First Hand Investigations

i)  Maintaining a Balance

·  identify data sources, plan, choose

equipment or resources and

perform a first-hand investigation

to test the effect of:

– increased temperature (e.g. potato/liver catalase in water baths etc.)

– change in pH (e.g. potato, catalase, different amounts of acid or base added, keep volumes the same)

-  substrate concentrations on the activity

of named enzyme(s) (e.g. liver,catalase, different amounts of peroxide and water, same volumes, same diameter tubes)

·  perform a first-hand investigation

to demonstrate the effect of

dissolved carbon dioxide on the

pH of water(pH meter, bubbled into water, pH went down)

·  perform a first-hand investigation

using the light microscope and

prepared slides to gather

information to estimate the size

of red and white blood cells and

draw scaled diagrams of each (measure diameter of field of view in mm with a ruler, multiply by 1000 to get micrometres, divide by number of cells going across slide- rbcaround 7 micrometres, wbc around 12-16 micrometres)

·  choose equipment or resources to

perform a first-hand investigation

to gather first-hand data to draw

transverse and longitudinal

sections of phloem and xylem tissue

·  perform a first-hand investigation of

the structure of a mammalian kidney

by dissection, use of a model or

visual resource and identify the

regions involved in the excretion of

waste products(know where cortex, medulla, pelvis, ureter, urethra are and what part of nephron is in each)

·  perform a first-hand investigation to

gather information about structures

in plants that assist in the

conservation of water (e.g. fleshy leaves in pigface, succulents, hairy coverings, waxy coatings, needle like leaves in some natives)

ii)  Blueprint of Life

·  plan, choose equipment or

resources and perform a first-hand

investigation to model natural

selection (green and brown toothpicks thrown on grass; the environment/green background is the selecting agent- more brown toothpicks were ‘preyed’ upon because they were easier to spot by the predators (us).)

·  perform a first-hand investigation

or gather information from

secondary sources (including

photographs/ diagrams/models)u to

observe, analyse and compare the

structure of a range of vertebrate

forelimbs (here they are; suggest a common ancestor)

·  perform an investigation to

construct pedigrees or family trees,

trace the inheritance of selected

characteristics and discuss their

current use (you did this in your research task, and wrote questions on them)

·  identify data sources and perform a

first-hand investigation to

demonstrate the effect of

environment on phenotype ( we investigated the effect of fertiliser on growth of shallots – remember 3-5 plants per pot, different amounts of fertiliser in each pot, dependent variable is height of plants)

·  perform a first-hand

investigation or process

information from secondary

sources to develop a simple

model for polypeptide synthesis (remember the DNA code was on the board, you drew the corresponding mRNA code and took it to the ribosome at the side of the room to get the corresponding tRNAs and their amino acids. This was in the prac. Test, too)

iii)  Search for Better Health

·  identify data sources, plan and

choose equipment or resources to

perform a first-hand investigation to

identify microbes in food or in water (we inoculated agar plates with pond water, fresh water etc., sealed them and incubated them at around 30 degrees for a few days. We counted the number and type of each colony – bacteria are shiny, fungi are furry etc.-nb sterile techniques)

·  perform an investigation to model

Pasteur’s experiment to identify

the role of microbes in decay (swan neck flasks and open neck flasks; boil broth to kill existing microbes and leave to stand. Swan necked flasks did not decay nb: safety considerations)

·  identify data sources, plan and

perform a first-hand investigation

or gather information from

secondary sources to analyse and

present information about the

occurrence, symptoms, cause,

treatment/management of a named

non-infectious disease (really a secondary source exercise- examples: Down syndrome, scurvy, lead poisoning etc.))

·  perform an investigation to

examine plant shoots and leaves

and gather first-hand information

of evidence of pathogens and insect

pests (e.g. powdery mildew (a fungus) on Impatiens, Crepe Myrtle, Lacebug(an insect) on azalea leaves, smut and rust(fungi) on wheat, aphids (insects) on roses)