Table S1: Primer sequences for qPCR.

Putative gene name / 5'-Forward primer-3' / 5'-Reverse primer-3' / Product size (bp)
CTGF / 5’-AGACCTTATGAAGTGGACCTGGA -3’ / 5’-TTGATGGGCTTGGAGATTTTG -3’ / 83
THBS1 / 5CGACAACTTACACAACTTGAAAACG 3 / 5’-TTTTGCTTCCCTCCCAATGT -3’ / 97
UBC / 5’-CTCAATGGTGTCACTAGGCTCAA -3’ / 5’-GACTACAACATCCAGAAAGAATCCA -3’ / 129
ELOVL6 / 5’-CTACTTTGTGCTCTTCTGCCATTTT 3’ / 5’-TGGCTTCTGTCTCATTTCTACCAC -3’ / 99
FTH1 / 5’-CACACAGATGCGGGTCGTT -3’ / 5’-TGATGACTGGGAGAATGGACTG -3’ / 122
JGXG / 5’TTCCTATCACACAAGGATCGTGAG 3’ / 5’-GGAACTGCCAACAAGAAAATACATC -3’ / 130
ACSL5 / 5'-TGTGGCAGAAATGAACTAC-3' / 5'-TGCTGTCTTCTCAGGGTC-3' / 103
APOVLDL / 5'-AGTCCTGGTCCCAACAAC-3' / 5'-GTGAACAAGATGTCCCCTA-3' / 78
CIDEA / 5'-TGTGAAGGCAACTTTCTATG-3' / 5'-TGTCCAGTTATTTGTGCC-3' / 120
MTP / 5'-GCTCTTCCTCTGCCTACTCTG-3' / 5'-TCCTTCATCGGGAGTTGC-3' / 212
PCK / 5'-GAATCGTGAGTTGGGATGAGC-3' / 5'-GCGGTGTCTATTGGGAAGG-3' / 122
SCD-1 / 5'-GCCATCGGTCCTACAAAGC-3' / 5'-AGCCAATGTGGGAGAAGAAA-3' / 180
PPARγ / 5'-ACTCCCTTATGGCTATTGAA-3' / 5'-GCCCTTACAGCCTTCACA-3' / 89
RPS4X / 5'-CGGCGACGAGGTGAAGAA-3' / 5'-CCCGCAGGGTAGGTGATGT-3' / 84
GSTS1 / 5'-CAGAGGACGGTCAGGAATG-3' / 5'-CTGTCTCCCTCGTGTCTGC-3' / 175
ALDH3A2 / 5'-AGAAGACCAGTCCCAAGC-3' / 5'-AGATGCCTAACAGCCAGA-3' / 87
ADH5 / 5'-ATGAGTCCTTTCCCTTGAGT-3' / 5'-GGATGTTTCCCTGTGATTTT-3' / 194
YBX1 / 5'-AGATAGTGGAGGGTGCTGACA-3' / 5'-TGGCTGCTGACTTTGGGT-3' / 182
ASAH1 / 5'-TAACTCGGGAACTGAAGC-3' / 5'-TAACTCGGGAACTGAAGC-3' / 222
*β-actin / 5’-CAACGAGCGGTTCAGGTGT -3’ / 5’-TGGAGTTGAAGGTGGTCTCGT -3’ / 92
*18S / 5'TTGGTGGAGCGATTTGTC3' / 5'ATCTCGGGTGGCTGAACG3' / 129

* Housekeeping gene for data normalization.

Table S2: Summary of the contents of the SSH-1 and SSH-2 cDNA libraries derived from Landes geese and Sichuan white geese hepatocytes.

*SSH-1 / **SSH-2 / Total
Clones sequenced / 65 / 68 / 133
Empty plasmids / 3 / 2 / 5
Sequences obtained / 62 / 66 / 128
Number of contigs / 24 / 30 / 54
Sequence length range (bp) / 201-793 / 211-799 / 201-799
Average sequence length (bp) / 357 / 415 / 389
Singleton ESTs / 7 / 13 / 20
Novel sequences / 4 / 4 / 8

*SSH-1: cDNA from Landes geese as the “tester” and the cDNA from Sichuan White geese as the “driver”;

**SSH-2: cDNA from Sichuan White geese as the “tester” and the cDNA from Landes geese as the “driver”.

Table S3: Identified sequences from the SSH-1 library.

GenBank ID. / Clone no. / Frequency / Blast identity / Blast database no. / Identity % / e value
Known genes
GO240727 / ASH-A002 / 2 / ubiquinol--cytochrome c reductase / XM_414356 / 91 / 4.00E-166
GO240729 / ASH-A021 / 6 / transglutaminase 3 (LOC419215) / XM_417392 / 95 / 1.00E-140
GO240730 / ASH-A034 / 3 / thrombospondin 1 (THBS1) / XM_421205 / 88 / 1.00E-92
GO240731 / ASH-A050 / 1 / small EDRK-rich factor 1 / XM_0012319 / 89 / 6.00E-67
GO240732 / ASH-A061 / 3 / dolichyl-phosphate mannosyltransferase / XM_417511 / 87 / 7.00E-81
GO240734 / ASH-A082 / 4 / microsomal triglyceride transfer protein / NM_001109784 / 92 / 2.00E-130
GO240735 / ASH-A098 / 2 / myotubularin-related protein 1 / XM_420356 / 88 / 2.00E-81
GO240736 / ASH-A109 / 3 / ribosomal protein S4, X-linked (RPS4X) / NM_205108 / 94 / 1.00E-84
GO240737 / ASH-A114 / 3 / connective tissue growth factor (CTGF) / NM_204274 / 94 / 0
GO240739 / ASH-A146 / 3 / ARFGAP2 / NM_001033871 / 90 / 1.00E-47
GO240740 / ASH-A157 / 1 / R3H domain and coiled-coil containing 1 / XM_417686 / 86 / 2.00E-19
GO240742 / ASH-A178 / 4 / cell death activator CIDE-A / XM_001231716 / 92 / 1.00E-86
GO240743 / ASH-A194 / 2 / microsomal glutathione S-transferase 2 / XM_001234229 / 92 / 1.00E-44
GO240744 / ASH-A210 / 1 / Lsm3 protein (LOC416040) / XM_414380 / 85 / 4.00E-20
GO240745 / ASH-A232 / 1 / heterogeneous nuclear ribonucleoprotein A3 / NM_001031253 / 96 / 3.00E-176
GO240746 / ASH-A242 / 3 / aldehyde dehydrogenase 3 family, member A2 / NM_001006223 / 89 / 2.00E-29
GO240747 / ASH-A258 / 5 / dehydrogenase/reductase (SDR family) member 7 / XM_421423.2 / 80 / 9.00E-44
GO240748 / ASH-A290 / 2 / acyl-CoA synthetase long-chain family member 5 / NM_001031237 / 91 / 2.00E-118
GO240749 / ASH-A297 / 4 / phosphoenolpyruvate carboxykinase 1 / NM_205471 / 90 / 2.00E-112
GO240750 / ASH-A322 / 3 / peroxisome proliferator-activated receptor gamma / NM_001001460 / 92 / 3.00E-103
Unknown genes
GO240728 / ASH-A018 / 1 / 100 / 0
GO240733 / ASH-A066 / 3 / 100 / 0
GO240738 / ASH-A130 / 1 / 100 / 0
GO240741 / ASH-A162 / 1 / 100 / 0

Table S4: Identified sequences from the SSH-2 library.

GenBank ID. / Clone no. / Frequency / Blast identity / Blast database no. / Identity % / e value
Known genes
GO240751 / SAH-S004 / 4 / alcohol dehydrogenase 5 / NM_001031152 / 93 / 2.00E-161
GO240753 / SAH-S023 / 5 / PAPS synthase2 / XM_421557 / 91 / 4.00E-13
GO240754 / SAH-S025 / 1 / thiosulfate sulfurtransferase / XM_416284 / 73 / 2.00E-33
GO240755 / SAH-S029 / 4 / ELOVL family member 6, / NM_001031539 / 94 / 5.00E-137
GO240757 / SAH-S041 / 1 / stem cell antigen 2 (LOC420301) / XM_418413 / 88 / 7.00E-57
GO240758 / SAH-S057 / 4 / aldehyde dehydrogenase 1 family, / NM_204577 / 92 / 0
GO240759 / SAH-S074 / 2 / N-acylsphingosine amidohydrolase / NM_001006453 / 92 / 2.00E-130
GO240760 / SAH-S082 / 1 / hect domain and RLD5 / XM_420476 / 88 / 1.00E-14
GO240761 / SAH-S097 / 1 / Rps16 protein / XM_416113 / 95 / 2.00E-158
GO240762 / SAH-S099 / 2 / MMD / NM_001030704 / 84 / 8.00E-19
GO240763 / SAH-S113 / 3 / neuroepithelial cell transforming gene 1 / NM_001030648 / 100 / 4.00E-25
GO240764 / SAH-S115 / 1 / CD80 molecule / NM_001079739 / 81 / 8.00E-23
GO240765 / SAH-S119 / 2 / ysteine and glycine-rich protein 2 / NM_205208 / 93 / 0
GO240766 / SAH-S124 / 3 / ferritin, heavy polypeptide 1 (FTH1), / NM_205086 / 94 / 3.00E-135
GO240767 / SAH-S129 / 2 / chaperonin containing TCP1 / NM_001006216 / 93 / 0
GO240768 / SAH-S138 / 1 / transglutaminase 3 / XM_417392 / 89 / 1.00E-97
GO240770 / SAH-S158 / 2 / actin, beta (ACTB) / NM_205518 / 91 / 6.00E-106
GO240771 / SAH-S161 / 1 / peptidoglycan-binding,domain containing 2 / XM_001232775 / 90 / 3.00E-73
GO240772 / SAH-S179 / 1 / Y box binding protein 1 / NM_204414 / 98 / 2.00E-83
GO240773 / SAH-S187 / 4 / ubiquitin C / NM_001079474 / 93 / 4.00E-166
GO240774 / SAH-S195 / 1 / eukaryotic translation initiation factor 1 / XM_418159 / 99 / 3.00E-138
GO240775 / SAH-S213 / 4 / electron-transfer-flavoprotein, alpha / NM_001030543 / 91 / 2.00E-124
GO240776 / SAH-S221 / 1 / otokeratin (LOC395772) / NM_204932 / 88 / 2.00E-65
GO240777 / SAH-S239 / 1 / proteasome (prosome, macropain) subunit, / NM_001012860 / 93 / 1.00E-132
GO240778 / SAH-S247 / 3 / stearoyl-CoA desaturase / NM_204890 / 92 / 0
GO240780 / SAH-S297 / 4 / lipoprotein (APOVLDLII) / NM_205483 / 85 / 7.00E-59
Unknown genes
GO240752 / SAH-S009 / 1 / 100 / 0
GO240756 / SAH-S039 / 2 / 100 / 0
GO240769 / SAH-S145 / 1 / 100 / 0
GO240779 / SAH-S289 / 3 / 100 / 0