Electronic supplementary material

Figure 1: Damage score of pancreatic sections. The graph illustrates the mean score ± S.E.M. from 15 pancreatic sections in each group (n=4). ***P<0.001; significantly different from normal/control mice and ##P<0.01 significantly different from acute pancreatitis mice. Where, a: Normal control; b: Normal treatment with NDGA (30mg/kg, thrice); c: Acute Pancreatitis control; d: Acute Pancreatitis treatment with NDGA (30mg/kg/hr thrice).

Figure 2: HSP 27 expression as determined by western blot analysis. Results were normalized with respect to β-actin in respective control. All the values are represented as mean ± S.E.M. (n=3). **P<0.01 significantly different from normal/control mice and ##P<0.01 significantly different from acute pancreatitis mice. Where, a: Normal control; b: Normal treatment with NDGA (30mg/kg/hr for 3 hours); c: Acute Pancreatitis control; d: Acute Pancreatitis treatment with NDGA (30mg/kg/hr for 3 hours).

Figure 3: (A) NF-κB; (B) TNF-α; (C) p-p38; (D) Bcl-2; (E) p-PP2A and (F) procaspase-3 and caspase-3 expression were determined by western blot analysis in pancreas from different experimental groups. Results were normalized with respect to β-actin and total p38 in respective control. Similar results were obtained in three independent set of experiments. All the values are represented as mean ± S.E.M. (n=3). ***P<0.001, **P<0.01; significantly different from normal/control mice and ###P<0.001, ##P<0.01, #P<0.05; significantly different from acute pancreatitis mice. Where, a: Normal control; b: Normal treatment with NDGA (30mg/kg/hr for 3 hours); c: Acute Pancreatitis control; d: Acute Pancreatitis treatment with NDGA (30mg/kg/hr for 3 hours).

Figure 4: (A) Histone H3 acetylation, mono-methylation (K4 and K9) and H3 phosphorylation at Ser 10 and (B) H3 phosphorylation at Ser 28 were determined by western blot analysis of pancreas from different experimental groups. Results were normalized with total histone H3. Similar results were obtained in three independent set of experiments. All the values were represented as mean ± S.E.M. (n=3), **P<0.01; significantly different from normal/control mices and ##P<0.01 significantly different from acute pancreatits control. Where, a: Normal control; b: Normal treatment with NDGA (30mg/kg/hr for 3 hours); c: Acute Pancreatitis control; d: Acute Pancreatitis treatment with NDGA (30mg/kg/hr for 3 hours).