Table S2: Summary of metabolite ions that showed significant changes in sera of Lateolabrax japonicus. Ion rank from a loading S-plot of OPLS-DA analysis showed the rank of ions with the highest confidence and greatestcontribution to separation between the first and the last group of Lateolabrax japonicus samples collected on October 9 andOctober 22, 2007, respectively. Ion rank is the same as labeling of ions with relevant m/z in Figure 5b. Fold changeequals the fold increase in the level observed in the last group compared with the first group ( Cov. and Cor. >0) or the fold decrease in the level observed in the last group compared with the first group ( Cov. and Cor. <0). VIP (Variable Importance in the Projection), p value (P value of Independent T Test ), Cor.(correlation), Cov.(covariance) and factor of change of a metabolite were obtained from the OPLS-DA analysis by use of the data sets in positive ion scan mode or negative ion scan mode corresponding to positive ion or negative ion of relevant adduct in the third column respectively.

RT (min)_m/z / Identity / Adduct * / Empirical element composition / Mass error (ppm) / VIP / p value / Cor. / Cov. / Factor of Change
Phosphatidylcholine
PC with HUFA at the sn-1 position
11.58_852.5534 / 20:5/22:6 / [M+H]+ / C50H79NO8P / 1.1 / 9.017 / 1.49×10-10 / 0.784 / 0.261 / 1.98
11.58_874.5344 / [M+Na]+ / C50H78NO8PNa / 2.2 / 7.789 / 6.64×10-8 / 0.709 / 0.225 / 1.59
11.46_896.5462 / [M+HCOOH-H]- / C51H79NO10P / 2.2 / 6.392 / 4.74×10-6 / 0.824 / 0.165 / 1.34
11.47_836.5262 / [M-CH3]- / C49H75NO8P / 3.8 / 4.949 / 1.42×10-6 / 0.838 / 0.128 / 1.36
11.84_878.5715 / 22:6/C22:6 / [M+H]+ / C52H81NO8P / 1.7 / 7.209 / 9.62×10-12 / 0.855 / 0.209 / 2.01
11.86_900.5538 / [M+Na]+ / C52H80NO8PNa / 2.1 / 7.278 / 2.93×10-10 / 0.802 / 0.211 / 1.69
11.70_922.5620 / [M+HCOOH-H]- / C53H81NO10P / 2.4 / 5.842 / 2.58×10-7 / 0.801 / 0.151 / 1.37
11.70_862.5407 / [M-CH3]- / C51H77NO8P / 2.3 / 3.896 / 1.36×10-6 / 0.789 / 0.101 / 1.34
12.10_854.5734 / 20:4/22:6 / [M+H]+ / C50H81NO8P / 4 / 4.466 / 6.50×10-11 / 0.806 / 0.129 / 1.82
12.10_876.5531 / [M+Na]+ / C50H80NO8PNa / 1.4 / 3.729 / 2.51×10-6 / 0.655 / 0.108 / 1.41
11.98_898.5619 / [M+HCOOH-H]- / C51H81NO10P / 1.2 / 3.637 / 1.01×10-5 / 0.751 / 0.094 / 1.28
11.82_828.5583 / 20:4/20:5 / [M+H]+ / C48H79NO8P / 4.8 / 2.853 / 1.19×10-3 / 0.576 / 0.081 / 1.42
PC with SFA or MUFA at the sn-1 position
13.06_780.5544 / 16:0/20:5 / [M+H]+ / C44H79NO8P / 0.1 / 6.218 / 1.35×10-3 / 0.455 / 0.18 / 1.26
13.58_854.5660 / 18:1/22:6 / [M+Na]+ / C48H82NO8PNa / 1.9 / 4.737 / 1.08×10-3 / -0.487 / -0.137 / 1.25
13.48_806.5724 / 16:0/22:6 / [M+H]+ / C46H81NO8P / 3 / 4.356 / 3.03×10-2 / 0.369 / 0.126 / 1.07
13.12_850.5629 / [M+HCOOH-H]- / C47H81NO10P / 3.6 / 5.885 / 2.51×10-2 / -0.317 / -0.152 / 1.24
13.14_790.5414 / [M-CH3]- / C45H77NO8P / 3.4 / 4.989 / 1.46×10-2 / -0.334 / -0.129 / 1.27
12.26_778.5386 / 16:1/20:5 / [M+H]+ / C44H77NO8P / 0.1 / 4.114 / 8.09×10-4 / 0.495 / 0.119 / 1.27
14.20_804.5517 / 16:1/22:6 / [M+H]+ / C46H79NO8P / 3.2 / 3.233 / 4.39×10-2 / -0.286 / -0.094 / 1.26
15.98_804.5770 / 16:0/18:1 / [M+HCOOH-H]- / C43H83NO10P / 2 / 3.176 / 1.11×10-3 / -0.623 / -0.082 / 2.77
PC with plasmalogen at the sn-1 position
14.52_814.5707 / p-16:0/22:5 / [M+Na]+ / C46H82NO7PNa / 2.3 / 7.24 / 1.50×10-4 / -0.583 / -0.21 / 1.50
14.54_792.5902 / [M+H]+ / C46H83NO7P / 0.6 / 4.501 / 8.82×10-4 / -0.493 / -0.13 / 1.38
14.36_836.5828 / [M+HCOOH-H]- / C47H83NO9P / 2.6 / 5.891 / 1.06×10-4 / -0.676 / -0.152 / 1.30
14.36_776.5603 / [M-CH3]- / C45H79NO7P / 1.2 / 4.904 / 2.12×10-5 / -0.73 / -0.127 / 1.32
14.51_840.5905 / p-18:0/22:6 / [M+Na]+ / C48H84NO7PNa / 2.6 / 2.833 / 4.24×10-4 / -0.574 / -0.08 / 1.26
Lysophosphatidyl choline
6.69_568.3403 / 22:6 / [M+H]+ / C30H51NO7P / 0.0 / 3.526 / 3.7×10-10 / 0.783 / 0.102 / 1.71
6.59_552.3082 / [M-CH3]- / C29H47NO7P / 1.4 / 8.938 / 4.18×10-13 / 0.865 / 0.231 / 2.00
6.59_612.3306 / [M+HCOOH-H]- / C31H51NO9P / 0.8 / 3.239 / 1.96×10-10 / 0.820 / 0.084 / 1.95
5.97_526.2932 / 20:5 / [M-CH3]- / C27H45NO7P / 0.4 / 4.474 / 1.41×10-12 / 0.882 / 0.116 / 2.29
6.71_528.3084 / 20:4 / [M-CH3]- / C27H47NO7P / 1.1 / 3.296 / 1.43×10-12 / 0.841 / 0.085 / 2.13
Cholic acid metabolite
2.69_554.2540 / taurocholic acid / [M+K]+ / C26H45NO7SK / 2.5 / 3.865 / 6.08×10-2 / -0.455 / -0.112 / 1.97
2.70_514.2826 / [M -H]- / C26H44NO7S / 2.5 / 3.467 / 2.41×10-2 / -0.32 / -0.09 / 1.81