Electronic Supplementary Material 1 Examples of Platanus × acerifolia accessions, derived by cutting from spontaneous and cultivated urban ortets, that were highly susceptible to Ceratocystis platani inoculation, some of which were inoculated in different years as controls
Accessions / Noramets / Inoc.a year / Inoc.a age / Øb
mm / Survival days / Canker evaluation
B2 / 1 / 2003 / 3 / 23 / 106 / I
MR3 / 1 / 4 / 23 / 157 / IV
MS7 / 1 / 4 / 22 / 177 / V
MS20b / 1 / 4 / 29 / 150 / II
N7 / 3 / 3 / 22–27 / 118–198 / I, III
E6 / 2 / 3 / 25, 27 / 119, 171 / I, II
N10 / 1 / 3 / 22 / 90 / IV
C6 / 1 / 3 / 27 / 462 / II
MS21 / 4 / 2004 / 4 / 29–31 / 92–162 / I
E9 / 1 / 3 / 26 / 90 / I
E4 / 1 / 3 / 29 / 101 / I
B3 / 4 / 3 / 24–28 / 97–118 / III
N9 / 2 / 4 / 24, 28 / 105, 141 / I
N10 / 1 / 4 / 23 / 108 / I
N7 / 2 / 4 / 26, 28 / 99, 101 / I, II
V4 / 2 / 2 / 20, 29 / 94, 98 / I
S2 / 1 / 4 / 22 / 92 / I
S4 / 1 / 2 / 21 / 69 / I
N6 / 2 / 4 / 28, 33 / 101, 196 / I
N8 / 1 / 5 / 25 / 98 / I
E10 / 7 / 2005 / 3 / 25–30 / 99–128 / I
P1 / 2 / 3 / 21, 26 / 141, 171 / II, IV
E8 / 1 / 5 / 31 / 313 / III
N7 / 2 / 5 / 26, 30 / 138–173 / II, III
P3 / 1 / 3 / 24 / 312 / III
E9 / 3 / 2006 / 5 / 27–31 / 101–203 / I, III, IV
E7 / 1 / 4 / 26 / 100 / I
E10 / 2 / 4 / 31, 32 / 118 / I
N6 / 1 / 5 / 25 / 266 / I
N4 / 1 / 3 / 21 / 203 / II
MS14 / 2 / 2007 / 4 / 29, 33 / 93, 104 / I
MS8 / 1 / 4 / 32 / 109 / I
MS7 / 1 / 4 / 30 / 85 / I
a Inoc.=Inoculation. b Diameter of the trunk at the inoculation point at the time of inoculation.
Electronic Supplementary Material 2 ITS nucleotide identities (%) among ITS regions of different species within Platanaceae, as reported by Feng et al. (2005), and the ITS regions from the stably surviving accessions (SSAs) of P.× acerifolia that did not strictly group, in the phylogenetic tree (Fig. 3), with known Platanus species
ITS sequencesa / P. kerry / P. orientalis / P. racemosa / P. occidentalis / P. mexicana / C2.51 / B5.53 / MS.12.27 / MS.12.29 / B5.57 / E3.1 / E3.4 / S8.152 / S8.153 / S8.157 / S8.160 / S8.161 / S8.169V2/58.5 / 89 / 92 / 92 / 92 / 92 / 92 / 92 / 89 / 89 / 90 / 89 / 94 / 89 / 87 / 88 / 90 / 93 / 92
S8.169 / 92 / 95 / 95 / 95 / 95 / 95 / 95 / 91 / 92 / 92 / 97 / 89 / 93 / 88 / 89 / 92 / 96
S8.161 / 94 / 97 / 97 / 97 / 97 / 97 / 97 / 92 / 94 / 95 / 93 / 90 / 94 / 90 / 91 / 95
S8.160 / 94 / 95 / 96 / 96 / 96 / 95 / 96 / 89 / 93 / 98 / 93 / 89 / 97 / 90 / 92
S8.157 / 89 / 92 / 92 / 91 / 92 / 92 / 91 / 88 / 90 / 91 / 89 / 88 / 90 / 96
S8.153 / 88 / 91 / 91 / 90 / 90 / 91 / 90 / 88 / 88 / 89 / 87 / 86 / 89
S8.152 / 93 / 95 / 95 / 96 / 95 / 95 / 95 / 89 / 93 / 98 / 96 / 89
E3.4 / 88 / 91 / 91 / 89 / 90 / 91 / 89 / 87 / 90 / 90 / 89
E3.1 / 91 / 94 / 94 / 92 / 93 / 94 / 92 / 89 / 92 / 95
B5.57 / 94 / 95 / 96 / 97 / 96 / 95 / 97 / 90 / 94
MS.12.29 / 92 / 96 / 95 / 94 / 95 / 96 / 94 / 89
MS.12.27 / 89 / 93 / 92 / 91 / 91 / 93 / 91
B5.53 / 95 / 97 / 97 / 100 / 98 / 97
C2.51 / 94 / 100 / 98 / 97 / 97
P. mexicana / 95 / 97 / 97 / 98
P. occidentalis / 95 / 97 / 97
P. racemosa / 95 / 98
P. orientalis / 94
a The ITS sequences included for comparison are shaded grey: P. kerry (AY706018), P. occidentalis, (AY706030) P. mexicana (AY706020), P. orientalis (AY706034), P. racemosa (AY706038) are from Feng et al. (2005); C2.51 and B5.53 were obtained in this work from the SSAs (C2 and B5 respectively) and showed complete identity with P. orientalis and P. occidentalis respectively.