®HLA-A*26
August 2015101.424-12/12u
Rev. No: 00 74Y
Gel Documentation Form and Worksheet
HLA-A*26 (101.424-12/12u)Lot No:74YExpiry Date: 2018-February-01
Olerup SSP ABFor In Vitro Diagnostic Use
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®HLA-A*26
August 2015101.424-12/12u
Rev. No: 00 74Y
Name:______
Sample ID:______
DNA Conc.(ng/ul):______
Test Date:______
Tested By:______
Review Date:______
Reviewed By:______
Olerup SSP ABFor In Vitro Diagnostic Use
Franzengatan 5
SE-112 51 Stockholm
SwedenPage 1 of 8
®HLA-A*26
August 2015101.424-12/12u
Rev. No: 00 74Y
Interpretation:______Failed lanes: ______ Comments:______
Gel Picture
PHOTO DOCUMENT
Olerup SSP ABFor In Vitro Diagnostic Use
Franzengatan 5
SE-112 51 Stockholm
SwedenPage 1 of 8
®HLA-A*26
August 2015101.424-12/12u
Rev. No: 00 74Y
Olerup SSP ABFor In Vitro Diagnostic Use
Franzengatan 5
SE-112 51 Stockholm
SwedenPage 1 of 8
®HLA-A*26
August 2015101.424-12/12u
Rev. No: 00 74Y
‘ICB’ Internal Control Band,
‘AmpS’Amplicon Size
Olerup SSP ABFor In Vitro Diagnostic Use
Franzengatan 5
SE-112 51 Stockholm
SwedenPage 1 of 8
®HLA-A*26
August 2015101.424-12/12u
Rev. No: 00 74Y
Notes:
Product sizes are approximate. For detailed information, see the lot-specific Specificity Table and Interpretation Table.
This table is intended as a guide. For interpretation always use the Interpretation Table and/or Specificity Table.
HLA-specific PCR products shorter than 125 base pairs have a lower intensity and are less sharp than longer PCR products.
Primer mixes 1, 18 and 39 have a tendency to giving rise to primer oligomer formation.
Primer mixes 4, 17, 20, 22, 26 and 33 may have tendencies of unspecific amplifications.
Primer mixes 7 and 25 may give rise to a lower yield of HLA-specific PCR product than the other A*26 primer mixes.
In primer mix 4 the positive control band may be weaker than for other HLA-A*26 primer mixes.
Primer mix 48 contains a negative control, which will amplify more than 95% of HLA amplicons as well as the amplicons generated by the control primer pairs matching the human growth hormone gene. HLA-specific PCR product sizes range from 75 to 200 base pairs and the PCR product generated by the HGH positive control primer pair is 430 base pairs.
Olerup SSP ABFor In Vitro Diagnostic Use
Franzengatan 5
SE-112 51 Stockholm
SwedenPage 1 of 8
HLA-A*26
August 2015 101.424-12/12u
Rev. No: 00 74Y
Worksheet
Olerup SSP ABFor In Vitro Diagnostic Use
Franzengatan 5
SE-112 51 Stockholm
SwedenPage 1 of 8
HLA-A*26
August 2015 101.424-12/12u
Rev. No: 00 74Y
Worksheet
1HLA-A*26 alleles in bold lettering are listed as confirmed alleles on the on the IMGT/HLA web page release 3.20.0, April 2015.
2Alleles that have been deleted from or renamed in the official WHO HLA Nomenclature up to and including the last IMGT/HLA database release can be retrieved from web page
3Primer mix 9: Specific PCR fragment of 145 bp in the A*26:12 and 26:18 and in the A*02:309, 02:454, 25:28, 25:30, 31:03-31:04, 34:06 and 66:06 alleles. Specific PCR fragment of 190 bp in the A*26:29 and 26:49 and in the A*01:01:56 and 66:10 alleles.
Primer mix 10: Specific PCR fragment of 85 bp in the A*26:07:01-26:07:02 and in the 01:83:01-01:83:02 alleles. Specific PCR fragment of 260 bp in the A*26:20 and in the A*01:01:13 alleles. Specific PCR fragments of 85 and 260 bp in the A*02:146 allele.
Primer mix 12: Specific PCR fragment of 140 bp in the A*26:14, 26:18, 26:28, 26:52 and 26:73 and in the A*03:01:19, 25:09, 31:03-31:04, 34:03, 34:06 and 66:22 alleles. Specific PCR fragment of 240 bp in the A*26:16 and in the A*01:02, 01:20, 24:04, 24:109, 24:129, 29:37, 29:56, 30:57 and 30:90 alleles.
Primer mix 13: Specific PCR fragment of 110 bp in the A*26:45 and 26:106 alleles. Specific PCR fragment of 255 bp in the A*26:17 allele.
Primer mix 17: Specific PCR fragment of 105 bp in the A*26:74 allele. Specific PCR fragment of 175 bp in the A*26:09 and 26:91 and theA*02:309, 02:454, 03:01:19, 25:06, 31:03-31:04, 34:01:01-34:09 and 34:12 alleles.
Primer mix 18: Specific PCR fragment of 125 bp in the A*26:31and the A*01:60 and in the C*06:71 alleles. Specific PCR fragment of 205 bp in the A*26:21 allele.
Primer mix 19: Specific PCR fragment of 190 bp in the A*26:40 allele. Specific PCR fragment of 245 bp in the A*26:22 and theA*01:20w, 01:66w, 01:130w, 02:38, 02:101:01, 02:447, 02:543 and 66:09 alleles.
Primer mix 20: Specific PCR fragment of 60 bp in the A*26:27 allele. Specific PCR fragment of 210 bp in the A*26:23 and the 11:185 alleles
Primer mix 21:Specific PCR fragment of 115 bp in the A*26:24 and 26:99 and theC*02:140, 02:241, 31:48, 33:15 and 33:31alleles. Specific PCR fragment of 135 bp in the A*26:82allele. Specific PCR fragment of 205 bp in the A*26:41 allele.
Primer mix 22: Specific PCR fragment of 100 bp in the A*26:25N allele.Specific PCR fragment of 170 bp in the A*26:98 allele. Specific PCR fragment of 220 bp in the A*26:38 and in the C*06:75 allele.
Primer mix 23: Specific PCR fragment of 130 bp in the A*26:56 and 26:82 alleles. Specific PCR fragment of 165 bp in the A*26:26 allele.
Primer mix 26: Specific PCR fragment of 150 bp in the A*26:42 allele. Specific PCR fragment of 175 bp in the A*26:01:21,26:34 and 26:73 and theA*66:01:02 alleles.
Primer mix 27: Specific PCR fragment of 125 bp in the A*26:76 allele. Specific PCR fragment of 275 bp in the A*26:35 allele.
Primer mix 30: Specific PCR fragment of 120 bp in the A*26:15, 26:29, 26:56 and 26:99 and theA*02:140, 11:91:01, 31:48 and 33:15 and in the B*35:108:01, B*53:26 and C*12:91alleles. Specific PCR fragment of 235 bp in the A*26:33 allele.
Primer mix 32: Specific PCR fragment of 90 bp in the A*26:61 allele. Specific PCR fragment of 155 bp in the A*26:43:01 and theA*02:309, 02:454, 03:01:19, 25:19:01-25:19:02, 25:30, 31:03-31:04, 34:02:01, 34:02:03-34:04, 34:06-34:09 and 66:06alleles.
Primer mix 33: Specific PCR fragment of 105 bp in the A*26:46 allele. Specific PCR fragment of 170 bp in the A*26:98 allele. Specific PCR fragment of 200 bp in the A*26:53 allele.
Primer mix 34: Specific PCR fragment of 135 bp in the A*26:47 and the A*25:08 and 66:18 alleles. Specific PCR fragment of 190 bp in the A*26:29 and 26:49 and the A*01:01:56 and 66:10 alleles.
Primer mix 35: Specific PCR fragment of 110 bp in the A*26:48 and 26:69 and theA*34:01:01-34:01:02, 34:05 and 34:11-34:12alleles. Specific PCR fragment of 245 bp in the A*26:59 allele.
Primer mix 36: Specific PCR fragment of 125 bp in the A*26:54 and theA*02:454 and 25:05 alleles. Specific PCR fragment of 215 bp in the A*26:55 allele.
Primer mix 37: Specific PCR fragment of 245 bp in the A*26:50 allele. Specific PCR fragment of 410 bp in the A*26:64 and the A*68:89 allele.
Primer mix 38: Specific PCR fragment of 90 bp in the A*26:75 and theA*11:34 alleles. Specific PCR fragment of 190 bp in the A*26:51 and the A*01:168 alleles.
Primer mix 39: Specific PCR fragment of 220 bp in the A*26:63 and the A*24:181 alleles. Specific PCR fragment of 410 bp in the A*26:62, 26:64 and 26:72 and the A*23:09, 24:129 and 68:89 alleles.
Primer mix 43: Specific PCR fragment of 90 bp in the A*26:107N allele. Specific PCR fragment of 175 bp in the A*26:103 and theA*25:18alleles.
4The A*26:28 and A*26:52 alleles will give rise to identical amplification patterns with the HLA-A*26 subtyping kit.
5The following HLA-A*26 alleles can be distinguished by the different sizes of the HLA-specific PCR product:
Alleles / Primer mix / Alleles / Primer mixA*26:17, 26:106 / 13 / A*26:46, 26:53 / 33
A*26:23, 26:27 / 20 / A*26:51, 26:75 / 38
A*26:24, 26:41 / 21 / A*26:54, 26:55 / 36
A*26:25N, 26:38 / 22 / A*26:59, 26:69 / 35
A*26:43:01, 26:61 / 32 / A*26:62, 26:63 / 39
The HLA-A*26 subtyping kit cannot distinguish the following silent mutations: A*26:01:01-26:01:20 and 26:01:22-26:01:38 alleles, the A*26:02:01-26:02:02 alleles or the A*26:07:01-26:07:02 alleles.
‘w’, may be weakly amplified.
Olerup SSP ABFor In Vitro Diagnostic Use
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