Test and Spine Collagen Peptides Containing Hydroxyproline

Test and spine collagen peptides containing hydroxyproline

GLEAN_3 / Protein / Peptide / No. of HyP / Peptides
26008 / α1 collagen (1α) / T / 2586GSDGSPGPVGAPGPAGPSGQPGER2609 / 3 / 2 (2)
T / 2586GSDGSPGPVGAPGPAGPSGQPGER2609 / 4 / 2 (1)
T / 2673GSEGSQGQTGPPGVPGR2689 / 2 / 16
T / 2730GDQGNPGQPGAQGESGPLGPR2750 / 2 / 1
T
S / 2751GETGPAGPPGAQGESGER2768 / 1
1 / 12
1
T
S / 2772GSVGPAGPPGGVGER2786 / 1
1 / 8 (1)
2
T / 2787GPMGPPGMSGAPGAPGAK2804 / 3 / 6
T / 2849DGKPGPAGAPGEPGNSGPAGASGQR2873 / 3 / 3 (1)
T / 2874GLPGLVGLPGPQGQR2888 / 2 / 13 (2)
T / 2892GEDGGQGSPGAPGLTGEPGK2911 / 3 / 7
T / 2892GEDGGQGSPGAPGLTGEPGKR2912 / 3 / 6
T / 2912RGEPGVAGPPGPQGSAGER2930 / 2 / 10 (2)
T
S / 2913GEPGVAGPPGPQGSAGER2930 / 2
2 / 9 (1)
1
T / 3042GPPGPSGSPGPDGPAGAEGDR3062 / 2 / 4
T / 3096GDPGDQGPQGSPGSPGFAGPPGR3118 / 4 / 12
T / 3119SGNPGPQGELGPTGAR3134 / 1 / 12 (2)
T / 3135GETGGPGPSGPTGDPGPQGPLGAPGQQGER3164 / 3 / 4
T / 3210GEPGQSGSPGQPGLAGTTGPSGER3233 / 3 / 11
T / 3237GNDGQSGPPGPPGPTGPAGQSGILGLAGGSGPR3269 / 2 / 1
T / 3270GPGGPAGPPGEAGSR3284 / 1 / 3 (1)
T / 3270GPGGPAGPPGEAGSR3284 (see Fig. 1) / 2 / 1 (1)
T
S / 3363GETGSTGAPGPQGPTGAR3380 / 1
1 / 10
2
T / 3471GEGGSSGPPGPPGPPGPPGPPGQVVQSSYGVR3501 / 5 / 2
26009 / α2 collagen (2α) / T / 2023GEAGEGGNMGASGPVGAVGNPGQR2046 / 1 / 1 (1)
05167 / α5 collagen (5α) / T / 1513GAMAVGLPGSAGYAGTR1529 / 1 / 7 (1)
T / 1767GNPGLIGFVGVDGPR1781 / 1 / 7 (1)
T / 1839GTLGDNGEAGAAGTPGEAGTR1859 / 1 / 3
T / 1908GATGPPGQQGPPGLGGESGISGSR1931 / 2 / 4
T / 1941GEAGEEGEPGAAGQVGLPGSQGR1963 / 2 / 4
T / 2042QGEVGLTGSGGIGGPPGLR2060 / 1 / 5 (2)
T / 2061GLQGPPGLR2069 / 1 / 1 (1)
T / 2070GGVGPTGSMGEDGDPGVSGQQGSSGR2095 / 1 / 2
T / 2100GSQGPPGPTGPGGGR2114 / 1 / 5 (2)
T / 2148GIPGNPGPGGAPGER2162 / 3 / 11 (1)

T, test; S, spines. The prolines most likely hydroxylated according to the established collagen proline hydroxylation rules are in bold italics. Prolines confirmed to be hydroxylated by manual validation of selected spectra are underlined. The number of manually validated spectra of peptides is given in brackets after the total number of peptides with this sequence.