RODENT ENDPOINT PCR/RT-PCR FORM (D-1-M)
Please print and complete this form and forward (via e-mail or fax to MDS/RRDA. We will contact you for a detailed quotation.
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Mouse Panels:
18 Tests Panel
1. Mycoplasma pulmonis
2. Sendai Virus
3. Mouse Hepatitis Virus
4. Pneumonia Virus of Mice
5. Minute Virus of Mice
6. Mouse Parvovirus
7. Theiler's GD-VII
8. Reo-3 Virus
9. Mouse rotavirus
10. Ectromelia (Poxvirus)
11. Lymphocytic Choriomeningitis Virus
12. Polyoma virus
13. K Virus
14. Mouse Adenovirus
15. Mouse cytomegalovirus
16. Lactodehydrogenase Virus
17. Choice of Mouse Thymic Virus or Mouse Parvovirus (MMV)
18. Choice of Hantaan virus or other Mycoplasma spp. *
15 Tests Panel
1. Mycoplasma pulmonis
2. Sendai Virus
3. Mouse Hepatitis Virus
4. Pneumonia Virus of Mice
5. Minute Virus of Mice
6. Mouse Parvovirus
7. Theiler's GD-VII
8. Reo-3 Virus
9. Mouse rotavirus
10. Ectromelia (Poxvirus)
11. Lymphocytic Choriomeningitis Virus
12. Polyoma virus
13. K Virus
14. Mouse Adenovirus
15. Mouse cytomegalovirus
13 Tests Panel
1. Mycoplasma pulmonis
2. Sendai Virus
3. Mouse Hepatitis Virus
4. Pneumonia Virus of Mice
5. Minute Virus of Mice
6. Mouse Parvovirus
7. Theiler's GD-VII
8. Reo-3 Virus
9. Mouse rotavirus
10. Ectromelia (Poxvirus)
11. Lymphocytic Choriomeningitis Virus
12. Polyoma virus
13. K Virus
Rat Panels:
15 Tests Panel
1. Mycoplasma spp.
2. Pneumonia virus of mice
3. Kilham’s rat virus
4. Toolan’s H1 virus
5. Rat Parvovirus
6. Lymphocytic Choriomeningitis Virus
7. Rat cytomegalovirus
8. Sendai Virus
9. Rat coronavirus
10. Sialodacryoadenitis virus
11. Rat minute virus
12. Seoul virus
13. Mouse Adenovirus
14. Reovirus 3
15. Theiler’s murine encephalomyelitis virus
11 Tests Panel
1. Mycoplasma spp.
2. Pneumonia virus of mice
3. Kilham’s rat virus
4. Toolan’s H1 virus
5. Rat Parvovirus
6. Lymphocytic Choriomeningitis Virus
7. Rat cytomegalovirus
8. Sendai Virus
9. Rat coronavirus
10. Sialodacryoadenitis virus
11. Rat minute virus
Hamster Panel:
11 Tests Panel
1. Mycoplasma spp.
2. Pneumonia virus of mice
3. Kilham’s rat virus
4. Toolan’s H1 virus
5. Hamster Parvovirus
6. Lymphocytic Choriomeningitis Virus
7. Mice minute virus
8. Theiler’s murine encephalomyelitis virus
9. Sendai virus
10. Reovirus 3
11. Hantaan virus
Other animal pathogens
Rotavirus, equine Rotavirus, feline Rotavirus, canine
Rotavirus, bovine Rotavirus, porcine Equine influenza virus
Equine protozoal myeloencephalitis (Sarcocystis neurona)
Samples (For multiple samples, please complete Form D-3.)
What type of sample will be submitted? (see recommendations page 3)
Sample source: human rat mouse other
Cell culture (flask size; number of flasks ; passage # )
Cell pellet (number of cells ; lot # )
Tissue (type ; amount )
DNA (concentration ; volume ; total amount ng)
RNA (concentration ; volume ; total amount ng)
Lysis buffer (if applicable)
Special components of buffer (if applicable)
Recommended Sample Size for Endpoint PCR
Sample
/ AmountCultured cells / 5 x 10^6
Liver / 25 mg (~2mm3)
Brain / 25 mg
Lung / 25 mg
Heart/muscle / 25 mg
Kidney / 25 mg
Spleen / 10 mg
Plant leaf / 100 mg
Mouse tail / 0.8-1.2 cm
Blood / 1 ml
Buffy coat / 50 ul
DNA / 50 ng/reaction
RNA / 100 ng RNA/reaction
Other / Call
Recommended Shipping Conditions
Plasmid DNA / Liquid = A, W, D; pellet APCR products / Liquid = A, W, D; pellet A
Primers / Liquid = A, W, D; pellet A
cDNA or genomic libraries / With DMSO = D; without DMSO = W
RNA / D
Bacterial colonies / A, W
Stab culture / A
Cell banks / D
Glycerol stocks / D
Tissues / D
Blood / W
A = ambient temperature W = wet ice D = dry ice