1
Biology (Mwit’14)
Research Title The Effectiveness of Terminalia catappaLeaveson Bubble Nest Production
of Siamese Fighting Fish
ResearchersMiss Kittiya Paungpitikul,Miss Thanutra Zhang,Miss Thummaporn Boonvisudhi
AdvisorsMiss Sataporn Wantanawijarn
Department Biology
School Mahidol Wittayanusorn Academic Year2006
Abstract
The study examined the effectiveness of Terminalia catappa leaves on the production of bubble nest of Siamese fighting fish. Three kinds of water were set; 1) water dipped with Terminalia catappa leaves 2) water boiled with Terminalia catappa leavesand 3) clean pipe water. The Siamese fighting fish were fed in each water kind. The results showed that those fed in water dipped with Terminalia catappa leaves produced the largest area of bubble nests which were 61.22 square centimeters; whereas those fed in water boiled with Terminalia catappa leaves and clean pipe water produced 31.51 and 12.45 square centimeters of bubble nests, respectively. The fish in water dipped and boiled with Terminalia catappa leaves produced longer bubble nests than those in clean pipe water. Furthermore, the bubble nests in water dipped and boiled with Terminalia catappa leaves stayed longer than those in clean pipe water. The sizes of bubble nests in three water kinds were not different.
Research Title A Study of Foods Effect on Planarian Populations Dugesia sp.
ResearchersMiss Patrawee Tangprasert, Miss Sirikorn Pamonsupornvichitand
Miss Wichurat Sakulpaptong
AdvisorsMiss Woranis Klintong, Mr. Bancha Sabaitua, Miss Messuwan Pongpramoon, Mr. Sakchai Kamarangkoon, and Miss Tulaporn Buranasomphop
Department Biology
School Mahidol Wittayanusorn Academic Year2006
Abstract
This study identifies types of food which result in the proliferation of planarians Dugesia sp. Planarians were separated into 4 groups; red-worm-fed, water-flea-fed, chicken-liver-fed, and boiled-egg-yolk-fed. Planarians were fed 2 times a week over a period of 4 weeks, and then they were counted. After conducting the experiment 15 times for accuracy, the results showed that the amount of water-flea-fed planarians increased more than red-worm-fed, chicken-liver-fed, and egg-yolk fed planarians, respectively. However, the body size-structure of chicken-liver-fed planarians increased more than red-worm-fed, egg-yolk-fed, and water-flea-fed planarians, respectively. The pH of planarian water used with planarians was measured and found that the water-flea-fed water had an average pH of 8.6, while the boiled-egg yolk feeding water had an average pH of only 7.9. The pH result indicated that the optimum pH of water for feeding planarians is around 8.3-8.6. In conclusion, sorts of food which produce this pH range are best for fostering the growth of planarians.
Research Title The Relationship between the Hemispheric Dominance and 9 Human abilities of M.4 and M.5 Mahidol Wittayanusorn School Academic Year 2006 Students
ResearchersMr. Phakhawet Techatewon andMr. Marut Laohaviroj
AdvisorsMiss Montakan Subkaew, Miss Tanyaratana Dumkua, Miss Tawashini Rojanavi and Assistant ProfessorPh.D. Usanee Anurutwong
Department Biology
School Mahidol Wittayanusorn Academic Year2006
Abstract
The comparison of the exploration of the hemispheric dominance by using the test created by the researchers and by Assistant Professor Dr. Usanee created had the same result at 54.33%. When comparing the tests by allowed 2 points for errors, the tests has the same result of 64.72%. When using the researchers test and the 9 human abilities test, There are more percentage of students who are left hemispheric dominant and have Mathematical abilities than right hemispheric dominant students. But there are right hemispheric dominant students who have Bodily ability, Visual ability, Linguistic ability and Emotional ability more than left hemispheric dominant students. When analyzed by using the Pearson’s correlation, almost all of the human relate to the others, especially in both hemispheric dominant students. When analyzed by using the multiple regression using all students and divided students into groups according to their sex and educational level. The result shows that sex is related to the hemispheric dominance because the male students has multiple R-squaredmore than all students 2.3%, the female students has multiple R-squaredmore than all students 5.5%, the M.5 students has multiple R-squaredmore than all students 0.8% and the M.4 students has multiple R-squaredmore than all students 1.5%. In conclusion, the Mathematical ability influences students to be left hemispheric dominant; moreover, the Mechanical ability, Technical ability and Auditory ability influence students to be right hemispheric dominant and the others depend on valuables that use for dividing. Thus, the relationship between the hemispheric dominance and 9 human abilities also depend on other factors and each of 9 human abilities relate to having one the other abilities.
Research Title Effect of Temperatures and Ultraviolet Radiation on Hatchability Rate of Yellow Fever Mosquito’s eggs (Aedes aegypti)
ResearchersMissDudrudee Chaiittiporn, Mr.Parin Jirapatrasilpand Mr.Pakorn Aiewsakul
AdvisorsMissSataporn Wantanawijarn,MissTanyaratana Dumkua and Mr.Winyoo Phanmuangma
Department Biology
School Mahidol Wittayanusorn Academic Year2006
Abstract
Environment is one of the factors affecting the survivorship of the living things, including mosquitoes’ hatchability. Therefore, the changes of physical factors would affect the hatchability. In the studying of effect of temperatures on hatchability rate of Aedes aegypti’s eggs, the eggs were heated for 15 and 30 minutes, with the different temperatures: 35, 40, 45, 50 and 55oC.There were no statistical difference of the hatchability rate between eggs heated for 15 and 30 minutes.By further experiment of comparing the results of different temperatures on the hatchability rate, the eggs heated for 30 minutes with 35, 40, 45, and 50oC had the rate more than 0 percent, but the relation between temperatures and hatchability rates could not be concluded definitely. The eggs heated with the temperature of 55oChad the rate of 0 percent. In addition, by the studying of effect of Ultraviolet radiation on hatchability rate of the mosquitoes’ eggs, UVA affected the rate more than UVC. The hatchability rates of the eggs exposed to Ultraviolet for 15, 30 and 60 minutes trended to decrease but stayed above 0 percent.
Research Title The Astaxanthin extract affect the survival rate of Tub Tim fish
Researchers Mrs. Supakan Jiranapakul Mr. Chaipat Treeratsakulchai and
Mr.Pongpat Metapipattanakul
Advisors Ms. Orawan Piyaboon Ms. Sattaporn Wantanawijarn and
Ms. Tanyaratana Dumkua
Department Biology
School Mahidol WittayanusornAcademic Year 2006
Abstract
Nowadays the technology of feeding Tub Tim fish is not good enough. This causes the high death rate of Tub Tim fish. Recent years ago, it was found that astaxanthin, found in Haematococcus pluvialisalgae, affects the survival rate of the fish. This experiment was conducted by culturing Haematococcus pluvialis algae and extracting astaxanthin by acetone, non-polar solution which can dissolve non-polar substance named astaxanthin. Next, mix the extracted solution with fish food and feed the fish of age 1-2 months old and 2-3 months old. When compare to the control group in which the fish are fed with no astaxanthin, it can be found that astaxanthin affects 1-to-2-month-old Tub Tim fish more extremely than on 2-to-3-month-old Tub Tim fish. This is resulted from the low immunity that 1-to-2-month-old Tub Tim fish have. Hence, astaxanthin helps increase the fish’s immunity by acting as antioxidant. However, 2-3 month-old Tub Tim fish are stronger than 1-2 month-old Tub Tim fish.
Research Title Studying the Efficiency of Weeds in Wetland Wastewater Treatment
ResearchersMiss Waraporn Anuparp u-krit, Miss Thanchanok Ratvijitvectand
MissPaleerat Wattana u-dom
AdvisorsMr. Bancha Sabuytua,MissTanyaratana Dumkua, and Miss somruthai Homchuen
Department Biology
School Mahidol Wittayanusorn Academic Year2006
Abstract
The purposes of this study were to investigate the efficiency in wastewater treatment of three kinds of weeds: Eichhornia crassipes, Solms[Water Hyacinth], Cynodon plectostachyus [Star grass] and Ruellia tuberosa L. [Solanum spiral] in constructed wetlands for 4 days. The efficiency was evaluated by comparing color, odor, pH, DO and BOD of influent wastewater with effluent. Unit with Water Hyacinth was the best performance for wastewater treatment which could be the most decreasing pH from 7.71 to 7.50, increasing DO to 1.21 mg/l and decreasing BOD to 51.5 mg/l
Research Title The study on effect of crude extract from Andrographis paniculata to
the growth of Erwinia carotovora subspecies carotovora
ResearchersMr. Witchanon Dontisanor Mr. Suttipong Thongsuwan and
Mr. Wuttapon Sadaeng
AdvisorsMiss Orawan Piyaboon Miss Tanyaratana Dumkua and
Miss Somruthai Homcheun
DepartmentBiology
SchoolMahidol WittayanusornAcademic Year 2006
Abstract
One of important herbal plant, which is used for inhibiting microbial growth, is Andrographis paniculata. Nowadays, crude extract from A. paniculata was used in many researches for inhibiting microbial. Two types of A. paniculata were used for extracting in order to inhibiting Erwinia carotovora subspecies carotovora growth. Fresh aerial part and dried aerial part were extracted by three types of solvents; distilled water, ethanol 95% and acetone; correspondingly, 6 crude extracts were prepared. They were tested with E. carotovora at concentration 0.265% 2.5% 5% 20% 25% 50% 75% (w/v) by Bauer – Kirby Method (paper disk diffusion). Testing result showed that all crude extracts at various concentrations could not inhibit the growth of E. carotovora, however there are many researches supported that the crude extract from A. paniculata could inhibit Escherichia coli which is in the same family as E. carotovora. In order that, the ability of receptors of each bacteria were different from each other or the crude extract from A. paniculata from this extraction decomposed before using.
Research TitleThe Study of Circinate Movement and Relation Between Length, Weight and Number of Segments of Thyropygus allevatus
ResearchersMr. Soravit Changpinyo, Mr. Chumsaeng Chumsaengsriand Miss.Nattaporn Setasatian
AdvisorsMiss Orawan Piyaboon, Miss Tanyaratana Dumkoh,
Miss Sataporn Wantanawijarn, and Miss Amporn Boonyasatitsataporn
Department Biology
School Mahidol Wittayanusorn Academic Year2006
Abstract
The study of circinate movement and relation between length, weight and number of segments of Thyropygus allevatus was found that the best stimulated position is the head segment, the stimulated position and the time they used curling are not significantly relative. Their behavior, stimulating with pendulum, is considered learning behavior called habituation. In the first stage, millipedes responded to the stimulus longer. Then, they did less, no circinate movement when stimulated.
It was also founded that the average length, weight, and number of segments were 126.7608 mm, 13.8447 g, and 51.8333, respectively. Their big size and large number of segments could be considered a dead phase of growth. Moreover, length and number of segments were normally distributed [k-s test]. Millipede’s weight correlated to its length and number of segments with r = 0.8060 at p < 0.01. The weight prediction equation is W = 12.084 – 0.391N + 0.173L (W is weight [g]; N is number of segments; L is length [mm])
Research Title Ability of Turmeric Extract for Inhibiting Growth of Erwinia carotovara supsp. carotovora, the Causal Agent of Soft Rot
ResearchersMs. Pilaipan
AdvisorsMs.Orawan Piyaboon, Ms. Sataporn WantanawijarnandMs. Tanarat Dumkoh
Department Biology
School Mahidol Wittayanusorn Academic Year2006
Abstract
Efficacy test of turmeric extract for inhibiting growth of Erwinia carotovora supsp. carotovora, the causal agent of soft rot was conducted. In the experiment process, Erwinia carotovora supsp. carotovora were culture on double layer nutrient agar (double layer NA), then, the extracted substances that were extract by using 95% ethyl alcohol and varied in five level, 750 mg/ml, 500 mg/ml, 250 mg/ml, 100 mg/ml and 50 mg/ml would be brought in testing method aiming to evaluate the rate of bacterial inhibition efficiency. As the experimented, turmeric extract, extracted by using 95% ethyl alcohol, was tested on growth inhibition of Erwinia carotovora supsp. carotovora, could not show inhibition zone on bacteria culturing in double layer NA.
Research Title ComparativeStudy in Anatomy of Parkia speciosa and Parkia timoriana
Researcher Ms.Intuorn anupantanan and Ms.Kanokwan Limmangkun
Advisers Ms.Somruthai Homcheun ,Ms.Metsuwan Pongpramun, and
Mr.Win-yu Panmoungma
Department Biology
School Mahidol Wittayanusorn Academic 2006
Abstract
Comparative study in anatomy of Parkia speciosa and Parkia timoriana focused on root, stem and leaf. Paraffin method is used to make slides of the plants’tissues.The researchers found that in primary root of Parkia speciosa , there are 7 arches of xylem. In contrast , Parkia timoriana have 5 arches of xylem. In secondary root , while Parkia speciosa has sclerenchyma , there is no sclerenchyma in Parkia timoriana. In primary stem , Parkia speciosa has 1 layer of epidermis. In contrast , Parkia timoriana has 2 layer of epidermis. In secondary stem , Parkia timoriana has the smaller circle of pith with phloem sclerenchyma next to periderm. In leaf , both are bifacial which consist of palisade parenchyma and spongy parenchyma. In the apical meristem , both share the same type and tissue arrangement.
Research Title Comparative Anatomy of Root, Stem, Leave and Petiole of Variegatum
(Piper sarmentosum Roxb.) and Long Pepper (Piper chaba Hunt.)
ResearchersMr. Kitti Wuthisathid, Mr. Jira Jindalertudomdee and
Mr. Panchnok Srinualnat
AdvisorsMiss.Somruthai Homchin, Mrs. Sataporn Wantanawijarn and
Mr. Sarawut Sangurai
Department Biology
School Mahidol Wittayanusorn Academic Year2006
Abstract
In a study of the comparative anatomy ofroot, stem, leave and petiole of Piper sarmentosum Roxb. and Piper chaba Hunt. by using Paraffin Technique. It was found that there are many different characteristics. For example, in P. sarmentosum the trichome exists in the vegetative organ of all samples and the blade has a subepidermic layer in abaxial and adaxial face epidermis, . InP. chabano trichome exists, the blade presents a uniseriate epidermis and asubepidermic layer in theabaxial face. TheP. sarmentosum chlorenchyma isnext to epidermis in the stem and that of P.chabais separated from the epidermis.TheP. sarmentosumsclerenchyma isnear by phloem and that of P.chabais near by xylem. On the other hand, two species show some structural likenesses in that both have polyarch in root, one or two concentric rings of collateral bundle in stem and one open bundle in leaf and petiole.
Research Title A Search for the γ -Tocopherol Methyltransferase Production Gene in Five Thai
Fruit Plants
Researcher Mr. Manuswin Chansakulporn
Advisors Miss Tanyaratana Dumkua, Miss Somrutai Homchuen, Miss Woraya Kwaiphan,
Mr. Sakchai Kammarangkool and Dr. Supatcharee Netrphan
Department Biology
SchoolMahidol WittayanusornAcademic Year2006
Abstract
α-Tocopherol, a structure of Vitamin E, is an antioxidant in human body thus it helps many systems to work properly. In α-Tocopherol biosynthesis, the last enzyme that is necessary for the plant to synthesize is γ -Tocopherol methyltransferase (γ -TMT). In Thailand, the deficiency of Vitamin E is found in elder people, especially in the northeastern region where there is a very high level of the deficiency. The study about the ability of synthesizing Vitamin E in Thai fruit plants is a way to reduce that problem. The α-Tocopherol synthesis was determined by using molecular procedure to search for γ -TMT production gene in five Thai fruit plants that were Guava, Mango, Rambutan, Spanish plum and Tamarind. From the experiment, Guava was the only one Thai fruit plant that did not have this gene. After having searched for Nucleotide sequence of γ -Tocopherol gene in other plants, it had the fractions of intron squeeze through this gene. The average of percent similarity of other Thai fruit plantswas 78.1 percent except Mango that has only 37.4 percent, when compared with soybean’s γ -TMT cDNA sequence. Rambutan one of two sequences had similarly high percentage to Tamarind that is 99.9 percent when compared with each other. From the Phylogenetic tree, it was possible that one γ -TMT gene of Rambutan and γ -TMT gene of Tamarind had very close development. However, this research will have a complete conclusion in case I have some data of the nutrients in these Thai fruit plants, but such a study in Thai fruit plants is very few so I can only conclude that Mango, Rambutan, Spanish plum and Tamarind have an ability to synthesize α-Tocopherol that is very useful for human body.
Research TitleBiocontrol of Cutworms (Spodoptera litura) Using Entomopathogenic Fungi Beauveria bassiana
ResearchersMr. Chin Suppapinyaroj, Mr. Paramet Kongpitak and Mr. Sorakrit Atcharanuwat
AdvisorsMiss Orawan Piyaboon Miss Thanyaratana Dumku Miss Woranitt Klinthong and Dr. Tipwadee Attathom
DepartmentBiology
SchoolMahidol WittayanusornAcademic Year 2006
Abstract
The cutworm, Spodoptera litura, is one of the pests that cannot be easily controlled. To eliminate caterpillars by using insecticide, more pollute will be accumulated in the environment. In the research, it shows that Beauveria bassiana is one of the fungi which are very specific to insects and harmless to humanity – the most advantage characteristic to people. In the experiment, it was divided into replications, which were sprayed with fungi’s spores at various concentrations (1x106 1x107and 1x108 spores/ml). The result would be recorded within 7 days and analyzed. It was founded that the efficiency of the fungi suspension at 1x106 1x107and 1x108 spores/ml could kill the caterpillars at 47.76%, 71.1% and70%respectively. Furthermore, It showed that fungi suspension could kill the caterpillars more than 50% in the 1x107spores/ml and1x108spores/mlexperiment (P = 0.019 andP = 0.035). Conclusively, the least concentration of Beauveria bassiana suspension that could kill the caterpillars efficiently was 1x107 spores/ml.