Table S4: Quantitative Proteomic Analysis

Table S4: Quantitative proteomic analysis

Gene / Protein / Protein description / 176 wt (ng) / 176ΔenvC (ng)
Lipoproteins / NTHI1957
NTHI0267
NTHI0353
NTHI0363
NTHI0830
NTHI0252
NTHI1473
NTHI1005
NTHI0816
NTHI0501
NTHI0509
NTHI0576
NTHI0266
NTHI1140 / YP_249369.1
YP_247895.1
YP_247966.1
YP_247975.1
YP_248392.1
YP_247882.1
YP_248948.1
YP_248545.1
YP_248382.1
YP_248096.1
YP_248104.1
YP_248161.1
YP_247894.1
YP_248666.1 / putative lipoprotein
hypothetical protein
hypothetical protein
lipoprotein
lipoprotein
lipoprotein
outer membrane lipoprotein PCP
small protein A
outer membrane protein P4 NADP phosphatase
outer membrane protein P6
starvation inducible outer membrane lipoprotein
hypothetical protein
hypothetical protein
hypothetical protein / 2.79
0.23
1.53
0.34
0.33
0.17
0.17
ND
ND
0.74
0.17
0.47
0.70
0.46 / 2.57
ND
1.42
0.27
0.42
0.13
0.12
0.05
0.28
0.82
0.07
0.17
0.57
1.57
β-barrel containing OMP / NTHI0225
NTHI1332
NTHI1390
NTHI1449
NTHI1084
NTHI0522
NTHI1668
NTHI1342 / YP_247859.1
YP_248824.1
YP_248875.1
YP_248928.1
YP_248615.1
YP_248116.1
YP_249121.1
YP_248834.1 / outer membrane protein P2
outer membrane protein P5
heme utilization protein
HMW2B OMP 85 like protein required for HMW1A and HMW2A secretion
protective surface antigen D15
long chain fatty acid ABC transporter
hypothetical protein NTHI1668
opacity protein / 40.23
16.69
0.73
1.19
1.43
0.65
0.30
0.47 / 33.67
23.52
1.07
0.85
1.53
1.67
0.42
1.11
Auto
transporters / NTHI0354
NTHI1450
NTHI0371
NTHI1164 / YP_247967.1
YP_248929.1
YP_247983.1
YP_248687.1 / Hap adhesion and penetration protein
HMW2A
heme hemopexin binding protein A
IgA specific serine endopeptidase / ND
ND
ND
ND / PMF
PMF
0.70
9.54
Periplasmic proteins / NTHI0303
NTHI1208
NTHI1206
NTHI1774
NTHI2000
NTHI0481
NTHI1083
NTHI1905
NTHI0588
NTHI0707
NTHI1292
NTHI1207
NTHI0177
NTHI0208
NTHI0232
NTHI0235
NTHI0626
NTHI0623
NTHI0632
NTHI1243
NTHI2017
NTHI1960
NTHI1384
NTHI0716
NTHI0741
NTHI0987
NTHI1012
NTHI1247 / YP_247923.1
YP_248722.1
YP_248720.1
YP_249212.1
YP_249409.1
YP_248077.1
YP_248614.1
YP_249323.1
YP_248172.1
YP_248286.1
YP_248787.1
YP_248721.1
YP_247820.1
YP_247844.1
YP_247866.1
YP_247868.1
YP_248209.1
YP_248206.1
YP_248215.1
YP_248749.1
YP_249424.1
YP_249372.1
YP_248871.1
YP_248293.1
YP_248315.1
YP_248529.1
YP_248552.1
YP_248753.1 / NAD nucleotidase
hypothetical protein
anaerobic dimethyl sulfoxide reductase chain B
phosphate binding periplasmic protein PstS
molybdate binding periplasmic protein
putative periplasmic chelated iron binding protein
outer membrane protein 26
periplasmic serine protease do HhoA like
survival protein SurA like protein
FKBP type peptidyl prolyl cis trans isomerase
periplasmic oligopeptide binding protein
anaerobic dimethyl sulfoxide reductase chain A
iron utilization periplasmic protein hFbpA
high affinity zinc transporter periplasmic component
putative sialic acid transporter TRAP type C4 dicarboxylate - periplasmic comp
N acetylneuraminic acid mutarotase
spermidine putrescine binding periplasmic protein 2
acid phosphatase phosphotransferase
D ribose transporter subunit RbsB
amino acid ABC transporter binding protein
hypothetical protein
hypothetical protein
thiol disulfide interchange protein
periplasmic negative regulator of sigmaE
bifunctional 2 3 cyclic nucleotide 2 phosphodiesterase 3 nucleotidase
D galactose binding periplasmic protein
thiol disulfide interchange protein DsbA
ABC transporter / 1.06
0.47
0.24
0.76
0.29
1.85
3.44
8.28
0.55
1.23
1.87
1.19
0.24
0.28
0.59
0.39
0.18
0.26
0.32
0.13
0.11
0.25
0.47
0.28
2.04
2.56
0.50
0.13 / 0.68
ND
0.06
0.88
0.17
1.33
2.20
1.56
0.35
0.64
0.63
0.59
0.20
0.22
0.36
ND
ND
0.12
0.07
ND
ND
0.13
0.30
0.15
1.30
2.06
0.32
0.12
Cytoplasmic proteins / NTHI0669
NTHI0748
NTHI2020 / YP_248252.1
YP_248322.1
YP_249425.1 / chaperonin GroEL
elongation factor Tu
PTS system glucose specific transporter subunit / ND
ND
ND / 0.54
0.27
10.66
Unknown by software prediction / NTHI0502
NTHI1418
NTHI1987 / YP_248097.1
YP_248898.1
YP_249397.1 / translocation protein TolB
putative ABC type Co2 transport system periplasmic component
hypothetical protein / 0.78
0.59
0.32 / 1.16
0.91
0.48

ND: not detectable. PMF: identified by peptide mass fingerprinting