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Biological Material Testing for Murine Pathogens

TABLE OF CONTENTS

  1. Introduction
  2. Procedures
  3. References
  4. Appendix

1.  INTRODUCTION

Biological material refers to cell lines, transplantable tumors, serum, tissues, body fluids, antibody preparations or hybridoma lines. When biological material is introduced into an animal, it is a potential source of contamination by adventitious pathogens. Pathogens present in these materials have the potential to infect animal that are inoculated with them.

Biological materials must be tested for specific pathogens from a CM approved diagnostic laboratory (e.g. IDEXX RADIL, Charles River), prior to use in animals. Please refer to Appendix A for the list of murine pathogens to be tested.

Allow 1-2 weeks from the time of sample submission until results are received.

The cost of biological material testing is currently borne by CM.

Non-animal users are not allowed to use the irradiator(s) located within a NUS/CM vivarium for their biological materials. Please contact Life Sciences Institute (LSI) for use of their irradiator.

2.  PROCEDURES

a.  Biologics that REQUIRE testing prior to in vivo murine use:

i.  Murine-derived cell lines, transplantable tumors, serum, tissues, body fluids, antibody preparations and hybridomas from originating from rodent colonies outside of CM facilities.

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ii.  Non-murine (including human) derived cell lines, transplantable tumors, serum, tissues, body fluids, antibody preparations and hybridomas that have been passaged through rodents or have been exposed to rodents outside CM animal facilities.

b.  Possible exemptions from testing: Exemptions must be approved by CM.

i.  If the murine biological material is derived from donor animals located in the same facility and room as the recipients (or approved room within the same facility with the same health status).

ii.  Non-murine derived biological that have NOT been passaged through rodent or exposed to murine products.

iii.  Commercially obtained biologicals for which the vendor can supply negative screening results, satisfactory to CM.

c.  Submitting samples:

i.  Contact CM at 65166410 or to arrange testing of biological material.

ii.  Submit samples to CM Vet Diagnostic Laboratory.

a.  Complete submission form. Request for Pathogen Screening for Biological Materials. (Appendix B)

b.  Submit two 1ml cryovial of each cell line sample containing a minimum of minimum of 2 x 106 cells. Cells can be in pellet form, in freeze media, growth media, or PBS.

c.  For liquid samples, submit two 1ml cryovials with at least 0.5ml.

d.  Collect all samples aseptically

e.  Label all samples with PI name and sample ID (cell line designation).

f.  Contact CM Vet Diagnostic Lab at 6516 6410 for further assistance.

3.  REFERENCES

·  RADIL links: http://www.radil.missouri.edu/Biological_Materials/Cell_Line_Authentication_Cell_Check_/index.html

·  Charles River – Research Animal Diagnostics http://www.criver.com/sitecollectiondocuments/rm_ld_d_sample_submission_info.pdf

·  NUS OSHE: http://www.nus.edu.sg/osh/index.html

·  Cornell- http://www.research.cornell.edu/care/documents/ACUPs/ACUP619.pdf

Revision # Author Effective Date SOP #:

.02 Anna Acuna 17 July 2012 605.02

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4.  APPENDIX

Appendix A

Mouse Pathogens / Rat Pathogens
Mycoplasma spp. / Kilham’s rat virus
Sendai virus / Toolan’s H1 virus
Mouse hepatitis virus / Rat parvovirus
Pneumonia virus of mice / Rat cytomegalovirus
Minute virus of mice / Rat coronavirus
Mouse parvovirus (MPV-1,2,3) / Rat minute virus
Theiler’s murine encephalomyelitis virus / Sialodacryoadenitis virus
Murine norovirus / Seoul virus
Reovirus-3 / Mycoplasma spp
Mouse rotavirus / Pneumonia virus of mice
Ectromelia virus / Lymphocytic choriomeningitis virus
Lymphocytic choriomeningitis virus / Sendai virus
Polyoma virus / Mouse adenovirus
Lactate dehydrogenase-elevating virus / Reovirus3
Mouse adenovirus (MAD-1,2) / Rat Theilovirus
Mouse Cytomegalovirus
K virus
Mouse Thymic virus
Hantaan virus

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Appendix B

Date: ______

Please complete the information below:

Protocol #: ______OSHE Risk Assessment #______

Primary Investigator: ______Email/Phone ______

Laboratory Contact: ______Email/Phone______

In which animal facility and room will this biological material be used in?______

The biological material is:

1) Murine-derived: Yes No

If yes, is it derived from donor animals located in the same facility and room as the recipients (or approved room within the same facility with the same health status)?

Yes No

Facility and Room No.:

2) Non- Murine (including human) derived cell lines

Yes No

If yes, has it been passaged through rodents or exposed to murine products?

Yes No

If yes, was it passaged through rodents or exposed to murine products in the same room as the recipients or approved room within the same facility with the same health status ?

Facility and Room No. :______

Samples submitted

Name / Description / Origin / Media
Example: Hep93C / Human hepatocellular carcinoma line / PBS
1.
2.

*Attach additional sheets if necessary

Additional comments: