MCB 7300: MOLECULAR AND CELLULAR BIOLOGY LABORATORY
(Class Number4063 – 3.0 Credit Hours)-Spring 2013
Time & Place: Mon. & Wed. 3:05 PM –6:05 PM in the locations listed on the syllabus.
Course Coordinator: Allan Showalter
Instructors: MCB Faculty as listed on the syllabus
Text: None. Handouts and/or assigned readings will be provided by the instructors.
Requirements and Course Description:
Prerequisites: MCB 7200 or permission. The purpose of this course is to expose students to a variety of laboratory techniques used in the broad field of molecular and cellular biology by allowing the student to carry out these techniques in the laboratory.
Grades will be based on the following:
1.Each instructor, with the exceptions of Rowe,Miller, and King, will evaluate the student based on a 100 point scale (or 200 points in the case of the Brijulab and 300 points in the case of the Nadella lab) using one or more of the following criteria: 1) a written lab report, 2) a laboratory examination, and 3) instructor observation of student preparation and participation in the lab.
2.Each instructor, with the exceptions listed above, will submit student scores to the course coordinator who will total these scores and record the final grade of the student based on a total of 1300 possible points. (Note that the Briju and Nadella labs will be worth 200 and 300 points respectively, while the other labs will be worth 100 points each). A standard percentage grading system will be used. Thus, 93.3% and above will earn an A, 90-93.3% an A-, 86.7-90% a B+, 83.3-86.7% a B, 80-83.3% a B-, 76.7-80% a C+, 73.3-76.7% a C, 70-73.3% a C-, 66.7-70% a D+, 63.3-66.7% a D, 60-63.3% a D-, and below 60% an F.
Office Hours:
Allan Showalter- By appointment, Porter 504, phone 593-1135.
Email:
Academic Conduct:
The penalty for course-related academic dishonesty (i.e., plagiarism, cheating, etc.) will be failure of the entire course.
Attendance Policy:
Attendance for laboratory is mandatory. All absences must be justified and explained to the instructor and the course coordinator in advance. Each unjustified absence will result in the reduction of the student's final course grade by one full letter grade.
Course web page:
MCB 7300: MOLECULAR AND CELLULAR BIOLOGY LABORATORY
Syllabus - Spring 2013
Instructors: MCB Faculty as listed below. Please take special note where each lab is to be held.
WEEKDATELaboratory (Instructor-lab location)
1Jan. 14Genomic DNA isolation, finding a gene of interest (Briju -Porter 304)
Jan. 16Electrophoresis, ligation & transformation (Briju -Porter 304)
2Jan. 21Martin Luther King Day (no class)
Jan. 23Blue-white screening and plasmid prep (Briju -Porter 304)
3Jan. 28Restriction digestion and gel electrophoresis (Briju -Porter 304)
Jan. 30Introduction to DNA sequencing/ PCR (Nadella-Porter 304)
4Feb. 4DNA sequence analysis/Fragment analysis Lab I (Nadella-Porter 304)
Feb. 6Fragment analysis Lab II/ RNA extraction (Nadella- Porter 304)
5Feb. 11Intro to qPCR, RNA quality, qPCR setup (Nadella-Porter 304)
Feb. 13QPCR analysis; Introduction to microarrays (Nadella-Porter 304)
6Feb. 18Next gen sequencing I(Nadella-Porter 304)
Feb. 20Next gen sequencing II(Nadella-Porter 304)
7Feb. 25Next gen sequencing III (Nadella-Porter 304)
Feb. 27Bioinformatics I/Vector NTI & CLC Genomics (Charlier-Porter 304)
-March 4Sprink Break (no class)
March 6Sprink Break (no class)
8March 11Bioinformatics II/ Vector NTI & CLC Genomics (Charlier-Porter 304)
March 13Diagnostic Hybrids Field Trip (Kevin King-meet in Porter 304)
9March 18Genetic Pathway I/Southern blot analysis I (Showalter-Porter 304)
March 20Genetic Pathway II/Southern blot analysis II (Showalter-Porter 304)
10March 25Western blot analysis I (McCall-Porter 304)
March 27Western blot analysis II (McCall-Porter 304)
11April 1Laser capture dissection microscopy (Showalter/Plant-Porter 304)
April 3Laser capture dissection microscopy (Showalter/Plant-Porter 304)
12April 8Fluorescence microscopy lab I (Berryman-Porter 304)
April 10Fluorescence microscopy lab II (Duerr-Porter 305)
13April 15EM Lab IA (Miller- Wilson 014)/Confocal Lab IIB (Rowe-Wilson 014)
April 17Confocal Lab IA (Rowe-Wilson 014)/EM Lab IIB (Miller- Wilson 014)
14April 22Beta-galactosidase assay lab (Coschigano-Porter 304)
April 24Mammalian transfection lab (Wu-Porter 304)
May 1Final Exam, 12:20 PM (lab evaluations—no final exam)
Teaching assistants:Debarti Basu-email:
Xiao Liu- email: