Stem Cell Metabolism and Regenerative Medicine Group
Basic & Clinical Myology Laboratory
Department of Physiology,
The University of Melbourne,
Melbourne, Victoria, 3010 / Ph: +6138344-3672
Mob: +614 35-490-096
Email:
Web:
Twitter: @jgryall

Curriculum Vitae for Dr. James G. Ryall

Personal Information

NationalityAustralian

Date of Birth8th of August, 1979

Place of BirthMelbourne, VIC

Employment History

Jan 2015 – Present The University of MelbourneMelbourne, VIC

– Senior Research Fellow and Group Leader of the Stem Cell Metabolism and Regenerative Medicine Group in the Basic and Clinical Myology Laboratory

Oct 2012 – Dec 2014 The University of MelbourneMelbourne, VIC

– NH&MRC Biomedical Research Fellow in the Basic and Clinical Myology Laboratory

May 2010 – September 2012National Institutes of Health Bethesda, MD

– Visiting Research Fellow in the Laboratory of Muscle Stem Cells and Gene Regulation

May 2008 –May 2010National Institutes of Health Bethesda, MD

– NH&MRC Overseas Biomedical Research Fellow in the Laboratory of Muscle Stem Cells and Gene Regulation

Jan 2006 - May 2008The University of MelbourneMelbourne, VIC

– Research Officer in the Basic and Clinical Myology Laboratory

Nov 2005 - Jan 2006The University of MelbourneMelbourne, VIC

– Research Assistant in the Basic and Clinical Myology Laboratory

Education

2010 AprilCold Spring Harbor LaboratoryCold Spring Harbor, NY

– Attendee at the two-week long course “Molecular Embryology of the Mouse”

2002-2005University of MelbourneMelbourne, VIC

– Ph.D. (Thesis entitled “Age-related changes in skeletal and cardiac muscle structure and function: effects of 2-agonist administration”)

– Awarded the Chancellors Prize for excellence in a PhD thesis (Medicine, Dentistry and Health Sciences)

1998-2001University of MelbourneMelbourne, VIC

– B.Sc. (Hons) in Physiology (Thesis entitled “Comparison of the efficacy of clenbuterol and fenoterol on the functional properties of mammalian skeletal muscle”)

– Awarded first class honours and a place on the Deans Honours List for receiving the top mark in Physiology Honours

Appointments and Services to the Discipline

– Member of the inaugural School of Biomedical Sciences Early/Mid-Career Research Association steering committee.

–Editor for an issue of Methods in Molecular Biology entitled “Skeletal Muscle Development” (2017).

–Guest editor for a special edition of the journal Stem Cells International, entitled “Epigenetic Regulation Shapes the Stem Cell State” (2016).

– Grant reviewer for the National Health and Medical Research Council (NH&MRC, 2013, 2015), Australian Research Council (ARC, 2015, 2016), Telethon Foundation (Italy, 2012,2013), the Dutch Parent Project (2010)and theMaryland Technology Development Corporation (TEDCO, 2009).

– Reviewer for numerous international journals, including: Journal of Clinical Investigation, Genes & Development, EMBO Journal, Human Molecular Genetics, American Journal of Physiology: Cell Physiology, PLOS One, British Journal of Pharmacology,Journal of Applied Physiology, Experimental Physiology, Muscle Nerve,Clinical and Experimental Physiology and Pharmacology.

– Basic and Clinical Myology Laboratory webpage maintainer (2006-2008)

– President of the Physiology Postgraduate Student Society (2004–05)

– Postgraduate representative on the Department of Physiology safety committee (2003-04)

– Treasurer of the Physiology Postgraduate Student Society (2002–03)

Professional Affiliations

– Australian Physiological Society (AuPS)

– Society for Developmental Biology (SDB)

– Australian Society for Stem Cell Research (ASSCR)

– International Society for Stem Cell Research (ISSCR)

Awards and Grants

2016 – Recipient of a National Health and Medical Research Council Project Grant (2017-2020, $831,652) entitled “A simple method to improve stem cell transplant therapy”

2015 – Awarded $45,000 via The University of Melbourne Research Grant Support Scheme for the project “Strategies to improve success rates of stem cell transplant therapy”

2014 – Recipient of an Australian Research Council Discovery Project (2015-2019, $733,796) entitled “Understanding the cellular cues that direct muscle stem cell specification”

2012– Received the New Investigator Recognition Award from the Lymn Foundation at the Function, Development, and Repair of the Muscle Cell meeting held in NY for the project entitled “The NAD+ Dependent Sirtuin SIRT1 Maintains Quiescence of Adult Skeletal Muscle Stem Cells”

2010– Recipient of a Fellows Award for Research Excellence (FARE) from the National Institutes of Health. A $1,000 travel prize awarded for the project entitled “Sirtuin1 is essential for successful mammalian skeletal muscle growth and development”.

2009– Recipient of a Fellows Award for Research Excellence (FARE) from the National Institutes of Health. A $1,000 travel prize awarded for the project entitled “Understanding the role of Sirtuin1 in skeletal muscle growth and development”.

2008– Awarded the A.K. McIntyre Medal by the Australian Physiological Society. This award is presented once a year to the post-doctoral researcher adjudged to have made outstanding contributions to Australian Physiological Research.

– Recipient of a (CJ Martin) Biomedical Overseas Research Fellowship from the National Health and Medical Research Council of Australia ($313,848).

2007– Awarded the Chancellors Prize for excellence in a PhD thesis (Medicine, Dentistry and Health Sciences). This prize is awarded to one PhD graduate each year from the Faculty of Medicine, Dentistry and Health Sciences, and Veterinary Science at The University of Melbourne.

2006– Early Career Research Grant, $37,485 awarded by The University of Melbourne for the project entitled “The role of -adrenergic signalling in regenerating skeletal muscle”.

2005 – RM Gibson scientific research award, $6,000 awarded by the Australian Association of Gerontology.

– Australian Physiological Society award for the best paper published in a refereed journal by a postgraduate student (Ryall et al. 2004).

2004– Travel grant to the value of $200 to attend the 2nd meeting of Australian Health and Medical Research Congress, held in Sydney.

– Melbourne Abroad Travelling Scholarship, $900 awarded by The University of Melbourne for travel to the US to attend a conference and to collaborate with Dr. Paul Gregorevic at the University of Washington, Seattle.

– Caroline tum Suden/Frances A. Hellebrandt professional opportunity award for meritorious research, USD$500 awarded by the American Physiological Society at the 2004 meeting of Experimental Biology, held in Washington DC.

2003 – Clinical and Experimental Pharmacology and Physiology prize for best oral communication, at the 70th annual meeting of the Australian Physiological and Pharmacological Society, held in Sydney.

– Travel grant to the value of $250 to attend the 70th annual meeting of the Australian Physiological and Pharmacological Society, held in Sydney.

– Recipient of a Postgraduate Biomedical Research Scholarship from the National Heart Foundation of Australia.

2002 – Clinical and Experimental Pharmacology and Physiology prize for best oral communication, at the 69th annual meeting of the Australian Physiological and Pharmacological Society, held in Melbourne.

– Recipient of an Australian Postgraduate Award for postgraduate study in the Department of Physiology, at The University of Melbourne.

– Deans List Award for receiving the top mark in final year Physiology (Honours), 2001.

2001–Recipient of R.D. Wright Summer Studentship by the Department of Physiology at the University of Melbourne.

Invited Seminars

2016 – “Metabolism regulates asymmetric division and commitment to the myogenic lineage inskeletal muscle stem cells”FASEB Science Research Conference on Skeletal Muscle Satellite Cells and Regeneration, Colorado, USA

2015– “Metabolic reprogramming in skeletal muscle stem cells” Scientific Meeting of the Australian Physiological Society, Hobart, TAS

– “Metabolism regulates proliferation and asymmetric division of skeletal muscle stem cells” Baker IDI, Melbourne, VIC

2014– “A career in academia, why?”Students of Pharmacology Society Seminar, The University of Melbourne, VIC

– “Metabolic reprogramming in skeletal muscle stem cells”COMBIO 2014, Canberra, ACT

–“Metabolic reprogramming in skeletal muscle stem cells” Seahorse Bioscience User Group Meeting, Gold Coast, QLD

–“How the extracellular metabolic environment can alter intracellular gene expression and change stem-cell fate”Berlin-Brandenburg School for Regenerative Therapies, InternationalSummer Schoolon Innovative Approaches in Regenerative Therapies, Berlin, Germany

– “Metabolism dictates decisions in skeletal muscle stem-cell fate” Gage Muscle Conference, Canberra, ACT

2013–“Metabolic Reprogramming of skeletal muscle stem cells” Monash University, Department of Physiology Seminar Series, Melbourne, VIC

– “Metabolic Reprogramming of skeletal muscle stem cells” The University of Melbourne, Department of Physiology Seminar Series, Melbourne, VIC

2011– “The role of SirT1 in the maintenance of satellite cell quiescence” Exercise, Muscle & Metabolism, Melbourne, VIC

–“Sirtuin1 is essential for successful mammalian skeletal muscle development” NIAMS Intramural Research Program Annual Retreat, Bethesda, MD

2009– “Understanding the role of Sirtuin1 in mammalian skeletal muscle growth and development” National Institutes of Health Research Festival, Bethesda MD

2008– “A novel role for -adrenoceptor signalling in the regulation of skeletal muscle mass” 74th Scientific Meeting of the Australian Physiological Society, Melbourne VIC

2007– “Role of 2-adrenoceptor signalling in skeletal muscle - Implications for muscle wasting and disease” Department of Molecular Biology, The University of Texas South Western Medical Centre at Dallas, TX.

2005– “-adrenergic regulation of muscle repair and ageing” Exercise, Muscle & Metabolism, Melbourne VIC

Journal Publications

H-index=24Lifetime Citations=2044

  1. Ly CH & Ryall JG (2017). Measuring mitochondrial substrate utilization in skeletal muscle stem cells. Methods in Molecular Biology (in press).
  2. Ryall JG (2017). Simultaneous measurement of mitochondrial and glycolytic activity in quiescent muscle stem cells. Methods in Molecular Biology (in press).
  3. Mølck C, Ryall J, Failla LM, Coates J, Pascussi JM, Heath J, Stewart G & Hollande F (2016). The A2b adenosine receptor antagonist PSB-603 promotes oxidative phosphorylation and ROS production in colorectal cancer cells via adenosine receptor-independent mechanism. Cancer Letters383, 135-143.
  4. Giraud J, Failla LM, Pascussi JM, Lagerqvist EL, Ollier J, Finetti P, Bertucci F, Ya C, Gasmi I, Bourgaux JF, Prudhomme M, Mazard T, Ait-Arsa I, Houhou L, Birnbaum D, Pelegrin A, Vincent C, Ryall JG, Joubert D, Pannequin J & Hollande F (2016). Autocrine progastrin secretion promotes the tumorigenic potential, survival, and self-renewal of colon cancer stem cells. Cancer Research76, 3618-3628.
  5. Davey JR, Watt KI, Parker BL, Chaudhuri R, Ryall JG, Cunningham L, Qian H, Sartorelli V, Sandri M, Chamberlain J, James DE, Gregorevic P (2016). Integrated expression analysis of muscle hypertrophy identifies Asb2 as a negative regulator of muscle mass. JCI Insight1, e85477.
  6. Caretti G, Berghella L, Juan A, Latella L, Ryall J(2016). Epigenetic regulation shapes the stem cells state. Stem Cells International2016, 8143407.
  7. Laker RC & Ryall JG (2016). DNA methylation in skeletal muscle stem cell specification, proliferation and differentiation. Stem Cells International2016, 5725927.
  8. Ryall JG, Cliff T, Dalton S &Sartorelli V (2015). Metabolic reprogramming of stem cell epigenetics. Cell Stem Cell17, 651-662.
  9. Ryall JG, Dell’Orso S, Derfoul A, Juan A, Zare H, Feng X, Clermont D, Koulnis M, Gutierrez-Cruz G, Fulco M & Sartorelli V (2015). The NAD+-dependent SIRT1 deacetylase translates a metabolic switch into regulatory epigenetics in skeletal muscle stem cells. Cell Stem Cell16, 171-183. * Citations (Ct)=19
  10. Rowlands BD, Lau CL, Ryall JG, Thomas DA, Klugmann M, Beart PM & Rae CD (2015). SIRT1 modulators SRT1720 and resveratrol have distinctly different effects on brain metabolism. Journal of Neuroscience93, 1147-1156.
  11. Church JE, Trieu J, Sheorey R, Chee AY, Naim T, Baum DM, Ryall JG, Gregorevic P & Lynch GS (2014). Functional β-adrenoceptors are important for early muscle regeneration in mice through effects on myoblast proliferation and differentiation.PLoS One9, e101379.
  12. Koopman R, Ly CH & Ryall JG (2014). A metabolic link to skeletal muscle wasting and regeneration. Frontiers in Physiology 5, 32. * Ct=12
  13. Proserpio V, Fittipaldi R, Ryall JG, Sartorelli V Caretti G (2013). The methyltransferase SMYD3 mediates the recruitment of transcriptional cofactors at the myostatin and c-Met genes and regulates skeletal muscle atrophy. Genes & Development 27, 1299-1312. * Ct=14
  14. Ryall JG (2013). Metabolic reprogramming as a novel regulator of skeletal muscle development and regeneration. FEBS Journal280, 4004-4013. * Ct=8
  15. Peserico A, Chiacchiera F, Grossi V, Matrone A, Latorre D, Simonatto M, Fusella A, Ryall JG, Finley LW, Haigis MC, Villani G, Puri PL, Sartorelli V & Simone C (2013). A novel AMPK-dependent FoxO3A-SIRT3 intramitochondrial complex sensing glucose levels. Cellular and Molecular Life Sciences70, 2015-2029. * Ct=33
  16. Ryall JG (2012). The role of sirtuins in the regulation of metabolic homeostasis in skeletal muscle. Current Opinion in Clinical Nutrition & Metabolic Care15, 561-566. * Ct=10
  17. van Der Poel C, Gosselin LE, Schertzer JD, Ryall JG, Swiderski K, Wondemaghen M & Lynch GS (2011). Ageing prolongs inflammatory marker expression in regenerating rat skeletal muscles after injury. Journal of Inflammation 29, 41. * Ct=8
  18. Juan AH, Ryall JG*, DerfoulA*, Feng X*, Dell’Orso S*, Pasut A*, ZareH, Simone J, Rudnicki MA & Sartorelli V (2011). Polycomb Ezh2 controls self renewal and safeguards transcriptional identity of skeletal muscle stem cells. Genes & Development 25, 789-794. * Ct=82*These authors contributed equally to this work
  19. Murphy KT, Cobani V, Ryall JG, Krasney PA, Ibebunjo C & Lynch GS (2011). Acute antibody-directed myostatin inhibition attenuates disuse muscle atrophy and weakness in mice. Journal of Applied Physiology 110, 1065-1072 * Ct=21
  20. Ryall JG*, Murphy KT*, Snell SM, Nair L, Krasney PA, Ibebunjo C, Holden KS, Koopman R & Lynch GS (2010). Antibody-directed myostatin inhibition improves diaphragm in young, but not adult dystrophic mdx mice. American Journal of Pathology176, 2425-2434. * Ct= 34*These authors contributed equally to this work
  21. Ryall JG, Church JE & Lynch GS (2010). A novel role for -adrenoceptor signalling in skeletal muscle growth, development and regeneration. Clinical and Experimental Physiology and Pharmacology37, 397-401. * Ct=16
  22. Pearen MA, Ryall JG, Lynch GS & Muscat GEO (2009). Expression profiling of skeletal muscle following acute and chronic 2-adrenergic stimulation: Implications for hypertrophy, metabolism and circadian rhythm. BMC Genomics10, 448. * Ct=31
  23. Koopman R, Church JE, Ryall JG & Lynch GS (2009). The role of -adrenoceptor signaling in skeletal muscle: therapeutic implications for muscle wasting disorders. Current Opinion in Clinical Nutrition and Metabolic Care12, 601-606. * Ct=16
  24. Turpin SM, Ryall JG, Southgate RJ, Darby I, Hevener AL, Febbraio MA, Kemp BE, Lynch GS & Watt MJ (2009). Examination of 'lipotoxicity' in skeletal muscle of high-fat fed and ob/ob mice. Journal of Physiology587, 1593-1605. * Ct=46
  25. Dzamko N, Schertzer JD, Ryall JG, Steel R, Macaulay L, Wee S, Chen Z, Michell BJ, Oakill J, Watt MJ, Beck-Jorgensen S, Lynch GS, Kemp BE & Steinberg GR (2008). AMPK independent pathways regulate fatty acid oxidation during muscle contraction. Journal of Physiology586, 5819-5831. * Ct=100
  26. Gehrig SM, Ryall JG,Schertzer JD& Lynch GS (2008). IGF-I analogue protects muscles of dystrophic mdx mice fromcontraction-mediated damage. Experimental Physiology93, 1190-1198. * Ct=23
  27. Ryall JGLynch GS (2008). The potential and the pitfalls of the development of 2-adrenoceptor agonists as drugs for the management of muscle wasting. Pharmacology & Therapeutics120, 219-232. * Ct=53
  28. Ryall JG,Schertzer JD, Murphy KT, Allen AM & Lynch GS (2008). Chronic 2-adrenoceptor stimulation impairs cardiac relaxation via reduced SR Ca2+-ATPase activity. American Journal of Physiology; Heart and Circulatory Physiology294, H2587-H2595. * Ct=21
  29. Ryall JG,Schertzer JD, Alabakis TM, Gehrig SM, Plant DR & Lynch GS (2008). Intramuscular 2-agonist administration enhances early regeneration and functional repair after myotoxic injury inrat skeletal muscle. Journal of Applied Physiology105, 165-172. * Ct=21
  30. Pearen MA, Myers SA, Raichur S, Ryall JG, Lynch GSMuscat GEO (2008). The orphan nuclear receptor, NOR-1, a target of -adrenergic signaling, regulates gene expression that controls oxidative metabolism in skeletal muscle. Endocrinology 149, 2853-2865. * Ct=95
  31. Lynch GS, Schertzer JD & Ryall JG (2008).Anabolic agents for improving muscle regeneration and function after injury. Clinical and Experimental Pharmacology and Physiology35, 852-858. * Ct=25
  32. Ryall JG,Schertzer JD & Lynch GS (2008). Cellular and molecular mechanisms underlying age-related skeletal muscle wasting and weakness. Biogerontology9, 213-228. * Ct=155
  33. Lynch GS & Ryall JG (2008). Role of -adrenoceptor signaling in skeletal muscle – implications for muscle wasting and disease. Physiological Reviews 88, 729-767. * Ct=201
  34. Schertzer JD, Gehrig SM, Ryall JG & Lynch GS (2007). Modulation of IGF-I and IGFBP interactions enhances skeletal muscleregeneration and ameliorates the dystrophic pathology in mdx mice. American Journal of Pathology171, 1180-1188. * Ct=35
  35. Ryall JG,Schertzer JD & Lynch GS (2007). Attenuation of age-related muscle wasting and weakness in ratsafter formoterol treatment: therapeutic implications for sarcopenia. Journals of Gerontology: Series A62, 813-823. * Ct=42
  36. Lynch GS, Schertzer JD & Ryall JG (2007). Therapeutic approaches for muscle wasting disorders. Pharmacology & Therapeutics113, 461-487. *Ct=121
  37. Harcourt LJ, Schertzer JD, Ryall JG & Lynch GS (2007). Low dose formoterol administration improves muscle function in dystrophic mdx mice without increasing fatigue. Neuromuscular Disorders17, 47-55. *Ct=39
  38. Pearen MA, Ryall JG, Maxwell MA, Ohkura N,Lynch GSMuscat GEO (2006). The orphan nuclear receptor, nor-1, is a target of β-adrenergic signaling in skeletal muscle.Endocrinology147, 5217-5227. *Ct=92
  39. Schertzer JD, Ryall JG& Lynch GS (2006). Systemic administration of IGF-1 enhances oxidative status and reduces contraction induced injury in skeletal muscles of mdx mice.American Journal of Physiology; Endocrinology and Metabolism291, E499-E505. *Ct=56
  40. Ryall JG, Sillence MN & Lynch GS (2006). Systemic administration of 2-adrenoceptor agonists, formoterol and salmeterol, elicit skeletal muscle hypertrophy in rats at micromolar doses. British Journal of Pharmacology147, 587-595. * Ct=71
  41. Ryall JG,* Gregorevic P,* Plant DR, Sillence MN & Lynch GS(2005). Chronic 2-agonist administration affects cardiac function of adult but not old rats, independent of -adrenoceptor density. American Journal of Physiology; Heart and Circulatory Physiology289, 344-349. *Ct=20

*These authors contributed equally to this work

  1. Schertzer JD, Plant DR, Ryall JG, Beitzel F, Stupka N & Lynch GS (2005). 2-agonist administration increases sarcoplasmic reticulum Ca2+-ATPase activity in aged rat skeletal muscle. American Journal of Physiology; Endocrinology and Metabolism288, 526-533. *Ct=31
  2. Ryall JG, Plant DR, Gregorevic P, Sillence MN & Lynch GS (2004). 2-Agonist administration reverses muscle wasting and improves muscle function in aged rats. Journal of Physiology555, 175-188. * Ct=83
  3. Ryall JG, Plant DR & Lynch GS (2004). Making old muscles young again: a therapeutic role for 2-agonists? Physiology News56, 33-35. *Ct=3
  4. Beitzel F, Gregorevic P, Ryall JG, Plant DR, Sillence MN & Lynch GS (2004). 2-Adrenoceptor agonist fenoterol enhances functional repair of regenerating rat skeletal muscle following injury. Journal of Applied Physiology96, 1385-1392. * Ct=73
  5. Ryall JG, Gregorevic P, Plant DR, Sillence MN & Lynch GS(2002). 2-agonist fenoterol has greater effects on contractile function of rat skeletal muscles than clenbuterol. American Journal of Physiology; Regulatory, Integrative and Comparative Physiology 283, 1386-1394. * Ct=69

Book Chapters

  1. Ryall JG & Lynch GS (2010). Role of -adrenoceptor signalling in skeletal muscle wasting: Implications for sarcopenia In: Sarcopenia- Age-Related Muscle Wasting and Weakness: Mechanisms and Treatments pp. 449-472.

Teaching Experience

2016–Supervisor of a Ph.D. student, The University of Melbourne

Hai Ly“Metabolic reprogramming as a master regulator of skeletal muscle stem cell identity”

–Supervisor of a Ph.D. student, The University of Melbourne

Savant Thakur“The role of heat shock proteins in the regulation of skeletal muscle regeneration”

–Supervisor of aB.Sc. (honours) student, The University of Melbourne

Claudia Gemes“A MondoA-TXNIP-glycolysis pathway regulates skeletal muscle stem cell specification and differentiation” (final mark - H1, 81%)

2015–Supervisor of a Ph.D. student, The University of Melbourne

Hai Ly“Metabolic reprogramming as a master regulator of skeletal muscle stem cell identity”

–Supervisor of a Ph.D. student, The University of Melbourne

Savant Thakur“The role of heat shock proteins in the regulation of skeletal muscle regeneration”

–Supervisor of aB.Sc. (honours) student, The University of Melbourne

Alexandra Webster“The master metabolic regulator pyruvate kinase directly regulates myogenic proliferation and differentiation” (final mark - H1, 92%, Awarded a place on the Deans Honours List for the top mark in Physiology Honours)

–Supervisor of twoB.Sc. students (third year), The University of Melbourne

2014–Supervisor of a Ph.D. student, The University of Melbourne

Hai Ly“Metabolic reprogramming as a master regulator of skeletal muscle stem cell identity”

–Supervisor of aB.Sc. (honours) student, The University of Melbourne

Mi-Diem Phung“Glycolysis can regulate skeletal muscle stem cell proliferation and differentiation in a MondoA-TXNIP dependent manner”(final mark - H1, 91%, Awarded a place on the Deans Honours List for the top mark in Physiology Honours)

–Supervisor of twoB.Sc. students (third year), The University of Melbourne

Angus Pritchard“Myogenic cell proliferation is reduced following electron transport chain inhibition” (final mark - H1, 89%)

Timothy Khoo “Inhibition of Enolase with sodium fluoride reduces the proliferative capacity of C2C12 myoblasts” (final mark - H1, 84%)

2013–Supervisor of aB.Sc. (honours) student, The University of Melbourne

Hai Ly“The role of metabolic reprogramming in skeletal muscle stem cells: Implications for stem cell transplant therapies”(final mark - H1, 91%, Awarded a place on the Deans Honours List for the top mark in Physiology Honours)