Supplementary figure legends, figuresand tables
S-Fig. 1Profile of 1kb DNA Marker#SM0331 (Fermentas,USA) (lane 1) and the dsRNA extracted from the mycelia (lane2) in 1% agarose gel electrophoresis.
S-Fig. 2The electrophoretic profile of total dsRNAs from virions in 1.5% agarose gel (lane 1)and the corresponding Northern hybridization results (lane 2,3 and 4). The [32P]dCTP-labeling probes were prepared from the cDNAs of the corresponding dsRNA segments, and the total dsRNAs were transferred onto Hybond-N+ membrane in parallel and was used as the targets.
S-Fig. 1
S-Fig. 2
Supplementary-Table 1 Comparison of the 5′ and 3′ terminal sequences of AoR1 and AoR2 with those contained in Partitiviruses, such as P. stoloniferumvirusS (PsV-S), G.abietinaRNA virus MS1 (GaRV-MS1), G. abietinaRNA virus MS2 (GaRV-MS2), Discula destructivavirus 1 (DdV1) and D.destructivavirus 2 (DdV2). Identical nucleotides with those of AoR1 and AoR2 were marked in bold and with grayish background color.
Segments / 5′ / Terminal sequences / Terminal sequences / 3′AoR1[EU118277] / 5′ / CGCAAAACU / UAAAUAACUCC / 3′
AoR2[EU118278] / 5′ / CGCAAAACU / GUCAUAUCUCC / 3′
PsV-S S1 [AM040148] / 5′ / CGCAAAAUC / AAUAAAUCUCU / 3′
PsV-S S2 [AM040149] / 5′ / CGCAAAAUU / AGUAAAUCUCU / 3′
GaRV-MS1 dsRNA1[AY089993] / 5′ / CGCAAAAUA / AUUAACAAUCA / 3′
GaRV-MS1 dsRNA2 [AY089994] / 5′ / CGCAAAAUU / AACCAACCUCU / 3′
GaRV-MS2 dsRNA1[AY615211] / 5′ / CGCAAAAUA / AUUAACAAUCA / 3′
GaRV-MS2 dsRNA2 [AY615212] / 5′ / CGCAAAAUU / AACCAACCUCU / 3′
DdV1 dsRNA1[AF316992] / 5′ / GCGCAAAAG / GUAAAAACUCC / 3′
DdV1 dsRNA2 [AF316993] / 5′ / CGCAAAAUU / CAUAAACCUCC / 3′
DdV2 dsRNA1 [AY033436] / 5′ / GCGCAAAAC / AGUAAAACUCA / 3′
DdV2 dsRNA2 [AY033437] / 5′ / GCGCAAAAG / CAUAAAACUCC / 3′
Supplementary-Table 2Theidentityamong AoR2 putative CPand those of genus Partitivirus, PsV-S,GaRV-MS1, GaRV-MS2, Botryotinia fuckeliana partitivirus 1(BfPV1) (unpublished),DdV2, DdV1,Ophiostoma partitivirus1(OPV1).
Partitivirus / IdentityPsV-S [YP_052857] / 63%
GaRV-MS1 [NP_659028] / 56%
GaRV-MS2 [YP_138541] / 56%
BfPV1 [CAM33267] / 58%
DdV2 [NP_620302] / 57%
DdV1 [NP_116742] / 50%
OPV1 [CAJ31887] / 50%