Table S1: Strains, Plasmids, and Oligonucleotides Used in This Study

Table S1: Strains, plasmids, and oligonucleotides used in this study

Genotype or description / Source
Strains
E. coli
TG1
CC118λpir
P. aeruginosa
PAO1
PAO1DclpV2
PAO1DvgrG2a
PAO1DvgrG2b
PAO1DvgrG2aDvgrG2b
PAO1vgrG2aV5
PAO1DvgrG2b vgrG2aV5
PAO1vgrG2b6His
PAO1DvgrG2a vgrG2b6His
PAO1vgrG2bV5 / supE, hsdDR, thiD(lac-proAB), F' (traD36, proAB+, lacIq, lacZDM15))
(lpir) D (ara-leu), araD, DlacX74, galE, galK, phoA-20, thi-1, rpsE, rpoB, Arg(Am), recA1, Rfr (lpir)
Wild-type prototroph, cytotoxic and invasive
clpV2 deletion mutant
vgrG2a deletion mutant
vgrG2b deletion mutant
vgrG2a and vgrG2b deletion mutant
chromosomally encoded VgrG2aV5 translational fusion in PAO1
chromosomally encoded VgrG2aV5 translational fusion in PAO1DvgrG2b
chromosomally encoded VgrG2bHis6 translational fusion in PAO1
chromosomally encoded VgrG2bHis6 translational fusion in PAO1DvgrG2a
chromosomally encoded VgrG2bV5 translational fusion in PAO1 / Laboratory
Laboratory
This work
(11)
This work
This work
This work
This work
This work
This work
This work
This work
Plasmids
pRK2013
pHM12
pKNG101
pKNG101-vgrG2a
pKNG101-vgrG2b
pCR2.1
pTS31
pTS30
pSBC58
pTS33
pTS32
pSBC62
pHM49
p2aHA-Strep
p2aHA-Strep
pGFP HA-Strep
pSBC63 / Tra+, Mob+, ColE1, KmR
pET-Dest42 encompassing vgrG2a-v5his6
oriR6K, mobRK2, sacBR+, SmR (suicide vector)
suicide vector for vgrG2a deletion, SmR
suicide vector for vgrG2b deletion, SmR
TA cloning, lacZa, ColE1, f1 ori, ApR KmR
vgrG2aV5 in pCR2.1, ApR KmR
vgrG2b6His in pCR2.1, ApR KmR
vgrG2bV5 in pCR2.1, ApR KmR
suicide vector for vgrG2aV5 insertion, SmR
suicide vector for vgrG2bHis6 insertion, SmR
suicide vector for vgrG2bV5 insertion, SmR
pMM67–42 encompassing clpV2-v5his6
doxycycline inducible VgrG2a with Strep-HA fusion
doxycycline inducible VgrG2b with Strep-HA fusion
doxycycline inducible GFP with Strep-HA fusion
pJN105 encompassing vgrG2b-his6 under PBAD / Laboratory
This work
(12)
This work
This work
Invitrogen
This work
This work
This work
This work
This work
This work
(11)
This work
This work
(2)
This work
Oligonucleotides
vgrG2a deletion
5’UG2a
3’UG2a
5’DG2a
3’DG2a
vgrG2aext5’
vgrG2aext3’.
vgrG2b deletion
5’UG2b
3’UG2b
5’DG2b
3’DG2b
vgrG2bext5’
vgrG2bext3’.
PAO1vgrG2aV5 tagging
TSO53
TSO54
TSO55
TSO56
TSO57
TSO58
PAO1vgrG2b6His tagging
TSO59
TSO60
TSO61
TSO62
TSO63
TSO64
PAO1vgrG2bV5 tagging
SB0108
SBO109
SBO110
SBO112
SBO115
SBO116
VgrG2a or 2bHA-Strep
2a/2bHA-StrpUp
2bHA-StrpDown
2aHA-StrpDown
GFPHA-Strep
GS2913_eGFP_N5
GS2914_eGFP_N3 / 5’AAGCCGGTGGTCATCACCAAGGTCTT3’
5’TCATGTGTTTTGACGCATGGATCGTTC3’
5’AACACATGATGAGCAGCGAACCGCTCG3’

5’TCGTCATTCCACCATGCCTGGAGTTCC3’

5’ACGGTTCTTGTCTAGCACCGATGCCC3’
5’GCGCTCATAGCCGTGCAACTGCGTCA3’
5’TGACCTCCGGCGAGCG3’
5’TCAGTATCCTTGACGCATGGATCGTTCCTTG3’
5’ATGCGTCAAGGATACTGACCAATGAAATGCAAG3’
5’GTCGAGCAGCATCCAGTTGC3’
5’CGGCAACACCTATCAGGAAG3’
5’CTCTCCTTGCCGCTCCTC3’
5’TCACGTAGAATCGAGACCGAGGAGAGGGTTAGGGATAGGCTTACCTGTGTTGGACTCCGTGTCGCTGTTGTGACTGCCC3’
5’GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTGAGCAGCGAACCGCTCGAGCCGAATCAGGATGTGATC3’
5’GGTGACCGCCGGGCAGAAGGTGGTGATCAACG3’
5’CCACCATGCCTGGAGTTCCCGCCGGCTGACGATG3’
5’CCTTTCCCTGCTCCTCACCCTGAT3’
5’CCTATCCCTAACCCTCTCCTCGGT3’
5’TCAGTGATGGTGATGGTGATGGTATCCCGTTGGGAAGTTTTTCAGTACG3’
5’CATCACCATCACCATCACTGACCAATGAAATGCAAGACCTTGCTCATTGC3’
5’CGAAATCGCCCGCCTCCGCAG3’
5’CATGGCTTCTCTCCTTGCCGCTC3’
5’TGTTGGACTGGGCAGAGACGATCG3’
5’CATCACCATCACCATCAC3’
5’GATATGAAACACACGAAATCGCCCGCCTCCGC3’
5’TCACGTAGAATCGAGACCGAGGAGAGGGTTAGGGATAGGCTTACCGTATCCCGTTGGGAAGTTTTTCAGTACGCCAGC3’
5’GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTGACCAATGAAATGCAAGACCTTGCTCATTGCCTGCC3’
5’CATGGCTTCTCTCCTTGCCGCTCC3’
5’CAGGGTCTGTTCATTGCCGAAATGACCGCCGTCTGGCAGCACCA3’
5’GAAGACCTCGATCTCCCCGGCA3’
5’GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGCGTCAAAGGGACCTGAAATTC3’
5’GGGGACCACTTTGTACAAGAAAGCTGGGTTCAGTATCCCGTTGGGAAG3’
5’GGGGACCACTTTGTACAAGAAAGCTGGGTTCATGTGTTGGACTCCGTGTCGC3’
5’GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGTGAGCAAGGGCGAG3’
5’GGGGACCACTTTGTACAAGAAAGCTGGGTTTACTTGTACAGCTCGTC3’ / This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
This work
(2)
(2)