Cirriculum Vitae

CIRRICULUM VITAE

John (Jay) L. Norelli

CURRICULUM VITAE

John (Jay) L. Norelli

Position: Research Plant Pathologist

Page 4

CIRRICULUM VITAE

John (Jay) L. Norelli

USDA-ARS

Appalachian Fruit Research Station

2217 Wiltshire Road

Kearneysville, WV 25430

Tel.: 304-725-3451 ext. 264

Fax: 304-725-0351

Email:

Page 4

CIRRICULUM VITAE

John (Jay) L. Norelli

Page 4

CIRRICULUM VITAE

John (Jay) L. Norelli

Education:

Ph.D. 1986 Cornell University, Ithaca, NY

Area of study: Plant Pathology, Genetics, Plant Breeding

M.S. 1978 University of California, Berkeley, CA

Area of study: Plant Pathology

B.S. 1973 Cornell University

Professional Experience:

USDA, ARS, Appalachian Fruit Research Station, Kearneysville, WV

Research Plant Pathologist (GS14), 2001-present

Cornell University, New York State Agricultural Experiment Station, Geneva

Senior Research Associate, 1993-2001

Research Associate, 1987-1993

Research Support Specialist, 1978-1986

Cornell University, Ithaca

Experimentalist, 1974-1976

Professional Activities:

·  Member of Executive Committee for U.S. Rosaceae Genomics, Genetics and Breeding Initiative 2005-2008, Secretary 2010, Vice-Chair/Chair elect 2011/2012

·  Member of Advisory Council for the Northeastern IPM Center, 2005-2010

·  Served as Technical Advisor to U.S. negotiators from the Office of the U.S. Trade Representative at the World Trade Organization (WTO) headquarters regarding WTO case ‘Japan-Measures Affecting the Importation of Apples (WT.DS245), Recourse by the United States to Article 21.5 of the DSU’, 2005

·  Served on the following committees of the American Phytopathological Society: Bacteriology (2004-present), Host Resistance (2002-2004), Biotechnology Impact Assessment (1998-2001, chair 1999-2000), officer of Potomac Division (Local Arrangements Chair 2003, Vice President 2004-05, President 2005-06)

·  Served on the following committees of the International Society for Horticultural Sciences: Scientific Committees for the 8th (1998), 9th (2001), 10th (2004), 11th (2007) and 12th (2010) Intl. Workshop on Fire Blight and for the 1st (2008) Intl. Symposium on Biotechnology of Fruit Species; Program Committees for 1st International Symposium on Transgenic Fruits (2005) and 4th International Workshop on Fire Blight (1986)

·  Organizer of the Fruit and Nut Workshop at Plant and Animal Genome XIX Conference, January 15-19, 2011, San Diego, CA

Honors and Awards Received:

·  Enhancing International Science Networks Visiting Fellowship, Department of Natural Resources and Environment, Victoria, Australia, sponsored 3 wk visit for scientific exchange and advising, 2002

·  Honorary Scientist of the Rural Development Administration, Republic of Korea, 1997 thru 2000

Grants Received (representative selection):

·  USDA-ARS Research Associate Program – Class of 2006 (Post Doctorial Program), “Characterize regulation of resistance/susceptibility responses of apple to fire blight”, $100,000, January 2006-December 2008

·  USDA-CRSEES-National Research Initiatives (Plant Genomics Program), “Functional Genomic Response of Apple to Fire Blight”, $445,000, September 2005-August 2009

·  Washington Tree Fruit Research Commission (grower commodity group):

o  “Identifying fire blight resistance in Malus sieversii for scion breeding”, $48,000, March 2010-February 2012

o  “Trait modification of conventional apple scions mediated by genetically engineered apple rootstocks”, $52,669, March 2004-February 2008

Research Interests: Functional and applied genomics aimed at improving stress and disease resistance in fruit trees. Emphasis is on fire blight disease of apple. Specific objectives include using genomic tools to characterize the global response of apple to fire blight disease and to identify molecular markers for fire blight resistance; developing basic knowledge of host-pathogen interactions in order to characterize the underlying basis for resistance and susceptibility in fruit trees; developing management strategies for fire blight based upon the use of resistance, cultural practices, growth regulators and natural compounds; and the genetic engineering of apple for enhanced resistance to abiotic and biotic stress.

Recent Accomplishments:

·  Characterize transcriptome of apple to fire blight disease by SSH, cDNA-AFLP and microarray (Norelli et al. 2009, Baldo et al. 2010, Bocsanczy, in prep; respectively)

·  Worked with a team of plant breeders, plant pathologists and horticulturalists to develop size-controlling apple rootstocks that are resistant to abiotic and biotic stress, including fire blight disease (see Publications: Norelli et al. 2003 Plant Disease 87:26-32).

·  Developed efficient transformation protocols for apple cultivars of economic importance including McIntosh, Gala, and M.26 rootstock, and studied the stability and expression of transgenes in transgenic apple.

·  Developed a management strategy for successfully using the plant growth regulator prohexadione-calcium (Phd-Ca) to increase fire blight resistance in young apple trees without excessive vegetative growth reduction (see Norelli and Miller 2004 Plant Disease 88:1099-1106).

Peer Reviewed Journal Articles:

Beer, S.V. and Norelli, J.L. Streptomycin-resistant Erwinia amylovora not found in western New York pear and apple orchards. Plant Disease Reporter 60:624-626. 1976.

Beer, S.V. and Norelli, J.L. Fire blight epidemiology: factors affecting release of Erwinia amylovora by cankers. Phytopathology 67:1119-1125. 1977.

Norelli, J.L. and Gilpatrick, J.D. Techniques for screening chemicals for fire blight control. Plant Disease 66:1162-1165. 1982.

Norelli, J.L., Aldwinckle, H.S. and Beer, S.V. Differential host X pathogen interactions among cultivars of apple and strains of Erwinia amylovora. Phytopathology 74:136-139. 1984.

Norelli, J.L., Aldwinckle, H.S. and Beer, S.V. Differential susceptibility of Malus spp. Robusta 5, Novole, and Ottawa 523 to infection by Erwinia amylovora. Plant Disease 70:1017-1019. 1986.

Norelli, J.L., Aldwinckle, H.S., Beer, S.V. and Lamb, R.C. Virulence of Erwinia amylovora affects the evaluation of fire blight resistance in Malus. Phytopathology 77:1551-1555. 1987.

Burr, T.J., Norelli, J.L., Katz, B., Wilcox, W.F. and Hoying, S.A. Streptomycin resistance of Pseudomonas syringae pv. papulans in apple orchards and its association with a conjugative plasmid. Phytopathology 78:410-413. 1988.

L., Aldwinckle, H.S. and Beer, S.V. Virulence of Erwinia amylovora strains to Malus sp. Novole plants grown in vitro and in the greenhouse. Phytopathology 78:1292-1297. 1988.

Burr, T.J., Norelli, J.L., Katz, B.H. and Bishop, A.L. Use of Ti plasmid DNA probes for determining tumorigenicity of Agrobacterium strains. Applied and Environmental Microbiology 56:1782-1785. 1990.

Jones, A.L., Norelli, J.L. and Ehret, G.R. Detection of streptomycin-resistant Pseudomonas syringae pv. papulans in Michigan apple orchards. Plant Disease 75:529-531. 1991.

Norelli, J.L., Burr, T.J., Lo Cicero, A.M., Gilbert, M.T. and Katz, B.H. Homologous streptomycin resistance gene present among diverse gram-negative bacteria in New York State apple orchards. Applied and Environmental Microbiology 57:486-491. 1991.

Burr, T.J., Norelli, J.L., Reid, C.L., Capron, L.K., Nelson, L.S., Aldwinckle, H.S. and Wilcox, W.F. Streptomycin-resistant bacteria associated with fire blight infections. Plant Disease 77:63-66. 1993.

Norelli, J.L. and Aldwinckle, H.S. The effect of aminoglycoside antibiotics on the regeneration and selection of neomycin phosphotransferase-transgenic apple plants and cells. Journal American Society for Horticultural Science 118:311-316. 1993.

Norelli, J.L., Mills, J.A. and Aldwinckle, H.S. Leaf wounding increases efficiency of Agrobacterium-mediated transformation of apple. HortScience 31:1026-1027. 1996.

Momol, M.T., Momol, E.A., Lamboy, W.F., Norelli, J.L., Beer, S.V. and Aldwinckle, H.S. Characterization of Erwinia amylovora strains using random amplified polymorphic DNA fragments (RAPDs). Journal of Applied Microbiology 82:389-398. 1997.

Aldwinckle, H. S. and Norelli, J. L. Transgenic pomaceous fruit with fire blight resistance. U.S. Patent 5,824,861. Issued October 20, 1998.

Norelli, J.L. and Aldwinckle, H.S. An efficient method for rooting and acclimation of micropropagated apple cultivars. HortScience 33:1251-1252. 1998.

Ko, K., Brown, S.K., Norelli, J.L. and Aldwinckle, H.S. Alternations in nptII and gus expression following micropropagation of transgenic M.7 apple rootstock lines. Journal American Society for Horticultural Science 123:11-18. 1998.

Momol, M.T., Norelli, J.L., Piccioni, D.E., Momol, E.A., Gustafson, H.L., Cummins, J.N. and Aldwinckle, H.S. Internal movement of Erwinia amylovora through symptomless apple scion tissues into the rootstock. Plant Disease 82:646-650. 1998.

Bolar, J.P., Brown, S.K., Norelli, J.L. and Aldwinckle, H.S. Factors affecting the transformation of 'Marshall McIntosh' apple by Agrobacteruim tumefaciens. Plant Cell, Tissue and Organ culture 55:31-38. 1999.

Ko, K., Norelli, J.L., Brown, S.K. and Aldwinckle, H.S. Anti-attacin polyclonal antibody from an in vitro derived antigen used for immunoblot to quantify attacin expressed in transgenic apple. Biotechnology Techniques 13:849-867. 1999.

Reynoird, J.P., Mourgues, F., Norelli, J., Aldwinckle, H.S., Brisset, M.N. and Chevreau, E. First evidence for improved resistance to fire blight in transgenic pear expressing the attacin E gene from Hyalophora cecropia. Plant Science 149:23-31. 1999.

Aldwinckle, H. S. and Norelli, J. L. Transgenic pomaceous fruit with fire blight resistance. U.S. Patent 6,100,453. Issued August 8, 2000.

Bolar, J.P., Norelli, J.L., Wong, K.-W., Hayes, C.K., Harman, G.E. and Aldwinckle, H.S. Expression of endochitinase from Trichoderma harzianum in transgenic apple increases resistance to apple scab and stunts growth. Phytopathology 90:72-77. 2000.

Ko, K., Norelli, J.L., Reynoird, J.-P., Boresjza-Wysocka, E., Brown, S.K. and Aldwinckle, H.S. Effect of untranslated leader sequence of AMV RNA 4 and signal peptide of pathogenesis-related protein 1b on attacin gene expression, and resistance to fire blight in transgenic apple. Biotechnology Letters 22:373-381. 2000.

Bessho, H., Brown, S.K., Norelli, J.L., Aldwinckle, H.S. and Cummins, J.N. Observations on the susceptibility of Japanese apple cultivars and rootstock selections to fire blight. Journal American Pomological Society 55:120-124. 2001.

Bolar, J.P., Norelli, J.L., Harman, G.E., Brown, S.K. and Aldwinckle, H.S. Synergistic activity of endochitinase and exochitinase from Trichoderma atroviride (T. harzianum) against pathogenic fungus Venturia inaequalis in transgenic apple plants. Transgenic Research 10:533-543. 2001.

Ko, K., Norelli, J.L., Reynoird, J.-P., Aldwinckle, H.S, and Brown, S.K. T4 lysozyme and attacin genes enhance resistance of transgenic ‘Galaxy’ apple against Erwinia amylovora. Journal American Society of Horticultural Science 127: 515-519. 2002.

Schupp, J.R., Rosenberger, D.A., Robinson, T.L., Aldwinckle, H.S., Norelli, J. and Propiglia, P.T. Post-symptom sprays of prohexadione-calcium affect fire blight infection of ‘Gala; apple on susceptible or resistant rootstocks. HortScience 37:903-905. 2002.

Norelli, J. L., Holleran, H. T., Johnson, W. C., Robinson, T. L. and Aldwinckle, H. S. Resistance of Geneva and other apple rootstocks to Erwinia amylovora. Plant Disease 87:26-32. 2003.

Norelli, J.L., Jones, A. L., and Aldwinckle, H. S. Fire blight management in the 21st century: using new technologies that enhance host resistance in apple. Plant Disease 87:756-765. 2003.

Wisniewski, M. E., Bassett, C. L., Artlip, T. S., Webb, R. P., Wojciech, J. J., Norelli, J. L., Goldway, M. and Droby S. Characterization of a defensin in bark and fruit tissues of peach and antimicrobial activity of a recombinant defensin in the yeast, Pichia pastoris. Physiologia Plantarum 119:563-572. 2003.

Norelli, J. L., and Miller, S. L. Effect of prohexadione-calcium dose level on shoot growth and fire blight in young apple trees. Plant Disease 88:1099-1106. 2004.

Bassett, C.L., Wisniewski, M.E., Artlip, T.S., Norelli, J.L., Renaut, J., and Farrell Jr., R.E. Global analysis of genes regulated by low temperature and photoperiod in peach bark. Journal American Society for Horticultural Science 131:551-563. 2006.

Wisniewski, M., Bassett, C., Norelli, J., Macarisin, D., Artlip, T., Kasenija Gasic, K., Korban, S. Expressed sequence tag analysis of the response of apple (Malus x domestica) to low temperature and water deficit. Physiologia Plantarum 133:298-317. 2008.

Norelli, J.L., R.E. Farrell, Jr., C.L. Bassett, A.M. Baldo, D.A. Lalli, H.S. Aldwinckle, and M.E. Wisniewski. Rapid transcriptional response of apple to fire blight disease revealed by cDNA suppression subtractive hybridization analysis. Tree Genetics and Genomes 5:27-40. 2009.

Bassett, C.L., Wisniewski, M.E., Artlip, T.S., Richart, G., Norelli, J.L., Farrell, Jr. R.E.

Comparative expression profiling and transcript initiation of three peach dehydrin genes. Planta 230:107-118. 2009.

Baldo, A.M., Norelli, J.L., Farrell, Jr. R.E., Bassett, C.L., Aldwinckle, H.S. and Malnoy, M. Identification of genes differentially expressed during interaction of resistant and susceptible apple cultivars (Malus x domestica) with Erwinia amylovora. BMC Plant Biology 10:1 (http://www.biomedcentral.com/1471-2229/10/1). 2010.

Boresjza-Wysocka, E.E., Norelli, J.L., Aldwinckle, H.S. and Malnoy, M. (co-senior author) Stable expression and phenotypic impact of attacin E transgene in orchard grown apple trees over a 12 year period. BMC Biotechnology 10:41 (http://www.biomedcentral.com/1472-6750/10/41). 2010.

Ko, K., Brown, S.K., Norelli, J.L., Hrazdina, G. and Aldwinckle, H.S. In vitro pollen functionality of attacin-transgenic ‘Royal Gala’ apple plants and apples transformed with 1-aminocyclopropane-1-carboxylic acid synthase (ACS)-antisense vector. Plant Biosystems 144:792-797. 2010.

Malnoy, M., Boresjza-Wysocka, E.E., Norelli, J.L., Flaishman, M.A., Gidoni, D. and Aldwinckle, H. Genetic transformation of apple (Malus X domestica) without use of a selectable marker gene. Tree Genetics and Genomes 6:423-433. 2010.

Wisniewski, M., Norelli, J., Bassett, C., Artlip, T. and Macarisin D. Ectopic expression of a novel peach (Prunus persica) CBF transcription factor in apple (Malus x domestica) results in short-day induced dormancy and increased cold hardiness. Planta 233:971-983. 2011.

Liu, J., Macarisin, D., Wisniewski, M., Droby, S., Norelli, J. and Hershkovitz, V. Production of hydrogen peroxide and expression of ROS-generating genes in peach flower petals in response to host and non-host fungal pathogens. Plant Pathology DOI: 10.1111/j.1365-3059.2012.02683. 2012.

Liu, J., Sui, Y., Wisniewski, M., Droby, S., Tian, S., Norelli, J. and Hershkovitz, V. Effect of heat treatment on inhibition of Monilinia fructicola and induction of disease resistance in peach fruit. Postharvest Biology and Technology 65:61-68. 2012.

Liu, J., Wisniewski, M., Droby, S., Norelli, J., Hershkovitz, V., Tian, S. and Farrell, R. Increase in antioxidant gene transcripts, stress tolerance and biocontrol efficacy of Candida oleophila following sublethal oxidative stress exposure. FEMS Microbiology Ecology 80:578-590. 2012.

Gardiner, S.E., Norelli, J.L. (co-senior author), de Silva, N., Fazio, G., Peil, A., Malnoy, M., Horner, M., Bowatte, D., Carlisle, C., Wiedow, C., Wan, Y.; Bassett, C.L., Baldo, A.M., Celton, J.M., Richter, K., Aldwinckle, H.S. and Bus, V.G. Putative resistance gene markers associated with quantitative trait loci for fire blight resistance in Malus 'Robusta 5' accessions. BMC Genetics 13 Article No.: 25 (http://www.biomedcentral.com/content/pdf/1471-2156-13-25.pdf). 2012.

Sui, Y., Liu, J., Wisniewski, M., Droby, S., Norelli, J. and Hershkovitz, V. Pretreatment of the yeast antagonist, Candida oleophila, with glycine betaine increases oxidative stress tolerance in the microenvironment of apple wounds. International Journal of Food Microbiology 157:45-51. 2012.

Aldwinckle, H.S. and Norelli, J.L. Varietal differences in the incidence of infection of apple blossoms and shoots by Erwinia amylovora (fire blight). Acta Horticulturae 117:71-72. 1981. (Proceedings of 2nd Int. Workshop on Fire Blight, Kiel-Schilksee, Germany, 1980)

Beer, S.V., Rundle, J.R. and Norelli, J.L. Biological control of fire blight by Erwinia herbicola. pp. 596-597 In: Lozano, J.C. (ed.) 5th Int. Conf. Plant Path. Bac., Cali, Columbia, 1981. (Proceedings)