Table S1. Chromosome-wide average open:input chromatin ratios and correlations to other genomic features

Chromosome / Genes/Mb / % GC / open:input / Genomic DNA:input / Replication time / Expression level / Expression probability
19 / 24 / 49 / 2.23 / 1.04 / 1.72 / 132.2 / 0.45
17 / 16 / 45 / 1.75 / 1.19 / 1.64 / 61.2 / 0.33
22 / 15 / 48 / 1.93 / 1.15 / 1.75 / 74.9 / 0.36
16 / 13 / 44 / 1.52 / 1.10 / 1.56 / 63.4 / 0.46
11 / 11 / 42 / 1.21 / 1.12 / 1.49 / 98.0 / 0.48
20 / 10 / 44 / 1.51 / 1.19 / 1.60 / 51.2 / 0.45
1 / 10 / 42 / 1.26 / 1.18 / 1.52 / 68.2 / 0.43
15 / 9 / 42 / 1.26 / 1.15 / 1.57 / 114.0 / 0.50
21 / 8 / 41 / 1.26 / 1.19 / 1.42 / 27.2 / 0.43
12 / 8 / 41 / 1.14 / 1.09 / 1.50 / 76.8 / 0.45
14 / 8 / 41 / 1.14 / 1.07 / 1.46 / 65.8 / 0.46
7 / 8 / 40 / 1.15 / 1.08 / 1.45 / 110.3 / 0.41
9 / 8 / 41 / 1.02 / 0.99 / 1.44 / 99.7 / 0.44
6 / 7 / 40 / 1.01 / 1.06 / 1.44 / 58.8 / 0.34
10 / 7 / 42 / 1.09 / 1.00 / 1.49 / 47.6 / 0.38
X / 7 / 39 / 0.88 / 1.56* / 1.38 / 49.1 / 0.32
3 / 6 / 40 / 0.97 / 1.03 / 1.43 / 86.8 / 0.51
2 / 6 / 40 / 0.99 / 1.11 / 1.43 / 121.8 / 0.50
Y / 6 / 39 / 0.99 / 0.35* / 1.32 / 15.4 / 0.25
5 / 5 / 40 / 1.09 / 1.09 / 1.42 / 44.1 / 0.41
8 / 5 / 40 / 1.07 / 1.09 / 1.39 / 85.8 / 0.39
4 / 5 / 38 / 0.94 / 1.06 / 1.34 / 36.4 / 0.32
18 / 4 / 40 / 1.07 / 1.16 / 1.42 / 57.6 / 0.49
13 / 4 / 38 / 0.91 / 1.00 / 1.36 / 92.8 / 0.45

The ratio of open:input chromatin was calculated from the average of two independent fractionations of open chromatin, each hybridised twice with colour reversal to the 1Mb microarray. These values were averaged across each chromosome. Genomic DNA (female):input chromatin (male) hybridisation ratios per chromosome demonstrate that there is not preferential release of chromatin from gene-rich chromosomes, since ratios for autosomes approximate to 1, compared with the sex chromosomes (*). Gene density per chromosome was calculated from ENSEMBL, and the GC content of each chromosome was taken from Venter et al. (2001). The mean replication time for each chromosome was determined using the same whole genome microarray (Woodfine et al., 2004). Gene expression data for lymphoblasts (Woodfine et al., 2004) was expressed both as average expression level (arbitrary units) or probability of expression for all genes assayed per chromosome.