Evaluation of GEM Germplasm for Resistance to Aflatoxin Contamination

Evaluation of GEM Germplasm for Resistance to Aflatoxin Contamination

Paul Williams and Gary Windham, USDA-ARS Corn Host Plant Resistance Research Unit, Mississippi State, MS

Forty-five single crosses, three-way crosses, inbred lines, and other GEM selections were evaluated in a field test with three replications at Mississippi State in 2013. Two inbred lines, Mp313E and Mp719, and two single crosses, Mp313E × Mp494 and Mp313E × Mp717, from the USDA-ARS breeding program were included as resistant checks. B73 × Va35 was included as a susceptible check. Primary ears of the plants in each plot were inoculated seven days after mid-silk with an Aspergillus flavus spore suspension using the side-needle technique. The suspension was injected underneath the husks into the side of the ear with a tree-marking gun. At maturity, ears were hand harvested, dried, and shelled. Grain was ground and analyzed for aflatoxin concentration using the Vicam Aflatest procedure. The values for aflatoxin concentration were transformed [ln(y+1), where y = concentration of aflatoxin in a sample] before statistical analysis. Transformed means were compared by an LSD at P = 0.5. Geometric means were calculated by converting the transformed means to the original units of measurement.

GEM germplasm evaluated for aflatoxin accumulation at Mississippi State in 2013.

PEDIGREE / Aflatoxin (ng/g)
INVENTORY# / ln(y+1) / Geometric Mean
(NEI9004:S2809-B-061-B-B/GEMS-0016)-B / 12GEM03392 / 6.92 / 1013
CML413:N18-001-036-002-B / 12GEM03405 / 6.23 / 508
GEMS-0002 / 09GEM07796 / 6.02 / 409
H8431/((GEMS-0115/Mp717)/TZAR101) / 11GEM06393 / 5.98 / 395
SCROGP3:N2017-172-001-B-B-SIB / 09GEM07820 / 5.98 / 393
((87916W/Mp717)/4676A)-B / 11GEM06410 / 5.96 / 385
BG070404:D27S42-B-014-B-B-SIB / 12GEM03021 / 5.91 / 369
((LH82/KO679Y)/GEMN-0205)-B / 12GEM03397 / 5.87 / 354
KUI44:N99am99bj / 12GEM80081 / 5.77 / 320
((794/KO679Y)/NEI9004:S2809-B-061-B-B)-B / 12GEM03394 / 5.76 / 317
PUER5:N99bb99bq / 12GEM80037 / 5.76 / 316
GEMN-0164 / 07GEM02612 / 5.71 / 302
(((26e/GEMN-097)-SIB-SIB)/GEMN-0046)-SIB / 12GEM09123 / 5.56 / 258
(GEMS-0002/GEMS-0003)-B-B-062-B / 12GEM03060 / 5.51 / 247
PHJ40/((GEMS-0115/Mp717)/TZAR101) / 11GEM06404 / 5.49 / 241
CML341:S99ad99bg / 11GEM80039 / 5.49 / 241
Ki 14:S99ad99be / 11GEM80018 / 5.42 / 225
SANM126:N1299b-B-033-B-B-SIB / 12GEM03026 / 5.27 / 193
CML373:S99al43 / 11GEM80103 / 5.25 / 190
B73 x Va 35 / Susc. Check / 5.22 / 185
(CUBA164:S2012-459-001-B/GEMS-0002)-B-B-031-B-B-SIB / 11GEM06359 / 5.20 / 181
UR13085:S99g99u-B-025-B / 11GEM06074 / 5.15 / 172
PHJ40/(Mp717/KO679Y) / 11GEM06405 / 4.93 / 137
DK888:N11(95)-B-027-001-040-B-B-B / 08GEM04784 / 4.88 / 131
((LH61/KO679Y)/GEMN-0205)-B / 12GEM03396 / 4.83 / 124
(794/KO679Y)-B / 11GEM06419 / 4.82 / 123
794/((GEMS-0115/Mp717)/TZAR101) / 11GEM06397 / 4.79 / 119
(GEMS-0126/GEMS-0002)-B / 12GEM09089 / 4.74 / 114
(CUBA164:S2012-459-001-B/GEMS-0002)-B-B-011-B-B-SIB / 11GEM06358 / 4.65 / 103
PEDIGREE / Aflatoxin (ng/g)
INVENTORY# / ln(y+1) / Geometric Mean
(GEMN-0196/GEMN-0205)/ANTIG03:N1242-B-007-B / 12GEM03979 / 4.54 / 93
DK888:N11(95)-B-027-012-009-B-B-B / 08GEM04785 / 4.53 / 91
CML311:S99aj99be / 11GEM80017 / 4.51 / 90
KUI44:S99al99bl / 11GEM80051 / 4.31 / 73
((KO679Y/CUBA117:S15-101-001-B-B-B-B-B-B-B)/GEMS-0016)-B / 12GEM03393 / 4.26 / 70
(GEMS-0030/CUBA117:S15-101-001-B-B-B-B-B-B-B)-B / 12GEM09088 / 4.23 / 68
DKXL380:N11-B-010-017-B-B-B / 08GEM04769 / 4.14 / 62
DK888:N11(95)-B-022-001-026-B-B / Ames 31234 / 3.87 / 47
794/(Mp717/KO679Y) / 11GEM06398 / 3.84 / 45
(KO679Y/CUBA117:S15-101-001-B-B-B-B-B-B-B)-B / 11GEM06411 / 3.66 / 38
H8431/(Mp717/KO679Y) / 11GEM06396 / 3.59 / 35
DK888:N11(95)-B-003-001-001-B / 08GEM05120 / 3.25 / 25
DKXL370A:S11-B-028-001-B-B-Sib-B-B / 05GEM02813 / 3.10 / 21
DK888:N11-B-027-012-B-007-B / 11GEM00710 / 2.87 / 17
KO679Y / 11GEM06555 / 2.66 / 13
GEMS-0030/KO679Y / 12GEM04507 / 2.58 / 12
(GEMS-0002/GEMS-0003)-B-B-046-B / 12GEM03059 / 2.19 / 8
Mp 313E x Mp 494 / Res. Check / 1.86 / 5
Mp 313E / Res. Check / 1.56 / 4
Mp 719 / Res. Check / 1.16 / 2
Mp 313E x Mp 717 / Res. Check / 0.00 / 0
LSD (0.05) / 1.65

Plot means were transformed [ln(y+1), where y=aflatoxin concentration] before statistical analysis, geometric means were calculated by converting transformed means transformed means to original units of measure.

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