LOCUS pRS316-Bir 6800 bp DNA circular 8-AUG-2006
DEFINITION ATCC 77145: Yeast centromere vector pRS316 with URA3 marker, complete sequence.
KEYWORDS ATCC.
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT ORIGDB|GenBank
COMMENT VNTDATE|38836800|
COMMENT VNTDBDATE|418208084|
COMMENT VNTNAME|pRS316-BirA-NLS|
COMMENT VNTAUTHORNAME|Demo User|
COMMENT VNTOAUTHORNAME|NCBI Entrez|
COMMENT VNTOAUTHORTEL|(301)496-2475|
COMMENT VNTOAUTHORFAX|(301)480-9241|
COMMENT VNTOAUTHOREML||
COMMENT VNTOAUTHORWWW|http://www3.ncbi.nlm.nih.gov/Entrez/|
COMMENT VNTOAUTHORAD1|National Center for Biotechnology Information|
COMMENT VNTOAUTHORAD2|National Library of Medicine|
COMMENT VNTOAUTHORAD3|Building 38A, Room 8N805|
COMMENT VNTOAUTHORAD4|Bethesda, MD 20894, U.S.A.|
COMMENT VNTREPLTYPE|Phagemid
COMMENT VNTEXTCHREPL|Bacteria
COMMENT VNTEXTCHREPL|Yeast
FEATURES Location/Qualifiers
rep_origin 4468..4468
/vntifkey="33"
/label=PMB1
/note="origin of replication"
CDS 411..1214
/vntifkey="4"
/label=URA3
/note="orotidine-5'-phosphate"
misc_feature 6228..6344
/vntifkey="21"
/label=CEN6
/note="centromere VI"
rep_origin 1567..1666
/vntifkey="33"
/label=F1_ORI
/note="origin of replication"
promoter complement(4025..4044)
/vntifkey="30"
/label=T3_P
/note="T3 promoter"
promoter 1966..1985
/vntifkey="30"
/label=T7_P
/note="T7 promoter"
CDS complement(5226..6086)
/vntifkey="4"
/label=AP(R)
/note="bla beta-lactamase, ampR ampicillin resistance"
misc_feature 6345..6719
/vntifkey="21"
/label=ARSH4
/note="autonomous replicating sequence histone 4"
promoter 2028..2407
/vntifkey="29"
/label=PGK
terminator 3613..3986
/vntifkey="43"
/label=CYC1
sig_peptide 2461..2517
/vntifkey="94"
/label=NLS
misc_feature 2518..2625
/vntifkey="21"
/label=HA
CDS 2626..3591
/vntifkey="4"
/label=BirA
BASE COUNT 1853 a 1485 c 1650 g 1812 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca
1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
1981 tagggcgaat tggagctcca ccgcggtggc ggccgctatc gctacgtgac tgggtcatgg
2041 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
2101 catccgctta cagacaagct gtggccgtct ccgggagctg catgtgtcag aggttttcac
2161 cgtcatcacc gaaacgcgcg aggcagagat cccgactctt ttcttctaac caagggggtg
2221 gtttagttta gtagaacctc gtgaaactta catttacata tatataaact tgcataaatt
2281 ggtcaatgca agaaatacat atttggtctt ttctaattcg tagtttttca agttcttaga
2341 tgctttcttt ttctcttttt tacagatcat caaggaagta attatctact ttttacaaca
2401 aatataaaac aagatctcga ctctagagga tcccccgggc tgcaggaatt cgccgccacc
2461 atgggtgctc ctccaaaaaa gaagagaaag gtagctggta tcaataaaga catcgccacc
2521 tatgacgtcc cagattacgc aagtttgccc gggtatgatg ttcctgatta tgctagcctc
2581 ccgggttacg atgtgcccga ctatgcctca cttccaggca gatctatgaa ggataacacc
2641 gtgccactga aattgattgc cctgttagcg aacggtgaat ttcactctgg cgagcagttg
2701 ggtgaaacgc tgggaatgag ccgggcggct attaataaac acattcagac actgcgtgac
2761 tggggcgttg atgtctttac cgttccgggt aaaggataca gcctgcctga gcctatccag
2821 ttacttaatg ctaaacagat attgggtcag ctggatggcg gtagtgtagc cgtgctgcca
2881 gtgattgact ccacgaatca gtaccttctt gatcgtatcg gagagcttaa atcgggcgat
2941 gcttgcattg cagaatacca gcaggctggc cgtggtcgcc ggggtcggaa atggttttcg
3001 ccttttggcg caaacttata tttgtcgatg ttctggcgtc tggaacaagg cccggcggcg
3061 gcgattggtt taagtctggt tatcggtatc gtgatggcgg aagtattacg caagctgggt
3121 gcagataaag ttcgtgttaa atggcctaat gacctctatc tgcaggatcg caagctggca
3181 ggcattctgg tggagctgac tggcaaaact ggcgatgcgg cgcaaatagt cattggagcc
3241 gggatcaaca tggcaatgcg ccgtgttgaa gagagtgtcg ttaatcaggg gtggatcacg
3301 ctgcaggaag cggggatcaa tctcgatcgt aatacgttgg cggccatgct aatacgtgaa
3361 ttacgtgctg cgttggaact cttcgaacaa gaaggattgg caccttatct gtcgcgctgg
3421 gaaaagctgg ataattttat taatcgccca gtgaaactta tcattggtga taaagaaata
3481 tttggcattt cacgcggaat agacaaacag ggggctttat tacttgagca ggatggaata
3541 ataaaaccct ggatgggcgg tgaaatatcc ctgcgtagtg cagaaaaata atgaccatgg
3601 catcactagt cgaggggggg cccggtacca agatggcctt tggtgggttg aagaaggaaa
3661 aagacagaaa cgacttaatt acctacttga aaaaagcctg tgagtaaaca ggcccctttt
3721 cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat
3781 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
3841 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt
3901 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaac gttttgggac
3961 gctcgaaggc tttaatttgc aagcttgtcg acctcgaggg ggggcccggt acccagcttt
4021 tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata gctgtttcct
4081 gtgtgaaatt gttatccgct cacaattcca cacaacatag gagccggaag cataaagtgt
4141 aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc
4201 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
4261 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
4321 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
4381 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
4441 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcg gcccccctga cgagcatcac
4501 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
4561 ttcccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
4621 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
4681 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
4741 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
4801 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
4861 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
4921 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
4981 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
5041 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
5101 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
5161 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
5221 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
5281 tccatagttg cctgactgcc cgtcgtgtag ataactacga tacgggaggg cttaccatct
5341 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
5401 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
5461 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
5521 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
5581 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgaaaa
5641 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
5701 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
5761 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
5821 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
5881 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
5941 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
6001 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
6061 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
6121 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
6181 ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt ccttttcatc acgtgctata
6241 aaaataatta taatttaaat tttttaatat aaatatataa attaaaaata gaaagtaaaa
6301 aaagaaatta aagaaaaaat agtttttgtt ttccgaagat gtaaaagact ctagggggat
6361 cgccaacaaa tactaccttt tatcttgctc ttcctgctct caggtattaa tgccgaattg
6421 tttcatcttg tctgtgtaga agaccacaca cgaaaatcct gtgattttac attttactta
6481 tcgttaatcg aatgtatatc tatttaatct gcttttcttg tctaataaat atatatgtaa
6541 agtacgcttt ttgttgaaat tttttaaacc tttgtttatt tttttttctt cattccgtaa
6601 ctcttctacc ttctttattt actttctaaa atccaaatac aaaacataaa aataaataaa
6661 cacagagtaa attcccaaat tattccatca ttaaaagata cgaggcgcgt gtaagttaca
6721 ggcaagcgat ccgtcctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
6781 tatcacgagg ccctttcgtc
//