Table S1. Effect of jasmonic acid concentration and herbivory on chemical leaf traits in lima bean. Differences in chemical leaf traits among plants treated with different concentrations of aqueous jasmonic acid solution (0, 0.001, 0.01, 0.1 and 1.0 mmol L-1) or herbivore damage, but incubated for identical time periods prior the feeding trials (0, 12, 24, 48, 72, 96, and 192 h respectively; see Fig. 1) were analyzed with one-way ANOVAs; N=7 plants per treatment and period of incubation (N=252 plants in total).

Plant trait / Incubation period (h) / SS / df / MS / F / P
β-glucosidase activity / 0 / 0.026 / 5 / 0.005 / 0.027 / 1.000
6.891 / 36 / 0.191
12 / 48.038 / 9.608 / 0.661 / 0.655
523.246 / 14.535
24 / 892.970 / 178.594 / 21.458 / <0.001
299.627 / 8.323
48 / 1172.102 / 234.420 / 15.869 / <0.001
531.809 / 14.772
72 / 1096.313 / 219.263
484.422 / 13.456 / 16.295 / <0.001
96 / 1073.421 / 214.684 / 29.275 / <0.001
264.003 / 7.333
192 / 979.098 / 195.820 / 11.269 / <0.001
625.546 / 17.376
HCNp / 0 / 6.442 / 1.288 / 0.192 / 0.964
241.283 / 6.702
12 / 41.061 / 8.212 / 1.802 / 0.137
164.040 / 4.557
24 / 6.559 / 1.312 / 0.124 / 0.986
379.360 / 10.538
48 / 31.242 / 6.248 / 0.724 / 0.610
310.903 / 8.636
72 / 21.682 / 4.336 / 0.628 / 0.679
248.503 / 6.903
96 / 2.442 / 0.488 / 0.060 / 0.997
293.549 / 8.154
192 / 9.643 / 1.929 / 0.279 / 0.922
248.843 / 6.912
HCNc / 0 / 1.212 / 5 / 0.242 / 0.131 / 0.984
66.834 / 36 / 1.857
12 / 204.219 / 40.844 / 1.103 / 0.376
1333.438 / 37.040
24 / 1776.876 / 355.375 / 32.863 / <0.001
389.293 / 10.814
48 / 2091.104 / 418.221 / 29.732 / <0.001
506.381 / 14.066
72 / 1959.730 / 391.946 / 34.255 / <0.001
411.911 / 11.442
96 / 1958.098 / 391.620 / 43.929 / <0.001
320.936 / 8.915
192 / 1877.682 / 375.536 / 19.418 / <0.001
696.225 / 19.340
Soluble protein / 0 / 0.044 / 0.009 / 0.014 / 1.000
22.237 / 0.618
12 / 9.382 / 0.542 / 3.461 / 0.012
19.517
24 / 13.693 / 2.739 / 5.952 / <0.001
16.566 / 0.460
48 / 20.299 / 4.060 / 13.280 / <0.001
11.006 / 0.306
72 / 16.179 / 3.236 / 7.075 / <0.001
16.466 / 0.457
96 / 17.370 / 3.474 / 12.825 / <0.001
9.751 / 0.271
192 / 19.884 / 3.977 / 7.723 / <0.001
18.537 / 0.515