Supplemental Table 1: Metabolite level in wild type and lap3-2 anthers determined by UPLC/TOFMS analysis. Data are normalized to the mean response calculated for the wild-type (WT). Values are presented as the mean ± RSD of three independent determinations. RSD relative standard deviation in %, ppm parts per million, RT retention time, n.d. not detected

PHENYLPROPANOIDS
WT / RSD / lap3/Wt / RSD / p-value / RT / observed ion [M-H]- / natural ion / proposed formula / proposed annotation / proposed natural mass / ppm
1.00 / ± / 16.45 / 0.81095236 / ± / 18.94 / 0.22 / 4.68 / 445.0777 / 446.0850 / C21H18O11 / 5,7,2'-Trihydroxyflavone 7-glucuronide / 446.0849114 / 0.20
1.00 / ± / 3.25 / n.d. / ± / 6.03 / 431.0868 / 432.0941 / C21H20O10 / Apigenin-6-C-glucoside / 432.1056469 / -26.72
1.00 / ± / 2.59 / 1.085001603 / ± / 9.75 / 0.25 / 5.65 / 445.0766 / 446.0839 / C21H18O11 / Baicalein 7-O-glucuronide / 446.085 / -2.24
1.00 / ± / 19.56 / 0.661968263 / ± / 37.39 / 0.14 / 7.06 / 477.1016 / 478.1089 / C22H22O12 / isoscoparine / 478.1111262 / -4.66
1.00 / ± / 20.95 / 0.8797514 / ± / 18.38 / 0.48 / 6.94 / 607.1639 / 608.1712 / C28H32O15 / Kaempferide 3-glucoside-7-rhamnoside / 608.1741 / -4.77
1.00 / ± / 21.98 / 0.76292683 / ± / 11.10 / 0.16 / 5.57 / 593.1509 / 594.1582 / C27H30O15 / kaempferol 3-galactoside-7-rhamnoside / 594.1585 / -0.50
1.00 / ± / 19.28 / 0.772230402 / ± / 5.93 / 0.12 / 3.93 / 755.2072 / 756.2145 / C36H36O18 / kaempferol 3-glucoside-7-p-coumarylglucoside / 756.1901 / 32.27
1.00 / ± / 17.39 / 0.812023491 / ± / 4.08 / 0.14 / 6.76 / 607.1289 / 608.1362 / C27H28O16 / Kaempferol 3-rhamnoside-7-galacturonide / 608.1377349 / -2.52
1.00 / ± / 25.64 / 0.733434929 / ± / 9.84 / 0.16 / 5.97 / 623.1618 / 624.1691 / C28H32O16 / Kaempferol 5-methyl ether 3-galactoside-4'-glucoside / 624.169 / 0.16
1.00 / ± / 4.83 / 1.36438265 / ± / 31.18 / 0.21 / 6.47 / 577.1563 / 578.1636 / C27H30O14 / kaempferol-3-rhamnoside-7-rhamnoside / 578.1635557 / 0.08
1.00 / ± / 39.14 / 0.64255582 / ± / 16.25 / 0.20 / 6.20 / 593.1530 / 594.1603 / C27H30O15 / Luteolin-7-O-rhamnoside; / 594.1584703 / 3.08
1.00 / ± / 52.64 / 0.489563432 / ± / 97.66 / 0.35 / 9.99 / 271.0622 / 272.0695 / C15H12O5 / Naringenin / 272.0684735 / 3.77
1.00 / ± / 72.00 / n.d. / ± / 10.50 / 271.0591 / 272.0664 / C15H12O5 / Naringenin chalcone / 272.0684735 / -7.62
1.00 / ± / 43.49 / 0.531883372 / ± / 5.30 / 0.14 / 4.83 / 609.1442 / 610.1515 / C27H30O16 / quercetin 3-glucoside-7-rhamnoside / 610.1534 / -3.11
1.00 / ± / 21.71 / 0.863773538 / ± / 16.18 / 0.41 / 4.44 / 739.2111 / 740.2184 / C33H40O19 / Robinin / 740.2163 / 2.84
1.00 / ± / 3.00 / 1.005641 / ± / 9.15 / 0.92 / 0.76 / 333.0589 / 334.0662 / C16H14O8 / 6-Methoxytaxifolin / 334.0688674 / -7.98
GLUCOSINOLATE
WT / RSD / lap3/Wt / RSD / p-value / RT / observed ion [M-H]- / natural ion / proposed formula / proposed annotation / proposed natural mass / ppm
1.00 / ± / 27.17 / 1.625844597 / ± / 8.78 / 0.02 / 0.74 / 376.0378 / 377.0451 / C10H19NO10S2 / 3-hydroxypropyl-glucosinolate / 377.045 / 0.27
1.00 / ± / 36.73 / 0.707748238 / ± / 1.21 / 0.24 / 2.83 / 492.1040 / 493.1113 / C16H31NO10S3 / 8-Methylsulfinyloctyl glucosinolate / 493.111 / 0.61
1.00 / ± / 34.66 / 0.489896645 / ± / 15.31 / 0.08 / 10.28 / 476.1073 / 477.1146 / C16H31NO9S3 / 8-Methylthio-octyl glucosinolate / 477.1161 / -3.14
1.00 / ± / 21.93 / 0.846415346 / ± / 14.81 / 0.35 / 2.61 / 447.0537 / 448.0610 / C16H20N2O9S2 / glucobrassicin / 448.0611 / -0.22
1.00 / ± / 21.93 / 0.846415346 / ± / 14.81 / 0.35 / 2.14 / 447.0600 / 448.0673 / C16H20N2O9S2 / indol-3ylmethy glucosinolate / 448.061 / 14.06
LIPIDS
WT / RDS / lap3/Wt / RSD / p-value / RT / observed ion [M-H]- / natural ion / proposed formula / proposed annotation / proposed natural mass / ppm
1.00 / ± / 3.25 / 0.781409218 / ± / 6.61 / 0.00 / 23.10 / 279.2334 / 280.2407 / C18H32O2 / 10E,12Z-Octadecadienoic acid / 280.2402303 / 1.68
1.00 / ± / 6.92 / 0.728247783 / ± / 11.71 / 0.01 / 24.79 / 452.2763 / 453.2836 / C21H44NO7P / 1-16:0-lysophosphatidylethanolamine / 453.2855393 / -4.28
1.00 / ± / 16.97 / 0.680856438 / ± / 13.84 / 0.05 / 21.33 / 474.2649 / 475.2722 / C23H42NO7P / 1-18:3-lysophosphatidylethanolamine / 475.2698892 / 4.86
1.00 / ± / 0.75 / 1.290300921 / ± / 10.98 / 0.02 / 32.01 / 281.2488 / 282.2561 / C18H34O2 / Oleinic acid / 282.2558803 / 0.78
1.00 / ± / 4.73 / 1.033376582 / ± / 6.12 / 0.50 / 33.09 / 283.2641 / 284.2714 / C18H36O2 / 9-Octadecene-1,18-diol / 284.2715 / -0.35
1.00 / ± / 6.10 / 0.767175661 / ± / 2.51 / 0.02 / 23.12 / 277.2160 / 278.2233 / C18H30O2 / alpha-eleosteric acid / 278.2246 / -4.67
1.00 / ± / 16.89 / 0.670908047 / ± / 6.05 / 0.03 / 21.26 / 277.2165 / 278.2238 / C18H30O2 / alpha-linolenic acid / 278.2246 / -2.88
1.00 / ± / 16.80 / 1.503847452 / ± / 17.35 / 0.05 / 32.60 / 269.2478 / 270.2551 / C17H34O2 / Heptadecanoic acid / 270.2558803 / -2.89
MISCELLANEOUS
WT / RDS / lap3/Wt / RSD / p-value / RT / observed ion [M-H]- / natural ion / proposed formula / proposed annotation / proposed natural mass / ppm
1.00 / ± / 12.80 / n.d. / ± / 16.56 / 236.1046 / 237.1119 / C11H15N3O3 / 2-(4-carbamimidoyl-2-hydroxy-benzylamino)-propionic acid / 237.1113414 / 2.36
1.00 / ± / 0.07 / 0.915629423 / ± / 10.30 / 0.20 / 0.58 / 201.0256 / 202.0329 / C5H6N4O5 / 5-Hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate / 202.0338193 / -4.55
1.00 / ± / 11.51 / 0.7659581 / ± / 28.63 / 0.18 / 16.56 / 221.1530 / 222.1603 / C14H22O2 / Rishitin / 222.1620 / -7.65
UNKNOWN
WT / RDS / lap3/Wt / RSD / p-value / RT / observed ion [M-H]- / natural ion / proposed formula / proposed annotation / proposed natural mass / ppm
1.00 / ± / 24.00 / 0.61 / ± / 6.46 / 0.05 / 24.92 / 480.3076 / 481.3149
1.00 / ± / 19.68 / 0.57 / ± / 6.15 / 0.02 / 22.11 / 502.2932 / 503.3005
1.00 / ± / 10.67 / 0.68 / ± / 17.88 / 0.03 / 23.12 / 504.3082 / 505.3155
1.00 / ± / 1.05 / 0.68 / ± / 9.47 / 0.00 / 23.88 / 504.3109 / 505.3182
1.00 / ± / 22.74 / 0.57 / ± / 8.30 / 0.03 / 25.73 / 506.3210 / 507.3283
1.00 / ± / 45.05 / 3.06 / ± / 80.01 / 0.23 / 16.29 / 593.3311 / 594.3384
1.00 / ± / 49.95 / 3.77 / ± / 79.80 / 0.19 / 14.39 / 611.3420 / 612.3493
1.00 / ± / 14.05 / 44.45 / ± / 46.74 / 0.07 / 13.55 / 613.3565 / 614.3638
1.00 / ± / 26.10 / 0.68 / ± / 9.17 / 0.11 / 21.18 / 675.3573 / 676.3646
1.00 / ± / 12.95 / n.d. / ± / 12.79 / 703.4246 / 704.4319
1.00 / ± / 10.69 / n.d. / ± / 12.47 / 791.4803 / 792.4876
1.00 / ± / 22.26 / 0.74 / ± / 1.91 / 0.11 / 2.05 / 478.0875 / 479.0948
1.00 / ± / 9.17 / n.d. / ± / 12.17 / 879.5335 / 880.5408
1.00 / ± / 36.84 / 0.71 / ± / 1.23 / 0.24 / 3.32 / 492.1040 / 493.1113
1.00 / ± / 17.98 / 2.13 / ± / 77.72 / 0.31 / 17.05 / 575.3214 / 576.3287
1.00 / ± / 15.00 / 0.68 / ± / 19.88 / 0.05 / 23.73 / 476.2773 / 477.2846