Table S1. List of PCR primers used for generation of constructs for expression different TMV MP mutants.

Construct name / Primer namea / Primer sequence 5’->3’b
pSAT1-TMV MP1-50-CFP / MP50REcoRI / gcgcGAATTCcaatgactcattctcatgaaccattattttatcaact
CFPFEcoRI / gtttGAATTCtgcagtcgacttatggtga
pSAT1-TMV MP1-100-CFP / MP100REcoRI / gcgcGAATTCggctctttccatccttttgtccac
CFPFEcoRI / gtttGAATTCtgcagtcgacttatggtga
pSAT1-TMV MP20-50-CFP / MP20FBamHI / gcgGGATCCATGatggagaagatcttaccgtcgatgttt
MPRBamHI / gcgGGATCCagtcgagatctgagtccggacc
pSAT1-TMV MP30-50-CFP / MP30FBglII / ctcAGATCTcgactATGcctgtaaagagtgttatgtgttccaaagttg
MPRBamHI / gcgggatccagtcgAGATCTgagtccggacc
pSAT1-TMV MP51-268-CFP / MP51FBglII / ctcAGATCTcgactATG tcagaggtgaaccttcttaaaggagttaagc
MP268REcoRI / gtcgactgcaGAATTCaaacgaatcc
pSAT1-TMV MP218-268-CFP / MP218FBglII / ctcAGATCTcgactATG agtgatgtccgcaaagggaaaaatag
MP268REcoRI / gtcgactgcaGAATTCaaacgaatcc
pSAT1-TMV MP1-10-CFP / MP10REcoRI / gcgcGAATTCattcacttttcctttaacaactagagccatg
CFPFEcoRI / gtttGAATTCtgcagtcgacttatggtga
pSAT1-TMV MP1-20-CFP / MP20REcoRI / gcgcGAATTCcatttttgacaggtcgataaactcattgatattcac
CFPFEcoRI / gtttGAATTCtgcagtcgacttatggtga
pSAT1-TMV MP1-30-CFP / MP30REcoRI / gcgcGAATTCaggggtaaacatcgacggtaagatc
CFPFEcoRI / gtttGAATTCtgcagtcgacttatggtga
pSAT1-TMV MP1-50(V4A)-CFP / 1-50ASMP4F / gatctcgacatggctctagctgttaaaggaaaagtg
1-50ASMP4R / cacttttcctttaacagctagagccatgtcgagatc
pSAT1-TMV MP1-50(G7A)-CFP / 1-50ASMP7F / ctagttgttaaagcaaaagtgaatatcaatgagtttatcg
1-50ASMP7R / cgataaactcattgatattcacttttgctttaacaactag
pSAT1-TMV MP1-50(I11A)-CFP / 1-50ASMP11F / ggaaaagtgaatgccaatgagtttatcgacctg
1-50ASMP11R / caggtcgataaactcattggcattcacttttcc
pSAT1-TMV MP1-50(F14A)-CFP / 1-50ASMP14F / gaatatcaatgaggctatcgacctgtca
1-50ASMP14R / tgacaggtcgatagcctcattgatattc
pSAT1-TMV MP1-50(D16A)-CFP / 1-50ASMP16F / caatgagtttatcgccctgtcaaaaatggag
1-50ASMP16R / ctccatttttgacagggcgataaactcattg
pSAT1-TMV MP1-50(S18A)-CFP / 1-50ASMP18F / gtttatcgacctggcaaaaatggagaagatc
1-50ASMP18R / gatcttctccatttttgccaggtcgataaac
pSAT1-TMV MP1-50(E21A)-CFP / 1-50ASMP21F / cgacctgtcaaaaatggcgaagatcttaccgtcg
1-50ASMP21R / cgacggtaagatcttcgccatttttgacaggtcg
pSAT1-TMV MP1-50(L24A)-CFP / 1-50ASMP F / ggagaagatcgcaccgtcgatgtt
1-50ASMP R / aacatcgacggtgcgatcttctcc
pSAT1-TMV MP1-50(P25A)-CFP / 1-50ASMP25F / ggagaagatcttagcgtcgatgtttacccctg
1-50ASMP25R / caggggtaaacatcgacgctaagatcttctcc
pSAT1-TMV MP1-50(T29A)-CFP / 1-50ASMP29F / gatcttaccgtcgatgtttgcccctgtaaagagtgttatg
1-50ASMP29R / cataacactctttacaggggcaaacatcgacggtaagatc
pSAT1-TMV MP1-50(K32A)-CFP / 1-50ASMP32F / cgatgtttacccctgtagcgagtgttatgtgttcc
1-50ASMP32R / ggaacacataacactcgctacaggggtaaacatcg
pSAT1-TMV MP1-50(S37A)-CFP / 1-50ASMP37F / gtaaagagtgttatgtgtgccaaagttgataaaataatgg
1-50ASMP37R / ccattattttatcaactttggcacacataacactctttac
pSAT1-TMV MP1-50(K38A)-CFP / 1-50ASMP38F / gtgttatgtgttccgcagttgataaaataatgg
1-50ASMP38R / ccattattttatcaactgcggaacacataacac
pSAT1-TMV MP1-50(D40A)-CFP / 1-50ASMP40F / tgtgttccaaagttgctaaaataatggttcatg
1-50ASMP40R / catgaaccattattttagcaactttggaacaca
pSAT1-TMV MP1-50(N47A)-CFP / 1-50ASMP47F / taatggttcatgaggctgagtcattgtcagagg
1-50ASMP47R / cctctgacaatgactcagcctcatgaaccatta
pSAT1-TMV MP1-50(L50A)-CFP / 1-50ASMP50F / gagaatgagtcagcggaattctgcagtc
1-50ASMP50R / gactgcagaattccgctgactcattctc
pSAT1-TMV MPTMV(V4A)-CFP / 1-50ASMP4F / gatctcgacatggctctagctgttaaaggaaaagtg
1-50ASMP4R / cacttttcctttaacagctagagccatgtcgagatc
pSAT1-TMV MPTMV(F14A)-CFP / 1-50ASMP14F / gaatatcaatgaggctatcgacctgtca
1-50ASMP14R / tgacaggtcgatagcctcattgatattc

(a) Letters R and F in primer names indicate reverse and forward primers, respectively.

(b) Capital letters in primer sequences indicate EcoRI (GAATTC),BamHI (GGATCC), orBglIsites (AGATCT) or the translation initiation site (ATG).