Table S2. List of Most Significantly Differentially Regulated Genes Selected from Anns

Table S2. List of most significantly differentially regulated genes selected from ANNs for the determination of sex in dolphins from 4 geographic locations.

(Blastx cutoff value 1.0E-3).

EST ncbi Acc.no. / Seq. Description / Seq. Length / #Hits / min. eValue / mean Similarity / #GOs
Contig23 (DV468056, DV468606) / beta-2-microglobulin precursor / 615 / 20 / 3.113E-50 / 0.8995 / 5
Contig8 (DT661121, DT661130) / endonuclease reverse transcriptase / 757 / 20 / 9.245E-25 / 0.5075 / 0
Contig9 (DT661146, DV468349) / endonuclease reverse transcriptase / 655 / 20 / 6.793E-41 / 0.8995 / 0
Contig16 (DV467822, DV468030, DT661250) / endonuclease reverse transcriptase / 1021 / 20 / 1.295E-54 / 0.414 / 0
Contig17 (DV467848, DV468052, DT660342, DT660214) / eukaryotic translation elongation factor 1 alpha 1 / 1128 / 20 / 2.87E-147 / 0.99 / 8
Contig13 (DT661332, DV468062) / eukaryotic translation elongation factor 1 epsilon 1 / 801 / 20 / 5.033E-72 / 0.963 / 11
Contig18 (DV467901, DV468515) / h+ lysosomal v0 subunit e1 / 798 / 20 / 3.158E-42 / 0.924 / 9
Contig21 (DV467972, DV468548) / heat shock factor binding protein 1 / 536 / 20 / 3.485E-21 / 0.958 / 6
Contig27 (DV468285, DV468404) / histone / 751 / 20 / 2.354E-75 / 0.9865 / 10
Contig30 (DV468523, DV468543) / line-1 reverse transcriptase homolog / 622 / 20 / 5.259E-15 / 0.56 / 3
Contig15 (DV467784, DT661404) / nucleophosmin 1 isoform 1 / 628 / 20 / 7.359E-79 / 0.9915 / 2
Contig19 (DV467909, DT661152) / prothymosin alpha / 820 / 2 / 4.944E-14 / 0.79 / 0
Contig12 (DT661197, DT661410) / ribosomal protein l27 / 492 / 20 / 6.929E-65 / 0.987 / 4
Contig3 (DT660346, DV467881, DV468107) / ribosomal protein s3a / 800 / 20 / 6.84E-138 / 0.985 / 8
Contig22 (DV467994, DT660290) / selenoprotein k / 703 / 20 / 3.828E-19 / 0.934 / 2
Contig5 (DT660891, DT661019, DV468123, DT661205) / thymosin beta 4 / 622 / 19 / 1.228E-09 / 0.9347368 / 10
Contig14 (DT661382, DV468102, DT660273, DV468009, DV468341, DV467843, DV468605, DT660962, DV468076, DT660994, DT661003) / cytochrome c oxidase subunit iii / 1570 / 20 / 2.25E-122 / 0.938 / 4
Contig20 (DV467950, DT660370, DV467982, DV468000) / cytochrome c oxidase subunit i / 1154 / 20 / 1.76E-107 / 0.96 / 9
Contig25 (DV468145, DV467924, DV467772) / cytochrome c oxidase subunit i / 812 / 20 / 2E-116 / 0.935 / 9
Contig4 (DT660358, DV468456) / cytochrome c oxidase subunit ii / 577 / 20 / 3.64E-76 / 0.959 / 7
DT660143 / beta globin / 558 / 20 / 4.289E-47 / 0.9205 / 6
DT660146 / heavy polypeptide 1 / 534 / 20 / 8.524E-44 / 0.9825 / 8
DT660169 / c-c chemokine receptor-like 2 / 458 / 20 / 2.73E-29 / 0.7425 / 4
DT660171 / ribosomal protein l36a / 219 / 20 / 2.73E-16 / 1 / 4
DT660178 / elongation factor 1-alpha / 711 / 20 / 7.493E-55 / 0.9335 / 5
DT660181 / endonuclease reverse transcriptase / 370 / 20 / 3.296E-38 / 0.8355 / 0
DT660202 / s100 calcium binding protein a8 / 385 / 12 / 1.121E-06 / 0.875 / 6
DT660206 / acyl- synthetase long-chain family member 5 / 769 / 20 / 3.082E-84 / 0.9075 / 9
DT660222 / endonuclease reverse transcriptase / 390 / 20 / 2.795E-50 / 0.8285 / 0
DT660226 / proteoglycan 1 precursor-like / 778 / 20 / 5.425E-60 / 0.748 / 10
DT660231 / slam family member 7 / 597 / 20 / 6.878E-72 / 0.576 / 1
DT660247 / hypothetical protein PC101070.00.0 / 622 / 1 / 2.832E-06 / 0.81 / 0
DT660265 / nop16 nucleolar protein homolog / 687 / 20 / 7.96E-83 / 0.813 / 1
DT660268 / endonuclease reverse transcriptase / 301 / 20 / 4.443E-12 / 0.6075 / 0
DT660276 / endonuclease reverse transcriptase / 463 / 20 / 2.895E-18 / 0.8695 / 0
DT660327 / wd repeat domain 12 / 173 / 20 / 5.35E-25 / 0.9345 / 3
DT660336 / hCG2038477 [Homo sapiens] / 411 / 1 / 2.938E-07 / 0.64 / 0
DT660347 / endonuclease reverse transcriptase / 507 / 20 / 1.088E-16 / 0.3095 / 0
DT660351 / endonuclease reverse transcriptase / 507 / 20 / 1.039E-35 / 0.479 / 0
DT660352 / wd repeat domain 26 / 808 / 20 / 6.734E-40 / 0.98 / 1
DT660353 / ribosomal protein l12 / 567 / 20 / 7.795E-44 / 0.9865 / 7
DT660379 / transposase / 824 / 20 / 3.676E-25 / 0.63 / 0
DT660794 / cytochrome c oxidase subunit viib / 466 / 20 / 5.781E-35 / 0.855 / 4
DT660808 / polymerase ii (dna directed) polypeptide h / 544 / 20 / 7.915E-40 / 0.9635 / 6
DT660825 / nadh dehydrogenase 1 alpha 2 / 416 / 20 / 1.94E-43 / 0.8825 / 5
DT660836 / beta lysosomal-like / 719 / 1 / 8.84E-11 / 0.57 / 0
DT660847 / breast cancer early isoform cra_g / 707 / 20 / 4.91E-115 / 0.9335 / 18
DT660851 / dead (asp-glu-ala-asp) box polypeptide 39 / 789 / 20 / 5.67E-129 / 0.9665 / 7
DT660857 / nicotinamide phosphoribosyltransferase / 725 / 20 / 3.81E-102 / 0.9755 / 13
DT660858 / endonuclease reverse transcriptase / 515 / 20 / 1.535E-14 / 0.6315 / 0
DT660860 / proteasome ( macropain) activator subunit 2 (pa28 beta) / 756 / 20 / 4.18E-118 / 0.952 / 5
DT660862 / endonuclease reverse transcriptase / 374 / 13 / 3.783E-10 / 0.7438462 / 10
DT660863 / guanylate binding protein 3 / 732 / 20 / 2E-114 / 0.9375 / 4
DT660880 / ribosomal protein l32 / 494 / 20 / 9.668E-67 / 0.974 / 5
DT660902 / endonuclease reverse transcriptase / 718 / 20 / 7.785E-08 / 0.344 / 0
DT660913 / p40 / 776 / 20 / 1.932E-25 / 0.5185 / 3
DT660931 / 14 kd / 510 / 1 / 8.802E-05 / 0.47 / 0
DT660972 / nkg2a / 672 / 20 / 2.128E-77 / 0.748 / 3
DT660985 / endonuclease reverse transcriptase / 261 / 20 / 6.62E-31 / 0.824 / 0
DT660987 / cytochrome c oxidase subunit vib polypeptide 1 / 457 / 20 / 3.792E-47 / 0.965 / 4
DT660988 / mitochondrial ribosomal protein l22 / 456 / 20 / 2.238E-23 / 0.81 / 4
DT661002 / peroxiredoxin 1 / 541 / 20 / 8.468E-87 / 0.9645 / 7
DT661013 / endonuclease reverse transcriptase / 659 / 20 / 3.287E-51 / 0.8735 / 0
DT661036 / microfibrillar-associated protein 1 / 248 / 20 / 1.897E-14 / 0.999 / 2
DT661042 / mitochondrial ribosomal protein s33 / 595 / 20 / 1.833E-41 / 0.9045 / 3
DT661064 / orf2 / 393 / 19 / 6.026E-11 / 0.6415789 / 2
DT661081 / endonuclease reverse transcriptase / 636 / 20 / 5.647E-13 / 0.2565 / 13
DT661112 / endonuclease reverse transcriptase / 715 / 20 / 3.148E-28 / 0.506 / 0
DT661142 / poly (adp-ribose) glycohydrolase / 762 / 20 / 8.76E-108 / 0.986 / 6
DT661165 / signal sequence alpha / 718 / 20 / 9.16E-117 / 0.984 / 7
DT661199 / yme1-like 1 ( cerevisiae) / 679 / 20 / 9.451E-73 / 0.9895 / 8
DT661260 / transposase [Lipotes vexillifer] / 609 / 2 / 3.389E-09 / 0.63 / 0
DT661289 / signal peptidase complex subunit 1 homolog ( cerevisiae) / 531 / 20 / 3.137E-54 / 0.9705 / 6
DT661361 / endonuclease reverse transcriptase / 784 / 20 / 1.04E-79 / 0.4285 / 0
DT661378 / endonuclease reverse transcriptase / 737 / 20 / 2.862E-83 / 0.383 / 0
DT661388 / beta-2-microglobulin precursor / 627 / 20 / 3.222E-50 / 0.8995 / 5
DT661394 / leucyl-trna synthetase / 804 / 20 / 4.494E-28 / 0.979 / 4
DT661395 / bub3 budding uninhibited by benzimidazoles 3 homolog / 473 / 20 / 1.549E-56 / 0.9695 / 5
DT661401 / atp h+ mitochondrial f0 subunit isoform 2 / 380 / 20 / 5.072E-07 / 0.9285 / 11
DV467742 / son dna binding isoform f / 380 / 20 / 2.482E-54 / 0.999 / 3
DV467751 / endonuclease reverse transcriptase / 406 / 20 / 2.604E-07 / 0.539 / 0
DV467752 / cytochrome b / 765 / 20 / 7.74E-104 / 0.96 / 6
DV467753 / endonuclease reverse transcriptase / 681 / 20 / 3.932E-26 / 0.57 / 0
DV467758 / endonuclease reverse transcriptase / 429 / 20 / 3.682E-58 / 0.88 / 0
DV467780 / nhp2 non-histone chromosome protein 2-like 1 ( cerevisiae) / 526 / 20 / 4.976E-49 / 0.9845 / 8
DV467805 / atp h+ mitochondrial f1 epsilon subunit / 322 / 17 / 9.929E-11 / 0.9 / 7
DV467813 / chromobox homolog 3 (hp1 gamma drosophila) / 269 / 20 / 7.284E-22 / 0.8605 / 20
DV467833 / nadh dehydrogenase 1 alpha subcomplex 4 / 257 / 20 / 5.657E-22 / 0.9215 / 5
DV467837 / splicing factor subunit 1 / 683 / 20 / 1.992E-70 / 1 / 7
DV467845 / ribosomal p0 / 564 / 20 / 7.841E-81 / 0.99 / 6
DV467863 / septin 7 / 774 / 20 / 5.09E-135 / 0.9875 / 10
DV467871 / protein kinase c inhibitor aswz variant 5 / 537 / 20 / 9.246E-62 / 0.974 / 6
DV467928 / calmodulin / 699 / 20 / 1.581E-41 / 0.99 / 13
DV467942 / tetratricopeptide repeat domain 13 / 675 / 20 / 1.466E-17 / 1 / 1
DV467957 / isoform cra_a / 662 / 20 / 1.325E-54 / 0.622 / 3
DV467962 / beta-2-microglobulin precursor / 649 / 20 / 1.862E-51 / 0.907 / 5
DV467963 / p40 / 788 / 20 / 4.217E-60 / 0.642 / 3
DV467978 / thymosin beta 4 / 614 / 20 / 5.859E-09 / 0.961 / 10
DV467985 / p150 / 662 / 20 / 3.473E-16 / 0.7235 / 11
DV467999 / ribosomal protein l14 / 636 / 20 / 2.651E-47 / 0.976 / 3
DV468014 / endonuclease reverse transcriptase / 541 / 20 / 2.013E-39 / 0.6725 / 0
DV468034 / cyclin i / 398 / 20 / 5.643E-14 / 0.9265 / 2
DV468041 / cell division cycle 42 / 785 / 20 / 6.495E-69 / 0.9965 / 22
DV468044 / ribosomal protein l9 / 425 / 20 / 7.466E-67 / 0.983 / 10
DV468051 / isoform cra_b / 557 / 20 / 1.767E-53 / 0.85 / 2
DV468053 / cornichon homolog 4 / 497 / 20 / 8.035E-61 / 0.712 / 4
DV468075 / eukaryotic translation elongation factor 1 epsilon 1 / 140 / 9 / 0.0003033 / 0.7755556 / 8
DV468085 / h2a histone member v / 638 / 20 / 4.988E-54 / 0.9995 / 5
DV468098 / nadh dehydrogenase subunit 1 / 873 / 20 / 2.36E-105 / 0.9495 / 4
DV468101 / guanine nucleotide exchange factor p532 / 416 / 19 / 6.518E-07 / 0.9936842 / 4
DV468104 / mediator complex subunit 4 / 259 / 20 / 8.676E-31 / 0.885 / 9
DV468115 / anaphase promoting complex subunit 13 / 748 / 20 / 1.026E-36 / 0.917 / 4
DV468152 / regulator of g-protein signalling 1 / 383 / 20 / 5.36E-33 / 0.872 / 8
DV468171 / nuclear receptor binding set domain protein 1 / 323 / 20 / 3.638E-21 / 0.938 / 14
DV468180 / imap family member 7 / 391 / 20 / 1.591E-37 / 0.9015 / 1
DV468184 / ribosomal protein s25 / 455 / 20 / 9.085E-41 / 0.9915 / 5
DV468187 / endonuclease reverse transcriptase / 621 / 20 / 5.614E-63 / 0.8675 / 0
DV468206 / ribosomal protein s15a / 258 / 20 / 1.339E-31 / 0.9695 / 5
DV468217 / endonuclease reverse transcriptase / 413 / 20 / 4.38E-11 / 0.7275 / 0
DV468232 / p40 / 511 / 20 / 4.06E-26 / 0.7625 / 2
DV468239 / translocated promoter region (to activated met oncogene) / 595 / 20 / 5.151E-78 / 0.6235 / 8
DV468247 / nascent polypeptide-associated complex subunit alpha / 746 / 20 / 4.64E-98 / 0.9675 / 8
DV468268 / malate dehydrogenase nad / 283 / 20 / 8.745E-31 / 0.998 / 11
DV468297 / fucosyltransferase 8 / 564 / 20 / 1.378E-93 / 0.975 / 12
DV468298 / endonuclease reverse transcriptase / 359 / 20 / 2.172E-34 / 0.7715 / 0
DV468323 / c-type lectin-like protein / 553 / 20 / 2.428E-47 / 0.732 / 4
DV468336 / light chain smooth muscle and non-muscle / 421 / 20 / 1.81E-20 / 0.9795 / 7
DV468348 / endonuclease reverse transcriptase / 609 / 12 / 5.193E-10 / 0.5625 / 0
DV468360 / eukaryotic translation elongation factor 1 alpha 1 isoform 1 / 524 / 20 / 1.745E-22 / 0.98 / 5
DV468382 / dynein light chain roadblock-type 1 / 530 / 20 / 1.53E-45 / 0.951 / 7
DV468405 / light chain non-sarcomeric / 668 / 20 / 2.453E-86 / 0.9775 / 6
DV468406 / orf2 / 110 / 8 / 7.444E-06 / 0.82 / 3
DV468414 / casp8 and fadd-like apoptosis regulator precursor (cellular flice-like inhibitory protein) (c-flip) (caspase-eight-related protein) (caspase-like apoptosis regulatory protein) (mach-related inducer of toxicity) ( iso / 687 / 20 / 3.871E-37 / 0.86 / 4
DV468423 / centrin 3 / 625 / 20 / 1.753E-32 / 0.9365 / 5
DV468443 / ribosomal protein l23 / 505 / 20 / 1.708E-69 / 0.9755 / 5
DV468470 / p40 / 731 / 20 / 1.074E-43 / 0.671 / 3
DV468484 / ribosomal protein s11 / 429 / 20 / 7.01E-25 / 0.9895 / 6
DV468486 / endonuclease reverse transcriptase / 679 / 20 / 5.593E-53 / 0.6495 / 0
DV468492 / ribosomal protein s26 / 451 / 20 / 2.248E-39 / 0.996 / 5
DV468530 / ribosomal protein l38 / 328 / 20 / 1.017E-15 / 0.968 / 5
DV468569 / eukaryotic translation initiation factor 3 subunit k / 709 / 20 / 1.832E-45 / 0.954 / 7
DV468572 / endonuclease reverse transcriptase / 562 / 20 / 3.656E-06 / 0.7 / 18
DV468607 / transmembrane protein 184c / 743 / 20 / 4.267E-11 / 0.6875 / 2
DV468616 / alpha 1b / 630 / 20 / 3.699E-78 / 0.93 / 7
DV468626 / subfamily member 14 / 596 / 20 / 9.767E-27 / 0.777 / 9
DV799543 / toll-like receptor 7 / 434 / 20 / 5.372E-37 / 0.429 / 13
DV799549 / t cell receptor alpha chain / 279 / 10 / 0.0002321 / 0.74 / 0
DV799550 / signal transducer and activator of transcription 6 / 295 / 15 / 1.773E-44 / 0.8273333 / 12
DV799557 / aryl hydrocarbon receptor nuclear translocator / 424 / 20 / 5.482E-56 / 0.56 / 5
DV799558 / toll-like receptor 4 / 283 / 20 / 4.396E-29 / 0.55 / 1
DV799561 / toll-like receptor 2 / 245 / 20 / 2.567E-19 / 0.6885 / 8
DV799563 / protein kinase beta / 280 / 20 / 1.705E-31 / 1 / 23
DV799566 / interleukin 17a / 324 / 20 / 2.043E-40 / 0.812 / 8
DV799568 / interleukin 13 / 279 / 20 / 4.21E-22 / 0.906 / 9
DV799571 / cytochrome family subfamily polypeptide 1 / 338 / 20 / 2.54E-43 / 0.943 / 8
DV799573 / granulocyte-macrophage colony-stimulating factor / 388 / 17 / 7.214E-08 / 0.8817647 / 3
DV799577 / interleukin 2 / 287 / 20 / 5.12E-23 / 0.838 / 10
DV799583 / interferon gamma / 501 / 20 / 8.353E-37 / 0.935 / 19
DV799584 / interleukin-1 beta / 801 / 20 / 3.94E-109 / 0.8465 / 11
DV799590 / interleukin 1 alpha / 798 / 20 / 8.34E-144 / 0.8695 / 7
EG329066 / heat shock protein 70 / 523 / 20 / 1.116E-58 / 0.6495 / 17

ESTs selected from ANNs were assembled into contigs using CAP3 analysis. The resulting contigs and ESTs were then blasted against the (nr) non-redundant protein sequences database (nr) at NCBIusing Blast2go.