Position / Sequence / Exp. mass / From seq.
G1:U12-A58:C75 / 5'-TpGCCCGGAUAGCU-3' 5'-pAGUCCCUGUUCGGGCGCC-3' / 9825.17 / 9825.19
G1:C13-G59:C75 / 5'-TpGCCCGGAUAGCUC-3' 5'-pGUCCCUGUUCGGGCGCC-3' / 9801.15 / 9801.18
G1:A14-A58:C75 / 5'-TpGCCCGGAUAGCUCA-3' 5'-pAGUCCCUGUUCGGGCGCC-3' / 10459.26 / 10459.28
G1:G5 / 5'-TpGCCCG-3' / 1783.16 / 1783.16
G1:C11 / 5'-TpGCCCGGAUAGC-3' / 3742.42 / 3742.43
G1:A14 / 5'-TpGCCCGGAUAGCUCA-3' / 4682.54 / 4682.55
C2:A26 / 5'-pCCCGGAUAGCUCAGUCGGUAGAGCA-3' / 8112.07 / 8112.09
G10:A14 / 5'-pGCUCA-3' / 1608.21 / 1608.22
C11:C17 / 5'-pCUCAGUC-3' / 2219.28 / 2219.29
C17:U33 / 5'-pCGGUAGAGCAUCAGACU-3' / 5526.73 / 5526.75
G18:A26 / 5'-pGGUAGAGCA-3' / 2996.41 / 2996.42
G18:U33 / 5'-pGGUAGAGCAUCAGACU-3' / 5221.70 / 5221.71
G19:A26 / 5'-pGUAGAGCA-3' / 2651.37 / 2651.38
G22:A26 / 5'-pGAGCA-3' / 1671.25 / 1671.25
A26:C49 / 5'-pAUCAGACUUUUAAUCUGAGGGUCC-3' / 7690.96 / 7690.97
U27:U36 / 5'-pUCAGACUUUU-3' / 3161.36 / 3161.37
U27:C48 / 5'-pUCAGACUUUUAAUCUGAGGGUC-3' / 7056.88 / 7056.88
U35:C49 / 5'-pUUAAUCUGAGGGUCC-3' / 4830.60 / 4830.61
G45:C49 / 5'-pGGUCC-3' / 1624.21 / 1624.21
U55:C61 / 5'-pUCAAGUC-3' / 2243.29 / 2243.30
C56:C74 / 5'-pCAAGUCCCUGUUCGGGCGC-3' / 6105.77 / 6105.79
A58:G73 / 5'-pAGUCCCUGUUCGGGCG-3' / 5166.64 / 5166.66
A58:C74 / 5'-pAGUCCCUGUUCGGGCGC-3' / 5471.68 / 5471.70
A58:C75 / 5'-pAGUCCCUGUUCGGGCGCC-3' / 5776.72 / 5776.74
G59:U64 / 5'-pGUCCCU-3' / 1890.23 / 1890.23
G59:C74 / 5'-pGUCCCUGUUCGGGCGC-3' / 5142.63 / 5142.64
G59:C75 / 5'-pGUCCCUGUUCGGGCGCC-3' / 5447.66 / 5447.68
U60:U64 / 5'-pUCCCU-3' / 1545.18 / 1545.19
C61:G65 / 5'-pCCCUG-3' / 1584.20 / 1584.21
U66:C75 / 5'-pUUCGGGCGCC-3' / 3230.41 / 3230.42
b) tRNALys3 digestion by Mung bean nuclease overnight
Position / Sequence / Exp. mass / From seq.
G1:G10-G59:G73 / 5'-TpGCCCGGAUAG-3' 5'-pGUCCCUGUUCGGGCG-3' / 8274.99 / 8275.02
G1:G10-C61:G73 / 5'-TpGCCCGGAUAG-3' 5'-pCCCUGUUCGGGCG-3' / 7623.92 / 7623.92
G1:C11-G59:G73 / 5'-TpGCCCGGAUAGC-3' 5'-pGUCCCUGUUCGGGCG-3' / 8580.03 / 8580.03
G1:C11-U60:G73 / 5'-TpGCCCGGAUAGC-3' 5'-pUCCCUGUUCGGGCG-3' / 8234.98 / 8234.98
G1:C11-C61:G73 / 5'-TpGCCCGGAUAGC-3' 5'-pCCCUGUUCGGGCG-3' / 7928.96 / 7928.96
G1:U12-G59:G73 / 5'-TpGCCCGGAUAGCU-3' 5'-pGUCCCUGUUCGGGCG-3' / 8886.06 / 8886.06
G1:U12-U60:G73 / 5'-TpGCCCGGAUAGCU-3' 5'-pUCCCUGUUCGGGCG-3' / 8541.01 / 8541.01
G1:C4 / 5'-TpGCCC-3' / 1438.11 / 1438.11
G1:G5 / 5'-TpGCCCG-3' / 1783.16 / 1783.16
G1:G10 / 5'-TpGCCCGGAUAG-3' / 3437.38 / 3437.38
G1:C11 / 5'-TpGCCCGGAUAGC-3' / 3742.42 / 3742.43
G1:U12 / 5'-TpGCCCGGAUAGCU-3' / 4048.45 / 4048.45
G1:C13 / 5'-TpGCCCGGAUAGCUC-3' / 4353.49 / 4353.49
G1:A14 / 5'-TpGCCCGGAUAGCUCA-3' / 4682.54 / 4682.55
G10:A14 / 5'-pGCUCA-3' / 1608.22 / 1608.22
G18:A26 / 5'-pGGUAGAGCA-3' / 2996.42 / 2996.42
G19:A31 / 5'-GUAGAGCAUCAGA-3' / 4265.61 / 4265.60
A31:G46 / 5'-ACUUUUAAUCUGAGGG-3' / 5160.65 / 5160.64
U39:U54 / 5'-UCUGAGGGUCCAGGGU-3' / 5230.67 / 5230.67
U39:U55 / 5'-UCUGAGGGUCCAGGGUU-3' / 5536.70 / 5536.70
G45:C49 / 5'-GGUCC-3' / 1624.21 / 1624.21
A58:G73 / 5'-pAGUCCCUGUUCGGGCG-3' / 5166.65 / 5166.66
G59:G73 / 5'-pGUCCCUGUUCGGGCG-3' / 4837.60 / 4837.60
U60:G73 / 5'-pUCCCUGUUCGGGCG-3' / 4492.55 / 4492.56
C61:G65 / 5'-pCCCUG-3' / 1584.20 / 1584.21
U67:C72 / 5'-pUCGGGC-3' / 1969.26 / 1969.26
U67:G73 / 5'-p UCGGGCG -3' / 2314.30 / 2314.31
c) tRNALys3 digestion by V1 nuclease overnight
Position / Sequence / Exp. mass / From seq.
G1:U12 / 5'-TpGCCCGGAUAGCU-3' / 4048.44 / 4048.45
G1:A26 / 5'-TpGCCCGGAUAGCUCAGUCGGUAGAGCA-3' / 8617.06 / 8617.07
G1:U27 / 5'-TpGCCCGGAUAGCUCAGUCGGUAGAGCAU-3' / 8923.08 / 8923.10
C3:G10 / 5'-pCCGGAUAG-3' / 2627.36 / 2627.37
C4:U12 / 5'-pCGGAUAGCU-3' / 2933.38 / 2933.39
G6:U12 / 5'-pGAUAGCU-3' / 2283.30 / 2283.30
C11:A26 / 5'-pCUCAGUCGGUAGAGCA-3' / 5197.69 / 5197.70
C13:A26 / 5'-pCAGUCGGUAGAGCA-3' / 4586.63 / 4586.63
A14:C28 / 5'-pAGUCGGUAGAGCAUC-3' / 4892.65 / 4892.66
G15:A26 / 5'-pGUCGGUAGAGCA-3' / 3952.53 / 3952.54
C28:U39 / 5'-pCAGACUUUUAAU-3' / 3819.47 / 3819.48
C28:U41 / 5'-pCAGACUUUUAAUCU-3' / 4430.54 / 4430.54
A29:U39 / 5'-pAGACUUUUAAU-3' / 3514.43 / 3514.44
A29:A43 / 5'-pAGACUUUUAAUCUGA-3' / 4799.60 / 4799.60
G30:C40 / 5'-pGACUUUUAAUC-3' / 3490.42 / 3490.42
A31:A43 / 5'-pACUUUUAAUCUGA-3' / 4125.50 / 4125.50
C32:C40 / 5'-pCUUUUAAUC-3' / 2816.32 / 2816.32
C32:A43 / 5'-pCUUUUAAUCUGA-3' / 3796.44 / 3796.45
U33:C40 / 5'-pUUUUAAUC-3' / 2511.28 / 2511.28
U33:U67 / 5'-pUUUUAAUCUGAGGGUCCAGGGUUCAAGUCCCUGUU-3' / 11211.39 / 11211.37
C40:C49 / 5'-pCUGAGGGUCC-3' / 3254.42 / 3254.43
G42:C62 / 5'-pGAGGGUCCAGGGUUCAAGUCC-3' / 6843.90 / 6843.91
A43:G69 / 5'-pAGGGUCCAGGGUUCAAGUCCCUGUUCG-3' / 8717.10 / 8717.11
C49:C62 / 5'-pCAGGGUUCAAGUCC-3' / 4523.59 / 4523.60
A50:C62 / 5'-pAGGGUUCAAGUCC-3' / 4218.55 / 4218.56
A50:U67 / 5'-pAGGGUUCAAGUCCCUGUU-3' / 5786.71 / 5786.72
A50:C68 / 5'-pAGGGUUCAAGUCCCUGUUC-3' / 6091.75 / 6091.76
G51:C63 / 5'-pGGGUUCAAGUCCC-3' / 4194.54 / 4194.55
G51:U67 / 5'-pGGGUUCAAGUCCCUGUU-3' / 5457.67 / 5457.67
G52:C63 / 5'-pGGUUCAAGUCCC-3' / 3849.49 / 3849.50
G65:G71 / 5'-pGUUCGGG-3' / 2315.29 / 2315.29
G69:A76 / 5'-pGGGCGCCA-3' / 2642.37 / 2642.38
Table 2S:Digestion products obtained by treating human tRNALys3 with a)Mung bean nuclease for 5 h, b) overnight, and c) V1 nuclease overnight.Dashes are used to indicate duplex products. Monoisotopic masses are reported in mass units.
1.