Supplementary Table S4 Experiment 1.2 Absolute call rates for 50-kHz call subtypes

CX / UR / DR / FL / SH / SP / SU / SD / MS / TR / FT / TJ / IU / CS / UC / MI
Saline
Median / 2.5 / 1.5 / 2 / 10.8 / 7.3 / 0.3 / 5 / 2 / 2 / 11.8 / 5.3 / 0.5 / 1.3 / 1.5 / 1.3 / 0
Mean / 6.7 / 5.3 / 2.8 / 25.5 / 10.6 / 1.1 / 12.6 / 3.2 / 4 / 26.3 / 17.8 / 2.1 / 3.5 / 3.6 / 3.1 / 0
Std. Error / 2 / 1.7 / 0.9 / 6.5 / 2.6 / 0.5 / 4.3 / 0.9 / 1.5 / 8.8 / 7.6 / 0.7 / 1 / 1.2 / 1.1 / 0
Minimum / 0.5 / 0 / 0 / 2.5 / 0.5 / 0 / 0 / 0.5 / 0 / 0 / 0 / 0 / 0.5 / 0 / 0 / 0
Maximum / 28 / 22 / 15.5 / 74 / 38.5 / 8.5 / 64.5 / 12 / 24 / 119 / 102 / 7.5 / 14.5 / 14.5 / 15.5 / 0
Morphine 0.3 mg/kg
Median / 5.5 / 2 / 2.8 / 13.5 / 7 / 0.8 / 8.8 / 4.8 / 2.8 / 18.8 / 7.3 / 1.5 / 2.5 / 4.3 / 1.8 / 0
Mean / 8.9 / 3.9 / 3.4 / 30.1 / 13.9 / 1.4 / 18 / 5 / 5.3 / 45.8 / 18.9 / 3.4 / 4.7 / 5.4 / 4.3 / 0
Std. Error / 2.3 / 1.3 / 0.7 / 8.2 / 4 / 0.4 / 5.6 / 1.1 / 1.5 / 15.4 / 6.2 / 1 / 1.3 / 1.4 / 1.7 / 0
Minimum / 0 / 0.5 / 0 / 0.5 / 2 / 0 / 0.5 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Maximum / 28.5 / 20 / 9 / 114.5 / 65 / 4 / 67.5 / 11.5 / 18.5 / 232.5 / 89.5 / 11.5 / 15.5 / 17.5 / 25 / 0
Morphine 1 mg/kg
Median / 12.5 / 5.3 / 3.8 / 20.5 / 13.5 / 1 / 23.8 / 5.5 / 7.8 / 29.5 / 15 / 3.3 / 6.5 / 9.8 / 2.8 / 0
Mean / 14.5 / 6.2 / 4.3 / 33.7 / 13.3 / 2.2 / 22 / 7.3 / 7.4 / 69.7 / 33.5 / 5.3 / 7.8 / 10.3 / 4.8 / 0
Std. Error / 3.2 / 1.5 / 0.6 / 8.7 / 2.7 / 0.8 / 5.2 / 1.8 / 1.6 / 19.7 / 10.9 / 1.6 / 1.8 / 2.4 / 1.6 / 0
Minimum / 0.5 / 0.5 / 0.5 / 2 / 0.5 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Maximum / 40.5 / 20.5 / 8.5 / 113.5 / 39 / 10 / 78.5 / 25.5 / 21 / 247.5 / 161.5 / 21.5 / 21.5 / 29 / 24 / 0
Morphine 3 mg/kg
Median / 4.5 / 1.8 / 1 / 9.3 / 3.8 / 1 / 4.5 / 2 / 1 / 7 / 4.5 / 1.5 / 2.3 / 3.5 / 1.3 / 0
Mean / 12.8 / 4.5 / 3.1 / 18.7 / 7.6 / 2.5 / 12.3 / 5.7 / 4.7 / 53.7 / 22.4 / 5 / 8.2 / 9.5 / 4.5 / 0
Std. Error / 4.7 / 1.7 / 1 / 6.3 / 2.2 / 0.8 / 4.7 / 2.9 / 1.6 / 23.7 / 10.9 / 2.2 / 3 / 3.8 / 1.6 / 0
Minimum / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Maximum / 70.5 / 25 / 13 / 97.5 / 26 / 9 / 69 / 47 / 19.5 / 328 / 153.5 / 28 / 39 / 52 / 18 / 0

Notes:

Values represent the number of calls emitted per 20-min session for each call subtype. In general, the data were positively skewed, so that mean values tended to be larger than the corresponding medians. n=16 rats, repeated measures design.

Call subtype abbreviations: CX complex, UR upward ramp, DR downward ramp, FL flat, SH short, SP split, SU step-up, SD step-down, MS multi-step, TR trill, FT flat-trill, TJ trill with jumps, IU inverted-U, CS composite, UC unclear, MI miscellaneous.

Morphine significantly increased the absolute rate of trill calls, at both 0.3 and 1 mg/kg (Z=2.22 and 2.36, p=0.0268 and 0.0183, respectively), whereas flat calls were not significantly affected.