Fayetteville State University

College of Arts and Sciences

Department of Natural Sciences

Spring 2005

I. LOCATOR INFORMATION

Course No. & Name / CHEM 310-01 Instrumental Method of Analysis
Semester Hours of Credit / 4
Lecture time / TBA
Instructor / Cevdet Akbay, Ph.D.
Office Location / LS 227
Office Phone / (910) 672-1943
E-mail /
Web Address / http://faculty.uncfsu.edu/cakbay/index.htm
Office Hours / MW: 11:30am–12:30pm; T: 10:00am–12:00 pm & 2:00–4:00 pm; Th: 2:00-4:00 pm

II. COURSE DESCRIPTION

The application of electrochemical, spectroscopic, and chromatographic methods to qualitative and quantitative analysis.

III. TEXTBOOK

Skoog, West, Holler & Nieman. Principles of Instrumental Analysis, Fifth Edition, Brooks/ColeThomson Learning,1998

IV. COURSE OBJECTIVES

Most chemical analyses today are done using instrumentation rather than traditional “wet”methods (gravimetry, titration), due to the advantage of speed. Some analyses, especially those involving separation and spectroscopy, cannot be done without instrumentation. This course will provide an understanding of the usefulness of analytical instrumentation in everyday life, healthcare, and industry. Although designed primarily for chemistry majors, students who have pursued careers in biology, environment, forensics, or healthcare have also found the material in this course to be useful.

V. COURSE COMPETENCIES

1. Communications skills in technical writing, gained through writing laboratory reports in the style of a journal article.

2. The use of computers in analytical chemistry to process data and generate graphs.

3. Familiarity with the ways in which concentration is expressed, including the pH scale.

4. Proficiency with electrochemical concepts, including the relationship between voltage and Gibbs free energy, and the Nernst equation.

5. Statistical analysis of data to determine the concentration of unknowns and to estimate uncertainty of measurements.

6. Knowledge of the theory and, when possible, familiarity of the use of the four main groups of analytical instruments:

a. Atomic spectroscopy (absorption, emission, fluorescence)

b. Molecular spectroscopy (UV/Vis, luminescence, IR, Raman, NMR, mass spectroscopy)

c. Electroanalytical methods (potentiometry, coulometry, voltammetry)

d. Separations techniques (GC, HPLC)

VI. EVALUATION CRITERIA

The progress of each student will be evaluated by means of three one-hour examinations given during the semester, laboratory reports, a term paper, and a final examination. Make-up on exams is administered based on university policy.

A. Grade distribution

Three hour exams (15 × 3) 45

Laboratory reports 20

Final 20

Term Paper 15

Total 100

B. Grading scale

The final letter grade assigned to the student will be based upon the following numerical

equivalencies.

A = 90 – 100

B = 80 – 89

C = 70 – 79

D = 60 – 69

F = Below 59

VII. COURSE OUTLINE

Week
/
Chapter
/
Topic
Jan 6th / 1,2 / Introduction, Circuits, basics
Jan 10th / 2 / Resistive circuits, electrical components
Jan 17th
MLK Day / 3, 4 / Operational amplifiers, digital computers
Jan 24 / 5, 6 / Signal to noise ratio, Spectrometric methods
Jan 31st / 13 , Exam#1 / UV/Vis absorption spectroscopy
Feb 7th / 13, 14 / Components of optical instrument & Applications of UV/Vis
Feb 14th / 15 / Luminescence spectroscopy
Feb 21th / 16, Midterm / Introduction to infrared (IR) spectroscopy
Feb 28th
---Mar 5th / Spring Break
Mar 7th / 17, 18 / Application of IR spectroscopy, Raman spectroscopy
Mar 14th / 19, 20 / Nuclear magnetic spectroscopy and mass spectroscopy
Mar 21th / 8-12 / Introduction and application of atomic spectroscopy
Mar 28th / 22, 25 Exam #3 / Electroanalytical introduction and Voltammetry
April 4th / 26 / Introduction to chromatography
April 11st / 27 / Gas chromatography
April 18th / 28 / High performance liquid chromatography
April 18th / 29 / Supercritical fluid chromatography
April 25 th / 30 / Capillary electrophoresis
April 27th / Final Exam / 10:00 am-11:50 am

VIII. COURSE REQUIREMENTS

A. Hour exams are in the short-essay format. Questions may consist of either a numerical problem to work out or a concept to explain in a few sentences. To receive full credit for numerical problems, make sure your thought process is written out clearly.

B. Laboratory reports. Each laboratory experiment has three components.

a. Pre-lab: Read the procedure beforehand. Write it out in your own words, and bring that procedure to lab. Write out the procedure as step-by-step instructions and make a list of equipment and chemicals at the top, with the hazards associated with each of the chemicals.

b. In lab: Use your laboratory notebook to record what you have done, not what you plan to do. Record raw data in the notebook as you see them.

c. Laboratory report: This is to have a title, author(s), abstract, introduction, experimental, results and discussion, and reference(s). Further details will be given.

IX. TEACHING STRATEGIES

A directed study course lends itself best to the discussion format. Questions are encouraged. Many instrument makers make any given type of instrument, and the particular software and features will differ. Therefore, to maximize the use of this course, I will focus on the theory and phenomenon behind the instrument, pointing out what they have in common and what you might expect when you come across instruments that differ from what we have in the laboratory.

X. BIBLIOGRAPHY

Horst Friebolin. Ein- und Zweidimensionale NMR-Spektroskopie., 3rd Ed. Translated by Jack Becconsall. Wiley, 1998. I highly recommend this little book as a guide to NMR

spectroscopy.

Norman B. Colthup, Lawrence H. Daly, Stephen E. Wiberly. Introduction to Infrared and Raman Spectroscopy, 3rd ed. Academic, 1990.

XI. DISCLAIMER

To accommodate emergent circumstances, the professor reserves the right to make reasonable changes in the syllabus while the course is in progress. Any understandings between a student and the professor including, but not limited to, changes, expectations, or modifications to course requirements or procedures must be in writing and must be signed by both parties. Any question of interpretation of course requirements or of understandings between a student and the professor will be at the discretion of the professor.

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