno-miR-27b / ADORA2B, AQP11, CABP1, CHKA, CNGA2, COG7, FRS3, NAP1L2, NFE2L2, NLN, NR2F6, PLK2, PPARG, RGS1, RGD1307100, SMAD5, XPNPEP2
rno-miR-136 / BBS2, CP, FBP2, HNRPH1, MKS1, NNT, RAB11A, RIMS2, RPUSD4, SDPR, SLC7A3, SLCO2A1, TRAK2, ZHX1
rno-miR-29b / ABCB6, AGRN, CCNA2, COL3A1, COL5A3, CSPG4, DNAJB11, DUSP2, ELF2, LN, GPR37, HAS3, HBP1, HMGCR, IFI30, IREB2, LYSMD1, MOAP1, NFIA, OLR1271, PIGC, PMP22, PPIC, RAB30, SERPINA5, SMS, SNX24, TDG
rno-miR-376b-5p / ACTN4, LOX5AP, CTBS, FGB, LRRFIP1, PKIA, PSMA5, SERINC3, SLC30A4, TMEM66, TYROBP, ZFP354A
rno-miR-219-5p / ARHGAP20, DAZAP1, FAHD1, ING3, KLRA22, LAPTM4A, MYOCD, REM1, RIMS1, SLC16A7, SLC31A1, TMEM98
rno-miR-30a / ABCG5, ACTC1, ARID4B, BECN1, BTNL7, CADPS, CALM1, CDC37L1, CRHBP, CTSM, GABRB1, GALNT3, GNAI2, GPR149, IDH1, IL21R, KCNA4, LOC498606, RRC17, MTA1, NEFL, NT5E, OCIAD1, OMG, PGM1, PPARGC1B, RGD1359108, RHEBL1, RQCD1, RRAD, SCN2A1, SCN8A, SLC5A3, SLC7A10, SNAI1, STK39, SYTL1, TIA1, UBE2I, UNC5C
rno-miR-126 a / ADI1, ARFIP1, ATN1, AXIN1, BRD8, BZW1, DIRC2, FGFRL1, GALNT13, GGH, GRIK1, LOC606294, MDH1, NEFL, NIT1, NR2F1, OMD, TMEM33
rno-miR-19b / ARFIP1, CAR8, CBFB, CCDC51, CCNL1, CD69, CDC26, DPYSL5, ENTPD5, FMR1, ID4, KCNA4, LAPTM4A, LOC498350, MAPK6, MARCH2, PDE5A ,RAF1, RAP1A, RBMS1, RGD1308470, RGD621098, RHEBL1, RNF167, SCN1B, SEPT7, SLC31A2, SMARCA2, SMARCD2, SNX7, ST3GAL5, TFB2M, TOR1AIP2, VPS4B, ZC3H8
rno-miR-142-3p / SNED1, ATG16L1
rno-miR-130a / PPARG, MAPK14, SMAD1
rno-let-7f / ADRB2,ADRB3, APBB3, BZW1, CTBS, EHHADH, IKBKAP, LOC497899, PTAFR, PVR
rno-miR-381 / TRAM1, SMAD2, BDNF
rno-miR-128 / BDNF, SIRT6
rno-let-7d / APBB3, CTBS, EHHADH
rno-miR-107 / TRAM1, BDNF, GLUD1
rno-miR-592 / TCF20, TGFB2, IGF1
rno-miR-101a / SCNN1A, ERMP1
rno-miR-19a / ARFIP1,CAR8,CAST,CBFB, CCDC51, CCNL1, CD69, CDC26, DBN1, DPYSL5, ENTPD5, ID4, LAPTM4A, LOC498350, MARCH2, PDE5A, RAF1, RAP1A, RBMS1, RGD1308470, RGD621098, RNF167, SCN1B, SEPT7, SMARCA2, ST3GAL5, TFB2M, TOR1AIP2, VPS4B, WEE1, ZC3H8
rno-let-7c / ADRB3,APBB3, BZW1, EHHADH, GIPC1, IKBKAP, KLRC1
rno-miR-3597-5p
rno-miR-103 / TRAM1, BDNF
rno-miR-30e / ABCG5, ACTC1, BECN1, BTNL7, CADPS, CALM1, CDC37L1, CHKA, CTPS2, GABRB1, GALNT3, GNAI2, GPR149, GPR64, IDH1, IL21R, KCNA4, LOC498606, LRRC17, MTA1, NEFL, NT5E, OCIAD1, OMG, PGM1, PPARGC1B, RAB38, RGD1359108, RHEBL1, RNF2, RQCD1,
RRAD, SCN2A1, SCN8A, SLC5A3, SLC7A10, SNAI1, SNAPAP, SYTL1, TIA1, UBE2F, UBE2I, UNC5C,
rno-miR-142-5p / AARD,ACTN4, APBB3, BECN1, BTG3, BTG4, CAPN7, CBWD1, ETFDH, FBXO9, FMR1, HERPUD1, LGALS8, LOC500118, LUC7L, MNAT1, NQO2, NRG1, PLEKHA3, PSMB9, PSME2, ROD1, SLC18A2, TDO2, TMEM54, UBE2A, UXS1, VRK1
rno-miR-338 / B4GALT7, CCT5, COQ6, FKBP1A, GNA12, HMOX2, LOC298139, MAFB, MORF4L2, MTVR2, NARS
rno-miR-374 / ACTN4, BBS7, CBWD1, CTBS, EREG, ETFA, FMR1, GADD45A, GSTT2, IL2, MSX1, NTF3, OAT, PNRC1, RAB38, RGN, RGS1, RNASE4, RTCD1, SERPINI1, TM9SF1, UQCRH, VSNL1, WNT5A
rno-miR-181c / ACSL1, BTNL8, CBX7, CD4, CMTM8, CYR61, DNAJA1, DNAJA4, EHD4, EIF4A2, ESM1, HPGD, NCBP1, NNT, OAT, PDAP1, PI4K2B, PLEKHA3, PRKCD, PSIP1, RECQL, SYNPR, TIMP3, TINAG, TMEM27, TNFRSF11B, VRK3
rno-miR-410 / DMTF1, ZFP52
rno-miR-9 / AARD, ABCB6, ACOT7, ADCY5, CALB2, CNNM2, CNTN3, CTHRC1, CYP2U1, EIF1A, EMB, ERG, GALNT3, IFT80, ITM2B, KCNJ14, LECT1, NAP1L1, ONECUT1, PALMD, SNX7, STK3, TESK2, TSHR,
rno-miR-29c / ABCB6, AGRN, CCNA2, CHFR, COL3A1, COL5A3, CSPG4, DNAJB11, DUSP2, ELF2, ELN, GPR37, HAS3, HBP1, HMGCR, IFI30, IREB2, LYSMD1, NFIA, OLR1271, PIGC, PMP22, PPIC, RAB30, SERPINA5, SMS, SNX24, TDG
rno-let-7a / ADRB3, APBB3, BZW1, CTBS, EHHADH, IKBKAP, KLRC1, PVR
rno-miR-543 a / ABCB1A, ARG1, CMTM8, CSDA, EEF1A1, IK, LOC362855, LRRC8D, MGC95152, NBR1, NRCAM, PJA2, PLA2G4A, PON1, PPP1CB, PSPC1, RGD1305679, SH3GLB1, SLC18A2
rno-miR-129-2 a / CCP110,AIF1L
rno-miR-218a / KCNJ6, MOSPD1
rno-miR-488 / ERMP1, PHYHIP
rno-miR-194 / ADAM17, AQP8, BIRC2, DMP1, ERGIC2, GCNT3, GYG1, LUC7L, MED4, NXPH1, PAICS, POLD3, RGD1308059, RGD1308470, RGD1309228, SUMO2, TFB2M, ZFP111
rno-miR-25 / ADCY3, ADM, ANK3, ARPC5, BBS7, C3AR1, CCNH, CD69, CLDN11, COL1A2, DNAJC4, ERGIC2, FMR1, GAP43, GLRA1, GRIA3, GRP, IBSP, MGLL, MPI, NELF, NOX4, PACSIN2, PIGV, RGD1307100, RGD1308059, SERPINB6A, SLC32A1, UGP2, WRNIP1
rno-miR-181a-1 a / TTC7B, DGKB
rno-miR-137 / POLR1A, TUT1
rno-miR-23b / ADI1, ATP6V1E1, BTNL7, BTNL8, CCK, CCNH, CNDP2, CRIPT, CTSQL2, DPH5, DYT1, ENTPD5, FUT4, GPR64, KATNAL1, KCNK3, LOC499602, MARCKSL1, MCOLN2, MGC114246, NANP, NDUFS4, PKIA, PLEKHA3, PPIF, RNF146, RXRG, ST18, STAT5B, STX17, TFPI2, TMPRSS5, TPD52L2, TXNDC9, UQCRFS1, ZFP423
rno-miR-29a / ABCB6, BDH1, CCNA2, CHFR, COL3A1, COL5A3, CSPG4, DNAJB11, DUSP2, ELN, GPR37, HAS3, HBP1, HMGCR, IFI30, IREB2, LYSMD1, MOAP1, NFIA, PIGC, PMP22, PPIC, RAB30, SERPINA5, ZFP354A
rno-miR-20b-5p / AADAC, ARID4B, CAMK2N1, CASP8, CCT6A, CDC37L1, CDK2, CLCC1, COPS4, CP, CYB5D2, DCUN1D3, DDX5, DMTF1, GALR1, GRAMD1A, GZMB, HAS2, HCRTR2, IGSF10, KPNA2, LACTB2, LAPTM4A, LOC499331, LOC500118, MYCN, NAGK, NR4A3, NUP35, PAFAH1B2, PCDHA3, PKNOX1, PLEKHA3, PLSCR3, PPP6C, RAB30, RBBP7, RGD1306227, RGD1308127, RGD1359108, RIPK3, SAR1B, SCAMP2, SDS, SLC2A4, SLC6A15, SSX2IP, STK17B, TBXAS1, TLE4, TRIM3, TRPV6, VDAC2, WEE1
rno-miR-152 / DMTF1, ERMP1
rno-let-7b / ADRB3, APBB3, BZW1, EHHADH, GIPC1, IKBKAP, KLRC1
rno-miR-99a / KBTBD8, AP1AR
rno-miR-32 / ADCY3, ADM, ANK3, BBS7, CCNH, CD69, CLDN11, COL1A2, DKK3, DNAJB9, DNAJC4, ERGIC2, GAP43, GLRA1, GRAMD3, GRIA3, GRP, IBSP, ITPR1, KLF4, MGLL, MPI, NOX4, PIGV, PRKAR1B, RGD1307100, RGD1308059, SERPINB6A, SLC32A1, SMAD7, TOB1, UGP2, WRNIP1
rno-miR-425 / ALG5, ANXA11, ART3, ATP5G3, CDC5L, CLDN8, CSDA, CYP26A1, DTWD1, DUSP2, HDLBP, MMP10, NCDN, TPH2
rno-miR-138-1 a / MSL2, TMEM57
rno-miR-224 / ATP12A, AZIN1, CAST, CCKBR, DLL1, FANK1, FOXA2, GPC4, HHEX, KCNA2, LOC500300, MFAP3, PIGC, POLR2G, PSAP, RNF38, SCOC, WSB1, ZFP207
rno-miR-34a / BMP3, CAPN8, COLQ, CSF1R, ENO3, FBXO30, INHBC, LDHA, NOL10, NONO, PCK1, RTN2, SVOP, TMEM109, TMEM22, TP53, VAMP2, VDR
rno-miR-29c a / PAH
rno-miR-582 / LRAT, PISD
rno-miR-125b-5p / MMP9, STAT3
rno-miR-24 / CRAT, DLGAP4, FLCN, GGA1, GRIA3, HNMT, MED4, NDST1, OTUD5, PTPN9, RGD1305689, SSTR1, STAT2
rno-miR-138 / CNOT8, MMP7, NELF, NPPC, PPIC, PXMP4, RARA, RQCD1, TRAM1
rno-miR-369-5p / MS4A2
rno-miR-350 / C1GALT1C1, CROT, DCUN1D5, DOC2A, EPB4.1L5, IK, IL24, KCNH8, KLHL24, LACTB2, LAPTM4A, LUM, MAGEH1, MDH1, MTDH, NEDD9, NR0B1, OGT, PDE1C, PRPSAP2, PRR3, RAD23B, SLC17A6, SNIP1, SPRY2, STRBP, SULT1B1, ZCCHC12
rno-miR-497 / ARL2, B4GALT7, BTRC, CASK, CCDC19, CDC25A, CHPT1, DLL1, DUSP9, EIF2B2, ESAM, G7C, GLUD1, HSD17B7, KCNC2, MAP2K1, MGC114483, MKKS, MLYCD, MYT1L, PFKFB4, PHKG2, PPP6C, PTH, RGD1311739, RNF10, RTN3, SERINC3, SIAH1A, SLC16A6, SNX16, STXBP3, TBP, TMEM55A, USP14, WEE1, WIPI2
rno-miR-384-3p / RHOV, TRAM1
rno-miR-542-3p / DMTF1, TRPC4
rno-miR-125a-5p / MMP9, STAT3
rno-miR-409-5p / ARID4B, CDC37L1, GALNT5, PKIB, RGN, TSHR
rno-miR-499 / ANGPTL2, CTSE, CYB5R4, H2AFZ, MAPK6, MRPL24, MRPL37, PDE1C, PGAP1, RCN2, RRAGB, SDHD, TMED9, UBE2V2
rno-let-7i a / DLX5
rno-miR-540 a / RGL3
rno-miR-129-1a/2 a / RCHY1
rno-miR-344a-3p / LRAT
rno-miR-382 a / BTRC, CAT, DLL3, MAD2L1BP, MAPK6, NFYB, OTC, PDIA5, SDC2, SSX2IP, XBP1
rno-miR-125b-3p / MMP9, STAT3
rno-miR-181d / ADM, BTNL8, CMTM8, CYR61, DNAJA1, DNAJA4, IL7, LRRN1, NCBP1, OAT, PDAP1, PDE5A, PI4K2B, PLEKHA3, PRKCD, PRKRA, PSIP1, RECQL, RGD1308470, RNF34, SIPA1L2, STC1, SYNPR, TIMP3, VRK3

The “a” tells us that miR a is considered a “minor” product. It is often inferred that miR a is non-functional, and it was noticed that miR a sequences in general magically disappear in most pictures of miRNA biogenesis, while the dominant arm is magically incorporated into the RISC complex.
supplement Table4. Predicted targets of modulated miRNAs at 14d after microwave exposure.

miRNAs / Target genes
Up-
regulated / rno-miR-30a a / CALM1, IDH1, PGM1, SYTL1
rno-miR-344a-3p / LRAT, STAU1
Down-
regulated / rno-miR-337 a / CD5, NR4A3
rno-miR-185 / ANXA2, ARCN1, ATP6V1F, ATXN1, DLST, ERCC3, GALNT5, LGALS3, MGC93975, NR1D1, NTRK2, PTH, RAB3D, RGD1311946, TAGLN3, TARS, THTPA, ZFP384
rno-miR-127 a / ELOVL6, GLCCI1
rno-miR-129 / AGA, CACYBP, CIB2, FGF13, FHL1, GOT2, LOC500445, NR2C2, PLCE1, RGD1359460, RPS6KB1, SFMBT1, SMPX, SULT1A1, TFB1M, UGT8
rno-miR-28 / ADM2, CD28, IL13RA1, SLC38A1, SRPR, SSRP1, TXNL1
rno-miR-328a / RBFOX1, BCL2L2, TCF7L2, FOXO4, ESCO1
rno-miR-674-5p / CLTA, HRH4, HSD11B2, IGF1R, KCNS3, MAP2K1IP1, RGD1309676, SEC61A1, TNIP2, UCHL5, UGT8
rno-miR-672 a / UNC5C
rno-miR-29a a / ACBD6, ATP6V1C1, AZIN1, CCT2, COL4A4, DONSON, DPYS, DUSP11, GRID2, IK, LETM1, MCCC2, MFAP3L, NSDHL, OBP1F, POU1F1, PRLHR, RGD1359600, SLC16A7
rno-miR-330 / BCL2L2, DPP7, EBF1, FXYD7, GIMAP4, GPI, JMJD6, NINJ1,
RGD1309540, RGD1310127, RGD1359529, SLC39A8, SLC5A3, TNFRSF14
rno-miR-183 / AP3M1, BTG1, GM2A, HAO2, KATNA1, NPC2, PLEKHA3, PPP2CB, PPP2R1A, PPP2R2A, PRKCH, QDPR, RCN2, RPL15, SNX1, SNX7, SPRY2, SSRP1, TESTIN, TMPO, WBP11, ZFP451
rno-miR-423 a / DYNC1I2
rno-miR-320 / ARF1, CADPS, CLIC6, CYP1A2, DBN1, DNASE1L1, GSPT1, HPCAL1, HPGD, ICMT, INPP4A, KCNS3, KIF22, LIG3, ODF4, PAH, PHF1, PON2, PPM1B, STAT4, THBD, YWHAH
rno-miR-154 / ADIPOR1, ALDH1A2, C6, CDCA4, CHRM2, COG7, COPS2, IL18, TMEM35, TXNDC9
rno-miR-329 / BTNL7, CDCA4, CRMP1, CYP4B1, IL13RA1, LCP2, RBP2, TMED9, TPBG
rno-let-7b / ADRB3, APBB3, BZW1, EHHADH, GIPC1, IKBKAP, KLRC1

The “a” tells us that miR a is considered a “minor” product. It is often inferred that miR a is non-functional, and it was noticed that miR a sequences in general magically disappear in most pictures of miRNA biogenesis, while the dominant arm is magically incorporated into the RISC complex.

supplement Table5. Predicted pathways of target genes at 7d after microwave exposure.

Code / Definition / Genes
rno04146 / Peroxisome / ACSL1、AMACR、CAT、CRATCROT、EHHADH、IDH1、MLYCD
rno04721 / Synaptic vesicle cycle / ATP6V1E1、RIMS1、SLC17A6、SLC18A2、SLC32A1、VAMP2
rno04114 / Oocyte meiosis / ADCY3、ADCY5、BTRC、CALM1、CDC26、CDK2、ITPR1、MAP2K1、PPP1CB、RBX1
rno00020 / Citrate cycle TCA cycle / IDH1、MDH1、PCK1、PDHB、SDHD
rno04730 / Long-term depression / GNA12、GNAI2、GRIA3、ITPR1、MAP2K1、PLA2G4A、RAF1
rno00380 / Tryptophan metabolism / CAT、CYP1A2、EHHADH、TPH2
rno04020 / Calcium signaling pathway / ADCY3、ADORA2B、ADRB2、ADRB3、CALM1、CCKBR、ITPR1、PDE1C、PHKG2、PTAFR
rno04722 / Neurotrophin signaling pathway / CALM1、MAP2K1、NTF3、PDK1、PRKCD、RAF1、RAP1A、TP53、YWHAH
rno05031 / Amphetamine addiction / ADCY5、CALM1、GRIA3、PPP1CB、SLC18A2
rno00520 / Amino sugar and nucleotide sugar metabolism / MPI、NAGK、NANP、PGM1、UGP2
rno00512 / Mucin type O-Glycan biosynthesis / C1GALT1C1、GALNT13、GALNT3、GALNT5、GCNT3
rno05134 / Legionellosis / ARF1、CASP7、CASP8、EEF1A1、SAR1B
rno03420 / Nucleotide excision repair / CCNH、POLD3、RAD23A、RAD23B、RBX1
rno04350 / TGF-beta signaling pathway / ID4、INHBC、RBX1、SMAD5、SMAD7
rno04964 / Proximal tubule bicarbonate reclamation / GLUD1、MDH1、PCK1
rno04914 / Progesterone-mediated oocyte maturation / ADCY3、ADCY5、CCNA2、CDC25A、CDC26、CDK2、GNAI2、MAP2K1、RAF1
rno04666 / Fc gamma R-mediated phagocytosis / ARPC5、ARPC5L、MAP2K1、MARCKSL1、PLA2G4A、PRKCD、RAF1
rno04710 / Circadian rhythm – mammal / BTRC、CRY1、RBX1
rno05216 / Thyroid cancer / MAP2K1、PPARG、RXRG、TFG、TP53
rno00620 / Pyruvate metabolism / LDHA、MDH1、PCK1、PDHB
rno00790 / Folate biosynthesis / GGH
rno04723 / Retrograde endocannabinoid signaling / ADCY3、ADCY5、GABRB1、GNAI2、GRIA3、ITPR1、MGLL、RIMS1、SLC17A6、SLC32A1
rno00400 / Phenylalanine, tyrosine and tryptophan biosynthesis / PAH
rno00330 / Arginine and proline metabolism / ARG1、CNDP2、GLUD1、OAT、OTC
rno04726 / Serotonergic synapse / ADCY5、GABRB1、GNAI2、ITPR1、MAP2K1、PLA2G4A、RAF1、SLC18A2、TPH2
rno04728 / Dopaminergic synapse / ADCY5、CALM1、GNAI2、GRIA3、ITPR1、PPP1CB、SLC18A2
rno04270 / Vascular smooth muscle contraction / ADCY3、ADCY5、ADORA2B、CALM1、GNA12、ITPR1、MAP2K1、PLA2G4A、PPP1CB、PRKCD、RAF1
rno00630 / Glyoxylate and dicarboxylate metabolism / CAT、MDH1

supplement Table6. Predicted pathways of target genes at 14d after microwave exposure.

Code / Definition / Genes
rno04146 / Peroxisome / EHHADH、HAO2
rno04721 / Synaptic vesicle cycle / ATP6V1C1、ATP6V1F、CLTA
rno04114 / Ocyte meiosis / IGF1R、PPP2CB、PPP2R1A、YWHAH
rno00020 / Citrate cycle (TCA cycle) / DLST
rno04730 / Long-term depression / GRID2、IGF1R、PPP2CB、PPP2R1A
rno00380 / Tryptophan metabolism / CYP1A2、EHHADH
rno04020 / Calcium signaling pathway / ADRB3、CHRM2、PLCE1
rno04722 / Neurotrophin signaling pathway / NTRK2、YWHAH
rno00790 / Folate biosynthesis / QDPR
rno00520 / Amino sugar and nucleotide sugar metabolism / GPI
rno00512 / Mucin type O-Glycan biosynthesis / GALNT5
rno05134 / Legionellosis / ARF1、IL18
rno03420 / Nucleotide excision repair / ERCC3
rno04350 / TGF-beta signaling pathway / PPP2CB、PPP2R1A、RPS6KB1
rno04710 / Circadian rhythm – mammal / NR1D1
rno04914 / Progesterone-mediated ocyte maturation / IGF1R
rno04666 / Fc gamma R-mediated phagocytosis / RPS6KB1
rno00400 / Phenylalanine, tyrosine and tryptophan biosynthesis / GOT2、PAH
rno00330 / Arginine and proline metabolism / GOT2
rno04728 / Dopaminergic synapse / PPP2CB、PPP2R1A、PPP2R2A
rno04270 / Vascular smooth muscle contraction / PRKCH
rno00630 / Glyoxylate and dicarboxylate metabolism / HAO2

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