Table S1 Onion probes tentatively annotated as ethylene-related up and down-regulated after treatment with ethylene before curing (EB), 1-MCP before curing (MB) or ethylene and 1-MCP before curing (EMB) for 24 h at 20°C or after long term storage in continuous ethylene (CE).

Probe / Tentative annotation / Fold changea / Regulation / Onion Sequence ID / % Identity
EB / EMB / MB / CE
CUST_230_PI403527117 / 1-aminocyclopropane-1-carboxylate oxidase protein / 3.72 / 2.60 / 5.33 / - / down / TC4068 / 61.29
CUST_4049_PI403527117 / ethylene receptor, putative / 2.77 / 1.96 / 2.65 / - / down / TC7887 / 71.97
CUST_11734_PI403527117 / ethylene-insensitive 3 / 2.71 / 3.57 / 3.58 / - / down / CF443937 / 82.12
CUST_1437_PI403527117 / 1-aminocyclopropane-1-carboxylate oxidase protein / 4.87 / 3.17 / 2.02 / - / up / TC5275 / 85.29
CUST_6695_PI403527117 / 1-aminocyclopropane-1-carboxylate deaminase / 3.29 / 4.84 / 3.28 / - / up / CF435987 / 79.07
CUST_10068_PI403527117 / 1-aminocyclopropane-1-carboxylate oxidase homolog 4 / 6.98 / 24.76 / 5.28 / - / up / CF438875 / 43.21
CUST_12127_PI403527117 / ethylene-insensitive protein 2 / 7.79 / 7.15 / 6.16 / - / up / BI095685 / 84.75
CUST_3987_PI403527117 / ethylene-insensitive 3 / - / - / - / 2.40 / up / TC7825 / 83.33
CUST_7606_PI403527117 / 1-aminocyclopropane-1-carboxylate oxidase homolog 2 / - / - / - / 3.58 / up / CF449970 / 50.78

a Fold change compared with expression of control, calculated as 2x, where x = absolute value of (normalised treatment / normalised control)

Table S2 Onion probes tentatively annotated as defence-related up and down-regulated after treatment with continuous ethylene.

Probe / Tentative annotation / Fold change a / Regulation / Onion Sequence ID / % Identity
CUST_10123_PI403527117 / periplasmic beta-glucosidase precursor / 3.10 / down / CF442466 / 80.77
CUST_1065_PI404013528 / conserved oligomeric Golgi complex component 3 / 3.27 / down / CF448523 / 75.44
CUST_11533_PI403527117 / HEAT repeat family protein / 3.00 / down / CF446800 / 70.34
CUST_16_PI403527117 / glucan endo-1,3-beta-glucosidase precursor / 2.59 / down / TC3854 / 58.67
CUST_2709_PI403527117 / MSH-like DNA mismatch repair protein / 2.70 / down / TC6547 / 64.94
CUST_3038_PI403527117 / pectinesterase / 2.78 / down / TC6876 / 61.29
CUST_3787_PI403527117 / WAX2 / 2.03 / down / TC7625 / 58.06
CUST_586_PI403527117 / heat shock protein DnaJ / 2.66 / down / TC4424 / 57.58
CUST_6208_PI403527117 / DNAJ heat shock N-terminal domain-containing protein / 4.97 / down / CF439108 / 61.47
CUST_7194_PI403527117 / DNA mismatch repair protein MSH6-2 / 2.77 / down / CF437137 / 73.8
CUST_7233_PI403527117 / periplasmic beta-glucosidase precursor / 2.20 / down / CF437739 / 72.88
CUST_8026_PI403527117 / frigid / 2.44 / down / CF449088 / 56.64
CUST_8862_PI403527117 / glucan endo-1,3-beta-glucosidase precursor / 2.53 / down / CF437631 / 60.95
CUST_887_PI403527117 / heat shock protein DnaJ / 5.65 / down / TC4725 / 35.83
CUST_9528_PI403527117 / peroxidase precursor / 13.09 / down / CF446294 / 66.83
CUST_1781_PI403527117 / lipoxygenase protein / 2.23 / up / TC5619 / 61.68
CUST_1883_PI403527117 / plant viral response family protein / 5.90 / up / TC5721 / 55.75
CUST_2240_PI403527117 / LTPL8 - Protease inhibitor/seed storage/LTP family protein / 2.55 / up / TC6078 / 57.29
CUST_2562_PI403527117 / LTPL8 - Protease inhibitor/seed storage/LTP family protein / 2.42 / up / TC6400 / 54.84
CUST_3059_PI403527117 / thioredoxin / 2.63 / up / TC6897 / 45.24
CUST_308_PI404013528 / HVA22 / 6.25 / up / CF435556 / 74.19
CUST_340_PI403527117 / glucan endo-1,3-beta-glucosidase precursor / 2.85 / up / TC4178 / 64.44
CUST_4174_PI403527117 / heat shock protein DnaJ / 2.57 / up / CF438741 / 52.86
CUST_4665_PI403527117 / Fibronectin type III domain containing protein / 2.06 / up / CF436103 / 67.5
CUST_4799_PI403527117 / peroxidase precursor / 2.56 / up / CF446769 / 68.78
CUST_520_PI403527117 / peroxidase precursor / 4.12 / up / TC4358 / 65.78
CUST_7414_PI403527117 / trehalose synthase / 2.35 / up / CF451580 / 71.7
CUST_7435_PI403527117 / universal stress protein domain containing protein / 3.03 / up / CF451482 / 55.41
CUST_8803_PI403527117 / pathogenesis-related Bet v I family protein / 4.68 / up / CF452005

a Fold change compared with expression of control, calculated as 2x, where x = absolute value of (normalised treatment / normalised control)