1ACATGGGGGAATTTAAAGGTCTCTGTACGTCTAAAAGGGAAGACCCTGGAGAGGATTAGATAGTAC
ACATTTTCCTTGTAACGTGAATTCACTTTTCAATTTTCACCCCACGTGAATCATAGGACCAAA
130 ATGTCAAAGCTGCCATCCAATCGCCATCTACCTCAGCCCTCCAGACTGAGGCCTCCTGGA
M S K L P S N R H L P Q P S R L R P P G
190 TCAGCCATGAAGCGTCTGGGGAGTGACAGTGCTATAACTTCCCCTGAGAAGCGGTCCAGG
S A M K R L G S D S A I T S P E K R S R
250CATTCTGGAGATGCTGAAGACGCTGGTATCATGTCAAGGCCTCAGTACCGTGGAGGCACC
K S G D A E D A G I M S R P Q Y R G G T
310 AGGGGAGGACGTGGCAGTCTCAGCAGATCTGTGTCCATGGCCAAC CTAAAC TCT GGTGTT
R G G R G S L S R S V S M A N L N S G V
370 ACAAGAAATAGAGGCATAACCAAGAGGAACTTGGCCAGCTCCAGG ATG GGT GCATCAACC
T R N R G I T K R N L A S S R M G A S T
430 CTCAAC CTGACTGGT CGTCCGTCT AACATC ACT GCT GCT TCG GCCAACCTTGTCCGA CAA
L N L T G R P S N I T A A S A N L V R Q
490 GGA GCA GTG TCT CGT AGG CCT GGG GCA AAC ATC ACCAACCGAGTAAACAACACCCAGGAC
G A V S R R P G A N I T N R V N N T Q D
550 ACTGACACAGGTCCTGGAGGGGATGCAGTCACTAAAGCCAAGAAGAAAAGGGCTCCATGG
T D T G P G G D A V T K A K K K R A P W
610 GATCTGAAAGGACGACTACAAGACATGGAGGAACTGGTCAAGTGTAGCACAGTTGAGCGG
D L K G R L Q D M E E L V K C S T V E R
670 GAAAACCTGCTGGCCAAGTTCAATGATTATGACAGTCGCATTCAAAATCTGGAAGAGGAA
E N L L A K F N D Y D S R I Q N L E E E
730 AAACAAAACTTGAACCAAAACTTGCAACAGTCTCATTCCACAACTCAAGCCAATCAGGAA
K Q N L N Q N L Q Q S H S T T Q A N Q E
790 CAGATTGATGCTCTGACTACCAAGCTCAATGAAGAAGTAAGGCAGAGAAGTAATGAAAGA
Q I D A L T T K L N E E V R Q R S N E R
850 CAAGTATTGGAAAGAAACATTGAAGAACTGCAATCCAAGAAATCTTCCCTTGAAAGGAGG
Q V L E R N I E E L Q S K K S S L E R R
910 ATCAAGTCACTGGAAGCAGATCTCTCATCCAGGGAAGATGAGCTGGCAGGTCTTAAGACC
I K S L E A D L S S R E D E L A G L K T
970 ACAGTTTCTAACTTGACAAGTGTCTCAAGTGGCCTTGAGGCTCAACTGAGCTCTACAAAA
T V S N L T S V S S G L E A Q L S S T K
1030 CTACTACTGAATGACCGGAATAAGAAGGTGGCTGAATTAGAAGATGAAACTTCTACTCAA
L L L N D R N K K V A E L E D E T S T Q
1090 GCTAATACTATTATTATCCTTGAAAAGAAGTTGTTGGAGGGTGAGACTTTCCGTAGGAAG
A N T I I I L E K K L L E G E T F R R K
1150 CTTCATAACCAAGTCTTGGAGTTAAAGGGAAACATTAGAGTTTTCTGTCGGGTACGTCCT
L H N Q V L E L K G N I R V F C R V R P
1210 CTGATAGGAGAGGAAATCAAGAACAACGGGGATTCCAGTGATATCCATCACATTAATTTT
L I G E E I K N N G D S S D I H H I N F
1270 CTTGATGAACGCACTCTTGAGCTTTGTAAAGGTGGAGATGTGAAC AGCAGCACTATGTCT
L D E R T L E L C K G G D V N S S T M S
1330 GGCCTCAAGGGGCGAGGAAACGGTCTCTTGGAATTCACGTATGACAGGGTGTTCACCCCG
G L K G R G N G L L E F T Y D R V F T P
1390 TGTGCAACCCAAGCAGAAGTATTTGAGGAGATATCTCAGCTGGTCCAGTCAGCTCTGGAT
C A T Q A E V F E E I S Q L V Q S A L D
1450 GGATATAATGTGTGTGTGTTTGCTTATGGTCAGACTGGATCAGGCAAGACTTTCACCATG
G Y N V C V F A Y G Q T G S G K T F T M
1510 GAAGGCTGCCCTAATAGTGAGGAACTGGAAGGTGTCATCACCAGAACAGTGAGACATGTG
E G C P N S E E L E G V I T R T V R H V
1570 TTTACTTCAATGAAGGAACTGAAAGATAAGGGTTGGACGTACACAGTTGAGGCCAGCTTC
F T S M K E L K D K G W T Y T V E A S F
1630 CTGGAAATCTATAATGAGACCATCAGAGACCTGCTGGTGTCATCAAAAGAGGCCAAGAAT
L E I Y N E T I R D L L V S S K E A K N
1690 CTGACATATGACATAAAATTGGTGGACAACAAAAAGAGCGATACCTATGTCACAAATCTT
L T Y D I K L V D N K K S D T Y V T N L
1750 AAGATTGTTCCTGTGACAGATGAAACCCAGGTGCACCAGCTGCTGAGGATGGCACAACAG
K I V P V T D E T Q V H Q L L R M A Q Q
1810 CAGCGTGCAGTGGCAGCTACCAACATGAATGAACGCTCCTCTCGGTCTCATTCAGTCTTC
Q R A V A A T N M N E R S S R S H S V F
1870 CGCCCTAAACTTGTGGGAGAGAATTCTAGGACCTCTGAGTCTTGTGAAGGAACCCTGAAC
R P K L V G E N S R T S E S C E G T L N
1930 CTGGTGGACTTGGCTGTGTCAGAGCGACTTAAGGAATCTGGTTCAGAAGGTGCCAGGCTT
L V D L A V S E R L K E S G S E G A R L
1990 ACCGAGACTCAAAATATCAACCGGTCCTTGTCTAATCTGGGCAATGTCATCATGGCTCTT
T E T Q N I N R S L S N L G N V I M A L
2050 GCACAGAAGCAAGGACACATTCCTTACCGCAACTCCAAGCTGACCCATCTTCTGCAAAAC
A Q K Q G H I P Y R N S K L T H L L Q N
2110 TCCCTTGGTGGCAACTCAAAGACCCTGATGTTTGTGAATGTGTCTCCAGTAGACTTATGC
S L G G N S K T L M F V N V S P V D L C
2170 TTTAGTGAAACTCTTAATTCCCTTCGATTTGCCACCAAAGTTAATCAGTGTCACATAGGT
F S E T L N S L R F A T K V N Q C H I G
2230 ACTGCCATAAAACAGGTCAAGAAGTAAAGCCACCCTTATTTTGTATTTGGGCTTCTAGCT
T A I K Q V K K *
2290 GGATTTTGTCTTCTCAACCTGTCTTTCAAAAAATATATTGATTTAAGGTTTTACAATTGTATTTAGCAAT
2360 ATGTATGCATAGTCAATCACTGTTTCCTTATATATTTTAATATAACATTAGCAACTGATAACACTTTGTTCAGAA
2435 TCAAAGTGTATTATTTTCACATAGTTCTCATAAAGCACCACTCATAATTTTAAGGTAGATATATTTTATC
2505 ATGTATTTTTAACATGCTGTGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC
2575 TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGGAAATATAATTTTAACAAGTAAAAAAAAAAAA
Figure S1. Full-length cDNA of the kifc1 in P. trituberculatus. The deduced amino acid sequence is shown below the necleotide sequence. The 2638 bp full length of kifc1cDNA consists of 129 bp a 5’ untranslated region, a 385 bp 3’ untranslated region and a 2124 bp open reading frame encoding708 amino acids.