Primersequences used in touch down PCR for multiamplicon analysis with all the considered DHPLC approaches

Forward Primer / Reverse Primer / Amplicon lenght
BRCA1ex1 / CCATCCTCTGATTGTACCTTGATT / AGCTTCGGAAATCCACTCTC / 306 bp
BRCA1int1 / AGCTGACAGATGGGTATTCTTTGA / GCACCTCTTCTTCCACAAGGT / 498 bp
BRCA1ex2 / CGCCGAATGAAGTTGTCATTTTATAAACCTTTT / GACATGTCTTTTCTTCCCTAGTATGT / 270 bp
BRCA1ex3 / ACTCAGTCATAACAGCTCAAAGTT / CTTCTATAAAGTTAGGTGTTTCCT / 247 bp
BRCA1ex4 / CCACATTGTTCAAAGAGATAGAATG / CATGTATAAAGGTTGTTAACTAAAC / 442 bp
BRCA1ex5 / CTTGTAATTCACCTGCCATTAC / GAATGGTTTTATAGGAACGCTATG / 313 bp
BRCA1ex6 / GGTTTTCTACTGTTGCTGCATCTT / TTTTCATGGACAGCACTTGAG / 256 bp
BRCA1ex7A / AACACAACAAAGAGCATACATAGG / AAGAAGAAGAAAACAAATGGTTTTAC / 269 bp
BRCA1ex7B / CGCCCGCCGCTTTCCCTTGTATTTTACAGATGC / CAGGAGGACTGCTTCTAGCC / 279 bp
BRCA1ex8 / CATGGTGTCAAGTTTCTCTTCAG / CGCCCGCCGCCCACAAATACAAATTATGACCAAGA / 293 bp
BRCA1ex9 / CGCCCGCCGCTCTGTATTACATACTAGCTTAACTA / CGCCGAAATAGGAAAATACCAGCTTCATAGA / 266 bp
BRCA1ex10 / TTGGTCAGCTTTCTGTAATCG / TCTACCCACTCTCTTTTCAGT / 239 bp
BRCA1ex11A / ACCTCCAAGGTGTATGAAGTATGT / TTCCTTACTTCCAGCCCATCT / 388 bp
BRCA1ex11B / GCATGAGAACAGCAGTTTATTAC / ATTTGGAGTGAACTCTTTCACTTT / 500 bp
BRCA1ex11C / TGAGTGGTTTTCCAGAAGTGA / GGGTTAGGATTTTTCTCATTCTG / 561 bp
BRCA1ex11D / GAGCGTCCCCTCACAAATAA / TGACTGGCATTTGGTTGTACT / 499 bp
BRCA1ex11E / CGCCCGCCGCTCAGTAGAAATCTAAGCCCACCTAAT / CGCCCGCCGCATCTTTCAGTTTGCAAAACCCTTT / 428 bp
BRCA1ex11F / CGCCCGCCGCAATGCACCTGGTTCTTTTACTAAGTG / CGCCGACTGACTCACACATTTATTTGGTTCT / 319 bp
BRCA1ex11G / TACCTGGTACTGATTATGGCACTCAG / TAGAGCCTCCTTTGATACTACATT / 537 bp
BRCA1ex11H / GGAAATGCAGAAGAGGAATGTG / TCTCTAATGTTATTACGGCTAATTGTG / 473 bp
BRCA1ex11I / GCGGCTAGAGGAAAACTTTGAGGAACATTCA / GCGGCTTCAGGATGCTTACAATTACTTCCA / 336 bp
BRCA1ex11J / TTCAAGCAGAACTAGGTAGAAACAGA / TTGCTAAAAACAGCAGAACTTTCCTT / 334 bp
BRCA1ex11K / CGCCCGCCGCTTTGTTCTGAGACACCTGATGACC / CGCCCGCCGCTTACCAAATAACAAGTGTTGGAAGC / 285 bp
BRCA1ex11L / CATACACATTTGGCTCAGGGTTA / TGGAAGAACCAATCAAGAAAGGAT / 382 bp
BRCA1ex11M / ATCACCTTAGTGAGGAAACAAAATG / TACTTGGAAATTTGTAAAATGTGCTC / 346 bp
BRCA1ex 12 / GTAAATCCAGTCCTGCCAATG / GTCAGCAAACCTAAGAATGTG / 273 bp
BRCA1 ex 13 / GCGGGCGGCGTGGAAAGCTTCTCAAAGTATTTCAT / GCGGGCGGCGTGTTGGAGCTAGGTCCTTACTCTT / 337 bp
BRCA1 ex 14 / ACTATCAGAACAAAGCAGTAAAG / ACAAAAGAAGTATCCTAGAGCAA / 325 bp
BRCA1 ex 15 / TTCACAATTGGTGGCGATG / TGTAGGATTCAGAGTAAAATCAAAGTGTT / 294 bp
BRCA1 ex 16 / CGCCCGCCGCTGTAATTCAACATTCATCGTTGTGTA / CGCCCGCCGCACAATACCTACATAAAACTCTTTCCAG / 459 bp
BRCA1 ex 17 / TCTGAGCTGTGTGCTAGAGGTAA / CCTCGCCTCATGTGGTTTTATG / 205 bp
BRCA1 ex 18 / TGCTGGTTATAATTAGTGGTGTT / GGTGTAAAAATGCAATTCTGAGG / 314 bp
BRCA1 ex 19 / AAAGAGCACGTTCTTCTGCTG / TGTGCATTGTTAAGGAAAGTGG / 200 bp
BRCA1 ex20 / TGTCTGCTCCACTTCCATTGAA / TCTTCCACTTATCAGCTAAGATCT / 428 bp
BRCA1 ex21 / CTAGATTTTCCTTCTCTCCATTC / GTAGAGAAATAGAATAGCCTCTA / 242 bp
BRCA1 ex 22 / GGGCCTGGGTTAAGTATGC / AGTCTTGCTCACAGGAGAGAAT / 194 bp
BRCA1 ex 23 / AGTCCTACTTTGACACTTTGAATGC / CACCAGGTAATGAGTGATAAACCAA / 182 bp
BRCA1 ex 24 / TCCTAAGAACTCATACAACCAG / AACAACAGCCTGAATAGAAAGA / 557 bp
BRCA2 ex 1 / CCTGACTTCCGGGTGGTG / AGAGACAAAAGGGCAAGAAGC / 370 bp
BRCA2 ex2 / GCGGCTGAGTTTTACCTCAGTCACATAATAAGG / GCGGCTTTTTAGAAAACACTTTCTCGGTGTA / 244 bp
BRCA2 ex3 / TGTCTGTCACTGGTTAAAACTAAG / TAGTTTGTAGTTCTCCCCAGTC / 337 bp
BRCA2 ex4 / AAACACTTCCAAAGAATGCAAAT / ATACAGAAAACCAGCCAATTCAA / 409 bp
BRCA2 ex 5/6 / AAAATAACCTAAGGGATTTGCTTTG / CTCAGGGCAAAGGTATAACG / 294 bp
BRCA2 ex7 / TCATACAGGCAATTCAGTAAACG / CAACCTCATCTGCTCTTTCTTG / 272 bp
BRCA2 ex8 / GTAGATGTGCTTTTTGATGTCTG / ACATATAGGACCAGGTTTAGAGAC / 281 bp
BRCA2 ex 9 / GGGGACTACTACTATATGTGCATTG / TTAAGAAAAGAGGAGCAATCCTTC / 499 bp
BRCA2 ex 10A / TTCTATGAGAAAGGTTGTGAGAATAA / CAGTATCATTTGGTTCCACTTCAGATA / 378 bp
BRCA2 ex 10B / GCGGGCGGCGCAGTTGTAGATACCTCTGAAGAAGATAGT / GCGGCGGTCACATGAAGAAATATGCAA / 384 bp
BRCA2 ex 10C / AAGGAAGTTGTACCGTCTTTGG / TATTAAACCTGCATTCTTCAAAGCTAC / 588 bp
BRCA2 ex 10D / CTGAAAGTGGACTGGAAATACA / CATGTTTGAGTGACCTGATTC / 483 bp
BRCA2 ex 11A / GATGGTACTTTAATTTTGTCACTTTG / ACTAGGTTTGACAGAACATCCTT / 480 bp
BRCA2 ex 11B / GGAAGGACAGTGTGAAAATGAT / GGATTGACAGTTATTTTTGAAATTG / 492 bp
BRCA2 ex 11C / TTATAAAAACGTTGAGCTGTTGC / CTTCTTAATGTTATGTTCAGAGAGCTTGAT / 598 bp
BRCA2 ex 11D / GACTCTTAGGTCCAATTTCAAATCA / ATTCAAACTGACTTCCTGATTCTTCTAATA / 396 bp
BRCA2 ex 11E / GCGGCGATGTTATTTTCCAAGCAGGATTTT / CGGGCGGGGCTTTTGTTACAGTCATTTTTCAACAGG / 356 bp
BRCA2 ex 11F / TGAGGAATGCAGAGATGCTGA / CAGAATCATGACATTTACTTGAAGA / 338 bp
BRCA2 ex 11G / TTTCTACTGAAGCTCTGCAAAAAG / CCCCGCCGTGAATACAAACAGTATCATTTTTACTTGAAT / 372 bp
BRCA2 ex 11H / TGAAAATGAAGATAACAAATATACTG / TCAGAATTTAAGGAAAAGTTATGCAA / 444 bp
BRCA2 ex 11I / TCAGACTGCAAGTGGGAAAAATA / GTGATTTCACTAGTACCTTGCT / 387 bp
BRCA2 ex 11J / CTGAAAGAAAGTGTCCCAGTT / GACTGAATAAGGGGACTGATTT / 519 bp
BRCA2 ex 11K / GTAGACAAACTGAAAATCTCAAAACA / GAGCTAGTCACAAGTTCCTCAA / 589 bp
BRCA2 ex 11L / GGTATTGAGCCAGTATTGAAGA / CCAAACTAACATCACAAGGTG / 547 bp
BRCA2 ex 11M / GAAAATTATGGCAGGTTGTTAC / TCCACTTGCTGTACTAAATCCA / 529 bp
BRCA2 ex 11N / CCAAGCAAGTCTTTTCCAAAGTA / CAGAAAAAGTTTCAGTTTTACCAA / 585 bp
BRCA2 ex 11O / AGGTGGTTCTTCAGAAAATAATCA / AAACACTTTAAAAATAGTGATTGGCAAC / 478 bp
BRCA2 ex12 / GACTTTACTCTTTCAAACATTAGGTCAC / CGGAAGATCCGCATAACTCAG / 560 bp
BRCA2 ex13 / TGCTGATTTCTGTTGTATGCTTG / CATTTATAAAAACGAGACTTTTCTC / 356 bp
BRCA2 ex 14A / AATAGTATCACCATGTAGCAAATGAG / TTAGTTTTAAAAGGTGGAACAAAGAC / 335 bp
BRCA2 ex 14B / GCGGCAGTTTCAGGACATCCATTTTATCAAG / GCGGCGAAAACCATCAGGACATTATTTAACA / 399 bp
BRCA2 ex15 / CAGGGGTTGTGCTTTTTAAATTTC / CCATTCCTGCACTAATGTGTTC / 355 bp
BRCA2 ex16 / TTTGGTAAATTCAGTTTTGGTTTGT / AGGGATGAGGGAATACATAAAAGT / 340 bp
BRCA2 ex17 / GCGGGCGGCGTTTGTACAGAGAATAGTTGTAGTTGTTGAA / CAGTGTGGGATGGCAACTGTC / 392 bp
BRCA2 ex18 / TCAGTTTTTATTCTCAGTTATTCAGTGAC / GTACATCTAAGAAATTGAGCATCCTT / 561 bp
BRCA2 ex 19 / GGCAGTTCTAGAAGAATGAAAAC / GCAAGAGACCGAAACTCCA / 341 bp
BRCA2 ex20 / CCTGATACAATTAACTTGAATGTT / AGTCTCTAAGACTTTGTTCTCATA / 271 bp
BRCA2 ex 21 / GGTGTTTTATGCTTGGTTCTTTAG / CATTTCAACATACTCCTTCCTGT / 303 bp
BRCA2 ex22 / CAATAGATGGAACTTTTTTGTTCTG / GTAGTGGATTTTGCTTCTCTGATA / 384 bp
BRCA2 ex23 / ATCACTTCTTCCATTGCATCTTT / CAACATATGGAGATTCCATAAACT / 276 bp
BRCA2 ex24 / CAGTTTTGATAAGTGCTTGTTAGT / AGCTCCAACTAATCATAAGAGATT / 290 bp
BRCA2 ex25 / CCTTTCTTGCATCTTAAAATTCATCT / ACTACCTCAACTATCCAATTTGTAT / 417 bp
BRCA2 ex26 / TGGGTTTGCAATTTATAAAGCAG / GATGGCCTCCATATATACTTCTTA / 244 bp
BRCA2 ex 27A / ACTGTGTGTAATATTTGCGTGCTT / GGAGTCATCTGAGGAGAATTCAGT / 433 bp
BRCA2 ex 27B / AAAGAACTGCAAAAAGAGAAGAGC / ATAAACTGGAAAGGTTAAGCGTCA / 523 bp
BRCA2 ex 27C / CGCCGTCTGATCAAAGAACAGGAGAGTTC / CGCCGCACCGAGCTCAGCCAAAG / 363 bp
BRCA2 ex 27D / CCCCGCCGTTGCTTCAGTTGCATATCTTAAAACT / CGCCGAAGGAGAACTATTTCATAGTGAGTTACC / 421 bp
BRCA2 ex28 / CTATTCCAGTGTGATCTCTGAA / GGACGTAACAAATGAGAGTATCAT / 270 bp