CHE 3211 and 3281
CHEMISTRY 3211 and 3281
INSTRUMENTAL ANALYSIS
SYLLABUS --
Spring 2002
2001-2002 Catalog:3211 - CREDIT (3-5-5) Introduction to the theory and practice of modern chemical analysis
3281 - CREDIT (3-3-4) Provides a background to modern analytical chemistry and to chemical instrumentation methods of analysis with applications to engineering and other areas.
Text:Skoog, Holler and Nieman: Principles of Instrumental Analysis 5th edition, Saunders 1998
Instructor:Dr. Boris Mizaikoff
Web Site: then select 3211 or 3281
Detailed Description:The Course is designed to cover the theoretical principles and practical operation of a variety of instrumental analytical techniques. The topics include: atomic and molecular spectroscopies, mass spectrometry, electroanalytical techniques, chromatographic separations and thermal analysis. The lecture part of the course is common for 3211 and 3281. The differentiation is in laboratory section and in grading.
Educational Objectives:This course provides students with the theoretical background necessary for understanding the principles behind selected modern analytical instrumentation. Students gain practical experience in the laboratory equipped with modern instrumentation (similar to that employed by practicing scientists and engineers in industry or graduate school) used to solve current analytical problems.
Midterm Exam Dates:M1: 2/1; M2: 3/1; M3: 4/5; Final (in the week of 4/29-5/3)
Review Classes:4/3 and 4/26
Grading:32113281
Lab35 points30 points
Homework15 points15 points
I Midterm10 points10 points
II Midterm10 points10 points
III Midterm10 points10 points
Final Exam20 points25 points
Total100 points100 points
Grading Key:A: 100-85; B: 84-70; C: 69-55; D: 54-40; F: 39-0
Lecture Contents:(Book Chapter)Date
Overview (1 hr).(Chapter 1)1/4
Course objectives and mechanics
Analytical strategy
Classification and selection of analytical methods
Figures of merit (1 hr)(Chapter 1)1/7
Types of instrumental methods
Precision, accuracy, LOD, etc.
Properties of Electromagnetic Radiation (2 hrs)(Chapter 6)1/9-1/11
Types of Electromagnetic Radiation
Nature of Light
Basic Optical Parameters
Quantum Mechanical Basis of Radiation
Term Diagrams
Instruments for Optical Spectroscopy (3 hrs)(Chapter 7)1/14-1/18
Fundamental Components of Optical Instruments
Gratings, Slits, Dispersion, Resolving Power
Radiation Sources
Continuum, Line sources
Wavelength detectors
Photomultipliers, Photodiode Arrays, Charge Coupled Detectors
Signal Processors and Readout
Fiber Optics
FT instrumentation
School Holiday1/21
Atomic Spectrometry - Flame and Electrothermal (3 hrs)(Chapter 8,9)1/23-1/28
Atom Formation in Flames
Nebulizers, Flame Types
Chemical Equilibria in Flame (Element Ionization and Oxidation Equilibria)
Atomic Radiation Sources
Hollow Cathode Lamps and RF Electrodeless Discharge Lamps
Spectral, Matrix, and Ionization Interferences
Atom Formation in Electrothermal Vaporizer
Interferences: Scatter, Matrix Interactions
Emission Spectroscopy Based on RF Plasma Excitation (1 hr)(Chapter 10)1/30
RF Inductively Coupled Plasma
Simultaneous Multielement Analysis
Midterm 12/1
UV/Visible And Near Infrared Spectroscopy (2 hrs)(Chapter 13)2/4-2/6
Beer-Lambert Law, Basics of Absorption Spectroscopy
Quantitative Aspects of Absorption Spectroscopy
Chemical and Physical Errors in Beer-Lambert Law
Instrumentation for UV, Visible and Infrared Spectroscopy
Instruments for Separation in Space, Separation in Time
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CHE 3211 and 3281
Applications of Molecular Spectroscopy (2 hrs)(Chapter 14)2/8-2/11
Molecular Absorption Spectroscopy - Quantitative Aspects
Application to Non-absorbing Species, Standard Additions Method, Analysis of Mixtures
Dual-wavelength Measurements, Photoacoustic Spectroscopy
Luminescence Spectroscopy (2 hrs)(Chapter 15)2/13-2/15
Theory of fluorescence and phosphorescence
Instrumentation
Chemi- and bioluminescence
Applications
Introduction to Infrared Spectrometry (2 hrs)(Chapter 16)2/18-2/20
Theory
Instrumentation
Applications
Introduction to X-Ray Spectrometry (1 hr)(Chapter 12)2/22
Theory
Instrumentation
Applications
Introduction to Chromatographic Separations (2 hrs)(Chapter 26)2/25-2/27
General Concepts
Species Migration Rates
Band Broadening and Column Efficiency
Column Performance Optimization
Midterm 23/1
Spring Break3/4-3/8
Gas Chromatography (2 hrs)(Chapter 27)3/11-3/13
Principles of Gas-Liquid Chromatography
Column Efficiency in Gas-Liquid Chromatography
Instrumentation for Gas-Liquid Chromatography
Columns and Stationary Phases
Applications
High Performance Liquid Chromatography (4 hrs)(Chapter 28)3/15-3/22
Relationship of HPLC to GC
Column Efficiency in Liquid Chromatography
Instruments for High Performance Liquid Chromatography
Types of Liquid High Performance Chromatography
Partition, Adsorption, Ion, and Size-Exclusion Chromatography
Other Separation Methods (1 hr)(Chapter 29,30)3/25
Supercritical Fluid Chromatography
Capillary Electrophoresis
Mass Spectrometry (2 hrs)(Chapter 20)3/27-3/29
Mass Spectrometer Design and Features
Gas Chromatography/Mass Spectrometry, Liquid Chromatography/Mass Spectrometry
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CHE 3211 and 3281
Thermal Methods (1 hr)(Chapter 31)4/1
Thermogravimetric Methods
Differential Thermal Analysis
Differential Scanning Calorimetry
Review Class4/3
Midterm 34/5
Electroanalytical Chemistry (2 hrs)(Chapter 22)4/8-4/10
Types of electroanalytical techniques
Currents in electrochemical cells
Potentiometry (3 hrs)(Chapter 23)4/12-4/17
Reference electrodes
Indicator electrodes
Instrumentation
Voltammetry (3 hrs)(Chapter 24)4/19-4/24
Excitation signals
Instrumentation
Cyclic voltammetry
Polarography
Stripping methods
Microelectrodes
Review Class4/26
Finals4/29-5/3
LABORATORY:
3211: Coordinator Dr. Mira Josowicz (Dr. Christine Kranz)
TAs:Nick Menegazzo, Jamie Maddox, Isao Sasaki, Arteum Bochevarov,
George Lountos, Christine Nolan
Lab Manual:Chem 3211 Instrumental Analysis (handouts)
3281: Coordinator Dr. Christine Kranz
TAsAlexandra Molinelli, Christy Charlton, Tiffany Cao, Neil Pennington,
Manfred Karlowatz
Lab Manual:Chem 3281 Instrumental Analysis for Engineers
LIST OF HOMEWORKS:
January:7., 14., 21., 28.
February:4., 11., 18., 25.
March:11., 18., 25.
April:1., 8., 15., 22.
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