November 2, 2016
Explore Life’s True Nature with Super-Resolution and Nanoscopy
Leica Microsystems paves the way to access imaging beyond the diffraction limit with HyVolution 2 and the Leica TCS SP8 STED ONE
Mannheim, Germany. Withthe Super-Resolution Technology HyVolution 2 and the Leica TCS SP8 STED ONE Nanoscope, Leica Microsystems expandsits productportfolio for light microscopyhaving resolutionbeyondthe diffraction limit. With these technologies, scientists inneurobiology, cancer research,and immunologycanvisualizemolecular interactionsand structures in livingcells in order to understand life’s true nature. With HyVolution 2 and the Leica TCS SP8 STED ONE, Leica Microsystemsoffers researchersand imaging facilities attractive solutionsforgetting started usingsuper-resolution and nanoscopy.
“During the last 20 years,super-resolution and nanoscopy have revolutionized researchon functionalinteractions insidecellsat the molecular level. This is also reflected in the Nobel Prize for Chemistry of2014“, says Markus Lusser, President of Leica Microsystems.“These technologies arewell on their way to establish themselves as standardfluorescence methods forfundamental biomedical research.On the basis of ourproven TCS SP8 platform,we now offer acomplete portfolio forconfocalimagingbeyond the diffraction limit:from HyVolution 2 forsuper resolution at the touch of a button –tothe TCS SP8 STED ONE for getting started in3D-Nanoscopy–to oursuccessful TCS STED 3X for multi-color STED imaging over the full spectral range.Leica Microsystems is theonlycompanywhich has more than 12 years of experiencein developingcommercialimaging systemsthat can resolve beyond the diffraction limit.“
Withthe HyVolution 2 Super-ResolutionTechnologyallowingresolutiondown to 140 nanometers,processesand structurescan be resolved in severalcolorssimultaneouslywith high acquisition speed and high signal-to-noise ratio. HyVolution 2, which is available for all existingand new Leica TCS SP8 systems, combineshighly sensitive Leica HyD HybridDetectors,the Leica TCS SP8 spectral detectionsystem,seamlesslyintegrated Huygens deconvolutionsoftware,and high speed graphic processors.
The Leica TCS SP8 STED ONE Nanoscope achieves a resolution down to 30 nanometers. The system revealsmorphologyand dynamics inside cells at the molecular level– in 2D and 3D – andallows the co-localization ofmoleculesto be reliably quantified. For differentapplications,theSTED ONEis available either witha 592 or 660 STED laser. The spectral detectionsystem combinessensitivitywith the Leica HyDsand freely selectableexcitation wavelengthof thewhite light laserto make theLeica TCS SP8 STED ONE a flexible imaging system.
More information on HyVolution 2:
More information on Leica TCS SP8 STED ONE:
On Science Lab, the knowledge portal around microscopy by Leica Microsystems, readers will find articles, interviews, and webinars on super-resolution and nanoscopy:
Caption:Super-Resolution: COS-7 cells acquired with HyVolution 2. Sample courtesy of Dr. Jana Doehner, Center of Microscopy and Image Analysis, University of Zurich, Switzerland.
Caption: Nanoscopy: multi-color STEDimage: vimentin, Alexa 647 (red), alpha-tubulin, Alexa 594 (green), F-actin, Alexa 488 phalloidin (cyan). Confocal: DAPI (blue). Courtesy of Dr. Eugene Katrukha, Utrecht University, The Netherlands.
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About Leica Microsystems
Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures. Ever since the company started as a family business in the nineteenth century, its instruments have been widely recognized for their optical precision and innovative technology.It is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopes.
Leica Microsystems has seven major plants and product development sites around the world. The company is represented in over 100 countries, has sales and service organizations in 20 countries, and an international network of distribution partners. Its headquarters are located in Wetzlar, Germany.
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